EKS-CAE-00235

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EKS-CAE-00235

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
Other/PLK	406.3	7.9E-122	38	289	252

Status

Reviewed

Ensembl Protein

C14B9.4a.1

UniProt Accession

P34331; O61662; O76763;

Protein Name

Serine/threonine-protein kinase plk-1

Protein Synonyms/Alias

Polo-like kinase 1;

Gene Name

plk-1

Gene Synonyms/Alias

plk-1; plc1; C14B9.4;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
C14B9.4	C14B9.4a.2	C14B9.4a.2
C14B9.4	C14B9.4a.1	C14B9.4a.1
C14B9.4	C14B9.4b	C14B9.4b

Organism

Caenorhabditis elegans

Functional Description

Required for oocyte nuclear envelope breakdown beforeentry of oocyte into spermatheca. In meiotic cells, required for spindle dynamics and probably for spindle attachment to the chromosomes. Zygotic role in the development of the germline and nerve cord. In mitotic cells, plays a role in spindle organization and centrosome maturation. Involved in asymmetric nuclear localization of cdc-25.1 during embryogenesis which affects cell division timing.

Protein Length

648

Protein Sequence
(FASTA)

MNRLPNIAKP PQKSNQRKEK APPEVPALIA DKDRGTYYEK GRFLGKGGFA HCYELTNRAT 60

REVVAGKVVP KSMLVKQYQR DKMTQEVQIH RELGHINIVK LFNFFEDNLN VYITLELCAR 120

RSLMELHKRR KAVTEPEARY FTHQIVDGVL YLHDLNIIHR DMKLGNLFLN DDLVVKIGDF 180

GLATTVNGDE RKKTLCGTPN YIAPEVLNKA GHSFEVDIWA VGCILYILLF GQPPFESKSL 240

EETYSRIRHN NYTIPSIATQ PAASLIRKML DPEPTRRPTA KQVQRDGFFK SGFMPTRLPV 300

SCLTMVPKFG GHETSMMEEN VAPRGVDARS QRPLNGRAGL SALPQHIVSN NADRERAQQQ 360

AAEATFREPE DAYLSQLFHQ VAVLLEQRIP GLEEEEAALD GYQSPECLPV FWISKWVDYS 420

DKYGIGYQLC DNSVGVLFND NSRIMLDQAG NELTYIEKSN KEHYFSMHSG EMPGLLNKKV 480

TLLKYFRSYM NDHLVKAGEG SEQRAGDDLA RLPTLRVWFR TKSAIVLHLS NGTVQINFFN 540

DHVKMMMCPL MQAVTFIDQN KRMLTYKLNN LQRNGCPEKF LHRLKYAKTM IERLMSDANV 600

VSQNPARQPD MPRSMAAARS ASAGSRGPNQ AASHLPQSAS GSNIHPRR 648

Nucleotide Sequence
(FASTA)

ATGAATCGAC TTCCCAACAT TGCAAAACCC CCACAAAAGT CCAACCAAAG AAAGGAGAAG 60

GCTCCACCAG AGGTTCCCGC TCTCATCGCC GACAAGGATC GTGGGACCTA TTATGAGAAA 120

GGACGATTTT TGGGCAAGGG AGGATTCGCT CACTGTTATG AGCTTACGAA TCGAGCAACA 180

CGCGAGGTTG TCGCTGGAAA AGTGGTTCCG AAGTCGATGC TCGTCAAGCA ATACCAGAGA 240

GATAAGATGA CACAAGAGGT GCAGATTCAT CGAGAGCTTG GACACATCAA TATCGTGAAG 300

TTATTCAACT TCTTCGAGGA CAATCTCAAC GTGTATATCA CTTTGGAGTT ATGTGCAAGA 360

CGGTCGCTGA TGGAGTTGCA CAAAAGAAGA AAGGCGGTCA CCGAACCTGA AGCTCGTTAT 420

TTCACTCATC AAATTGTGGA TGGCGTGCTG TATTTGCATG ATCTGAACAT CATTCATCGT 480

GATATGAAGC TTGGAAATCT TTTCCTCAAT GATGATCTAG TTGTCAAAAT TGGAGATTTC 540

GGATTAGCAA CAACGGTCAA CGGTGATGAG AGAAAGAAAA CGTTGTGTGG AACGCCAAAC 600

TACATAGCTC CGGAGGTTTT GAACAAAGCT GGACACTCAT TTGAAGTTGA CATTTGGGCA 660

GTTGGATGCA TTCTGTACAT TCTTCTTTTC GGACAACCAC CATTCGAGTC TAAATCGCTT 720

GAAGAGACAT ACTCACGCAT TCGCCACAAC AACTACACCA TTCCGTCGAT CGCAACACAA 780

CCAGCAGCAA GCCTAATCCG CAAGATGCTT GATCCTGAGC CAACACGTCG TCCGACTGCC 840

AAGCAGGTTC AACGCGATGG CTTCTTCAAG TCAGGATTCA TGCCGACTAG ACTTCCAGTC 900

TCCTGCCTCA CTATGGTGCC AAAGTTCGGA GGACACGAAA CTTCGATGAT GGAGGAAAAT 960

GTTGCTCCAA GAGGGGTTGA TGCTCGTTCA CAACGCCCAT TGAACGGAAG AGCTGGATTG 1020

TCTGCCCTCC CACAACACAT CGTGTCGAAC AATGCCGATC GTGAGCGTGC TCAACAACAA 1080

GCTGCAGAGG CGACTTTCCG GGAGCCCGAG GATGCATACC TTTCACAACT TTTCCACCAA 1140

GTTGCTGTGC TCTTAGAGCA GCGTATTCCA GGGCTGGAAG AAGAAGAAGC TGCTCTTGAT 1200

GGTTATCAAT CACCAGAGTG CCTCCCGGTT TTCTGGATTT CGAAATGGGT TGACTATTCG 1260

GACAAGTATG GCATCGGATA TCAACTATGT GACAATTCCG TCGGAGTTCT CTTCAATGAC 1320

AATTCTCGTA TCATGCTTGA TCAAGCTGGA AACGAGTTGA CCTATATTGA GAAGAGCAAC 1380

AAAGAGCACT ACTTCTCGAT GCACTCAGGT GAAATGCCTG GATTGCTCAA CAAGAAAGTC 1440

ACATTGCTTA AGTATTTTCG ATCCTACATG AATGACCATT TGGTGAAAGC TGGAGAAGGA 1500

TCTGAACAAA GAGCTGGTGA TGACTTGGCT CGTTTACCAA CGCTTCGAGT TTGGTTCAGA 1560

ACCAAATCAG CAATTGTTTT GCACCTTTCC AATGGAACTG TTCAGATAAA TTTCTTCAAT 1620

GATCATGTCA AGATGATGAT GTGCCCATTA ATGCAAGCAG TTACATTCAT CGATCAAAAC 1680

AAGCGTATGC TCACCTACAA GCTGAACAAT CTTCAGCGCA ATGGATGTCC AGAGAAATTC 1740

CTTCACCGAC TGAAGTATGC GAAGACAATG ATCGAAAGGT TGATGAGCGA TGCTAACGTT 1800

GTATCCCAGA ATCCAGCTCG TCAACCGGAT ATGCCACGGT CAATGGCAGC TGCTCGTTCA 1860

GCTTCGGCAG GATCACGAGG ACCAAATCAA GCTGCATCGC ACCTTCCCCA GTCGGCAAGT 1920

GGATCCAATA TTCACCCACG TCGATAG 1947

Domain Profile

S: 1     yergkllGkGgfakcyelkdletkevfavkvvpksllakekqrekvekeieihrslkhkn  60

         ye+g++lGkGgfa+cyel++  t+ev+a kvvpks+l+k++qr+k+++e++ihr+l h n

Q: 38    YEKGRFLGKGGFAHCYELTNRATREVVAGKVVPKSMLVKQYQRDKMTQEVQIHRELGHIN  97

         99**********************************************************

S: 61    ivklyeffedkenvylvlelcsrrslaellkrrkaltepevryylrqivsglkylhekri  120

         ivkl++ffed+ nvy++lelc+rrsl+el+krrka+tepe+ry+++qiv+g++ylh+ +i

Q: 98    IVKLFNFFEDNLNVYITLELCARRSLMELHKRRKAVTEPEARYFTHQIVDGVLYLHDLNI  157

         ************************************************************

S: 121   lhrdlklgnlflneelevkigdfGlatrlekeeerkktlcGtPnyiaPevlskkghslev  180

         +hrd+klgnlfln++l vkigdfGlat+++  +erkktlcGtPnyiaPevl+k+ghs+ev

Q: 158   IHRDMKLGNLFLNDDLVVKIGDFGLATTVN-GDERKKTLCGTPNYIAPEVLNKAGHSFEV  216

         *****************************9.99***************************

S: 181   diWslGcilytllvGkPPfetkelketyekikkaeyslPsslskeaksliakllkkePee  240

         diW++Gcily+ll+G+PPfe+k+l+ety++i++++y++Ps ++++a+sli+k+l++eP++

Q: 217   DIWAVGCILYILLFGQPPFESKSLEETYSRIRHNNYTIPSIATQPAASLIRKMLDPEPTR  276

         ************************************************************

S: 241   rpsleevlkdefl  253

         rp++++v +d f+

Q: 277   RPTAKQVQRDGFF  289

         *******999997

Domain Sequence
(FASTA)

YEKGRFLGKG GFAHCYELTN RATREVVAGK VVPKSMLVKQ YQRDKMTQEV QIHRELGHIN 60

IVKLFNFFED NLNVYITLEL CARRSLMELH KRRKAVTEPE ARYFTHQIVD GVLYLHDLNI 120

IHRDMKLGNL FLNDDLVVKI GDFGLATTVN GDERKKTLCG TPNYIAPEVL NKAGHSFEVD 180

IWAVGCILYI LLFGQPPFES KSLEETYSRI RHNNYTIPSI ATQPAASLIR KMLDPEPTRR 240

PTAKQVQRDG FF 252

Keyword

Alternative splicing; ATP-binding; Chromosome; Complete proteome; Cytoplasm; Cytoskeleton; Kinase; Nucleotide-binding; Nucleus; Reference proteome; Repeat; Serine/threonine-protein kinase; Transferase.

Sequence Source

Ensembl

Orthology

Ortholog group

Ailuropoda melanoleuca"; ?>Anolis carolinensis"; ?>Bos taurus"; ?>Callithrix jacchus"; ?>Canis familiaris"; ?>Cavia porcellus"; ?>Choloepus hoffmanni"; ?>Ciona intestinalis"; ?>Danio rerio"; ?>Dasypus novemcinctus"; ?>Dipodomys ordii"; ?>Echinops telfairi"; ?>Equus caballus"; ?>Felis catus"; ?>Gallus gallus"; ?>Gasterosteus aculeatus"; ?>Gorilla gorilla"; ?>Homo sapiens"; ?>Ictidomys tridecemlineatus"; ?>Latimeria chalumnae"; ?>Loxodonta africana"; ?>Macaca mulatta"; ?>Meleagris gallopavo"; ?>Microcebus murinus"; ?>Monodelphis domestica"; ?>Mus musculus"; ?>Mustela putorius furo"; ?>Myotis lucifugus"; ?>Nomascus leucogenys"; ?>Oreochromis niloticus"; ?>Ornithorhynchus anatinus"; ?>Oryctolagus cuniculus"; ?>Oryzias latipes"; ?>Otolemur garnettii"; ?>Pan troglodytes"; ?>Pelodiscus sinensis"; ?>Pongo abelii"; ?>Procavia capensis"; ?>Rattus norvegicus"; ?>Sarcophilus harrisii"; ?>Sus scrofa"; ?>Taeniopygia guttata"; ?>Takifugu rubripes"; ?>Tetraodon nigroviridis"; ?>Tupaia belangeri"; ?>Tursiops truncatus"; ?>Vicugna pacos"; ?>Xenopus tropicalis"; ?>Xiphophorus maculatus"; ?>

Gene Ontology

GO:0005813	; C:centrosome
GO:0000793	; C:condensed chromosome
GO:0005634	; C:nucleus
GO:0051233	; C:spindle midzone
GO:0005524	; F:ATP binding
GO:0004674	; F:protein serine/threonine kinase activity
GO:0006915	; P:apoptotic process
GO:0007098	; P:centrosome cycle
GO:0008340	; P:determination of adult lifespan
GO:0009792	; P:embryo development ending in birth or egg hatching
GO:0007147	; P:female meiosis II
GO:0040035	; P:hermaphrodite genitalia development
GO:0045132	; P:meiotic chromosome segregation
GO:0007067	; P:mitosis
GO:0007077	; P:mitotic nuclear envelope disassembly
GO:0007052	; P:mitotic spindle organization
GO:0002119	; P:nematode larval development
GO:0048477	; P:oogenesis
GO:0040038	; P:polar body extrusion after meiotic divisions
GO:0051726	; P:regulation of cell cycle

KEGG

cel:CELE_C14B9.4

;

InterPros

IPR011009	; Kinase-like_dom.
IPR000959	; POLO_box_duplicated_dom.
IPR000719	; Prot_kinase_cat_dom.
IPR017441	; Protein_kinase_ATP_BS.
IPR002290	; Ser/Thr_dual-sp_kinase_dom.
IPR008271	; Ser/Thr_kinase_AS.

Pfam

PF00069	; Pkinase; 1.
PF00659	; POLO_box; 2.

SMARTs

SM00220

; S_TKc; 1.

Prosites

PS50078	; POLO_BOX; 2.
PS00107	; PROTEIN_KINASE_ATP; 1.
PS50011	; PROTEIN_KINASE_DOM; 1.
PS00108	; PROTEIN_KINASE_ST; 1.

Prints

Created Date

20-Feb-2013