EKS-ART-00300

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EKS-ART-00300

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
TKL/IRAK	210.3	6.7E-62	481	732	252

Status

Reviewed

Ensembl Protein

AT2G45910.1

UniProt Accession

Q8GUH1; O80828;

Protein Name

Serine/threonine-protein kinase

Protein Synonyms/Alias

Plant U-box protein 33;

Gene Name

PUB33

Gene Synonyms/Alias

PUB33; At2g45910; F4I18.11;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
AT2G45910	AT2G45910.1	AT2G45910.1

Organism

Arabidopsis thaliana

Functional Description

Functions as an E3 ubiquitin ligase (By similarity).

Protein Length

834

Protein Sequence
(FASTA)

MALVSPIPAM GERAGSMRFH GIGSPGSRSS RSGIMDEPVS RLIDEKIFVA VDKHVAKSKS 60

TLIWALQNTG GKKICLIHVH QPSQMIPLMG AKFPVGAVKE EEVRVFREKE REKVHMILDD 120

YLRICQQRGV RAEKMFIEME SIENGIVQLI SELGIRKLVM GAAADRHYSR RMTDLKSRKA 180

IFVRREAPTL CQIWFTCKGY LIHTREATMD DTESEYASPR PSISASDLLQ TFSTPESEHQ 240

HISRVQSTDS VQQLVSNGSS TEQSGRVSDG SLNTDEEERE SDGSEVTGSA TVMSSGHSSP 300

SSFPDGVDDS FNVKIRKATS EAHSSKQEAF AETLRRQKAE KNALDAIRRA KQSESAYSEE 360

LKRRKDTEIA VAKEKERFIT IKNEQEVIME ELQSAMAQKA MLESQIAKSD GTMEKLNQKL 420

DIAVKLLQKL RDEREELQTE RDRALREAEE LRSHAETSTL QLPQYFTDFS FSEIEEATNH 480

FDSTLKIGEG GYGSIYVGLL RHTQVAIKML NPNSSQGPVE YQQEVDVLSK MRHPNIITLI 540

GACPEGWSLV YEYLPGGSLE DRLTCKDNSP PLSWQNRVRI ATEICAALVF LHSNKAHSLV 600

HGDLKPANIL LDSNLVSKLS DFGTCSLLHP NGSKSVRTDV TGTVAYLDPE ASSSGELTPK 660

SDVYSFGIIL LRLLTGRPAL RISNEVKYAL DNGTLNDLLD PLAGDWPFVQ AEQLARLALR 720

CCETVSENRP DLGTEVWRVL EPMRASSGGS SSFHLGRNEH RIAPPYFICP IFQEVMQDPH 780

VAADGFTYEA EAIRAWLDSE HDTSPMTNVK LSHTSLIANH ALRSAIQEWL QHHL 834

Nucleotide Sequence
(FASTA)

ATGGCTTTGG TGAGTCCAAT TCCAGCAATG GGTGAAAGAG CTGGATCCAT GAGATTTCAC 60

GGAATTGGTA GCCCAGGATC AAGGTCTAGT AGGAGCGGGA TTATGGACGA ACCTGTCTCG 120

CGGCTGATTG ACGAGAAGAT CTTCGTTGCA GTGGATAAAC ACGTGGCCAA GAGCAAATCT 180

ACACTCATTT GGGCTTTGCA AAACACTGGA GGCAAGAAGA TTTGCCTTAT TCATGTTCAT 240

CAGCCCTCCC AAATGATCCC ACTTATGGGC GCCAAATTTC CAGTTGGTGC TGTGAAGGAG 300

GAAGAAGTCA GGGTTTTCAG GGAAAAGGAA AGGGAGAAAG TACATATGAT CCTAGACGAC 360

TACCTTCGTA TATGCCAGCA GAGAGGGGTC AGGGCAGAAA AGATGTTTAT TGAAATGGAA 420

TCCATCGAGA ATGGAATTGT GCAGCTCATC TCGGAGCTGG GAATCAGGAA ACTTGTCATG 480

GGAGCAGCAG CAGATAGACA CTATTCAAGG AGAATGACGG ATCTGAAGTC CAGGAAAGCC 540

ATCTTCGTAC GCCGAGAAGC ACCTACTCTT TGTCAAATAT GGTTTACTTG CAAAGGATAC 600

TTGATACATA CAAGGGAAGC TACTATGGAC GACACTGAAT CAGAATATGC ATCTCCGCGT 660

CCTTCCATAA GTGCAAGTGA TCTACTTCAA ACTTTCTCTA CACCGGAGTC TGAACATCAG 720

CATATATCTC GAGTACAGAG CACCGATTCA GTTCAACAAT TAGTAAGCAA TGGAAGCTCA 780

ACTGAACAAA GCGGGAGGGT GTCGGATGGG TCTTTAAATA CTGATGAGGA GGAGAGAGAA 840

TCTGATGGCA GTGAAGTAAC GGGAAGTGCA ACAGTTATGA GTTCTGGCCA TTCATCTCCC 900

TCCAGTTTTC CGGATGGGGT GGATGATTCT TTTAACGTTA AAATCCGAAA AGCCACGTCA 960

GAGGCTCACA GTTCAAAACA AGAAGCTTTT GCAGAGACTC TTAGGCGTCA GAAAGCCGAA 1020

AAAAACGCAC TTGATGCAAT CAGAAGGGCT AAACAATCAG AAAGTGCCTA TTCTGAGGAA 1080

TTGAAGCGAA GGAAAGATAC GGAGATAGCA GTAGCGAAAG AGAAAGAGAG ATTTATAACA 1140

ATAAAAAACG AGCAAGAGGT GATCATGGAA GAACTTCAAA GTGCAATGGC TCAGAAAGCG 1200

ATGCTTGAGA GCCAGATAGC AAAATCTGAT GGTACAATGG AAAAGCTAAA CCAGAAGCTT 1260

GATATAGCTG TGAAGTTGTT GCAAAAGTTG AGGGATGAAC GAGAGGAGTT GCAGACAGAA 1320

CGTGACAGAG CACTCAGAGA AGCTGAGGAG TTAAGGAGTC ACGCAGAAAC CTCAACCCTA 1380

CAGTTGCCTC AGTACTTCAC GGATTTCTCA TTCTCCGAGA TTGAGGAAGC AACTAATCAC 1440

TTTGATTCGA CCCTGAAGAT TGGGGAAGGT GGATATGGAA GCATCTACGT TGGTTTACTG 1500

CGTCATACTC AGGTGGCTAT AAAAATGTTG AATCCTAACA GTTCGCAAGG CCCTGTAGAA 1560

TATCAACAGG AGGTTGATGT CCTAAGTAAA ATGAGGCATC CAAATATCAT TACGCTAATT 1620

GGAGCATGTC CAGAGGGGTG GAGTCTTGTC TATGAGTATC TTCCGGGTGG AAGCCTTGAA 1680

GACAGGCTTA CTTGCAAAGA CAACTCTCCA CCCTTGTCGT GGCAAAATCG TGTGCGCATT 1740

GCCACTGAGA TATGTGCAGC ACTTGTCTTT CTTCATTCTA ATAAAGCTCA TAGCTTAGTA 1800

CATGGTGATT TGAAGCCGGC AAATATCCTA CTCGATTCCA ATCTCGTTAG CAAGTTAAGT 1860

GACTTTGGGA CCTGCTCACT ACTTCATCCT AACGGAAGCA AAAGTGTGAG AACTGACGTT 1920

ACAGGCACAG TTGCCTATTT GGATCCAGAA GCTTCCAGTA GTGGAGAGCT AACTCCTAAG 1980

TCAGATGTTT ACTCGTTTGG GATTATCTTA TTGCGCTTGT TGACCGGGAG ACCTGCCTTA 2040

CGGATATCAA ATGAAGTGAA ATATGCCCTC GATAATGGAA CATTGAACGA CCTCTTGGAC 2100

CCTTTAGCAG GGGACTGGCC CTTTGTTCAG GCTGAGCAGC TTGCTCGCTT AGCATTGAGA 2160

TGCTGCGAGA CTGTCAGCGA GAATCGTCCA GATCTTGGAA CTGAAGTGTG GAGGGTACTT 2220

GAACCTATGA GGGCTTCTAG TGGAGGATCT TCATCTTTCC ATCTTGGCCG TAACGAGCAC 2280

CGGATAGCCC CTCCATACTT CATTTGCCCC ATTTTCCAGG AAGTCATGCA GGATCCACAC 2340

GTGGCTGCAG ACGGTTTCAC ATACGAAGCA GAGGCTATAA GAGCGTGGCT GGACAGTGAG 2400

CATGATACTT CACCAATGAC CAATGTCAAG CTTTCTCACA CCAGCCTAAT AGCTAACCAT 2460

GCTCTTCGTT CTGCGATTCA AGAGTGGCTT CAACATCATT TGTGA 2505

Domain Profile

S: 1     fsednrigeGgfgevyrgelkntavavkklkeeadlslkelkqsfltElkvlarfrHdni  60

         f++  +igeGg+g +y g l+ t+va+k l+ + ++ + e    +++E+ vl+++rH+ni

Q: 481   FDSTLKIGEGGYGSIYVGLLRHTQVAIKMLNPNSSQGPVE----YQQEVDVLSKMRHPNI  536

         67789***************************99887765....78899***********

S: 61    lellgysaeseklcLvYqymknGsLedrLqcqkgsepLsWpqRlsillGtaraiefLHea  120

         + l+g + e+   +LvY+y+++GsLedrL+c+++s+pLsW+ R+ i++ +  a+ fLH++

Q: 537   ITLIGACPEG--WSLVYEYLPGGSLEDRLTCKDNSPPLSWQNRVRIATEICAALVFLHSN  594

         ******9876..89**********************************************

S: 121   sp.slihgdiksaNiLLDekltpKlgDfglar.fapesekqsstvlrtskvrgtlaYlpe  178

         +  sl+hgd+k+aNiLLD +l +Kl+Dfg +  ++p+  k+ +       v+gt+aYl +

Q: 595   KAhSLVHGDLKPANILLDSNLVSKLSDFGTCSlLHPNGSKSVR-----TDVTGTVAYLDP  649

         999****************************955665555443.....4579********

S: 179   efirvgqltvkvDvySfGiVllEvltGlravdedrktkyLkdllkeeieeekveilekfl  238

         e+  +g+lt k+DvySfGi+ll +ltG++a   +++ ky +d            +l++ l

Q: 650   EASSSGELTPKSDVYSFGIILLRLLTGRPALRISNEVKYALDN----------GTLNDLL  699

         ******************************9999999988876..........478999*

S: 239   dkkagkleeellealielalaclaekakkrPtm  271

         d  ag+ +   +e+l++lal+c ++  ++rP++

Q: 700   DPLAGDWPFVQAEQLARLALRCCETVSENRPDL  732

         *******************************98

Domain Sequence
(FASTA)

FDSTLKIGEG GYGSIYVGLL RHTQVAIKML NPNSSQGPVE YQQEVDVLSK MRHPNIITLI 60

GACPEGWSLV YEYLPGGSLE DRLTCKDNSP PLSWQNRVRI ATEICAALVF LHSNKAHSLV 120

HGDLKPANIL LDSNLVSKLS DFGTCSLLHP NGSKSVRTDV TGTVAYLDPE ASSSGELTPK 180

SDVYSFGIIL LRLLTGRPAL RISNEVKYAL DNGTLNDLLD PLAGDWPFVQ AEQLARLALR 240

CCETVSENRP DL 252

Keyword

ATP-binding; Coiled coil; Complete proteome; Kinase; Ligase; Nucleotide-binding; Reference proteome; Serine/threonine-protein kinase; Transferase; Ubl conjugation pathway.

Sequence Source

Ensembl

Orthology

Ortholog group

Arabidopsis lyrata"; ?>Brachypodium distachyon"; ?>Brassica rapa"; ?>Glycine max"; ?>Hordeum vulgare"; ?>Musa acuminata"; ?>Oryza brachyantha"; ?>Oryza glaberrima"; ?>Oryza indica"; ?>Oryza sativa"; ?>Populus trichocarpa"; ?>Setaria italica"; ?>Solanum lycopersicum"; ?>Solanum tuberosum"; ?>Sorghum bicolor"; ?>Vitis vinifera"; ?>Zea mays"; ?>

Gene Ontology

GO:0000151	; C:ubiquitin ligase complex
GO:0005524	; F:ATP binding
GO:0004674	; F:protein serine/threonine kinase activity
GO:0004842	; F:ubiquitin-protein ligase activity
GO:0006950	; P:response to stress

KEGG

ath:AT2G45910

;

InterPros

IPR011009	; Kinase-like_dom.
IPR000719	; Prot_kinase_cat_dom.
IPR017441	; Protein_kinase_ATP_BS.
IPR014729	; Rossmann-like_a/b/a_fold.
IPR008271	; Ser/Thr_kinase_AS.
IPR003613	; Ubox_domain.
IPR006016	; UspA.
IPR013083	; Znf_RING/FYVE/PHD.

Pfam

PF00069	; Pkinase; 1.
PF04564	; U-box; 1.
PF00582	; Usp; 1.

SMARTs

SM00504

; Ubox; 1.

Prosites

PS00107	; PROTEIN_KINASE_ATP; 1.
PS50011	; PROTEIN_KINASE_DOM; 1.
PS00108	; PROTEIN_KINASE_ST; 1.

Prints

Created Date

20-Feb-2013