EKS-ART-00138

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EKS-ART-00138

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
TKL/IRAK	224.4	3.5E-66	86	360	275

Status

Reviewed

Ensembl Protein

AT5G13160.1

UniProt Accession

Q9FE20;

Protein Name

Serine/threonine-protein kinase PBS1

Protein Synonyms/Alias

AvrPphB susceptible protein 1;

Gene Name

PBS1

Gene Synonyms/Alias

PBS1; At5g13160; T19L5.120;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
AT5G13160	AT5G13160.1	AT5G13160.1

Organism

Arabidopsis thaliana

Functional Description

Protein kinase required for plant defense mechanismmediated by the disease resistance (R) protein RPS5. In case of infection by Pseudomonas syringae, RPS5 specifically recognizes the avrPphB type III effector avirulence protein and triggers a defense reaction. AvrPphB may trigger RPS5-mediated defense mechanism via the cleavage of PBS1. Both kinase activity and cleavage by avrPphB are independently required to trigger the RPS5-mediated resistance.

Protein Length

456

Protein Sequence
(FASTA)

MGCFSCFDSS DDEKLNPVDE SNHGQKKQSQ PTVSNNISGL PSGGEKLSSK TNGGSKRELL 60

LPRDGLGQIA AHTFAFRELA AATMNFHPDT FLGEGGFGRV YKGRLDSTGQ VVAVKQLDRN 120

GLQGNREFLV EVLMLSLLHH PNLVNLIGYC ADGDQRLLVY EFMPLGSLED HLHDLPPDKE 180

ALDWNMRMKI AAGAAKGLEF LHDKANPPVI YRDFKSSNIL LDEGFHPKLS DFGLAKLGPT 240

GDKSHVSTRV MGTYGYCAPE YAMTGQLTVK SDVYSFGVVF LELITGRKAI DSEMPHGEQN 300

LVAWARPLFN DRRKFIKLAD PRLKGRFPTR ALYQALAVAS MCIQEQAATR PLIADVVTAL 360

SYLANQAYDP SKDDSRRNRD ERGARLITRN DDGGGSGSKF DLEGSEKEDS PRETARILNR 420

DINRERAVAE AKMWGESLRE KRRQSEQGTS ESNSTG 456

Nucleotide Sequence
(FASTA)

ATGGGTTGTT TCTCGTGTTT TGATTCGAGT GATGACGAGA AGCTGAATCC AGTTGATGAA 60

TCTAATCATG GTCAGAAGAA ACAATCACAA CCGACAGTAT CCAATAACAT ATCTGGACTC 120

CCTTCAGGTG GGGAGAAGCT TAGCTCAAAG ACCAATGGAG GATCAAAAAG GGAGCTACTG 180

CTTCCAAGGG ATGGACTTGG ACAAATTGCT GCTCATACAT TTGCTTTCCG CGAGCTTGCT 240

GCTGCAACTA TGAACTTTCA TCCTGACACT TTCTTAGGCG AAGGTGGATT TGGACGTGTC 300

TACAAAGGAA GGCTTGACAG CACCGGTCAG GTTGTTGCTG TTAAACAACT AGACAGGAAT 360

GGTCTACAAG GTAACAGAGA ATTTCTGGTA GAGGTTCTTA TGCTCAGTCT TCTTCATCAT 420

CCCAACTTAG TCAACCTTAT TGGTTATTGT GCTGATGGAG ATCAACGCCT CTTGGTCTAC 480

GAGTTTATGC CGTTAGGATC ATTGGAAGAT CACCTCCACG ATCTTCCACC GGATAAGGAG 540

GCCTTAGATT GGAACATGAG AATGAAAATA GCTGCTGGTG CGGCGAAAGG ATTGGAATTT 600

CTACATGATA AGGCAAACCC TCCGGTTATT TATAGAGATT TTAAGTCATC AAATATTTTA 660

CTGGATGAGG GTTTCCACCC TAAGCTTTCT GATTTTGGAC TTGCTAAACT CGGACCAACG 720

GGAGACAAAT CTCATGTCTC CACTAGAGTT ATGGGAACTT ATGGTTATTG TGCTCCCGAG 780

TACGCAATGA CGGGACAATT GACAGTAAAA TCAGATGTCT ACAGTTTTGG TGTGGTTTTT 840

CTCGAGCTCA TTACTGGTCG CAAAGCTATA GACAGCGAGA TGCCTCATGG AGAGCAGAAC 900

CTGGTGGCTT GGGCTCGCCC ATTGTTCAAC GACAGGCGAA AGTTCATAAA ACTGGCTGAT 960

CCAAGGTTAA AGGGGCGGTT TCCAACGCGT GCACTCTACC AAGCTTTAGC TGTGGCATCA 1020

ATGTGCATCC AAGAACAGGC GGCTACACGT CCTCTCATAG CAGATGTTGT CACTGCACTC 1080

TCCTATCTTG CAAACCAAGC TTATGATCCA AGTAAAGATG ATAGTAGAAG AAACCGGGAT 1140

GAAAGAGGTG CAAGGTTAAT AACAAGGAAC GACGATGGAG GTGGCTCGGG AAGTAAATTC 1200

GATTTAGAAG GTTCAGAGAA AGAAGATTCA CCGAGAGAGA CAGCTCGGAT ATTGAACCGA 1260

GATATCAATA GGGAGCGTGC GGTTGCAGAG GCTAAGATGT GGGGAGAGAG TTTGAGGGAG 1320

AAACGAAGAC AGAGCGAGCA GGGTACTTCA GAGAGCAACA GTACCGGGTA G 1371

Domain Profile

S: 1     fsednrigeGgfgevyrgelknt..avavkklkeeadlslkelkqsfltElkvlarfrHd  58

         f+ d+ +geGgfg vy+g+l  t  +vavk+l  +      + +++fl E+ +l+ ++H+

Q: 86    FHPDTFLGEGGFGRVYKGRLDSTgqVVAVKQLDRNG----LQGNREFLVEVLMLSLLHHP  141

         788999************99876669*****98765....34578999************

S: 59    nilellgysaeseklcLvYqymknGsLedrLqcq.kgsepLsWpqRlsillGtaraiefL  117

         n+++l+gy+a++++  LvY++m+ GsLed+L+    ++e L W++R++i+ G+a+++efL

Q: 142   NLVNLIGYCADGDQRLLVYEFMPLGSLEDHLHDLpPDKEALDWNMRMKIAAGAAKGLEFL  201

         ********************************98799***********************

S: 118   Heasp.slihgdiksaNiLLDekltpKlgDfglarfapesekqsstvlrtskvrgtlaYl  176

         H++ + ++i +d ks+NiLLDe ++pKl+Dfgla++ p+ +k+  +v    +v gt  Y

Q: 202   HDKANpPVIYRDFKSSNILLDEGFHPKLSDFGLAKLGPTGDKS--HVS--TRVMGTYGYC  257

         *99888*****************************77766654..343..5789******

S: 177   peefirvgqltvkvDvySfGiVllEvltGlravded..rktkyLkdllkeeieeekveil  234

         ++e+  +gqltvk+DvySfG+V lE++tG++a+d +  + +++L+   +  +++++ + +

Q: 258   APEYAMTGQLTVKSDVYSFGVVFLELITGRKAIDSEmpHGEQNLVAWARPLFNDRR-KFI  316

         ********************************776611456889999899999888.555

S: 235   ekfldkkagkleeellealielalaclaekakkrPtmsqvlell  278

         +    +  g+ ++  l +++ +a+ c++e+a +rP +++v+  l

Q: 317   KLADPRLKGRFPTRALYQALAVASMCIQEQAATRPLIADVVTAL  360

         44434445788999999999999****************99765

Domain Sequence
(FASTA)

FHPDTFLGEG GFGRVYKGRL DSTGQVVAVK QLDRNGLQGN REFLVEVLML SLLHHPNLVN 60

LIGYCADGDQ RLLVYEFMPL GSLEDHLHDL PPDKEALDWN MRMKIAAGAA KGLEFLHDKA 120

NPPVIYRDFK SSNILLDEGF HPKLSDFGLA KLGPTGDKSH VSTRVMGTYG YCAPEYAMTG 180

QLTVKSDVYS FGVVFLELIT GRKAIDSEMP HGEQNLVAWA RPLFNDRRKF IKLADPRLKG 240

RFPTRALYQA LAVASMCIQE QAATRPLIAD VVTAL 275

Keyword

ATP-binding; Complete proteome; Direct protein sequencing; Kinase; Nucleotide-binding; Phosphoprotein; Plant defense; Reference proteome; Serine/threonine-protein kinase; Transferase.

Sequence Source

Ensembl

Orthology

Ortholog group

Arabidopsis lyrata"; ?>Brachypodium distachyon"; ?>Brassica rapa"; ?>Glycine max"; ?>Hordeum vulgare"; ?>Musa acuminata"; ?>Oryza brachyantha"; ?>Oryza glaberrima"; ?>Oryza indica"; ?>Oryza sativa"; ?>Populus trichocarpa"; ?>Setaria italica"; ?>Solanum lycopersicum"; ?>Solanum tuberosum"; ?>Sorghum bicolor"; ?>Vitis vinifera"; ?>Zea mays"; ?>

Gene Ontology

GO:0005524	; F:ATP binding
GO:0004674	; F:protein serine/threonine kinase activity
GO:0009816	; P:defense response to bacterium, incompatible interaction
GO:0046777	; P:protein autophosphorylation

KEGG

ath:AT5G13160

;

InterPros

IPR011009	; Kinase-like_dom.
IPR000719	; Prot_kinase_cat_dom.
IPR017441	; Protein_kinase_ATP_BS.
IPR008271	; Ser/Thr_kinase_AS.

Pfam

PF00069

; Pkinase; 1.

SMARTs

Prosites

PS00107	; PROTEIN_KINASE_ATP; 1.
PS50011	; PROTEIN_KINASE_DOM; 1.
PS00108	; PROTEIN_KINASE_ST; 1.

Prints

Created Date

20-Feb-2013