EKS-ART-00462

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EKS-ART-00462

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
CAMK/CAMKL	317.0	1.3E-94	26	280	255

Status

Reviewed

Ensembl Protein

AT4G18700.1

UniProt Accession

Q9SN43;

Protein Name

CBL-interacting serine/threonine-protein kinase 12

Protein Synonyms/Alias

SNF1-related kinase 3.9; SOS2-like protein kinase PKS8;

Gene Name

CIPK12

Gene Synonyms/Alias

CIPK12; PKS8, SnRK3.9; At4g18700; F28A21.110;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
AT4G18700	AT4G18700.1	AT4G18700.1

Organism

Arabidopsis thaliana

Functional Description

CIPK serine-threonine protein kinases interact with CBLproteins. Binding of a CBL protein to the regulatory NAF domain of CIPK protein lead to the activation of the kinase in a calcium- dependent manner (By similarity).

Protein Length

489

Protein Sequence
(FASTA)

MAEKITRETS LPKERSSPQA LILGRYEMGK LLGHGTFAKV YLARNVKTNE SVAIKVIDKE 60

KVLKGGLIAH IKREISILRR VRHPNIVQLF EVMATKAKIY FVMEYVRGGE LFNKVAKGRL 120

KEEVARKYFQ QLISAVTFCH ARGVYHRDLK PENLLLDENG NLKVSDFGLS AVSDQIRQDG 180

LFHTFCGTPA YVAPEVLARK GYDAAKVDIW SCGVILFVLM AGYLPFHDRN VMAMYKKIYR 240

GEFRCPRWFS TELTRLLSKL LETNPEKRFT FPEIMENSWF KKGFKHIKFY VEDDKLCNVV 300

DDDELESDSV ESDRDSAASE SEIEYLEPRR RVGGLPRPAS LNAFDIISFS QGFDLSGLFD 360

DDGEGSRFVS GAPVSKIISK LEEIAKVVSF TVRKKDCRVS LEGSRQGVKG PLTIAAEIFE 420

LTPSLVVVEV KKKGGDKTEY EDFCNNELKP KLQNLTADDV VAEPVAVSAV DETAIPNSPT 480

ISFLPSDTE 489

Nucleotide Sequence
(FASTA)

ATGGCGGAGA AAATCACGAG AGAGACGTCG TTACCTAAAG AGAGAAGCAG CCCACAAGCT 60

CTAATCCTGG GACGATACGA AATGGGTAAG CTTCTCGGCC ATGGTACCTT CGCTAAAGTT 120

TACCTCGCAC GTAACGTGAA AACAAACGAA AGCGTAGCAA TCAAAGTAAT CGACAAGGAG 180

AAAGTTCTCA AAGGAGGTTT AATCGCACAC ATCAAACGCG AGATCTCGAT TCTTCGACGT 240

GTTCGTCACC CAAACATCGT TCAGCTATTC GAAGTCATGG CGACGAAAGC TAAGATCTAT 300

TTCGTGATGG AGTATGTTCG TGGAGGTGAG TTATTCAATA AAGTAGCTAA AGGTCGTCTT 360

AAAGAAGAAG TAGCTCGCAA ATATTTCCAG CAATTGATCT CTGCTGTTAC TTTCTGTCAC 420

GCGAGAGGTG TTTATCATAG AGATCTGAAA CCTGAGAATC TTTTGTTAGA TGAGAATGGT 480

AATCTTAAAG TCTCTGACTT TGGACTTAGT GCTGTCTCTG ATCAGATTCG TCAAGATGGG 540

CTTTTTCATA CGTTTTGTGG TACTCCTGCT TATGTTGCTC CTGAGGTTTT AGCTAGGAAA 600

GGTTATGATG CTGCTAAAGT TGATATTTGG TCTTGTGGTG TTATCTTGTT TGTGTTGATG 660

GCTGGTTATT TGCCGTTTCA TGATCGGAAT GTTATGGCTA TGTATAAGAA GATTTACAGA 720

GGGGAGTTTA GGTGTCCTAG GTGGTTTTCT ACTGAGCTTA CCAGGTTGTT GTCGAAGCTT 780

TTGGAGACGA ATCCGGAGAA ACGGTTCACT TTCCCTGAGA TTATGGAGAA TTCTTGGTTT 840

AAGAAAGGGT TTAAGCATAT TAAGTTTTAT GTGGAGGATG ATAAGTTGTG TAATGTTGTT 900

GATGATGATG AACTGGAGTC TGACTCGGTG GAGTCGGATA GAGATTCCGC GGCTTCTGAG 960

TCGGAGATTG AGTATTTGGA GCCTAGGAGG AGAGTTGGAG GGTTGCCTAG ACCTGCGAGT 1020

TTGAATGCTT TCGATATTAT ATCGTTTTCG CAAGGTTTTG ATTTATCGGG TTTGTTTGAT 1080

GACGATGGGG AGGGTTCTAG GTTTGTTTCG GGAGCTCCGG TTTCGAAGAT TATATCGAAG 1140

TTGGAAGAGA TTGCTAAAGT TGTGAGCTTT ACTGTGAGGA AGAAGGATTG TAGGGTAAGT 1200

CTTGAAGGTT CAAGACAAGG AGTGAAAGGT CCATTGACGA TTGCAGCAGA GATATTCGAA 1260

TTGACACCAT CGTTGGTTGT TGTGGAAGTC AAGAAGAAAG GAGGAGATAA AACAGAGTAT 1320

GAAGATTTCT GTAACAATGA ATTGAAACCC AAGTTGCAAA ACTTGACAGC TGATGATGTA 1380

GTAGCTGAGC CTGTCGCGGT TTCAGCGGTT GATGAAACCG CTATCCCGAA TTCTCCAACC 1440

ATTTCTTTCT TGCCGTCTGA CACTGAATAG 1470

Domain Profile

S: 1     ykllktlGegsfgkVklakhketkekvavKiikkkkl.seeslekikrEieilkklkhpn  59

         y+++k lG+g+f+kV la++ +t+e+va+K+i+k+k+ +   + +ikrEi+il++++hpn

Q: 26    YEMGKLLGHGTFAKVYLARNVKTNESVAIKVIDKEKVlKGGLIAHIKREISILRRVRHPN  85

         99********************************9986788899****************

S: 60    iikllevietkkklylvleyasggeLfdyieekgrlkekearklfkqlvsavkYlhskgi  119

         i++l+ev+ tk k+y+v+ey++ggeLf+++ +kgrlke+ ark+f+ql+sav+++h++g+

Q: 86    IVQLFEVMATKAKIYFVMEYVRGGELFNKV-AKGRLKEEVARKYFQQLISAVTFCHARGV  144

         *****************************6.89***************************

S: 120   vHrDlKpeNllldkkenikiaDFGlan...lfkegklletfcGsppYaaPellkgkkYeg  176

          HrDlKpeNllld+++n+k++DFGl++   +++++ l++tfcG+p+Y+aPe+l++k Y++

Q: 145   YHRDLKPENLLLDENGNLKVSDFGLSAvsdQIRQDGLFHTFCGTPAYVAPEVLARKGYDA  204

         *************************9977788899*************************

S: 177   pkvDvWslGvvLyalvtgklPfdeenlkellekilkgkleipkklskelesLlrklLvvd  236

          kvD+Ws+Gv+L++l++g lPf+++n+ ++++ki +g++++p + s+el++Ll+klL+++

Q: 205   AKVDIWSCGVILFVLMAGYLPFHDRNVMAMYKKIYRGEFRCPRWFSTELTRLLSKLLETN  264

         ************************************************************

S: 237   pekRatieeilkhewl  252

         pekR+t  ei++++w+

Q: 265   PEKRFTFPEIMENSWF  280

         ***************6

Domain Sequence
(FASTA)

YEMGKLLGHG TFAKVYLARN VKTNESVAIK VIDKEKVLKG GLIAHIKREI SILRRVRHPN 60

IVQLFEVMAT KAKIYFVMEY VRGGELFNKV AKGRLKEEVA RKYFQQLISA VTFCHARGVY 120

HRDLKPENLL LDENGNLKVS DFGLSAVSDQ IRQDGLFHTF CGTPAYVAPE VLARKGYDAA 180

KVDIWSCGVI LFVLMAGYLP FHDRNVMAMY KKIYRGEFRC PRWFSTELTR LLSKLLETNP 240

EKRFTFPEIM ENSWF 255

Keyword

ATP-binding; Complete proteome; Kinase; Manganese; Nucleotide-binding; Reference proteome; Serine/threonine-protein kinase; Transferase.

Sequence Source

Ensembl

Orthology

Ortholog group

Arabidopsis lyrata"; ?>Brachypodium distachyon"; ?>Brassica rapa"; ?>Glycine max"; ?>Hordeum vulgare"; ?>Oryza brachyantha"; ?>Oryza glaberrima"; ?>Oryza indica"; ?>Oryza sativa"; ?>Populus trichocarpa"; ?>Solanum lycopersicum"; ?>Solanum tuberosum"; ?>Sorghum bicolor"; ?>Vitis vinifera"; ?>Zea mays"; ?>

Gene Ontology

GO:0005524	; F:ATP binding
GO:0004674	; F:protein serine/threonine kinase activity
GO:0007165	; P:signal transduction

KEGG

ath:AT4G18700

;

InterPros

IPR011009	; Kinase-like_dom.
IPR018451	; NAF/FISL_domain.
IPR004041	; NAF_dom.
IPR000719	; Prot_kinase_cat_dom.
IPR017441	; Protein_kinase_ATP_BS.
IPR002290	; Ser/Thr_dual-sp_kinase_dom.
IPR008271	; Ser/Thr_kinase_AS.

Pfam

PF03822	; NAF; 1.
PF00069	; Pkinase; 1.

SMARTs

SM00220

; S_TKc; 1.

Prosites

PS50816	; NAF; 1.
PS00107	; PROTEIN_KINASE_ATP; 1.
PS50011	; PROTEIN_KINASE_DOM; 1.
PS00108	; PROTEIN_KINASE_ST; 1.

Prints

Created Date

20-Feb-2013