EKS-ART-00454

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EKS-ART-00454

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
CAMK/CAMKL	352.6	1.8E-105	19	271	253

Status

Reviewed

Ensembl Protein

AT3G01090.1

UniProt Accession

Q38997; A6XGR0; O04728; Q38987; Q39076; Q8RWD2;

Protein Name

SNF1-related protein kinase catalytic subunit alpha KIN10

Protein Synonyms/Alias

AKIN10; AKIN alpha-2; AKINalpha2;

Gene Name

KIN10

Gene Synonyms/Alias

KIN10; AK21, SKIN10, SNR2; At3g01090; T4P13.22;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
AT3G01090	AT3G01090.3	AT3G01090.3
AT3G01090	AT3G01090.2	AT3G01090.2
AT3G01090	AT3G01090.1	AT3G01090.1

Organism

Arabidopsis thaliana

Functional Description

Catalytic subunit of the probable trimeric SNF1-relatedprotein kinase (SnRK) complex, which may play a role in a signal transduction cascade regulating gene expression and carbohydrate metabolism in higher plants. The SnRK complex may also be involved in the regulation of fatty acid synthesis by phosphorylation of acetyl-CoA carboxylase and in assimilation of nitrogen by phosphorylating nitrate reductase. In vitro, KIN10 exhibits kinase activity on sucrose phosphate synthase and the kinase activity is inhibited by PRL1. May be a subunit of a SCF ubiquitin ligase complex and thus be involved in proteasomal ubiquitination.

Protein Length

512

Protein Sequence
(FASTA)

MDGSGTGSRS GVESILPNYK LGRTLGIGSF GRVKIAEHAL TGHKVAIKIL NRRKIKNMEM 60

EEKVRREIKI LRLFMHPHII RLYEVIETPT DIYLVMEYVN SGELFDYIVE KGRLQEDEAR 120

NFFQQIISGV EYCHRNMVVH RDLKPENLLL DSKCNVKIAD FGLSNIMRDG HFLKTSCGSP 180

NYAAPEVISG KLYAGPEVDV WSCGVILYAL LCGTLPFDDE NIPNLFKKIK GGIYTLPSHL 240

SPGARDLIPR MLVVDPMKRV TIPEIRQHPW FQAHLPRYLA VPPPDTVQQA KKIDEEILQE 300

VINMGFDRNH LIESLRNRTQ NDGTVTYYLI LDNRFRASSG YLGAEFQETM EGTPRMHPAE 360

SVASPVSHRL PGLMEYQGVG LRSQYPVERK WALGLQSRAH PREIMTEVLK ALQDLNVCWK 420

KIGHYNMKCR WVPNSSADGM LSNSMHDNNY FGDESSIIEN EAAVKSPNVV KFEIQLYKTR 480

DDKYLLDLQR VQGPQFLFLD LCAAFLAQLR VL 512

Nucleotide Sequence
(FASTA)

ATGGATGGAT CAGGCACAGG CAGTAGAAGT GGGGTAGAAT CGATTCTACC AAATTACAAG 60

CTTGGGAGAA CTCTTGGTAT TGGTTCCTTT GGTAGGGTGA AGATAGCTGA GCATGCATTG 120

ACAGGACATA AGGTTGCTAT CAAGATCCTC AATCGTCGCA AAATCAAGAA CATGGAGATG 180

GAGGAGAAAG TGAGGAGAGA GATCAAAATC TTGAGACTAT TTATGCATCC TCACATCATC 240

CGTCTCTATG AGGTTATAGA GACTCCCACA GATATTTATC TTGTCATGGA GTATGTGAAC 300

TCTGGTGAGC TATTTGACTA TATTGTTGAG AAGGGTAGAT TGCAGGAGGA TGAGGCGAGG 360

AACTTTTTTC AGCAGATAAT ATCAGGAGTG GAATACTGCC ATCGAAACAT GGTGGTTCAC 420

AGAGACCTCA AGCCTGAAAA CTTGCTTTTG GACTCTAAAT GCAATGTAAA GATTGCTGAT 480

TTTGGCCTGA GCAACATAAT GCGAGATGGT CATTTTTTGA AGACAAGTTG TGGAAGTCCA 540

AATTATGCCG CTCCAGAGGT AATTTCGGGC AAGTTATATG CTGGCCCTGA AGTAGATGTC 600

TGGAGCTGTG GTGTGATACT CTACGCTCTT CTCTGTGGGA CGCTTCCATT TGATGATGAA 660

AACATTCCCA ACCTTTTTAA GAAGATAAAG GGAGGGATAT ACACATTACC TAGCCATTTA 720

TCTCCTGGTG CTAGAGATTT GATCCCCCGG ATGCTTGTAG TTGACCCCAT GAAACGAGTA 780

ACCATCCCTG AGATCCGGCA ACACCCTTGG TTCCAAGCTC ATCTTCCGAG GTATTTAGCT 840

GTTCCTCCTC CAGATACTGT GCAACAGGCA AAAAAGATTG ACGAGGAGAT TCTCCAAGAA 900

GTTATCAATA TGGGATTTGA CAGAAACCAC CTCATCGAAT CGCTCCGCAA CCGAACCCAG 960

AATGATGGCA CTGTGACGTA CTATCTGATA CTGGACAATC GTTTCCGTGC CTCTAGTGGT 1020

TATCTCGGGG CTGAGTTTCA AGAGACCATG GAAGGTACTC CCCGTATGCA TCCAGCAGAA 1080

AGCGTTGCTT CACCTGTTAG CCATCGGCTT CCAGGACTGA TGGAATATCA AGGAGTTGGC 1140

TTGAGATCTC AATACCCTGT TGAGAGAAAA TGGGCTCTTG GACTTCAGTC TCGGGCTCAT 1200

CCCCGTGAAA TAATGACGGA AGTCCTGAAA GCCCTGCAAG ATTTGAATGT ATGTTGGAAG 1260

AAGATAGGGC ACTACAACAT GAAGTGCAGA TGGGTTCCTA ACAGCAGCGC AGATGGTATG 1320

CTCAGTAACT CGATGCACGA TAACAACTAC TTTGGAGACG AGTCCAGCAT AATAGAGAAC 1380

GAAGCAGCTG TTAAGTCGCC CAATGTTGTC AAGTTTGAAA TTCAGTTGTA TAAAACTCGG 1440

GACGACAAGT ATCTACTGGA TTTGCAGAGA GTACAAGGTC CTCAGTTCTT GTTCTTGGAT 1500

CTGTGTGCTG CTTTTCTTGC TCAGCTCCGA GTCCTCTGA 1539

Domain Profile

S: 1     ykllktlGegsfgkVklakhketkekvavKiikkkkl.seeslekikrEieilkklkhpn  59

         ykl++tlG gsfg+Vk a+h +t++kva+Ki++++k+ + e  ek++rEi+il+ + hp+

Q: 19    YKLGRTLGIGSFGRVKIAEHALTGHKVAIKILNRRKIkNMEMEEKVRREIKILRLFMHPH  78

         99*********************************994455566****************

S: 60    iikllevietkkklylvleyasggeLfdyieekgrlkekearklfkqlvsavkYlhskgi  119

         ii+l+eviet +++ylv+ey++ geLfdyi+ekgrl+e+ear++f+q++s+v+Y+h++ +

Q: 79    IIRLYEVIETPTDIYLVMEYVNSGELFDYIVEKGRLQEDEARNFFQQIISGVEYCHRNMV  138

         ************************************************************

S: 120   vHrDlKpeNllldkkenikiaDFGlanlfkegklletfcGsppYaaPellkgkkYegpkv  179

         vHrDlKpeNllld+k n+kiaDFGl+n++++g+ l+t+cGsp+YaaPe+++gk Y gp+v

Q: 139   VHRDLKPENLLLDSKCNVKIADFGLSNIMRDGHFLKTSCGSPNYAAPEVISGKLYAGPEV  198

         ************************************************************

S: 180   DvWslGvvLyalvtgklPfdeenlkellekilkgkleipkklskelesLlrklLvvdpek  239

         DvWs+Gv+Lyal++g+lPfd+en+ +l++ki+ g +++p++ls+ +++L+ ++Lvvdp k

Q: 199   DVWSCGVILYALLCGTLPFDDENIPNLFKKIKGGIYTLPSHLSPGARDLIPRMLVVDPMK  258

         ************************************************************

S: 240   Ratieeilkhewl  252

         R+ti ei++h+w+

Q: 259   RVTIPEIRQHPWF  271

         ************7

Domain Sequence
(FASTA)

YKLGRTLGIG SFGRVKIAEH ALTGHKVAIK ILNRRKIKNM EMEEKVRREI KILRLFMHPH 60

IIRLYEVIET PTDIYLVMEY VNSGELFDYI VEKGRLQEDE ARNFFQQIIS GVEYCHRNMV 120

VHRDLKPENL LLDSKCNVKI ADFGLSNIMR DGHFLKTSCG SPNYAAPEVI SGKLYAGPEV 180

DVWSCGVILY ALLCGTLPFD DENIPNLFKK IKGGIYTLPS HLSPGARDLI PRMLVVDPMK 240

RVTIPEIRQH PWF 253

Keyword

Alternative splicing; ATP-binding; Carbohydrate metabolism; Complete proteome; Fatty acid biosynthesis; Fatty acid metabolism; Isopeptide bond; Kinase; Lipid biosynthesis; Lipid metabolism; Nitrate assimilation; Nucleotide-binding; Phosphoprotein; Reference proteome; Serine/threonine-protein kinase; Transferase; Ubl conjugation; Ubl conjugation pathway.

Sequence Source

Ensembl

Orthology

Ortholog group

Arabidopsis lyrata"; ?>Brachypodium distachyon"; ?>Brassica rapa"; ?>Chlamydomonas reinhardtii"; ?>Cyanidioschyzon merolae"; ?>Glycine max"; ?>Hordeum vulgare"; ?>Musa acuminata"; ?>Oryza brachyantha"; ?>Oryza glaberrima"; ?>Oryza indica"; ?>Oryza sativa"; ?>Physcomitrella patens"; ?>Populus trichocarpa"; ?>Selaginella moellendorffii"; ?>Setaria italica"; ?>Solanum lycopersicum"; ?>Solanum tuberosum"; ?>Sorghum bicolor"; ?>Vitis vinifera"; ?>Zea mays"; ?>

Gene Ontology

GO:0000152	; C:nuclear ubiquitin ligase complex
GO:0005524	; F:ATP binding
GO:0004674	; F:protein serine/threonine kinase activity
GO:0009738	; P:abscisic acid mediated signaling pathway
GO:0005975	; P:carbohydrate metabolic process
GO:0009594	; P:detection of nutrient
GO:0003006	; P:developmental process involved in reproduction
GO:0006633	; P:fatty acid biosynthetic process
GO:0042128	; P:nitrate assimilation
GO:0010260	; P:organ senescence
GO:0080022	; P:primary root development
GO:0006950	; P:response to stress
GO:0010182	; P:sugar mediated signaling pathway
GO:0010050	; P:vegetative phase change

KEGG

ath:AT3G01090

;

InterPros

IPR001772	; Kinase-assoc_KA1.
IPR011009	; Kinase-like_dom.
IPR000719	; Prot_kinase_cat_dom.
IPR017441	; Protein_kinase_ATP_BS.
IPR002290	; Ser/Thr_dual-sp_kinase_dom.
IPR008271	; Ser/Thr_kinase_AS.
IPR000449	; UBA/transl_elong_EF1B_N.
IPR015940	; UBA/transl_elong_EF1B_N_euk.

Pfam

PF02149	; KA1; 1.
PF00069	; Pkinase; 1.
PF00627	; UBA; 1.

SMARTs

SM00220	; S_TKc; 1.
SM00165	; UBA; 1.

Prosites

PS50032	; KA1; 1.
PS00107	; PROTEIN_KINASE_ATP; 1.
PS50011	; PROTEIN_KINASE_DOM; 1.
PS00108	; PROTEIN_KINASE_ST; 1.
PS50030	; UBA; 1.

Prints

Created Date

20-Feb-2013