EKS-CAE-00246

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EKS-CAE-00246

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
Other/IRE	429.7	6.2E-129	176	436	261

Status

Reviewed

Ensembl Protein

C41C4.4b

UniProt Accession

Q09499; Q8WRG0;

Protein Name

Endoribonuclease

Protein Synonyms/Alias

Inositol-requiring protein 2;

Gene Name

ire-1

Gene Synonyms/Alias

ire-1; C41C4.4;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
C41C4.4	C41C4.4b	C41C4.4b
C41C4.4	C41C4.4a	C41C4.4a

Organism

Caenorhabditis elegans

Functional Description

Senses unfolded proteins in the lumen of the endoplasmicreticulum via its N-terminal domain which leads to enzyme auto- activation. The active endoribonuclease domain splices xbp-1 precursor mRNA to produce the mature form which then induces transcription of UPR target genes. Unfolded protein response (UPR) transcripional activation by ire-1, as well as translational attenuation by pek-1 in a complementary pathway, maintains ER homeostasis. Ire-1 and pek-1 are redundant genes that control a pathway essential for larval development and survival.

Protein Length

625

Protein Sequence
(FASTA)

MEKEEYLPPR RPIIRNIPPS ITHKTSDGEY LLLGYHDRPM MTMATIIPTR YPVPGPHKAI 60

GSTIERPPPQ LLGPVEPQKH EDTSFILLLL NNHPIPFYAT LVTMFALLLT VIWQCGRQWD 120

QQKSTSRMDS FEIVNNPGES RSAQTSKQSN RGSFGWANRK IEIPEGWMAV GSKLMYSPSD 180

ILGTGCEGTV VYRGTFDGRE VAVKRVVSEF VKFAHREADL LRESDTHPHV IRYFCMESDS 240

QFRYLALELC IASLNDYVEQ KEVQQNVTIA LRDIMKQATD GLAHLHASKI VHRDMKPQNV 300

LITMASQRGE MRAVISDFGL CKRVQPGKNS ISRGIASGLA GTDGWIAPEV LISASTSYPV 360

DIFSLGCIFY YVLTSGTHPF GKSLHRQANI VNGEYTLNKL ADLDDWSLAD DLISSMLNVE 420

PLHRLTADAV LNHPFFWTSE KRLAYFSDVS DRVEKEEDNS PVVRRIETDA RIVVCGGWRE 480

KICDALKEDL RKFRTYKSFS VRDLLRAMRN KKHHYRELPE DVRQSLGDIP DQFLHYFTSR 540

FPRLLLHVYK ATEYCSGEAV FKRYYSDDVR ARMYPIVEEE ERVRKKIKEE MANEVWARAP 600

KPVEQRTPLK LDKRNIKKKS NPNTD 625

Nucleotide Sequence
(FASTA)

ATGGAAAAAG AAGAGTACTT GCCACCACGG AGACCAATAA TTCGAAATAT TCCTCCATCA 60

ATTACTCATA AAACTTCGGA TGGAGAATAC TTATTATTAG GATATCATGA TCGACCAATG 120

ATGACGATGG CAACGATTAT TCCGACAAGA TATCCAGTTC CTGGACCTCA TAAGGCAATT 180

GGATCTACAA TTGAACGGCC TCCACCACAA CTTCTGGGAC CTGTTGAGCC ACAGAAGCAT 240

GAGGACACAT CTTTCATTCT GCTTCTACTC AATAATCATC CTATTCCATT CTATGCTACA 300

CTGGTTACGA TGTTTGCATT ACTATTAACT GTAATTTGGC AATGTGGAAG ACAATGGGAT 360

CAACAGAAGT CGACGTCAAG AATGGATTCC TTCGAAATTG TAAACAATCC CGGAGAATCT 420

AGAAGTGCTC AGACATCAAA ACAATCGAAT CGTGGAAGCT TTGGATGGGC GAATAGAAAA 480

ATTGAAATTC CCGAGGGTTG GATGGCAGTT GGTAGCAAGT TGATGTATAG TCCATCAGAT 540

ATTCTTGGAA CAGGATGTGA AGGAACTGTG GTTTATAGAG GAACTTTTGA TGGAAGAGAA 600

GTTGCTGTAA AGAGAGTAGT TTCAGAATTC GTGAAGTTTG CTCACAGAGA AGCGGATTTA 660

TTACGAGAAA GTGATACACA TCCACACGTT ATCAGATACT TTTGTATGGA ATCAGACAGT 720

CAATTTCGAT ATTTGGCACT CGAACTTTGC ATAGCTTCTT TGAATGATTA TGTTGAGCAG 780

AAAGAGGTTC AACAAAATGT TACAATTGCT CTACGAGACA TTATGAAACA GGCAACCGAT 840

GGATTGGCAC ATCTTCATGC TTCAAAAATT GTGCATCGAG ATATGAAGCC ACAAAATGTT 900

CTAATTACAA TGGCTAGTCA GCGAGGAGAA ATGAGAGCAG TCATCTCAGA TTTTGGTCTT 960

TGTAAAAGGG TTCAACCTGG AAAGAATTCA ATTTCAAGAG GTATTGCGTC TGGATTAGCA 1020

GGAACCGATG GCTGGATTGC TCCAGAAGTT CTTATTTCTG CAAGTACTTC TTATCCAGTT 1080

GATATCTTCT CACTTGGATG TATTTTCTAT TATGTGTTAA CCTCGGGTAC TCATCCTTTT 1140

GGAAAATCAT TGCACAGACA AGCAAATATT GTAAATGGAG AATACACATT GAATAAATTG 1200

GCAGATTTGG ATGATTGGTC GTTGGCTGAT GACTTGATCT CTTCAATGCT GAACGTGGAG 1260

CCATTGCACC GATTGACCGC TGATGCCGTC TTGAATCATC CATTTTTCTG GACATCTGAA 1320

AAGCGATTAG CATATTTTTC GGATGTATCT GATCGTGTAG AGAAAGAAGA AGACAATAGT 1380

CCAGTTGTGA GAAGAATAGA AACAGATGCT CGAATAGTTG TATGCGGTGG ATGGAGGGAG 1440

AAGATTTGTG ATGCTTTGAA GGAAGATTTA AGAAAGTTCA GAACATACAA ATCATTTTCA 1500

GTTCGAGATT TGCTAAGAGC AATGCGTAAC AAGAAGCATC ACTATAGAGA ATTACCAGAA 1560

GATGTCAGAC AATCTCTTGG AGACATTCCA GATCAGTTCT TGCATTATTT TACATCACGC 1620

TTCCCTCGGC TATTACTTCA TGTTTACAAA GCAACCGAGT ATTGTTCCGG AGAGGCTGTC 1680

TTCAAAAGAT ACTATTCCGA TGATGTTCGT GCTCGAATGT ATCCAATTGT GGAAGAAGAA 1740

GAGAGAGTAA GAAAGAAGAT TAAAGAAGAA ATGGCAAATG AGGTGTGGGC ACGAGCACCA 1800

AAACCTGTCG AGCAACGAAC TCCTCTAAAA TTGGATAAGA GGAATATCAA AAAGAAATCA 1860

AATCCGAACA CCGATTAA 1878

Domain Profile

S: 1     fspkdvlGkGaeGtvvfrGsfegrevavkrllsefvdlakrevkllresdehpnviryfc  60

         +sp+d+lG+G+eGtvv+rG+f+grevavkr++sefv++a+re++llresd+hp+viryfc

Q: 176   YSPSDILGTGCEGTVVYRGTFDGREVAVKRVVSEFVKFAHREADLLRESDTHPHVIRYFC  235

         689*********************************************************

S: 61    tekdrqfryialelcaaslqdyvedkdlelqkelepisllkqiasGlahlhslkivhrdl  120

         +e+d+qfry+alelc asl+dyve+k++++++++++++++kq+++Glahlh++kivhrd+

Q: 236   MESDSQFRYLALELCIASLNDYVEQKEVQQNVTIALRDIMKQATDGLAHLHASKIVHRDM  295

         ************************************************************

S: 121   kpqnilislpdakgklrvlisdfGlckkldvGkssfsr..rsgvsGteGwiapelleelt  178

         kpqn+li++++++g++r++isdfGlck+++ Gk+s+sr  +sg++Gt+Gwiape+l

Q: 296   KPQNVLITMASQRGEMRAVISDFGLCKRVQPGKNSISRgiASGLAGTDGWIAPEVLI---  352

         **************************************999***************9...

S: 179   kerltsavdifslGcvfyyvlskGkhpfGdslkrqanilsgelsldklkeekdeeviaed  238

         +++++++vdifslGc+fyyvl++G+hpfG+sl+rqani++ge++l+kl++++d++ +a+d

Q: 353   SASTSYPVDIFSLGCIFYYVLTSGTHPFGKSLHRQANIVNGEYTLNKLADLDDWS-LADD  411

         99*****************************************************.9***

S: 239   liskmidkdplkrpsakkvlkhplf  263

         lis+m++++pl+r++a++vl+hp+f

Q: 412   LISSMLNVEPLHRLTADAVLNHPFF  436

         ************************9

Domain Sequence
(FASTA)

YSPSDILGTG CEGTVVYRGT FDGREVAVKR VVSEFVKFAH READLLRESD THPHVIRYFC 60

MESDSQFRYL ALELCIASLN DYVEQKEVQQ NVTIALRDIM KQATDGLAHL HASKIVHRDM 120

KPQNVLITMA SQRGEMRAVI SDFGLCKRVQ PGKNSISRGI ASGLAGTDGW IAPEVLISAS 180

TSYPVDIFSL GCIFYYVLTS GTHPFGKSLH RQANIVNGEY TLNKLADLDD WSLADDLISS 240

MLNVEPLHRL TADAVLNHPF F 261

Keyword

ATP-binding; Complete proteome; Endoplasmic reticulum; Glycoprotein; Hydrolase; Kinase; Membrane; Multifunctional enzyme; Nucleotide-binding; Phosphoprotein; Reference proteome; Serine/threonine-protein kinase; Signal; Transferase; Transmembrane; Transmembrane helix; Unfolded protein response.

Sequence Source

Ensembl

Orthology

Ortholog group

Ailuropoda melanoleuca"; ?>Anolis carolinensis"; ?>Bos taurus"; ?>Callithrix jacchus"; ?>Canis familiaris"; ?>Cavia porcellus"; ?>Ciona intestinalis"; ?>Ciona savignyi"; ?>Danio rerio"; ?>Drosophila melanogaster"; ?>Echinops telfairi"; ?>Equus caballus"; ?>Felis catus"; ?>Gadus morhua"; ?>Gallus gallus"; ?>Gasterosteus aculeatus"; ?>Gorilla gorilla"; ?>Homo sapiens"; ?>Ictidomys tridecemlineatus"; ?>Latimeria chalumnae"; ?>Loxodonta africana"; ?>Macaca mulatta"; ?>Meleagris gallopavo"; ?>Microcebus murinus"; ?>Monodelphis domestica"; ?>Mus musculus"; ?>Mustela putorius furo"; ?>Myotis lucifugus"; ?>Nomascus leucogenys"; ?>Ochotona princeps"; ?>Oreochromis niloticus"; ?>Ornithorhynchus anatinus"; ?>Oryctolagus cuniculus"; ?>Oryzias latipes"; ?>Otolemur garnettii"; ?>Pan troglodytes"; ?>Pelodiscus sinensis"; ?>Pongo abelii"; ?>Rattus norvegicus"; ?>Sarcophilus harrisii"; ?>Sus scrofa"; ?>Taeniopygia guttata"; ?>Takifugu rubripes"; ?>Tetraodon nigroviridis"; ?>Tupaia belangeri"; ?>Xenopus tropicalis"; ?>Xiphophorus maculatus"; ?>Saccharomyces cerevisiae"; ?>Schizosaccharomyces pombe"; ?>

Gene Ontology

GO:0005789	; C:endoplasmic reticulum membrane
GO:0016021	; C:integral to membrane
GO:0005524	; F:ATP binding
GO:0016891	; F:endoribonuclease activity, producing 5'-phosphomonoesters
GO:0004674	; F:protein serine/threonine kinase activity
GO:0030968	; P:endoplasmic reticulum unfolded protein response
GO:0019915	; P:lipid storage
GO:0006397	; P:mRNA processing
GO:0002119	; P:nematode larval development
GO:0040017	; P:positive regulation of locomotion
GO:0000003	; P:reproduction

KEGG

cel:CELE_C41C4.4

;

InterPros

IPR010513	; KEN_RNase_activator.
IPR011009	; Kinase-like_dom.
IPR018391	; PQQ_beta_propeller_repeat.
IPR002372	; PQQ_repeat.
IPR000719	; Prot_kinase_cat_dom.
IPR006567	; PUG-dom.
IPR011047	; Quinonprotein_ADH-like.
IPR008271	; Ser/Thr_kinase_AS.

Pfam

PF00069	; Pkinase; 1.
PF01011	; PQQ; 2.
PF06479	; Ribonuc_2-5A; 1.

SMARTs

SM00564	; PQQ; 5.
SM00580	; PUG; 1.

Prosites

PS51392	; KEN; 1.
PS00107	; PROTEIN_KINASE_ATP; FALSE_NEG.
PS50011	; PROTEIN_KINASE_DOM; 1.
PS00108	; PROTEIN_KINASE_ST; 1.

Prints

Created Date

20-Feb-2013