EKS-CAE-00172

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EKS-CAE-00172

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
CMGC/SRPK	774.9	5.3E-233	134	616	483

Status

Reviewed

Ensembl Protein

B0464.5b.1

UniProt Accession

Q03563; Q9XVX9; Q9XVY0;

Protein Name

Serine/threonine-protein kinase spk-1

Protein Synonyms/Alias

Gene Name

spk-1

Gene Synonyms/Alias

spk-1; rsk-1, srk-1; B0464.5;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
B0464.5	B0464.5a.2	B0464.5a.2
B0464.5	B0464.5b.1	B0464.5b.1
B0464.5	B0464.5a.1	B0464.5a.1
B0464.5	B0464.5b.2	B0464.5b.2
B0464.5	B0464.5c.1	B0464.5c.1
B0464.5	B0464.5c.3	B0464.5c.3
B0464.5	B0464.5c.2	B0464.5c.2

Organism

Caenorhabditis elegans

Functional Description

Required for embryogenesis and germline development inboth adult hermaphrodites and males. SR-protein kinase (SRPK) that binds directly to and phosphorylates the RS domain of rsp-3/CeSF2 in vitro.

Protein Length

698

Protein Sequence
(FASTA)

MGGPEKNSTV PLPNKKRKKK TNKKKPTAPN TPTSPQAGEK NANLKNGTVT TNGSNHVERT 60

EVNANEERLD DLSVSPGRSD SPGGGGGGHS DSFQDPMDPG EQLGSDDEEQ EDPRDYKRGG 120

YHPVNIGDVF NARYHVIRKL GWGHFSTVWL AWDTQDKRFV AMKIVKSAEH YTEAALDEIK 180

LLLSVRSADP NDIGCHKVVQ LLDEFTVTGI NGQHVAMVFE VLGCNLLKLI IRSNYRGLHL 240

EQVRKICRQV LEALGYMHEK CGIIHTDIKP ENVLITMSRE EIKIMAQHAV VARKMNMKMS 300

GSAVSTAPDH LVKMAQENMT KNKKKKMKKK AKKQREKLEA ELAGLEGLKM DANGLQEAYN 360

NAPELENFNA SQVEDVTMED TVNENGNRNK VEIRSPDRFD RTTLTPFSDP ESKFGDLASP 420

SAEYLSSPMS QLPPGGILPA PPVGPNIGDP YCDIDVKIAD LGNACWVNHH YTDDIQTRQY 480

RALEVLIGSG YGPPADIWST ACMAFELATG DYLFEPHQGD NYSRDEDHLA HISELLGAIP 540

PSIYKKGKHW REFFHKNGHL LHIHQLKPWS LYEVLRQKYE WSHEDAQQFE SFLRPMLDFD 600

QEKRSTAKIA LKHPFLLPFG GRAPKSDCPP ELLSKMFPDG LIPEPFDGNE HQEVYRDEND 660

SRSASERSAN SRSAGGSDDD DEEEFNMNRP GPSGLDLQ 698

Nucleotide Sequence
(FASTA)

ATGGGCGGAC CAGAAAAAAA CAGCACAGTA CCACTGCCAA ATAAGAAAAG GAAGAAGAAG 60

ACCAATAAGA AAAAGCCCAC AGCACCAAAC ACGCCTACAT CTCCTCAAGC GGGTGAGAAA 120

AACGCAAATT TGAAAAATGG AACAGTTACA ACAAACGGAT CAAATCACGT GGAAAGGACC 180

GAAGTCAATG CTAATGAAGA GCGTTTGGAC GATTTAAGTG TGAGCCCCGG ACGAAGTGAT 240

TCTCCTGGAG GCGGCGGTGG TGGACATTCA GATTCATTCC AAGATCCAAT GGATCCTGGA 300

GAACAACTTG GATCTGACGA TGAAGAACAA GAAGATCCAC GTGATTACAA ACGAGGTGGA 360

TATCATCCAG TCAACATTGG TGATGTATTC AATGCGAGAT ATCATGTGAT TCGTAAACTT 420

GGATGGGGTC ACTTTTCTAC GGTTTGGCTG GCGTGGGACA CTCAAGACAA ACGATTTGTT 480

GCCATGAAAA TAGTGAAGTC AGCGGAGCAT TACACGGAAG CGGCTCTTGA TGAAATTAAG 540

TTGTTGCTAT CCGTACGAAG TGCTGATCCA AATGACATCG GATGCCACAA GGTTGTTCAA 600

TTGCTCGATG AATTCACTGT TACGGGAATT AATGGTCAGC ACGTTGCTAT GGTGTTTGAA 660

GTTCTTGGGT GCAATCTGTT AAAGCTGATT ATACGCTCAA ATTATCGAGG ACTTCATCTG 720

GAACAAGTTA GGAAAATATG CAGACAAGTT CTGGAGGCGT TAGGATATAT GCATGAAAAG 780

TGCGGCATCA TTCATACTGA TATTAAACCT GAAAATGTGC TAATCACGAT GAGCCGAGAA 840

GAGATCAAGA TTATGGCTCA ACATGCTGTA GTTGCCCGGA AGATGAATAT GAAAATGAGT 900

GGATCGGCTG TCAGTACTGC ACCGGATCAT TTGGTGAAAA TGGCTCAGGA GAATATGACG 960

AAAAACAAGA AGAAAAAGAT GAAGAAGAAG GCCAAAAAAC AACGAGAGAA GTTGGAAGCG 1020

GAATTGGCTG GACTCGAAGG CCTCAAAATG GACGCTAATG GGCTGCAGGA GGCATACAAT 1080

AATGCACCGG AGTTGGAGAA CTTCAACGCT TCACAAGTGG AAGATGTCAC CATGGAAGAT 1140

ACTGTCAATG AAAATGGAAA CAGAAACAAG GTGGAAATCA GGAGCCCTGA TCGTTTCGAT 1200

AGAACCACGC TGACTCCATT CAGTGATCCG GAGAGCAAGT TTGGAGATTT GGCCTCACCA 1260

TCCGCCGAAT ATCTGAGCTC GCCCATGAGC CAGTTGCCTC CAGGTGGTAT CCTGCCTGCT 1320

CCTCCAGTTG GCCCCAACAT TGGTGATCCA TATTGTGACA TCGACGTCAA AATCGCTGAT 1380

CTCGGAAACG CGTGTTGGGT GAATCATCAC TACACGGACG ATATTCAAAC TCGTCAGTAT 1440

AGAGCACTTG AAGTGTTGAT CGGTTCAGGA TACGGACCTC CAGCTGATAT TTGGAGTACC 1500

GCGTGTATGG CTTTTGAATT GGCAACCGGT GACTATTTGT TTGAACCACA CCAAGGAGAT 1560

AACTATTCAC GCGATGAAGA TCATCTTGCG CACATTTCCG AACTTCTTGG AGCAATTCCA 1620

CCGTCAATTT ACAAAAAGGG TAAACATTGG AGAGAGTTCT TCCACAAAAA TGGACACTTA 1680

CTTCACATTC ACCAACTTAA ACCATGGTCA CTATACGAGG TTTTGAGACA AAAATACGAG 1740

TGGTCTCATG AAGACGCGCA GCAGTTTGAA AGTTTCCTTC GTCCGATGCT TGATTTCGAT 1800

CAGGAGAAAC GATCTACGGC CAAAATTGCA TTGAAACATC CGTTCTTGTT ACCATTTGGA 1860

GGAAGAGCGC CAAAATCTGA TTGTCCACCT GAGCTTCTGA GCAAGATGTT TCCGGATGGA 1920

TTGATTCCCG AGCCGTTCGA CGGAAATGAG CATCAGGAAG TTTACCGTGA CGAAAATGAT 1980

AGTAGATCGG CGAGCGAGAG GAGTGCCAAT AGCAGGTCTG CGGGCGGCAG CGACGACGAC 2040

GACGAGGAGG AGTTCAACAT GAATCGACCT GGTCCATCGG GGCTGGATTT GCAGTAG 2097

Domain Profile

S: 1     yhvirkLGWGhfstvWLawdlqekrfvalkvvksaehytetaldeikllksvresdpsdp  60

         yhvirkLGWGhfstvWLawd+q+krfva+k+vksaehyte+aldeikll svr++dp+d+

Q: 134   YHVIRKLGWGHFSTVWLAWDTQDKRFVAMKIVKSAEHYTEAALDEIKLLLSVRSADPNDI  193

         9***********************************************************

S: 61    krekvvqllddfkisGvnGvhvclvfevLGenLlkliiksnyrGlpleqvkkiirqvLeg  120

          ++kvvqlld+f+++G+nG+hv++vfevLG+nLlklii+snyrGl+leqv+ki+rqvLe+

Q: 194   GCHKVVQLLDEFTVTGINGQHVAMVFEVLGCNLLKLIIRSNYRGLHLEQVRKICRQVLEA  253

         ************************************************************

S: 121   LdylhekCkiihtdikPenvLlavddeyirrlaaeatewqkagakppsgsavstapq...  177

         L+y+hekC+iihtdikPenvL+++++e+i+++a++a++++k+++k +sgsavstap+

Q: 254   LGYMHEKCGIIHTDIKPENVLITMSREEIKIMAQHAVVARKMNMK-MSGSAVSTAPDhlv  312

         ********************************************9.***********888

S: 178   kkaieklsknkkkklkkkqkrqaellekrlqeleeleaaeanseaedqelkedaekgele  237

         k+a+e+++knkkkk+kkk+k+q+e+le++l++le l++  an+ +e       ++  ele

Q: 313   KMAQENMTKNKKKKMKKKAKKQREKLEAELAGLEGLKMD-ANGLQE-----AYNNAPELE  366

         89**********************************864.343333.....234567899

S: 238   eieadveeevanedeaseeee.............essltsssdaesksgelseastells  284

         +++a+++e+v++ed+++e+++             +++lt++sd+esk+g+l+++s+e+ls

Q: 367   NFNASQVEDVTMEDTVNENGNrnkveirspdrfdRTTLTPFSDPESKFGDLASPSAEYLS  426

         999999*******9999999999999**********************************

S: 285   esleqlacgsvlsvnplepknad...kikvkiadLGnaCwvhkhftediqtrqyrslevl  341

         ++++ql +g++l+++p++p+++d   +i+vkiadLGnaCwv++h+t+diqtrqyr+levl

Q: 427   SPMSQLPPGGILPAPPVGPNIGDpycDIDVKIADLGNACWVNHHYTDDIQTRQYRALEVL  486

         ***********************9999*********************************

S: 342   iGagygtpadiWstaClafeLatGdyLfephsgedysrdedhiakiiellGkiPrklilk  401

         iG+gyg+padiWstaC+afeLatGdyLfeph+g++ysrdedh+a+i ellG+iP+++++k

Q: 487   IGSGYGPPADIWSTACMAFELATGDYLFEPHQGDNYSRDEDHLAHISELLGAIPPSIYKK  546

         ************************************************************

S: 402   GkysreffnkkgeLrhitkLkpwsLlevLvekyewskeeaaefsdfLlPmleldpekras  461

         Gk++reff+k+g+L hi++LkpwsL+evL +kyews+e+a++f++fL+Pml++d+ekr++

Q: 547   GKHWREFFHKNGHLLHIHQLKPWSLYEVLRQKYEWSHEDAQQFESFLRPMLDFDQEKRST  606

         ************************************************************

S: 462   aaeclkhpwL  471

         a+ +lkhp+L

Q: 607   AKIALKHPFL  616

         *********8

Domain Sequence
(FASTA)

YHVIRKLGWG HFSTVWLAWD TQDKRFVAMK IVKSAEHYTE AALDEIKLLL SVRSADPNDI 60

GCHKVVQLLD EFTVTGINGQ HVAMVFEVLG CNLLKLIIRS NYRGLHLEQV RKICRQVLEA 120

LGYMHEKCGI IHTDIKPENV LITMSREEIK IMAQHAVVAR KMNMKMSGSA VSTAPDHLVK 180

MAQENMTKNK KKKMKKKAKK QREKLEAELA GLEGLKMDAN GLQEAYNNAP ELENFNASQV 240

EDVTMEDTVN ENGNRNKVEI RSPDRFDRTT LTPFSDPESK FGDLASPSAE YLSSPMSQLP 300

PGGILPAPPV GPNIGDPYCD IDVKIADLGN ACWVNHHYTD DIQTRQYRAL EVLIGSGYGP 360

PADIWSTACM AFELATGDYL FEPHQGDNYS RDEDHLAHIS ELLGAIPPSI YKKGKHWREF 420

FHKNGHLLHI HQLKPWSLYE VLRQKYEWSH EDAQQFESFL RPMLDFDQEK RSTAKIALKH 480

PFL 483

Keyword

Alternative splicing; ATP-binding; Complete proteome; Developmental protein; Kinase; Nucleotide-binding; Reference proteome; Serine/threonine-protein kinase; Transferase.

Sequence Source

Ensembl

Orthology

Ortholog group

Ailuropoda melanoleuca"; ?>Anolis carolinensis"; ?>Bos taurus"; ?>Callithrix jacchus"; ?>Canis familiaris"; ?>Cavia porcellus"; ?>Choloepus hoffmanni"; ?>Ciona intestinalis"; ?>Ciona savignyi"; ?>Danio rerio"; ?>Dipodomys ordii"; ?>Drosophila melanogaster"; ?>Echinops telfairi"; ?>Equus caballus"; ?>Felis catus"; ?>Gadus morhua"; ?>Gallus gallus"; ?>Gasterosteus aculeatus"; ?>Gorilla gorilla"; ?>Homo sapiens"; ?>Ictidomys tridecemlineatus"; ?>Latimeria chalumnae"; ?>Loxodonta africana"; ?>Macaca mulatta"; ?>Meleagris gallopavo"; ?>Microcebus murinus"; ?>Monodelphis domestica"; ?>Mus musculus"; ?>Mustela putorius furo"; ?>Myotis lucifugus"; ?>Nomascus leucogenys"; ?>Oreochromis niloticus"; ?>Ornithorhynchus anatinus"; ?>Oryctolagus cuniculus"; ?>Oryzias latipes"; ?>Otolemur garnettii"; ?>Pan troglodytes"; ?>Pelodiscus sinensis"; ?>Petromyzon marinus"; ?>Pongo abelii"; ?>Pteropus vampyrus"; ?>Rattus norvegicus"; ?>Sarcophilus harrisii"; ?>Sorex araneus"; ?>Sus scrofa"; ?>Taeniopygia guttata"; ?>Takifugu rubripes"; ?>Tetraodon nigroviridis"; ?>Tupaia belangeri"; ?>Xenopus tropicalis"; ?>Xiphophorus maculatus"; ?>Saccharomyces cerevisiae"; ?>Schizosaccharomyces pombe"; ?>

Gene Ontology

GO:0005524	; F:ATP binding
GO:0004674	; F:protein serine/threonine kinase activity
GO:0000910	; P:cytokinesis
GO:0009792	; P:embryo development ending in birth or egg hatching
GO:0007276	; P:gamete generation
GO:0051729	; P:germline cell cycle switching, mitotic to meiotic cell cycle
GO:0040035	; P:hermaphrodite genitalia development
GO:0002119	; P:nematode larval development

KEGG

cel:CELE_B0464.5

;

InterPros

IPR011009	; Kinase-like_dom.
IPR000719	; Prot_kinase_cat_dom.
IPR017441	; Protein_kinase_ATP_BS.
IPR008271	; Ser/Thr_kinase_AS.

Pfam

PF00069

; Pkinase; 2.

SMARTs

Prosites

PS00107	; PROTEIN_KINASE_ATP; 1.
PS50011	; PROTEIN_KINASE_DOM; 1.
PS00108	; PROTEIN_KINASE_ST; 1.

Prints

Created Date

20-Feb-2013