EPS-ART-00007

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EPS-ART-00007

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
DSP/aDSP	57.5	2.3E-15	293	424	132

Status

Reviewed

Ensembl Protein

AT3G01510.1

UniProt Accession

F4J117; Q8LCU3; Q9SSA3;

Protein Name

Phosphoglucan phosphatase LSF1, chloroplastic

Protein Synonyms/Alias

Phosphoglucan phosphatase like sex Four1; Protein LIKE SEX4 1;

Gene Name

LSF1

Gene Synonyms/Alias

LSF1; PTPKIS2; At3g01510; F4P13.6;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
AT3G01510	AT3G01510.1	AT3G01510.1

Organism

Arabidopsis thaliana

Functional Description

Starch granule-associated phosphoglucan phosphataseinvolved in the control of starch accumulation. Participates in the regulation of the initial steps of starch degradation at the granule surface. May release a different set of phosphate groups from those removed by DSP4.

Protein Length

591

Protein Sequence
(FASTA)

MAFLQQISGL GALERSCPSI MIGSSFRSGN GRVFDGRGIA YLGSREKFGF NRRRRVVLRV 60

VAMSSSSTPF KMNLNEYMVT LEKPLGIRFA LSADGKIFVH AIKKGSNAEK ARIIMVGDTL 120

KKASDSSGGT LVEIKDFGDT KKMLVEKTGS FSLVLERPFS PFPIQYLLHL SDLDLLYNRG 180

RVSFVTWNKN LLSSNLRASS QGSGNSGYAA FSSKFFTPQG WKLLNRQSNS FQSGTKKNIL 240

SPPISPLVSV FSEDVPGDGE WGYGNFPLEE YIKALDRSKG ELSYNHALGM RYSKITEQIY 300

VGSCIQTEED VENLSEAGIT AILNFQGGTE AQNWGIDSQS INDACQKSEV LMINYPIKDA 360

DSFDLRKKLP LCVGLLLRLL KKNHRVFVTC TTGFDRSSAC VIAYLHWMTD TSLHAAYSFV 420

TGLHACKPDR PAIAWATWDL IAMVDDGKHD GTPTHSVTFV WNGHEGEEVL LVGDFTGNWK 480

EPIKATHKGG PRFETEVRLT QGKYYYKYII NGDWRHSATS PTERDDRGNT NNIIVVGDVA 540

NVRPTIQQPR KDANIIKVIE RVLTESERFR LAKAARCIAF SVCPIRLCPK S 591

Nucleotide Sequence
(FASTA)

ATGGCGTTTC TTCAACAAAT CTCCGGTTTA GGAGCTCTCG AACGATCTTG TCCGTCAATT 60

ATGATCGGAT CTTCATTCCG GAGCGGTAAT GGCCGTGTAT TCGACGGAAG AGGAATTGCG 120

TACTTGGGTT CGAGGGAAAA GTTCGGATTC AACAGACGAA GAAGAGTCGT TCTTAGAGTT 180

GTTGCGATGT CGTCTTCTTC TACTCCGTTC AAAATGAATT TGAATGAGTA TATGGTCACT 240

CTCGAGAAGC CCCTCGGGAT TCGCTTCGCA CTTTCAGCAG ATGGCAAGAT CTTCGTCCAC 300

GCCATCAAAA AAGGGAGCAA TGCGGAGAAG GCGAGAATCA TCATGGTAGG AGATACACTC 360

AAGAAGGCTA GTGATTCTTC TGGTGGTACT CTTGTGGAAA TCAAGGATTT TGGTGATACC 420

AAGAAGATGC TGGTAGAGAA AACTGGATCC TTTAGCCTTG TTCTTGAAAG ACCATTCTCT 480

CCCTTTCCCA TTCAGTATCT GCTTCATTTG AGCGACCTTG ATTTGCTCTA TAACAGAGGG 540

CGTGTGTCTT TTGTTACTTG GAACAAAAAC CTCTTATCAT CGAATCTCAG AGCATCATCA 600

CAAGGCAGTG GTAACTCTGG CTATGCTGCG TTTTCCTCCA AATTCTTTAC TCCTCAGGGA 660

TGGAAACTTT TGAATAGACA GAGCAATTCC TTTCAGTCAG GGACTAAGAA GAACATTCTT 720

TCTCCACCTA TCAGTCCACT GGTCTCTGTC TTTTCAGAAG ATGTTCCTGG AGATGGGGAA 780

TGGGGTTATG GGAATTTCCC TTTAGAGGAA TACATCAAAG CCCTTGACCG TTCTAAAGGA 840

GAGCTTTCTT ATAACCATGC TCTTGGAATG CGCTACAGTA AGATTACAGA GCAAATATAT 900

GTTGGCTCTT GCATACAAAC AGAAGAGGAT GTGGAAAACT TGTCGGAAGC TGGAATTACT 960

GCCATACTAA ATTTCCAAGG TGGAACTGAA GCTCAAAACT GGGGGATCGA TTCACAAAGT 1020

ATCAATGATG CATGCCAGAA ATCTGAAGTC TTAATGATCA ACTACCCTAT AAAGGATGCA 1080

GATTCCTTTG ATCTTAGGAA GAAGCTACCA CTTTGTGTTG GGCTCTTACT ACGGTTACTG 1140

AAAAAGAACC ATCGTGTCTT TGTAACTTGT ACCACTGGTT TTGACCGGTC ATCCGCTTGT 1200

GTGATTGCTT ATCTGCACTG GATGACTGAT ACCTCTCTTC ATGCTGCTTA TAGTTTCGTT 1260

ACTGGATTGC ACGCTTGCAA ACCTGACAGA CCTGCAATTG CTTGGGCAAC ATGGGATCTT 1320

ATCGCTATGG TGGACGATGG CAAACATGAT GGGACTCCAA CACACTCTGT AACCTTTGTG 1380

TGGAATGGAC ATGAGGGGGA GGAGGTATTG TTGGTTGGGG ATTTCACAGG AAATTGGAAA 1440

GAACCGATAA AAGCAACGCA CAAAGGCGGT CCACGTTTTG AAACTGAAGT TAGACTTACA 1500

CAGGGAAAAT ACTACTACAA GTACATAATC AACGGTGATT GGAGGCATTC AGCAACTTCA 1560

CCAACGGAAA GAGATGACCG AGGAAACACG AACAATATAA TTGTAGTTGG TGATGTTGCT 1620

AACGTCAGGC CTACAATTCA ACAACCAAGA AAGGATGCAA ATATCATAAA GGTGATCGAG 1680

AGAGTGTTAA CAGAGAGCGA AAGATTTAGG CTGGCGAAAG CAGCTCGGTG TATCGCATTC 1740

TCAGTTTGTC CAATAAGACT ATGCCCAAAG TCGTAG 1776

Domain Profile

S: 3     teilpglylgdqa.taldrreLsklgitvvinaslsv......ikvqviPeylikkgity  55

         ++i+ ++y+g+ + t  d  +Ls++git ++n   ++      i  q+i +++ k+++ +

Q: 293   SKITEQIYVGSCIqTEEDVENLSEAGITAILNFQGGTeaqnwgIDSQSINDACQKSEVLM  352

         689*******97615678889**********97643211222266799************

S: 56    knipvdDsdvtdllqyfdeaaefideakekggkvlvHCaaGvsRSatlvlaYLmkvegls  115

          n p++D+d++dl + ++ ++ ++ +  +k+++v+v C+ G+ RS + v+aYL  +++ s

Q: 353   INYPIKDADSFDLRKKLPLCVGLLLRLLKKNHRVFVTCTTGFDRSSACVIAYLHWMTDTS  412

         ************************************************************

S: 116   lsdAieavkrkr  127

         l+ A++ v

Q: 413   LHAAYSFVTGLH  424

         *****9997554

Domain Sequence
(FASTA)

SKITEQIYVG SCIQTEEDVE NLSEAGITAI LNFQGGTEAQ NWGIDSQSIN DACQKSEVLM 60

INYPIKDADS FDLRKKLPLC VGLLLRLLKK NHRVFVTCTT GFDRSSACVI AYLHWMTDTS 120

LHAAYSFVTG LH 132

Keyword

Carbohydrate metabolism; Chloroplast; Complete proteome; Hydrolase; Plastid; Reference proteome; Transit peptide.

Sequence Source

Ensembl

Orthology

Ortholog group

Arabidopsis lyrata"; ?>Brachypodium distachyon"; ?>Glycine max"; ?>Hordeum vulgare"; ?>Musa acuminata"; ?>Oryza brachyantha"; ?>Oryza glaberrima"; ?>Oryza indica"; ?>Oryza sativa"; ?>Physcomitrella patens"; ?>Populus trichocarpa"; ?>Setaria italica"; ?>Solanum tuberosum"; ?>Sorghum bicolor"; ?>Vitis vinifera"; ?>Zea mays"; ?>

Gene Ontology

GO:0009569	; C:chloroplast starch grain
GO:0009570	; C:chloroplast stroma
GO:0019203	; F:carbohydrate phosphatase activity
GO:0008138	; F:protein tyrosine/serine/threonine phosphatase activity
GO:0006470	; P:protein dephosphorylation
GO:0005983	; P:starch catabolic process

KEGG

ath:AT3G01510

;

InterPros

IPR000340	; Dual-sp_phosphatase_cat-dom.
IPR020422	; Dual-sp_phosphatase_subgr_cat.
IPR001478	; PDZ.

Pfam

PF00782

; DSPc; 1.

SMARTs

Prosites

PS00383	; TYR_PHOSPHATASE_1; FALSE_NEG.
PS50056	; TYR_PHOSPHATASE_2; FALSE_NEG.
PS50054	; TYR_PHOSPHATASE_DUAL; 1.

Prints

Created Date

20-Feb-2013