EKS-CAE-00377

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EKS-CAE-00377

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
AGC/DMPK	438.0	1.9E-131	68	330	263

Status

Reviewed

Ensembl Protein

C10H11.9

UniProt Accession

P92199;

Protein Name

Rho-associated protein kinase let-502

Protein Synonyms/Alias

Lethal protein 502; Rho-binding kinase let-502;

Gene Name

let-502

Gene Synonyms/Alias

let-502; C10H11.9;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
C10H11.9	C10H11.9	C10H11.9

Organism

Caenorhabditis elegans

Functional Description

Negatively regulates mel-11 to relieve the inhibition ofmlc-4, allowing contraction of the circumferentially oriented microfilaments in epidermal cells and thereby regulating myosin II contractility during spermathecal contraction, cleavage furrow contraction in early embryos, and embryonic elongation and morphogenesis. Required for P-cell migration. May also play a role in oocyte cellularization.

Protein Length

1173

Protein Sequence
(FASTA)

MEQDELRDQL VDPKSPINIE SLLDTITALV NDCKIPVLMR MKSVDNFISR YERVVESLAA 60

LRMKAADFRQ LKVIGRGAFG EVHLVRHTRT NTVYAMKMLN KDDMIKRADS AFFWEERDIM 120

AHANSEWIVR LQYAFQDPRH LYMVMEYMPG GDLVNLMTSY EVSEKWTRFY TAEIVEALAA 180

LHSMGYIHRD VKPDNMLISI SGHIKLADFG TCVKMNANGV VRCSTAVGTP DYISPEVLRN 240

QGQDAEFGKE VDWWSVGVFI YEMLVGETPF YAEALVSTYT NIMNHKTSLK FPDEPLISTQ 300

AKDIIKKFLS AAPDRLGRNS VDDIRNHKFF VNDEWTFATL REASPPVIPS LKSDDDTTHF 360

EEIETRDRDN AGDFQLPKTF NGNQLPFIGF TYSNEYSPVK NLLKGHGAGS KQNGIEQHKP 420

QTVVEQPLTN GHASGVPEEK YEAVKMELDS KNREFELLKD SIARNEIRAK MIENEKNSLS 480

TKISDLEREL KDNKDKLRHG ADSDAKVNEL AVELRMSKEY NSEMESELSK FRDKCEQLKE 540

DLRKKSGELA QEKNETQRVF QQKKDADEAF AEIKRDYELL QTRENEKSVQ LKKALDERKE 600

NGAYQQSVAK ATDAEWERKM QFYEKQLEHA NDERKREEQK RTAAEFDQSR VARKLAGIEA 660

NYELLQNDYK SMKEARKDLE RDLQDVITEK RRLEIRVEQL MDSRNTDERV LSLCQDELVE 720

SQEEAKYKED GLRGKIDGFK HELENEKMKT QTLEENLLVA DKERGMLKME VQELMQRHKW 780

EITNKDQTLK HLETQLDEIK QQSKIESSEQ ESNDKQTIAD LRKKLDLEKA HKKAVINKLE 840

EEMAKRQPLK KGEKGVTKSA LIKKEREIMA LEQERDTMSK RIAALFYEND KQAEHFNIAI 900

QDMQTTQDAL RDELKECKEE LANRNVNTRY EDKRSLDSRE GIPSSLSNQH IQMEGWLSLR 960

DNTKKSRKPK WTNCFVALNE YSFTIYVDEK AVSVILLIEA GAMAHVRHVT AADLRNVDDS 1020

QLPKIFHIMY DDASCNSSRH ASNSDLSMIE SFREESWKRH DFQELSYHIR TFCDVCNKKL 1080

SDIIRPTPAF ECKNCHFKTH KDHVAQGSLP MCRYNTGLSR ELVLMAPQTD NCNKWVSQLR 1140

RFIESSRSAA VSVSRVSSRR HAPGSSNSST IYQ 1173

Nucleotide Sequence
(FASTA)

ATGGAGCAGG ATGAGCTGCG TGATCAGCTG GTGGATCCAA AATCCCCGAT AAACATCGAA 60

AGCTTGCTCG ACACCATCAC AGCGCTCGTC AATGATTGCA AAATTCCGGT GCTCATGCGG 120

ATGAAAAGCG TCGACAACTT CATTAGCAGA TATGAACGAG TTGTCGAAAG TTTGGCCGCT 180

CTTCGAATGA AAGCTGCCGA TTTCAGACAA TTGAAGGTTA TTGGAAGAGG AGCATTCGGA 240

GAAGTTCATC TGGTTCGACA CACACGAACG AACACTGTGT ACGCGATGAA GATGCTCAAT 300

AAGGATGACA TGATCAAACG AGCTGACTCT GCATTCTTCT GGGAGGAACG TGATATTATG 360

GCTCACGCGA ATAGCGAATG GATCGTGAGG CTTCAGTATG CATTCCAGGA TCCTCGTCAT 420

TTGTACATGG TGATGGAATA TATGCCAGGT GGAGATCTCG TGAATCTGAT GACATCCTAT 480

GAAGTATCGG AAAAGTGGAC TCGCTTTTAT ACGGCTGAAA TTGTCGAGGC GCTGGCCGCC 540

CTTCACAGTA TGGGATATAT TCATCGAGAT GTGAAGCCGG ACAATATGCT CATTTCGATC 600

AGCGGACACA TAAAATTGGC AGATTTTGGA ACATGCGTGA AGATGAATGC CAATGGAGTT 660

GTGCGATGCT CCACAGCAGT CGGAACTCCT GATTACATAT CACCGGAAGT TTTGAGAAAT 720

CAAGGACAAG ATGCTGAATT TGGAAAAGAA GTCGATTGGT GGTCTGTTGG TGTGTTCATA 780

TATGAAATGC TTGTTGGAGA AACACCATTC TATGCCGAGG CACTTGTATC AACTTATACA 840

AATATTATGA ATCATAAAAC AAGTCTGAAA TTTCCTGATG AGCCATTAAT TTCTACCCAA 900

GCCAAGGATA TTATCAAGAA ATTCCTCTCG GCGGCTCCAG ATCGTCTTGG AAGAAATAGC 960

GTCGATGATA TTCGAAATCA CAAGTTTTTT GTTAATGACG AATGGACATT TGCCACTCTC 1020

AGAGAAGCAT CTCCACCGGT CATTCCTTCA TTAAAATCGG ACGATGACAC GACACATTTC 1080

GAAGAAATCG AGACCCGTGA TCGAGATAAT GCGGGAGACT TTCAATTGCC GAAGACATTT 1140

AACGGAAATC AACTACCATT CATCGGCTTT ACTTATTCGA ACGAGTACAG TCCTGTGAAG 1200

AATCTTCTGA AGGGCCATGG TGCTGGATCA AAACAGAACG GAATTGAACA GCATAAACCG 1260

CAAACTGTTG TTGAGCAACC ATTGACGAAT GGGCATGCGT CTGGTGTTCC TGAAGAAAAA 1320

TATGAAGCAG TTAAGATGGA GTTGGACTCG AAAAACCGAG AATTCGAACT TTTGAAGGAT 1380

AGCATTGCTC GAAATGAGAT CCGAGCGAAG ATGATCGAAA ATGAGAAGAA CTCATTGTCC 1440

ACAAAAATCT CCGATCTCGA ACGAGAACTC AAGGATAATA AAGATAAACT GAGGCATGGC 1500

GCTGATTCGG ATGCAAAAGT TAACGAATTG GCTGTCGAGT TACGAATGTC CAAAGAGTAC 1560

AATAGTGAGA TGGAGAGCGA ATTGAGCAAG TTTCGGGACA AGTGTGAGCA GCTCAAGGAA 1620

GATCTTCGCA AAAAATCTGG AGAGCTTGCT CAGGAGAAGA ATGAAACTCA ACGGGTATTC 1680

CAGCAGAAGA AGGACGCTGA TGAAGCATTT GCCGAGATTA AACGGGATTA TGAACTATTA 1740

CAAACCAGAG AAAATGAAAA GTCTGTTCAA TTGAAGAAAG CGCTTGACGA ACGAAAAGAG 1800

AACGGAGCCT ATCAGCAAAG TGTTGCAAAG GCGACAGATG CTGAATGGGA GCGAAAAATG 1860

CAGTTTTATG AGAAGCAGTT GGAGCATGCG AATGATGAAC GGAAAAGAGA AGAGCAAAAG 1920

CGAACGGCTG CAGAATTCGA TCAAAGCAGG GTGGCAAGGA AATTGGCTGG GATTGAAGCG 1980

AATTATGAGC TGCTGCAGAA TGACTATAAA AGTATGAAGG AAGCAAGGAA GGATCTTGAA 2040

AGAGATCTTC AGGATGTCAT TACTGAGAAA AGGCGACTTG AAATACGTGT CGAACAGTTG 2100

ATGGACAGCC GGAACACCGA CGAACGGGTA TTATCGCTCT GTCAGGATGA GCTCGTGGAA 2160

AGTCAAGAGG AAGCAAAGTA TAAGGAAGAT GGATTGAGAG GAAAGATTGA CGGATTTAAA 2220

CATGAACTTG AGAATGAGAA GATGAAGACT CAAACTTTGG AGGAAAATCT TCTGGTAGCT 2280

GATAAGGAGA GGGGCATGCT GAAAATGGAA GTTCAAGAGC TGATGCAAAG GCATAAATGG 2340

GAGATTACCA ACAAAGATCA AACGCTGAAA CATCTTGAGA CTCAACTAGA CGAAATAAAG 2400

CAACAATCAA AGATCGAGTC GTCTGAGCAG GAATCGAATG ATAAGCAGAC GATCGCTGAT 2460

CTTCGAAAGA AGTTGGATCT AGAGAAAGCT CACAAGAAGG CTGTAATCAA TAAGTTAGAA 2520

GAGGAAATGG CAAAGCGGCA ACCATTGAAA AAGGGTGAAA AGGGTGTCAC AAAGTCTGCG 2580

TTGATAAAGA AAGAACGAGA GATTATGGCT CTCGAACAAG AACGCGATAC GATGAGTAAA 2640

AGGATTGCCG CTCTGTTTTA CGAAAATGAT AAGCAAGCCG AGCATTTCAA CATTGCGATT 2700

CAAGATATGC AGACTACACA AGACGCTTTA CGAGATGAGC TTAAAGAATG CAAAGAGGAG 2760

TTAGCCAACA GAAATGTGAA TACTCGATAT GAAGATAAAC GATCATTGGA TTCTCGAGAA 2820

GGGATTCCAT CTTCGTTAAG CAATCAACAT ATTCAAATGG AAGGCTGGCT ATCGCTCAGA 2880

GATAATACAA AGAAGTCACG GAAACCGAAG TGGACCAACT GCTTTGTTGC GCTTAACGAG 2940

TATTCATTCA CAATCTATGT CGACGAAAAA GCCGTGTCTG TGATTCTTTT GATTGAAGCC 3000

GGAGCTATGG CTCATGTTCG ACACGTCACT GCTGCTGATT TGAGAAATGT CGACGATAGT 3060

CAGTTACCTA AGATATTCCA TATCATGTAT GACGACGCGT CGTGCAATTC GTCACGACAC 3120

GCCTCCAATT CAGATCTCTC AATGATTGAA TCGTTCCGAG AAGAAAGTTG GAAGCGGCAC 3180

GATTTCCAAG AGCTCTCCTA TCACATACGC ACGTTTTGTG ATGTCTGCAA TAAGAAATTG 3240

TCTGACATTA TCCGACCGAC GCCTGCATTC GAATGCAAAA ATTGCCATTT CAAGACACAC 3300

AAGGATCATG TTGCTCAAGG ATCGTTGCCA ATGTGTCGAT ACAACACGGG TCTCTCTCGT 3360

GAACTCGTTC TTATGGCTCC ACAAACGGAT AACTGCAATA AATGGGTATC ACAACTTCGA 3420

CGCTTCATTG AATCATCACG ATCAGCGGCA GTTTCAGTGT CTCGTGTCTC TTCACGACGA 3480

CATGCTCCAG GATCGTCAAA CTCTTCCACA ATCTATCAAT AG 3522

Domain Profile

S: 1     feilkviGrGafgevavvrekatekvyamkilkklellkraesaffreerdvlvkadsew  60

         f+ lkviGrGafgev++vr+++t++vyamk+l+k +++kra+saff+eerd++++a+sew

Q: 68    FRQLKVIGRGAFGEVHLVRHTRTNTVYAMKMLNKDDMIKRADSAFFWEERDIMAHANSEW  127

         7889********************************************************

S: 61    ivqlqyafqdekylylvmeylvGGdlltllskyedklpeelarfylaelvlaldsvhelg  120

         iv+lqyafqd ++ly+vmey++GGdl++l+++ye  ++e+++rfy+ae+v al ++h++g

Q: 128   IVRLQYAFQDPRHLYMVMEYMPGGDLVNLMTSYE--VSEKWTRFYTAEIVEALAALHSMG  185

         **********************************..9***********************

S: 121   yvhrdikpdnilldksGhikladfGsclklnedgtvkssvavGtpdyispevlkaleegk  180

         y+hrd+kpdn+l++ sGhikladfG+c+k+n++g+v++s+avGtpdyispevl++++ ++

Q: 186   YIHRDVKPDNMLISISGHIKLADFGTCVKMNANGVVRCSTAVGTPDYISPEVLRNQG-QD  244

         ********************************************************9.99

S: 181   geygkecdwwslGvilyellvgetpfyaeslvetyskilnhkeslkfpedekvseeakdl  240

         +e+gke+dwws+Gv++ye+lvgetpfyae+lv+ty++i+nhk+slkfp++  +s++akd+

Q: 245   AEFGKEVDWWSVGVFIYEMLVGETPFYAEALVSTYTNIMNHKTSLKFPDEPLISTQAKDI  304

         ************************************************************

S: 241   ikklltsreerlgregiedlkkhpff  266

         ikk+l+++ +rlgr++++d+++h+ff

Q: 305   IKKFLSAAPDRLGRNSVDDIRNHKFF  330

         *************************9

Domain Sequence
(FASTA)

FRQLKVIGRG AFGEVHLVRH TRTNTVYAMK MLNKDDMIKR ADSAFFWEER DIMAHANSEW 60

IVRLQYAFQD PRHLYMVMEY MPGGDLVNLM TSYEVSEKWT RFYTAEIVEA LAALHSMGYI 120

HRDVKPDNML ISISGHIKLA DFGTCVKMNA NGVVRCSTAV GTPDYISPEV LRNQGQDAEF 180

GKEVDWWSVG VFIYEMLVGE TPFYAEALVS TYTNIMNHKT SLKFPDEPLI STQAKDIIKK 240

FLSAAPDRLG RNSVDDIRNH KFF 263

Keyword

ATP-binding; Coiled coil; Complete proteome; Cytoplasm; Cytoskeleton; Developmental protein; Differentiation; Kinase; Magnesium; Metal-binding; Nucleotide-binding; Oogenesis; Reference proteome; Serine/threonine-protein kinase; Transferase; Zinc; Zinc-finger.

Sequence Source

Ensembl

Orthology

Ortholog group

Ailuropoda melanoleuca"; ?>Anolis carolinensis"; ?>Callithrix jacchus"; ?>Canis familiaris"; ?>Cavia porcellus"; ?>Ciona intestinalis"; ?>Ciona savignyi"; ?>Danio rerio"; ?>Drosophila melanogaster"; ?>Equus caballus"; ?>Felis catus"; ?>Gadus morhua"; ?>Gallus gallus"; ?>Gasterosteus aculeatus"; ?>Gorilla gorilla"; ?>Homo sapiens"; ?>Latimeria chalumnae"; ?>Loxodonta africana"; ?>Meleagris gallopavo"; ?>Monodelphis domestica"; ?>Mus musculus"; ?>Mustela putorius furo"; ?>Myotis lucifugus"; ?>Oreochromis niloticus"; ?>Ornithorhynchus anatinus"; ?>Oryctolagus cuniculus"; ?>Oryzias latipes"; ?>Otolemur garnettii"; ?>Pan troglodytes"; ?>Pelodiscus sinensis"; ?>Petromyzon marinus"; ?>Rattus norvegicus"; ?>Sarcophilus harrisii"; ?>Sus scrofa"; ?>Taeniopygia guttata"; ?>Takifugu rubripes"; ?>Tetraodon nigroviridis"; ?>Xenopus tropicalis"; ?>Xiphophorus maculatus"; ?>

Gene Ontology

GO:0005913	; C:cell-cell adherens junction
GO:0032154	; C:cleavage furrow
GO:0043292	; C:contractile fiber
GO:0005856	; C:cytoskeleton
GO:0005634	; C:nucleus
GO:0005524	; F:ATP binding
GO:0046872	; F:metal ion binding
GO:0005543	; F:phospholipid binding
GO:0004674	; F:protein serine/threonine kinase activity
GO:0006915	; P:apoptotic process
GO:0030154	; P:cell differentiation
GO:0016477	; P:cell migration
GO:0040002	; P:collagen and cuticulin-based cuticle development
GO:0008340	; P:determination of adult lifespan
GO:0009792	; P:embryo development ending in birth or egg hatching
GO:0010172	; P:embryonic body morphogenesis
GO:0001703	; P:gastrulation with mouth forming first
GO:0040007	; P:growth
GO:0002119	; P:nematode larval development
GO:0048477	; P:oogenesis
GO:0008360	; P:regulation of cell shape
GO:0000003	; P:reproduction
GO:0007266	; P:Rho protein signal transduction

KEGG

cel:CELE_C10H11.9

;

InterPros

IPR000961	; AGC-kinase_C.
IPR011009	; Kinase-like_dom.
IPR011993	; PH_like_dom.
IPR017892	; Pkinase_C.
IPR001849	; Pleckstrin_homology.
IPR002219	; Prot_Kinase_C-like_PE/DAG-bd.
IPR000719	; Prot_kinase_cat_dom.
IPR017441	; Protein_kinase_ATP_BS.
IPR002290	; Ser/Thr_dual-sp_kinase_dom.
IPR008271	; Ser/Thr_kinase_AS.

Pfam

PF00069	; Pkinase; 1.
PF00433	; Pkinase_C; 1.

SMARTs

SM00109	; C1; 1.
SM00233	; PH; 1.
SM00133	; S_TK_X; 1.
SM00220	; S_TKc; 1.

Prosites

PS51285	; AGC_KINASE_CTER; 1.
PS50003	; PH_DOMAIN; FALSE_NEG.
PS00107	; PROTEIN_KINASE_ATP; 1.
PS50011	; PROTEIN_KINASE_DOM; 1.
PS00108	; PROTEIN_KINASE_ST; 1.
PS00479	; ZF_DAG_PE_1; FALSE_NEG.
PS50081	; ZF_DAG_PE_2; 1.

Prints

Created Date

20-Feb-2013