EKS-AIM-00061

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EKS-AIM-00061

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
CMGC/CDK	371.5	2.6E-111	728	1021	294

Status

Reviewed

Ensembl Protein

ENSAMEP00000004181

UniProt Accession

D2H526;

Protein Name

Cyclin-dependent kinase 12

Protein Synonyms/Alias

Cell division protein kinase 12;

Gene Name

CDK12

Gene Synonyms/Alias

CDK12; PANDA_004952;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
ENSAMEG00000003944	ENSAMEP00000004181	ENSAMET00000004348

Organism

Ailuropoda melanoleuca

Functional Description

Cyclin-dependent kinase which displays CTD kinaseactivity and is required for RNA splicing. Has CTD kinase activity by hyperphosphorylating the C-terminal heptapeptide repeat domain (CTD) of the largest RNA polymerase II subunit RPB1, thereby acting as a key regulator of transcription elongation. Required for RNA splicing, possibly by phosphorylating SRSF1/SF2. Involved in regulation of MAP kinase activity, possibly leading to affect the response to estrogn inhibitors (By similarity).

Protein Length

1491

Protein Sequence
(FASTA)

MPNPERHGGK KDGSGGASGT LQPSSGGGSS NSRERHRLVS KHKRHKSKHS KDMGLVTPEA 60

APLGTIIKPL VEYDDISSDS DTFSDDMAFK LDRRENDERR GTDRSDRLHK HRHHQHRRSR 120

DLLKTKQTEK EKNQEVSSKS GSMKDRISGS SKRSNEENED YGKAQISKSS SNKESRSSKL 180

HKEKTRKERE LKSGHKDRSK SHRKRETPKS YKTVDSPKRR SRSPHRKWSD SPKQDDSPSG 240

ASYGQDYDLS PPRSHTSSNY DSYKKSPGST SRRQSISPPY KEPSAYQSST RSPSPYSRRQ 300

RSVSPYSRRR SSSYERSGSY SGRSPSPYGR RRSSSPFMSK RSLSRSPLPS RKSMKSRSRS 360

PAYSRHSSSH SKKKRSGSRS RHSSISPVRL PLNSSLGAEL SRKKKERAAA AAAAKMDGKE 420

SKGSPIFLPR KENSLVEAKD SGLESKKLTR GVKLEKSAPD TELVNIPHLN TEVKNSLDTG 480

KVKLDENSEK HPIKDLKAQG SRDSKPIALK EEIVTPKETE TSEKETPPPV PAVTSPPPPL 540

PTTSPPPQTP PLPPLPPLPA IPQQPPLPPP QPAFSHVLAS STSTLPPSTH PRTSTLSSQA 600

NSQPLAQVSV KTQVSVTAAI PHLKTSTLPP LPLPPLLPGD DDMDSPKETP PSKPVKKEKE 660

QRPRHLLTDL PLPPELPGGD PSPPDSPEPK AVTPPQQPYK KRPKICCPRY GERRQTESDW 720

GKRCVDKFDI IGIIGEGTYG QVYKAKDKDT GELVALKKVR LDNEKEGFPI TAIREIKILR 780

QLIHRSVVNM KEIVTDKQDA LDFKKDKGAF YLVFEYMDHD LMGLLESGLV HFSEDHIKSF 840

MKQLMEGLDY CHKKNFLHRD IKCSNILLNN SGQIKLADFG LARLYNSEES RPYTNKVITL 900

WYRPPELLLG EERYTPAIDV WSCGCILGEL FTKKPIFQAN LELAQLELIS RLCGSPCPAV 960

WPDVIKLPYF NTMKPKKQYR RRLREEFSFI PSAALDLLDH MLTLDPSKRC TAEQTLQSDF 1020

LKDVELSKMD PPDLPHWQDC HELWSKKRRR QRQSGVVIEE PPPSKASRKE TTSGTSAEPV 1080

KNSSPAPPQP ASGKVEPGTG DAIGLGDITQ QLNQSELAVL LNLLQSQTDL SIPQMAQLLN 1140

IHSNPEMQQQ LEALNQSISA LTEATSQQQD SEHMAPEESL KEAPPALVVQ PSAEQTTSEA 1200

SSTPADMQNM LAVLLSQLMK TQEPAGSLEE NNSDKNSGPQ GPRRTPTMPQ EEAAACPPHI 1260

LPPEKRPPEP PGPPPPPPPP PLIEGDLSSA PQELNPAVTA ALLQLLSQPE AEPPGHLPHE 1320

HQALRPMEYS TRPHPNRTYG NTDGPETGFS ATDTDERNSG PALTESLTQT LVKNRTFSGS 1380

VSHLGESSSY QGTGSVQFPG DQDLRFARVP LPLHSVVGQP FLKAEGSSNS VVHAETKLQN 1440

YGELGPGTTG ASSSGAGLNW GGSAQSSAYG KLYRGPTRVP PRGGRGRGVP Y 1491

Nucleotide Sequence
(FASTA)

ATGCCCAATC CAGAGAGACA TGGGGGTAAG AAGGACGGGA GTGGAGGAGC TTCTGGAACT 60

TTGCAGCCGT CATCGGGAGG TGGCAGCTCC AACAGCAGAG AGCGTCACCG CTTGGTGTCG 120

AAGCACAAGC GGCATAAATC CAAGCACTCC AAAGACATGG GGTTGGTGAC CCCCGAAGCA 180

GCACCTTTGG GTACAATTAT CAAACCTTTG GTGGAGTATG ATGACATCAG CTCTGATTCA 240

GATACCTTCT CTGACGACAT GGCCTTCAAA CTAGACAGGA GGGAGAATGA TGAACGTCGT 300

GGAACTGATC GGAGTGACCG CCTGCACAAA CATCGTCACC ACCAGCACAG ACGTTCCCGG 360

GACTTACTAA AAACTAAACA AACAGAAAAA GAAAAAAACC AGGAAGTCTC CAGCAAATCG 420

GGATCGATGA AGGACCGGAT ATCAGGAAGT TCAAAGCGTT CAAATGAGGA GAATGAGGAC 480

TATGGGAAGG CACAGATATC CAAAAGCAGC AGCAACAAGG AATCCAGGTC ATCCAAACTC 540

CATAAGGAGA AGACCCGGAA AGAACGTGAG TTGAAGTCTG GGCACAAAGA CCGGAGTAAA 600

AGTCATCGGA AAAGGGAAAC ACCCAAAAGC TACAAAACAG TCGACAGCCC CAAACGGAGA 660

TCCAGGAGTC CCCATAGGAA ATGGTCTGAC AGCCCCAAAC AAGATGATAG CCCCTCAGGA 720

GCTTCATATG GCCAAGATTA TGACCTTAGT CCCCCAAGAT CTCACACCTC TAGCAATTAT 780

GACTCCTACA AGAAGAGTCC TGGAAGTACC TCAAGAAGGC AGTCAATTAG CCCTCCTTAC 840

AAGGAGCCTT CGGCCTACCA GTCTAGCACC CGGTCACCCA GTCCCTACAG TAGACGGCAA 900

AGATCTGTGA GTCCCTATAG TCGGAGACGT TCTTCCAGCT ATGAAAGGAG TGGCTCTTAC 960

AGTGGGAGAT CACCCAGTCC CTATGGGCGA AGACGGTCCA GCAGCCCTTT CATGAGCAAA 1020

CGGTCTCTGA GTCGGAGTCC ACTCCCCAGT AGGAAATCCA TGAAGTCTAG AAGTAGAAGT 1080

CCCGCATATT CAAGACACTC ATCTTCTCAT AGTAAAAAGA AGCGATCCGG GTCACGCAGT 1140

CGACATTCCA GCATCTCACC TGTCAGGCTT CCATTGAACT CCAGCTTGGG AGCTGAACTC 1200

AGTAGGAAAA AGAAGGAAAG AGCAGCAGCT GCTGCTGCAG CAAAAATGGA TGGAAAGGAA 1260

TCCAAGGGTT CACCTATATT TTTGCCTAGA AAAGAGAACA GTTTAGTAGA GGCTAAGGAT 1320

TCAGGCTTGG AGTCTAAAAA GTTAACCAGA GGTGTAAAAT TGGAAAAATC TGCCCCAGAT 1380

ACTGAACTGG TGAATATACC ACATCTAAAT ACAGAAGTCA AAAATTCTTT AGATACAGGG 1440

AAAGTAAAGT TGGATGAGAA CTCTGAGAAG CATCCTATTA AAGATTTGAA AGCACAGGGA 1500

TCAAGAGACT CTAAACCCAT AGCGTTGAAA GAGGAGATTG TTACTCCAAA AGAGACAGAG 1560

ACATCAGAAA AGGAGACCCC TCCACCTGTC CCCGCAGTTA CTTCTCCTCC ACCTCCTTTA 1620

CCAACTACTA GCCCTCCACC TCAGACACCC CCTTTGCCAC CTTTGCCTCC ACTACCAGCT 1680

ATTCCACAGC AACCACCTCT GCCTCCTCCC CAGCCAGCAT TTAGTCATGT TCTTGCCTCT 1740

AGTACTTCAA CTTTGCCCCC TTCTACTCAC CCAAGGACAT CTACTTTGTC CTCTCAGGCA 1800

AATTCTCAGC CCCTTGCACA GGTTTCTGTG AAGACTCAAG TATCTGTAAC AGCTGCTATT 1860

CCACACCTAA AAACTTCAAC GTTGCCTCCT CTGCCCCTCC CACCCTTATT ACCAGGAGAC 1920

GATGACATGG ATAGTCCAAA AGAAACTCCA CCTTCAAAAC CTGTAAAGAA AGAGAAGGAA 1980

CAAAGACCAC GTCACTTACT CACGGACCTC CCTCTCCCTC CAGAACTTCC TGGTGGGGAT 2040

CCATCTCCTC CAGATTCTCC AGAACCAAAG GCAGTTACAC CACCTCAGCA ACCATATAAA 2100

AAGAGACCAA AAATTTGTTG TCCTCGTTAT GGAGAAAGAA GACAAACAGA AAGTGACTGG 2160

GGAAAACGCT GTGTAGACAA GTTTGACATT ATTGGGATTA TTGGAGAGGG AACTTATGGC 2220

CAAGTATATA AAGCCAAGGA CAAAGACACA GGAGAGCTAG TGGCCCTGAA GAAGGTGAGA 2280

CTGGACAATG AGAAGGAGGG CTTCCCAATT ACAGCCATTC GTGAGATCAA AATCCTTCGT 2340

CAACTAATCC ACCGAAGTGT TGTTAACATG AAGGAAATTG TTACAGATAA ACAAGATGCA 2400

CTGGATTTCA AGAAGGACAA AGGTGCCTTT TACCTTGTAT TTGAATATAT GGACCATGAC 2460

TTAATGGGAC TGCTAGAATC TGGTCTGGTG CACTTTTCTG AGGACCATAT CAAGTCATTC 2520

ATGAAACAGC TGATGGAAGG ACTGGATTAC TGTCACAAAA AGAATTTCCT GCATCGGGAT 2580

ATTAAGTGTT CTAACATTTT GCTGAACAAC AGTGGTCAGA TCAAACTAGC AGATTTTGGA 2640

CTTGCACGGC TCTATAACTC TGAAGAGAGT CGCCCTTATA CAAACAAAGT CATTACTCTG 2700

TGGTACCGAC CTCCAGAACT TCTGCTGGGA GAGGAGCGTT ATACACCAGC CATTGATGTT 2760

TGGAGCTGTG GATGCATCCT TGGGGAACTG TTCACAAAGA AGCCTATTTT TCAAGCCAAT 2820

CTGGAACTGG CTCAGCTAGA ACTGATCAGT CGACTCTGTG GTAGCCCTTG TCCAGCTGTG 2880

TGGCCTGATG TTATCAAGCT GCCCTACTTC AATACCATGA AACCGAAGAA GCAATATAGA 2940

AGGCGTCTGC GAGAAGAATT CTCTTTCATT CCTTCGGCAG CACTTGATTT ACTGGACCAC 3000

ATGCTGACAC TAGATCCTAG CAAACGTTGC ACAGCTGAAC AGACTTTACA GAGTGACTTC 3060

CTTAAAGATG TTGAGCTCAG CAAAATGGAT CCTCCAGACC TCCCCCACTG GCAGGATTGC 3120

CATGAATTAT GGAGTAAGAA GCGGCGACGA CAGCGACAGA GTGGTGTTGT GATAGAAGAA 3180

CCACCTCCAT CCAAAGCCTC TCGCAAAGAA ACTACCTCAG GGACAAGTGC TGAGCCTGTG 3240

AAGAACAGCA GCCCAGCACC ACCTCAGCCT GCTTCTGGCA AGGTGGAGCC TGGGACTGGG 3300

GATGCAATAG GCCTCGGTGA CATCACACAG CAGTTGAATC AAAGTGAATT GGCAGTGTTA 3360

CTGAATCTGC TGCAGAGCCA AACTGACCTG AGTATCCCTC AAATGGCTCA GCTGCTTAAC 3420

ATCCACTCCA ACCCAGAGAT GCAGCAGCAG CTGGAGGCCC TGAACCAATC CATCAGTGCC 3480

CTAACTGAAG CCACTTCCCA GCAGCAGGAC TCAGAGCACA TGGCCCCAGA GGAGTCTTTG 3540

AAGGAGGCAC CTCCTGCCCT AGTGGTCCAG CCTTCAGCAG AACAGACAAC CTCTGAAGCT 3600

TCAAGCACAC CAGCTGACAT GCAGAATATG TTGGCAGTTC TTTTGAGTCA GCTGATGAAA 3660

ACCCAGGAGC CAGCAGGCAG CCTGGAGGAA AACAACAGTG ACAAGAACAG TGGGCCACAG 3720

GGGCCCCGAA GAACTCCCAC AATGCCACAG GAGGAGGCAG CAGCATGTCC TCCTCACATT 3780

CTTCCACCAG AGAAGAGGCC CCCTGAGCCC CCCGGACCTC CACCGCCGCC ACCTCCACCC 3840

CCTCTGATTG AAGGCGATCT TTCCAGCGCC CCCCAGGAGT TGAACCCAGC CGTGACAGCC 3900

GCCTTGCTGC AACTTTTATC CCAGCCTGAA GCAGAGCCTC CTGGCCACCT GCCACATGAG 3960

CACCAGGCCT TGAGACCAAT GGAATACTCC ACCCGACCCC ATCCCAACAG GACTTACGGA 4020

AACACTGATG GGCCTGAAAC AGGGTTCAGT GCCACTGACA CTGATGAACG CAACTCTGGT 4080

CCAGCTTTGA CAGAATCCTT GACCCAGACC CTGGTGAAGA ACAGGACCTT CTCAGGCTCT 4140

GTGAGCCACC TTGGGGAGTC CAGCAGTTAC CAGGGCACAG GGTCAGTGCA GTTCCCAGGG 4200

GACCAGGACC TCCGTTTTGC CAGGGTCCCC TTACCATTAC ACTCAGTGGT CGGGCAACCA 4260

TTCCTGAAGG CTGAGGGAAG CAGCAACTCT GTGGTACATG CAGAGACCAA ATTGCAGAAC 4320

TATGGGGAGC TGGGGCCAGG AACCACTGGG GCCAGCAGTT CAGGAGCAGG CCTTAACTGG 4380

GGGGGCTCAG CTCAGTCTTC TGCTTATGGA AAACTCTATC GGGGGCCTAC AAGAGTCCCG 4440

CCAAGAGGGG GAAGAGGGAG AGGAGTTCCT TACTAA 4476

Domain Profile

S: 1     yeklekigeGtygkvykakdketgevvAlKkirlekekegvpitalrEisllkelkhkni  60

         ++ +  igeGtyg+vykakdk+tge+vAlKk+rl++ekeg+pita+rEi++l++l h+ +

Q: 728   FDIIGIIGEGTYGQVYKAKDKDTGELVALKKVRLDNEKEGFPITAIREIKILRQLIHRSV  787

         667889******************************************************

S: 61    vkLlevva.........kdkkklyLvfefleqdLkkllkkkkkkklsleevkslllqlle  111

         v+++e+v+         kdk  +yLvfe++++dL+ ll++    ++s++++ks+++ql+e

Q: 788   VNMKEIVTdkqdaldfkKDKGAFYLVFEYMDHDLMGLLESGLV-HFSEDHIKSFMKQLME  846

         ****************8899********************999.****************

S: 112   glaylHsnkilHRDlKpqNlLiskegklklaDfGlarafssplk.tytkevvTlwYraPe  170

         gl+y+H++++lHRD+K++N+L++++g++klaDfGlar ++s+++  yt++v+TlwYr+Pe

Q: 847   GLDYCHKKNFLHRDIKCSNILLNNSGQIKLADFGLARLYNSEESrPYTNKVITLWYRPPE  906

         ***************************************7766527**************

S: 171   lLlgakeYstavDiWsvgcilaelltrkplfqgkseidqlerifkllgtpsekvwpevsk  230

         lLlg+++Y+ a+D+Ws+gcil el+t+kp+fq++ e  qle i++l+g+p  +vwp+v k

Q: 907   LLLGEERYTPAIDVWSCGCILGELFTKKPIFQANLELAQLELISRLCGSPCPAVWPDVIK  966

         ************************************************************

S: 231   lpeykksfpkkkkk.klkekvkklkekaldllekllaldpkkRisakealqhkyf  284

         lp +++  pkk+++ +l+e+++ + ++aldll+++l+ldp+kR++a+++lq++++

Q: 967   LPYFNTMKPKKQYRrRLREEFSFIPSAALDLLDHMLTLDPSKRCTAEQTLQSDFL  1021

         *****7777777777******9999**************************9985

Domain Sequence
(FASTA)

FDIIGIIGEG TYGQVYKAKD KDTGELVALK KVRLDNEKEG FPITAIREIK ILRQLIHRSV 60

VNMKEIVTDK QDALDFKKDK GAFYLVFEYM DHDLMGLLES GLVHFSEDHI KSFMKQLMEG 120

LDYCHKKNFL HRDIKCSNIL LNNSGQIKLA DFGLARLYNS EESRPYTNKV ITLWYRPPEL 180

LLGEERYTPA IDVWSCGCIL GELFTKKPIF QANLELAQLE LISRLCGSPC PAVWPDVIKL 240

PYFNTMKPKK QYRRRLREEF SFIPSAALDL LDHMLTLDPS KRCTAEQTLQ SDFL 294

Keyword

Acetylation; ATP-binding; Complete proteome; Kinase; mRNA processing; mRNA splicing; Nucleotide-binding; Nucleus; Phosphoprotein; Reference proteome; Serine/threonine-protein kinase; Transferase.

Sequence Source

Ensembl

Orthology

Ortholog group

Anolis carolinensis"; ?>Bos taurus"; ?>Caenorhabditis elegans"; ?>Callithrix jacchus"; ?>Canis familiaris"; ?>Cavia porcellus"; ?>Ciona intestinalis"; ?>Ciona savignyi"; ?>Danio rerio"; ?>Equus caballus"; ?>Felis catus"; ?>Gadus morhua"; ?>Gallus gallus"; ?>Gorilla gorilla"; ?>Homo sapiens"; ?>Latimeria chalumnae"; ?>Loxodonta africana"; ?>Macaca mulatta"; ?>Monodelphis domestica"; ?>Mus musculus"; ?>Mustela putorius furo"; ?>Myotis lucifugus"; ?>Nomascus leucogenys"; ?>Oreochromis niloticus"; ?>Ornithorhynchus anatinus"; ?>Oryctolagus cuniculus"; ?>Oryzias latipes"; ?>Otolemur garnettii"; ?>Pan troglodytes"; ?>Pelodiscus sinensis"; ?>Pongo abelii"; ?>Rattus norvegicus"; ?>Sarcophilus harrisii"; ?>Sus scrofa"; ?>Tetraodon nigroviridis"; ?>Tursiops truncatus"; ?>Vicugna pacos"; ?>Xenopus tropicalis"; ?>Xiphophorus maculatus"; ?>Saccharomyces cerevisiae"; ?>Schizosaccharomyces pombe"; ?>

Gene Ontology

GO:0019908	; C:nuclear cyclin-dependent protein kinase holoenzyme complex
GO:0016607	; C:nuclear speck
GO:0005730	; C:nucleolus
GO:0005524	; F:ATP binding
GO:0004693	; F:cyclin-dependent protein kinase activity
GO:0008353	; F:RNA polymerase II carboxy-terminal domain kinase activity
GO:0007049	; P:cell cycle
GO:0006397	; P:mRNA processing
GO:0070816	; P:phosphorylation of RNA polymerase II C-terminal domain
GO:0046777	; P:protein autophosphorylation
GO:0043405	; P:regulation of MAP kinase activity
GO:0008380	; P:RNA splicing

KEGG

aml:100467815

;

InterPros

IPR011009	; Kinase-like_dom.
IPR000719	; Prot_kinase_cat_dom.
IPR017441	; Protein_kinase_ATP_BS.
IPR002290	; Ser/Thr_dual-sp_kinase_dom.
IPR008271	; Ser/Thr_kinase_AS.

Pfam

PF00069

; Pkinase; 1.

SMARTs

SM00220

; S_TKc; 1.

Prosites

PS00107	; PROTEIN_KINASE_ATP; 1.
PS50011	; PROTEIN_KINASE_DOM; 1.
PS00108	; PROTEIN_KINASE_ST; 1.

Prints

Created Date

20-Feb-2013