EKS-ART-00940

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EKS-ART-00940

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
Other/PEK	258.6	1.2E-76	449	754	306

Status

Reviewed

Ensembl Protein

AT3G59410.2

UniProt Accession

Q9LX30; Q8H2D3;

Protein Name

Probable serine/threonine-protein kinase GCN2

Protein Synonyms/Alias

Gene Name

GCN2

Gene Synonyms/Alias

GCN2; At3g59410; F25L23_270;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
AT3G59410	AT3G59410.1	AT3G59410.1
AT3G59410	AT3G59410.2	AT3G59410.2

Organism

Arabidopsis thaliana

Functional Description

Protein kinase that may be involved in thephosphorylation of the alpha subunit of eIF-2 (By similarity).

Protein Length

1265

Protein Sequence
(FASTA)

MGRSSSKKKK KRGGSGRRGQ LKDHGSNADE DNELLSEEIT ALNCVNVGIM GFDRSDVTGK 60

NNSNYFSAIF QEDCKVVSDS RSPPQIAIKL RPYSKDMGYE DTDISAMLIV RCLPGYPYKC 120

PKLQITPEQG LTTADAEKLL SLLEDQANSN AREGRVMIFN LVEAAQEFLS EIIPESHDEE 180

SVPCLTAHRS TQFIEQPMLS NIAKSCSGGP FVYGFIDLFS GLEDARNWSL TPDENRGIVS 240

SVQSHPLDTS RILHQKPDKN LKRFEDHAKE EVALPAPIAK LNTVQEENVD DTSISSFDSS 300

KSTDDVESGL FQNEKKESNL QDDTAEDDST NSESESLGSW SSDSLAQDQV PQISKKDLLM 360

VHLLRVACTS RGPLADALPQ ITDELHELGI LSEEVLDLAS KSSPDFNRTF EHAFNQNMAS 420

TSVPQFWEPP SDSCEPNASL PSSRYLNDFE ELKPLGQGGF GHVVLCKNKL DGRQYAVKKI 480

RLKDKEIPVN SRIVREVATL SRLQHQHVVR YYQAWFETGV VDPFAGANWG SKTAGSSMFS 540

YSGAVSTEIP EQDNNLESTY LYIQMEYCPR TLRQVFESYN HFDKDFAWHL IRQIVEGLAH 600

IHGQGIIHRD FTPNNIFFDA RNDIKIGDFG LAKFLKLEQL DQDGGFSTDV AGSGVDSTGQ 660

AGTYFYTAPE IEQDWPKIDE KADMYSLGVV FFELWHPFGT AMERHVILTN LKLKGELPLK 720

WVNEFPEQAS LLRRLMSPSP SDRPSATELL KHAFPPRMES ELLDNILRIM QTSEDSSVYD 780

RVVSVIFDEE VLEMKSHQSS RSRLCADDSY IQYTEINTEL RDYVVEITKE VFRQHCAKHL 840

EVIPMRLLSD CPQFSRKTVK LLTNGGDMLE LCYELRLPFV HWISVNQKSS FKRYEISHVY 900

RRAIGHSPPN PCLQADFDIV GGTLSLTEAE VLKVIVDITT HIFHRGSCDI HLNHGDLLDA 960

IWSWAGIKAE HRRKVAELLS MMGSLRPQSS ERKLKWVFIR RQLLQELKLP EAVVNRLQTV 1020

ASRFCGDADQ ALPRLRGALR ADRPTRKALD ELSNLLTYLR VWRIEEHVHI DVLMPPTESY 1080

HRNLFFQVFL TKENSSGTSN DGVLLAVGGR YDWLVQEVCD REHKMNLPGA VGVSLALETI 1140

FQHLPMDLRP IRNEVSTSVL VCSRGGGGLL VQRMELVAEL WEKSIKAEFV PTPDPSLTEQ 1200

YEYANEHEIK CLVIITESGV AQNQIEFVKV RHLELKKEKV VGREELVKFL LDAMAVQFRN 1260

PSVWS 1265

Nucleotide Sequence
(FASTA)

ATGGGTCGCA GCAGTTCGAA GAAAAAGAAG AAACGAGGAG GTAGCGGAAG AAGGGGTCAG 60

CTGAAAGACC ATGGATCTAA TGCTGATGAA GATAATGAAC TTCTATCTGA GGAGATCACT 120

GCTCTAAATT GTGTAAATGT TGGGATTATG GGATTTGATC GTTCAGATGT TACAGGGAAA 180

AACAATAGCA ATTATTTCTC TGCAATATTT CAAGAGGACT GCAAAGTTGT TTCTGATTCG 240

CGTTCGCCTC CGCAAATAGC AATCAAGCTC AGGCCGTACT CAAAGGACAT GGGATATGAA 300

GACACCGACA TATCTGCTAT GCTTATAGTT AGGTGCTTAC CAGGATATCC TTACAAGTGC 360

CCCAAGCTTC AGATTACTCC AGAACAAGGG TTGACAACAG CTGATGCTGA GAAGCTTTTA 420

TCTCTTCTCG AGGACCAGGC AAATTCCAAT GCTCGTGAAG GTCGGGTTAT GATATTCAAC 480

CTGGTGGAGG CTGCTCAAGA GTTTTTATCA GAAATCATTC CGGAAAGTCA TGATGAGGAA 540

TCTGTTCCAT GCTTGACTGC ACATCGAAGC ACTCAGTTCA TTGAGCAACC TATGCTTTCA 600

AATATAGCAA AATCCTGTTC TGGTGGACCT TTTGTGTATG GTTTTATAGA CCTATTTAGT 660

GGCTTGGAAG ATGCAAGAAA TTGGAGTCTG ACTCCAGATG AAAATAGGGG AATCGTATCT 720

TCAGTACAAT CTCACCCACT AGACACTTCA AGAATTTTGC ATCAGAAGCC AGACAAGAAT 780

CTGAAGCGAT TTGAAGACCA TGCTAAAGAA GAAGTTGCAC TGCCTGCTCC CATTGCCAAA 840

CTGAATACTG TTCAAGAGGA GAATGTTGAT GATACAAGCA TCTCTTCTTT TGACTCAAGT 900

AAATCTACTG ACGATGTGGA ATCTGGATTA TTCCAAAATG AGAAGAAGGA ATCAAATCTT 960

CAAGATGATA CAGCTGAAGA TGACAGCACT AACTCCGAAA GTGAGTCGCT GGGGTCATGG 1020

TCTTCTGATT CCTTAGCTCA AGATCAAGTG CCTCAGATTA GCAAGAAAGA TCTGTTGATG 1080

GTCCATTTAC TTCGAGTAGC TTGCACTTCC CGAGGACCTT TGGCTGATGC ATTACCTCAG 1140

ATAACTGATG AACTGCATGA GCTTGGTATA TTGTCTGAAG AAGTGTTGGA TTTAGCTTCC 1200

AAATCATCTC CAGACTTTAA TAGAACCTTT GAACATGCAT TCAATCAAAA CATGGCCTCA 1260

ACCAGTGTTC CTCAGTTTTG GGAGCCACCT TCTGATTCTT GCGAGCCAAA TGCATCACTC 1320

CCAAGCTCGC GATATCTCAA TGATTTTGAA GAGTTGAAAC CCCTTGGCCA AGGTGGTTTC 1380

GGCCACGTTG TGTTGTGCAA AAATAAACTG GATGGAAGAC AATATGCAGT GAAGAAAATT 1440

CGACTGAAGG ACAAAGAGAT ACCTGTCAAC AGTCGGATAG TTCGAGAAGT AGCAACACTT 1500

TCCCGTTTGC AGCATCAGCA TGTTGTACGT TACTATCAGG CCTGGTTCGA AACAGGAGTT 1560

GTTGATCCCT TTGCTGGCGC AAATTGGGGA TCAAAAACTG CAGGGAGTTC AATGTTCAGC 1620

TACTCAGGTG CAGTGTCAAC TGAAATTCCT GAGCAGGACA ATAATCTTGA GTCGACTTAT 1680

CTATATATTC AAATGGAATA TTGTCCCAGG ACTCTCCGCC AGGTTTTTGA ATCATATAAC 1740

CACTTCGACA AAGACTTTGC ATGGCATTTA ATTCGCCAAA TTGTGGAAGG CTTAGCTCAT 1800

ATCCATGGAC AAGGAATAAT TCATCGGGAT TTTACACCTA ACAATATTTT CTTTGACGCT 1860

CGGAATGATA TTAAAATTGG GGATTTTGGT CTTGCAAAGT TCTTGAAGCT GGAACAGTTG 1920

GATCAAGATG GGGGTTTCTC TACGGATGTG GCTGGAAGCG GAGTCGATAG TACTGGTCAA 1980

GCTGGTACTT ACTTTTACAC AGCACCTGAA ATTGAGCAAG ATTGGCCTAA GATTGATGAA 2040

AAGGCCGACA TGTATAGCTT AGGGGTTGTG TTCTTTGAAC TTTGGCATCC TTTTGGAACC 2100

GCCATGGAGA GACACGTTAT TTTAACTAAC CTGAAGCTGA AAGGGGAGCT ACCTCTCAAA 2160

TGGGTAAATG AATTTCCCGA ACAGGCGTCT CTACTGCGGC GTTTGATGTC TCCAAGTCCA 2220

TCTGATCGTC CCTCTGCCAC AGAACTTCTT AAGCATGCAT TTCCTCCCAG AATGGAATCT 2280

GAGTTACTGG ACAATATTCT AAGAATAATG CAAACTTCTG AAGATTCAAG TGTTTATGAT 2340

AGAGTAGTAA GTGTGATATT TGATGAAGAA GTATTAGAGA TGAAAAGCCA TCAGTCTAGT 2400

AGATCGAGAC TCTGTGCAGA TGATAGTTAT ATTCAATACA CAGAGATAAA TACAGAGCTT 2460

CGTGATTATG TTGTTGAAAT AACAAAAGAA GTCTTTAGGC AGCATTGTGC GAAGCATCTA 2520

GAGGTCATAC CAATGCGCTT ACTTAGTGAT TGCCCCCAGT TTAGCAGGAA AACTGTAAAG 2580

CTTTTGACCA ATGGAGGAGA TATGCTTGAA CTATGCTATG AGCTACGACT GCCTTTTGTG 2640

CATTGGATAA GCGTAAATCA GAAATCCTCA TTCAAGCGAT ATGAAATATC TCATGTCTAC 2700

AGGAGAGCAA TTGGCCATTC TCCACCAAAT CCGTGTCTTC AGGCGGACTT TGACATTGTT 2760

GGAGGCACAC TATCCCTGAC AGAGGCAGAA GTTCTCAAGG TGATAGTAGA CATCACAACC 2820

CACATCTTTC ATCGCGGATC TTGTGACATT CATTTGAATC ATGGAGATTT GCTGGATGCG 2880

ATTTGGTCCT GGGCAGGAAT TAAGGCAGAG CATAGACGAA AGGTTGCAGA GCTTCTTTCC 2940

ATGATGGGAT CCTTGCGTCC TCAGTCATCT GAGCGGAAGC TAAAATGGGT TTTCATAAGG 3000

CGTCAACTTC TTCAGGAGTT GAAGTTACCT GAAGCTGTTG TCAATAGACT GCAGACTGTT 3060

GCTTCAAGGT TTTGTGGAGA TGCAGATCAA GCACTTCCTC GTTTAAGAGG GGCTCTGCGT 3120

GCTGATAGAC CTACCCGCAA AGCACTCGAT GAGTTGTCAA ACCTCTTAAC CTACCTGAGA 3180

GTCTGGAGGA TAGAAGAGCA TGTTCATATT GATGTTCTGA TGCCACCAAC TGAAAGTTAT 3240

CACCGGAATT TGTTTTTTCA GGTTTTCTTA ACCAAAGAAA ATAGCTCTGG GACATCTAAT 3300

GATGGCGTTT TACTTGCTGT TGGTGGTCGT TATGATTGGT TGGTGCAGGA AGTGTGTGAT 3360

CGTGAACATA AAATGAACCT CCCTGGTGCT GTTGGAGTTA GTCTTGCACT GGAGACAATA 3420

TTTCAGCATC TTCCTATGGA TCTAAGGCCT ATTAGAAATG AAGTCAGCAC CAGTGTACTT 3480

GTTTGTTCAA GAGGAGGTGG TGGTTTACTG GTCCAGCGCA TGGAACTAGT TGCGGAACTA 3540

TGGGAAAAAA GTATAAAGGC TGAGTTTGTT CCAACACCTG ATCCAAGTCT TACTGAGCAG 3600

TACGAATATG CAAATGAACA TGAAATCAAA TGTCTAGTGA TCATCACAGA GTCTGGAGTA 3660

GCTCAAAATC AAATAGAGTT TGTAAAGGTT CGTCACCTTG AACTGAAGAA GGAGAAAGTG 3720

GTAGGAAGAG AAGAACTTGT CAAATTTCTG CTGGATGCAA TGGCTGTTCA ATTTAGAAAC 3780

CCCTCTGTTT GGAGCTAA 3798

Domain Profile

S: 1     feeikvlGkGgfgvVlkaknkldkkeyaikriklnkakke.lekverevkaLaklehpni  59

         fee+k lG+Ggfg V+  knkld+++ya+k+i+l++++   ++++ rev +L++l+h+ +

Q: 449   FEELKPLGQGGFGHVVLCKNKLDGRQYAVKKIRLKDKEIPvNSRIVREVATLSRLQHQHV  508

         7999**********************************9999******************

S: 60    vrYfaawleekeesl............tdsseveaeaeleesesseesdtkllyiqmelc  107

         vrY++aw e+                 ++  + + ++++e  e++++ ++++lyiqme+c

Q: 509   VRYYQAWFETGVVDPfaganwgsktagSSMFSYSGAVSTEIPEQDNNLESTYLYIQMEYC  568

         ********99765446899999996653344555677888889999999***********

S: 108   eketLkdwiderkllesrselvmrlfkqllegleylHskgliHrdLkpsniFldsddqvK  167

           +tL+++ ++  +  +++  + +l++q++egl+++H +g+iHrd  p niF+d+ + +K

Q: 569   P-RTLRQVFESY-NHFDKDFAW-HLIRQIVEGLAHIHGQGIIHRDFTPNNIFFDARNDIK  625

         9.9*****9998.577888899.*************************************

S: 168   igDFgLatala.ealked.vkqdaqske.akhtgqvgtklymsPeql.eksqYnkkvDif  223

         igDFgLa+ l+ e+l +d  ++++ +++    tgq+gt  y +Pe      + ++k D++

Q: 626   IGDFGLAKFLKlEQLDQDgGFSTDVAGSgVDSTGQAGTYFYTAPEIEqDWPKIDEKADMY  685

         *******9876333333312333333335699*************87466789*******

S: 224   sLGlilfeLlypfstemervetltelrd.gkipedfddkvpkekkllqkllsrdpkeRps  282

         sLG+++feL +pf t+mer  +lt+l+  g++p ++ ++ p++++ll++l+s +p++Rps

Q: 686   SLGVVFFELWHPFGTAMERHVILTNLKLkGELPLKWVNEFPEQASLLRRLMSPSPSDRPS  745

         ***************************88*******************************

S: 283   asellksel  291

         a+ellk+++

Q: 746   ATELLKHAF  754

         ******986

Domain Sequence
(FASTA)

FEELKPLGQG GFGHVVLCKN KLDGRQYAVK KIRLKDKEIP VNSRIVREVA TLSRLQHQHV 60

VRYYQAWFET GVVDPFAGAN WGSKTAGSSM FSYSGAVSTE IPEQDNNLES TYLYIQMEYC 120

PRTLRQVFES YNHFDKDFAW HLIRQIVEGL AHIHGQGIIH RDFTPNNIFF DARNDIKIGD 180

FGLAKFLKLE QLDQDGGFST DVAGSGVDST GQAGTYFYTA PEIEQDWPKI DEKADMYSLG 240

VVFFELWHPF GTAMERHVIL TNLKLKGELP LKWVNEFPEQ ASLLRRLMSP SPSDRPSATE 300

LLKHAF 306

Keyword

Alternative splicing; Amino-acid biosynthesis; Aminoacyl-tRNA synthetase; ATP-binding; Complete proteome; Kinase; Ligase; Nucleotide-binding; Protein biosynthesis; Reference proteome; Serine/threonine-protein kinase; Transferase.

Sequence Source

Ensembl

Orthology

Ortholog group

Arabidopsis lyrata"; ?>Brachypodium distachyon"; ?>Brassica rapa"; ?>Cyanidioschyzon merolae"; ?>Glycine max"; ?>Musa acuminata"; ?>Oryza brachyantha"; ?>Oryza glaberrima"; ?>Oryza indica"; ?>Oryza sativa"; ?>Physcomitrella patens"; ?>Populus trichocarpa"; ?>Selaginella moellendorffii"; ?>Setaria italica"; ?>Solanum lycopersicum"; ?>Zea mays"; ?>

Gene Ontology

GO:0004812	; F:aminoacyl-tRNA ligase activity
GO:0005524	; F:ATP binding
GO:0004674	; F:protein serine/threonine kinase activity
GO:0004713	; F:protein tyrosine kinase activity
GO:0000049	; F:tRNA binding
GO:0008652	; P:cellular amino acid biosynthetic process
GO:0006412	; P:translation

KEGG

ath:AT3G59410

;

InterPros

IPR004154	; Anticodon-bd.
IPR024435	; GCN2_anticodon-bd_dom.
IPR011009	; Kinase-like_dom.
IPR000719	; Prot_kinase_cat_dom.
IPR017441	; Protein_kinase_ATP_BS.
IPR006575	; RWD-domain.
IPR008266	; Tyr_kinase_AS.
IPR016135	; UBQ-conjugating_enzyme/RWD.

Pfam

PF12745	; HGTP_anticodon2; 1.
PF00069	; Pkinase; 1.
PF05773	; RWD; 1.

SMARTs

SM00591

; RWD; 1.

Prosites

PS50862	; AA_TRNA_LIGASE_II; FALSE_NEG.
PS00107	; PROTEIN_KINASE_ATP; 1.
PS50011	; PROTEIN_KINASE_DOM; 1.
PS00109	; PROTEIN_KINASE_TYR; 1.
PS50908	; RWD; 1.

Prints

Created Date

20-Feb-2013