EPS-ART-00084

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EPS-ART-00084

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
PPM/PP2C	135.6	4.5E-39	125	395	271

Status

Reviewed

Ensembl Protein

AT2G20050.1

UniProt Accession

Q9SL76; B5KQ16; B5KQ17; Q0WLT6; Q9SL77;

Protein Name

Probable inactive cyclic nucleotide-dependent protein kinase At2g20050

Protein Synonyms/Alias

AtPP2C19;

Gene Name

AT2G20050

Gene Synonyms/Alias

At2g20050; T2G17.15;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
AT2G20050	AT2G20050.1	AT2G20050.1
AT2G20050	AT2G20050.2	AT2G20050.2

Organism

Arabidopsis thaliana

Functional Description

Protein Length

1094

Protein Sequence
(FASTA)

MGCAYSKTCI GQICATKENS IRQTHQQAPS RGGTRATAAA AAVEEDNPVF NFSSDAVDDV 60

DNDEIHQLGL SRDQEWGITR LSRVSSQFLP PDGSRVVKVP SCNYELRCSF LSQRGYYPDA 120

LDKANQDSFA IHTPFGSNSD DHFFGVFDGH GEFGAQCSQF VKRRLCENLL RHGRFRVDPA 180

EACNSAFLTT NSQLHADLVD DSMSGTTAIT VMVRGRTIYV ANAGDSRAVL AEKRDGDLVA 240

VDLSIDQTPF RPDELERVKL CGARVLTLDQ IEGLKNPDVQ CWGTEEDDDG DPPRLWVPNG 300

MYPGTAFTRS IGDSIAETIG VVANPEIAVV ELTPDNPFFV VASDGVFEFI SSQTVVDMVA 360

KHKDPRDACA AIVAESYRLW LQYETRTDDI TIIVVHIDGL KDDAPRQLSS TGTQLQPPIP 420

QVVELTGSES PSTFGWNSKN QRVRHDLSRA RIRAIENSLE NGHAWVPPSP AHRKTWEEEA 480

HIERVLRDHF LFRKLTDSQC QVLLDCMQRL EANPGDIVVK QGGEGDCFYV VGSGEFEVLA 540

TQDGKNGEVP RILQRYTAEK QSSFGELALM HNKPLQASVR AVDHGTLWAL KREDFRGILM 600

SEFSNLASLK LLRSVDLLSR LTILQLSHVA ESLSEACFSD GQTIVTKDQK LQGLYVIQKG 660

RVKISFCTEV LESQNVSSLT TGITNEYDNL EIGTEVSIEK HEGSYFGEWA LLGELKDSLS 720

VVAVGEVVCV VLTKENFESA VGPLTNISDD GPKTRHSSFE LSKESAKVTD TTALAKATLA 780

DLEWTTCLST TDCSEIGLVH LKDKENLLSL KRFSKQKVKK LGKEAQVLKE RNLMKNVIKP 840

SAIVPEILCT CVDQTFAAIL LNTTLACPIS SLLHSPLDES SVRFITGSLV SAIEDIHKNE 900

ILFRGSSPEL LMLDQSGYLQ IVDFRFAKKL SGERTFTICG NADYLAPEIV QGKGHGYAAD 960

WWALGVLIYY MLEGEMPFGS WRESELDTFQ KIAKGQLTFP RVLSSEAEDL ITKLLEVDEN 1020

LRFGSQGGPE SIKKHPWFNG LKWEAISNRE FQVPQEIISR IHHHLENDNV LPLETSKSLD 1080

TTEDQDAQNW LEEW 1094

Nucleotide Sequence
(FASTA)

ATGGGTTGTG CTTATTCCAA AACTTGCATT GGTCAGATTT GTGCTACGAA AGAGAATAGT 60

ATCAGGCAAA CGCATCAACA AGCTCCGTCC AGAGGTGGCA CGAGAGCTAC TGCGGCGGCG 120

GCGGCGGTTG AGGAGGATAA TCCGGTGTTT AATTTCTCTT CAGATGCGGT GGATGATGTA 180

GATAACGACG AAATTCATCA GCTTGGTTTG AGTAGAGACC AGGAATGGGG GATTACTCGG 240

CTTTCTAGGG TTTCTTCTCA ATTTCTACCA CCAGATGGGT CAAGGGTTGT CAAGGTTCCG 300

TCTTGTAACT ATGAATTGAG ATGCTCGTTT TTGTCTCAAA GAGGGTATTA TCCTGATGCT 360

CTTGATAAGG CCAATCAAGA TAGTTTCGCT ATTCATACTC CTTTTGGGAG TAATTCGGAT 420

GATCATTTCT TTGGGGTTTT TGATGGGCAT GGTGAGTTTG GTGCTCAGTG TTCTCAGTTT 480

GTGAAGCGGA GGTTGTGTGA GAATCTTCTT AGACATGGTA GGTTTCGTGT GGATCCTGCT 540

GAGGCTTGTA ATTCGGCTTT CTTGACCACG AATTCTCAGT TACATGCTGA TTTGGTAGAT 600

GATAGTATGA GTGGGACTAC TGCGATTACG GTTATGGTTA GAGGAAGGAC GATATATGTG 660

GCGAATGCAG GTGATTCCAG GGCGGTTTTA GCTGAGAAGA GGGATGGTGA TCTTGTAGCT 720

GTTGATTTGT CTATTGACCA GACTCCTTTT AGACCTGATG AGCTTGAAAG GGTTAAGCTT 780

TGTGGTGCTA GAGTTCTTAC TCTTGATCAG ATTGAAGGCT TGAAAAATCC TGATGTTCAG 840

TGTTGGGGTA CTGAGGAAGA TGATGATGGA GACCCTCCGA GACTTTGGGT GCCTAATGGA 900

ATGTATCCTG GAACTGCTTT TACCAGGAGT ATTGGTGATT CTATTGCAGA GACAATTGGT 960

GTTGTTGCTA ACCCTGAGAT TGCTGTTGTT GAGCTCACAC CGGATAATCC TTTCTTTGTG 1020

GTTGCTAGTG ATGGCGTATT TGAGTTCATC TCTAGTCAAA CGGTGGTTGA CATGGTTGCA 1080

AAACATAAGG ATCCTCGAGA TGCTTGTGCT GCAATTGTTG CTGAATCATA TAGGCTATGG 1140

CTACAGTATG AAACTCGGAC AGATGATATA ACAATCATCG TCGTGCACAT TGATGGACTA 1200

AAAGATGATG CTCCTCGGCA ATTGTCGAGT ACAGGAACTC AATTGCAGCC TCCTATTCCA 1260

CAAGTTGTGG AGCTTACAGG TTCGGAATCT CCTTCCACCT TTGGATGGAA CTCTAAAAAT 1320

CAACGTGTGA GGCATGATCT ATCTCGAGCT AGGATACGTG CCATTGAGAA TTCACTAGAG 1380

AATGGACATG CTTGGGTTCC TCCATCTCCA GCCCACAGAA AAACCTGGGA AGAAGAAGCA 1440

CACATTGAGC GGGTGTTGCG TGACCATTTC CTCTTCAGGA AACTCACTGA TTCTCAGTGC 1500

CAGGTTTTGT TGGACTGCAT GCAAAGGCTT GAAGCTAATC CAGGGGATAT TGTTGTGAAA 1560

CAGGGTGGTG AAGGGGACTG CTTCTACGTT GTGGGTAGTG GAGAATTCGA GGTCTTAGCA 1620

ACTCAGGATG GAAAGAACGG TGAGGTACCT AGGATCTTGC AGCGTTATAC AGCTGAGAAA 1680

CAATCATCAT TCGGTGAACT CGCCTTAATG CATAACAAGC CGCTTCAGGC TTCTGTACGT 1740

GCTGTAGATC ACGGAACATT GTGGGCCTTA AAAAGAGAAG ATTTTCGAGG AATTTTGATG 1800

TCTGAGTTTT CAAACTTAGC ATCCTTGAAG CTTCTTCGTT CTGTCGATCT TCTTTCCCGT 1860

CTTACAATTT TGCAACTAAG CCATGTTGCA GAGTCTCTTT CCGAAGCTTG CTTTTCTGAT 1920

GGACAAACAA TTGTTACCAA GGACCAAAAA CTTCAGGGCC TGTATGTTAT CCAGAAGGGA 1980

CGCGTAAAAA TATCTTTCTG TACAGAGGTG TTGGAGAGTC AAAATGTTTC AAGCCTTACA 2040

ACTGGAATCA CTAACGAGTA TGACAATCTT GAAATCGGAA CAGAAGTCTC CATAGAAAAG 2100

CATGAAGGAA GTTACTTTGG TGAATGGGCT CTTCTTGGCG AACTCAAAGA TTCCTTAAGT 2160

GTGGTTGCAG TTGGCGAAGT GGTTTGTGTG GTTTTAACAA AAGAAAATTT CGAGTCAGCG 2220

GTCGGCCCTC TGACCAATAT TTCAGATGAC GGTCCCAAGA CAAGACATTC TTCATTCGAG 2280

CTGTCAAAGG AATCTGCAAA AGTTACCGAT ACCACAGCTC TTGCTAAAGC CACTCTTGCA 2340

GACCTGGAAT GGACAACATG CTTGAGTACA ACAGACTGTA GTGAGATTGG GCTCGTGCAT 2400

TTGAAAGATA AAGAAAATTT GCTCAGCTTG AAAAGATTTT CAAAGCAAAA GGTGAAAAAG 2460

TTAGGTAAAG AGGCACAAGT ATTGAAAGAG CGGAATCTGA TGAAGAACGT AATAAAGCCC 2520

TCAGCTATTG TTCCCGAAAT CTTGTGTACT TGTGTCGATC AAACATTTGC GGCAATCTTA 2580

CTGAACACTA CTCTTGCCTG TCCTATCTCT TCTCTGCTTC ACTCTCCGCT TGACGAGTCA 2640

TCTGTCCGTT TCATTACCGG CTCACTTGTG TCTGCCATAG AAGACATACA CAAGAACGAG 2700

ATTCTCTTCA GAGGTTCATC CCCCGAGTTA CTGATGTTGG ATCAATCCGG ATATCTACAG 2760

ATTGTAGACT TCAGATTCGC CAAGAAATTG TCCGGGGAAC GAACATTTAC AATCTGTGGA 2820

AATGCAGATT ATTTAGCCCC AGAAATTGTT CAAGGCAAAG GCCATGGTTA TGCAGCTGAC 2880

TGGTGGGCTC TTGGAGTTTT GATCTACTAT ATGCTAGAAG GAGAAATGCC ATTCGGGTCA 2940

TGGAGAGAGA GCGAGCTCGA CACGTTTCAG AAGATTGCAA AAGGCCAACT AACTTTCCCT 3000

CGAGTTCTAA GCTCAGAAGC TGAAGATCTC ATCACAAAGT TGCTTGAAGT TGATGAAAAC 3060

CTGCGGTTTG GTAGCCAAGG AGGTCCAGAA TCAATCAAGA AACATCCCTG GTTCAATGGT 3120

CTTAAATGGG AAGCTATTAG TAACCGCGAA TTTCAAGTTC CTCAAGAGAT AATCTCGCGA 3180

ATTCATCACC ATTTGGAGAA TGATAACGTT CTCCCTCTAG AAACTTCTAA ATCGCTTGAC 3240

ACAACAGAAG ATCAAGATGC TCAAAACTGG CTTGAAGAAT GGTAA 3285

Domain Profile

S: 15    medavtsspnflkspekkdrlaffgvyDGhggsevakfcseklheilaeelesseseerk  74

          +d++++++ f+++++     +ffgv+DGhg  e   +cs+ +++ l e+l  +++ + +

Q: 125   NQDSFAIHTPFGSNSDD----HFFGVFDGHG--EFGAQCSQFVKRRLCENLLRHGRFRVD  178

         57888899999998775....8********9..888889999988888888888889999

S: 75    vkkalakaFlstdeelkvleeksldidksGstavvalikdsklyvanvGdSravLcrngk  134

           +a ++aFl+t ++l   ++   d + sG+ta ++++++ ++yvan+GdSravL+ +++

Q: 179   PAEACNSAFLTTNSQL---HADLVDDSMSGTTAITVMVRGRTIYVANAGDSRAVLAEKRD  235

         999*************...666668888*****************************886

S: 135   ....avelssdHkpsredEleRieeaggsvilkdgaRv......................  168

             av ls d +p r+dEleR++  g++v   d   +

Q: 236   gdlvAVDLSIDQTPFRPDELERVKLCGARVLTLD--QIeglknpdvqcwgteedddgdpp  293

         6899***************************999..7788899*****************

S: 169   ........lGvLavsRalGdlylkpy.vtpePevtshrltsedefliLasDGlwdvvsne  219

                  + +a +R++Gd   + + v+++Pe+ +++lt+++ f++ asDG+++ +s++

Q: 294   rlwvpngmYPGTAFTRSIGDSIAETIgVVANPEIAVVELTPDNPFFVVASDGVFEFISSQ  353

         *****9997779************777*********************************

S: 220   eavslvrdvlekkeaeqeaakkLvklalargsk.dnvsaivv  260

         ++v++v ++ +  +a+ ++  +  +l l+++++ d++++ivv

Q: 354   TVVDMVAKHKDPRDACAAIVAESYRLWLQYETRtDDITIIVV  395

         ******999995555555544444454554443588888886

Domain Sequence
(FASTA)

NQDSFAIHTP FGSNSDDHFF GVFDGHGEFG AQCSQFVKRR LCENLLRHGR FRVDPAEACN 60

SAFLTTNSQL HADLVDDSMS GTTAITVMVR GRTIYVANAG DSRAVLAEKR DGDLVAVDLS 120

IDQTPFRPDE LERVKLCGAR VLTLDQIEGL KNPDVQCWGT EEDDDGDPPR LWVPNGMYPG 180

TAFTRSIGDS IAETIGVVAN PEIAVVELTP DNPFFVVASD GVFEFISSQT VVDMVAKHKD 240

PRDACAAIVA ESYRLWLQYE TRTDDITIIV V 271

Keyword

Alternative splicing; ATP-binding; Complete proteome; Hydrolase; Kinase; Magnesium; Manganese; Metal-binding; Nucleotide-binding; Phosphoprotein; Protein phosphatase; Reference proteome; Repeat; Serine/threonine-protein kinase; Transferase.

Sequence Source

Ensembl

Orthology

Ortholog group

Arabidopsis lyrata"; ?>Brachypodium distachyon"; ?>Brassica rapa"; ?>Chlamydomonas reinhardtii"; ?>Cyanidioschyzon merolae"; ?>Glycine max"; ?>Hordeum vulgare"; ?>Musa acuminata"; ?>Oryza brachyantha"; ?>Oryza glaberrima"; ?>Oryza indica"; ?>Oryza sativa"; ?>Physcomitrella patens"; ?>Populus trichocarpa"; ?>Selaginella moellendorffii"; ?>Setaria italica"; ?>Solanum lycopersicum"; ?>Sorghum bicolor"; ?>Vitis vinifera"; ?>Zea mays"; ?>

Gene Ontology

GO:0005952	; C:cAMP-dependent protein kinase complex
GO:0005886	; C:plasma membrane
GO:0005524	; F:ATP binding
GO:0008603	; F:cAMP-dependent protein kinase regulator activity
GO:0046872	; F:metal ion binding
GO:0004674	; F:protein serine/threonine kinase activity
GO:0004722	; F:protein serine/threonine phosphatase activity
GO:0006470	; P:protein dephosphorylation

KEGG

ath:AT2G20050

;

InterPros

IPR002373	; cAMP/cGMP_kin.
IPR018490	; cNMP-bd-like.
IPR000595	; cNMP-bd_dom.
IPR011009	; Kinase-like_dom.
IPR001932	; PP2C-like.
IPR000222	; PP2C_Mn2_Asp60_BS.
IPR000719	; Prot_kinase_cat_dom.
IPR015655	; Protein_Pase_2C.
IPR014710	; RmlC-like_jellyroll.

Pfam

PF00027	; cNMP_binding; 2.
PF00069	; Pkinase; 1.
PF00481	; PP2C; 1.

SMARTs

SM00100	; cNMP; 2.
SM00331	; PP2C_SIG; 1.
SM00332	; PP2Cc; 1.

Prosites

PS00888	; CNMP_BINDING_1; FALSE_NEG.
PS00889	; CNMP_BINDING_2; FALSE_NEG.
PS50042	; CNMP_BINDING_3; 2.
PS01032	; PP2C; 1.
PS00107	; PROTEIN_KINASE_ATP; FALSE_NEG.
PS50011	; PROTEIN_KINASE_DOM; 1.
PS00108	; PROTEIN_KINASE_ST; FALSE_NEG.

Prints

PR00103

; CAMPKINASE.

Created Date

20-Feb-2013