EKS-ART-00513

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EKS-ART-00513

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
Other/WEE	241.8	1.4E-71	249	494	246

Status

Reviewed

Ensembl Protein

AT1G02970.1

UniProt Accession

Q8L4H0; Q9SRY9;

Protein Name

Wee1-like protein kinase

Protein Synonyms/Alias

Wee1-At;

Gene Name

WEE1

Gene Synonyms/Alias

WEE1; At1g02970; F22D16.3;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
AT1G02970	AT1G02970.1	AT1G02970.1

Organism

Arabidopsis thaliana

Functional Description

Cell cycle regulatory kinase that is not rate-limitingfor cycle progression under normal growth conditions. Transcriptionally activated upon DNA stress or damage in an ATR- or ATM-dependent manner. Once activated, inhibits plant growth by arresting dividing cells in the G2 phase before proceeding into mitosis. Down-regulates CDKA-1 and CDKD-2 by tyrosine phosphorylation. May target principally CDKA-1.

Protein Length

500

Protein Sequence
(FASTA)

MFEKNGRTLL AKRKTQGTIK TRASKKIRKM EGTLERHSLL QFGQLSKISF ENRPSSNVAS 60

SAFQGLLDSD SSELRNQLGS ADSDANCGEK DFILSQDFFC TPDYITPDNQ NLMSGLDISK 120

DHSPCPRSPV KLNTVKSKRC RQESFTGNHS NSTWSSKHRV DEQENDDIDT DEVMGDKLQA 180

NQTERTGYVS QAAVALRCRA MPPPCLKNPY VLNQSETATD PFGHQRSKCA SFLPVSTSGD 240

GLSRYLTDFH EIRQIGAGHF SRVFKVLKRM DGCLYAVKHS TRKLYLDSER RKAMMEVQAL 300

AALGFHENIV GYYSSWFENE QLYIQLELCD HSLSALPKKS SLKVSEREIL VIMHQIAKAL 360

HFVHEKGIAH LDVKPDNIYI KNGVCKLGDF GCATRLDKSL PVEEGDARYM PQEILNEDYE 420

HLDKVDIFSL GVTVYELIKG SPLTESRNQS LNIKEGKLPL LPGHSLQLQQ LLKTMMDRDP 480

KRRPSARELL DHPMFDRIRG 500

Nucleotide Sequence
(FASTA)

ATGTTCGAGA AGAACGGAAG AACACTGTTG GCGAAGAGGA AAACCCAAGG GACAATCAAA 60

ACCAGGGCAT CGAAGAAGAT TCGGAAGATG GAAGGGACAT TGGAGCGTCA CTCTCTGCTT 120

CAATTCGGTC AATTGTCGAA GATTTCTTTC GAAAATCGTC CGTCGTCGAA TGTTGCTTCA 180

TCGGCGTTTC AGGGTCTCCT GGATTCGGAT TCTTCGGAAC TCCGAAATCA GTTGGGTTCC 240

GCTGATTCAG ATGCCAATTG CGGAGAGAAG GACTTTATTC TTAGCCAAGA CTTCTTCTGC 300

ACACCTGACT ATATAACCCC GGACAATCAG AACTTGATGA GCGGCTTAGA TATCAGCAAG 360

GATCATTCTC CATGTCCTAG GTCTCCTGTT AAACTAAATA CAGTTAAAAG CAAAAGATGT 420

CGCCAGGAGA GTTTCACAGG AAATCATTCA AATTCTACCT GGTCTTCAAA ACATAGAGTA 480

GATGAACAAG AGAATGATGA TATTGACACA GATGAGGTGA TGGGGGATAA ACTCCAAGCT 540

AATCAAACAG AGAGGACTGG ATACGTTTCA CAGGCTGCAG TTGCTCTGCG GTGTCGGGCT 600

ATGCCACCCC CTTGCCTCAA GAATCCGTAT GTGTTGAATC AGTCTGAGAC TGCTACTGAC 660

CCTTTTGGAC ATCAGAGATC AAAATGTGCA AGTTTTCTCC CTGTAAGTAC AAGTGGGGAT 720

GGCTTGTCAA GATATCTCAC GGACTTTCAT GAAATCCGGC AAATTGGTGC TGGACATTTC 780

AGTCGGGTAT TTAAGGTGTT GAAGAGAATG GACGGTTGCC TATATGCTGT GAAACACAGC 840

ACAAGAAAGT TGTATCTAGA TTCAGAGAGA CGTAAAGCTA TGATGGAAGT GCAAGCTCTT 900

GCTGCTCTTG GGTTTCATGA AAATATAGTA GGATATTACT CCTCGTGGTT TGAAAATGAG 960

CAATTATACA TTCAACTGGA ACTCTGCGAT CACAGCTTGT CAGCTTTGCC TAAGAAATCT 1020

TCTCTTAAAG TCTCAGAAAG AGAGATCTTG GTGATTATGC ATCAGATAGC AAAGGCATTA 1080

CATTTTGTGC ATGAGAAAGG AATAGCTCAT TTAGATGTAA AACCTGACAA TATTTACATT 1140

AAGAACGGTG TTTGCAAGCT TGGTGACTTT GGTTGTGCCA CACGATTGGA CAAAAGCTTA 1200

CCAGTAGAAG AAGGAGATGC ACGTTACATG CCTCAAGAAA TTCTAAATGA AGACTACGAA 1260

CACCTTGATA AAGTCGATAT CTTCTCTTTA GGTGTGACGG TTTATGAGCT GATTAAGGGA 1320

TCTCCTCTTA CAGAATCAAG AAACCAGTCG CTCAATATCA AAGAAGGAAA ACTTCCTCTC 1380

CTTCCTGGCC ATTCGTTGCA GTTACAACAA CTTCTTAAGA CAATGATGGA TCGTGATCCG 1440

AAGCGTCGGC CTTCTGCTAG AGAATTACTG GACCATCCCA TGTTTGATAG GATTCGAGGT 1500

TGA 1503

Domain Profile

S: 1     FeevekiGkGefgevfkvvkredgklyAikkskkplkglskekkalrEvtahavlgqhen  60

         F+e+++iG G+f+ vfkv kr dg+lyA+k+s ++l   s++ ka+ Ev+a a lg hen

Q: 249   FHEIRQIGAGHFSRVFKVLKRMDGCLYAVKHSTRKLYLDSERRKAMMEVQALAALGFHEN  308

         899*********************************************************

S: 61    vvryisaWeeddslyiqtElCeggsLeaalqeceakkkaleeaelkkilldlllglrylH  120

         +v y+s+W e+++lyiq ElC ++sL+a     ++++ +++e e+  i+ ++ ++l+++H

Q: 309   IVGYYSSWFENEQLYIQLELC-DHSLSAL---PKKSSLKVSEREILVIMHQIAKALHFVH  364

         *********************.9****99...55666789********************

S: 121   ssslvHlDikpenifitkevtlklgDlGlvtelnsedkskveEGDsrylakEilqeeysk  180

         ++ + HlD+kp+ni+i+ ++++klgD+G +t+l  +++  veEGD+ry+++Eil+e+y++

Q: 365   EKGIAHLDVKPDNIYIK-NGVCKLGDFGCATRL--DKSLPVEEGDARYMPQEILNEDYEH  421

         ***************96.689************..78889********************

S: 181   l.kaDiFslgltilevaanvelPsnGeaWhklRkgklpdeilkelsedlrellklmlepd  239

         l k DiFslg+t+ e+    +l +  ++  ++++gklp  +l   s +l++llk+m++ d

Q: 422   LdKVDIFSLGVTVYELIKGSPLTESRNQSLNIKEGKLP--LLPGHSLQLQQLLKTMMDRD  479

         **************************************..********************

S: 240   perRptaeellkhev  254

         p+rRp+a+ell+h++

Q: 480   PKRRPSARELLDHPM  494

         ************986

Domain Sequence
(FASTA)

FHEIRQIGAG HFSRVFKVLK RMDGCLYAVK HSTRKLYLDS ERRKAMMEVQ ALAALGFHEN 60

IVGYYSSWFE NEQLYIQLEL CDHSLSALPK KSSLKVSERE ILVIMHQIAK ALHFVHEKGI 120

AHLDVKPDNI YIKNGVCKLG DFGCATRLDK SLPVEEGDAR YMPQEILNED YEHLDKVDIF 180

SLGVTVYELI KGSPLTESRN QSLNIKEGKL PLLPGHSLQL QQLLKTMMDR DPKRRPSARE 240

LLDHPM 246

Keyword

ATP-binding; Cell cycle; Cell division; Complete proteome; Kinase; Magnesium; Metal-binding; Mitosis; Nucleotide-binding; Nucleus; Phosphoprotein; Reference proteome; Transferase; Tyrosine-protein kinase.

Sequence Source

Ensembl

Orthology

Ortholog group

Arabidopsis lyrata"; ?>Brachypodium distachyon"; ?>Brassica rapa"; ?>Chlamydomonas reinhardtii"; ?>Cyanidioschyzon merolae"; ?>Glycine max"; ?>Hordeum vulgare"; ?>Musa acuminata"; ?>Oryza brachyantha"; ?>Oryza glaberrima"; ?>Oryza indica"; ?>Oryza sativa"; ?>Physcomitrella patens"; ?>Populus trichocarpa"; ?>Selaginella moellendorffii"; ?>Setaria italica"; ?>Solanum lycopersicum"; ?>Solanum tuberosum"; ?>Sorghum bicolor"; ?>Vitis vinifera"; ?>Zea mays"; ?>

Gene Ontology

GO:0005634	; C:nucleus
GO:0005524	; F:ATP binding
GO:0046872	; F:metal ion binding
GO:0004715	; F:non-membrane spanning protein tyrosine kinase activity
GO:0004672	; F:protein kinase activity
GO:0004674	; F:protein serine/threonine kinase activity
GO:0007050	; P:cell cycle arrest
GO:0051301	; P:cell division
GO:0000076	; P:DNA replication checkpoint
GO:0007067	; P:mitosis

KEGG

ath:AT1G02970

;

InterPros

IPR011009	; Kinase-like_dom.
IPR000719	; Prot_kinase_cat_dom.
IPR017441	; Protein_kinase_ATP_BS.
IPR008271	; Ser/Thr_kinase_AS.

Pfam

PF00069

; Pkinase; 1.

SMARTs

Prosites

PS00107	; PROTEIN_KINASE_ATP; 1.
PS50011	; PROTEIN_KINASE_DOM; 1.
PS00108	; PROTEIN_KINASE_ST; 1.

Prints

Created Date

20-Feb-2013