EKS-AIM-00397

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EKS-AIM-00397

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
Other/WEE	371.0	3.0E-111	214	488	275

Status

Reviewed

Ensembl Protein

ENSAMEP00000015995

UniProt Accession

D2HHP1;

Protein Name

Wee1-like protein kinase 2

Protein Synonyms/Alias

Wee1-like protein kinase 1B; Wee1B kinase;

Gene Name

WEE2

Gene Synonyms/Alias

WEE2; WEE1B; PANDA_010640;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
ENSAMEG00000015162	ENSAMEP00000015995	ENSAMET00000016662

Organism

Ailuropoda melanoleuca

Functional Description

Oocyte-specific protein tyrosine kinase thatphosphorylates and inhibits CDK1 and acts as a key regulator of meiosis during both prophase I and metaphase II. Required to maintain meiotic arrest in oocytes during the germinal vesicle (GV) stage, a long period of quiescence at dictyate prophase I, by phosphorylating CDK1 at 'Tyr-15', leading to inhibit CDK1 activity and prevent meiotic reentry. Also required for metaphase II exit during egg activation by phosphorylating CDK1 at 'Tyr-15', to ensure exit from meiosis in oocytes and promote pronuclear formation (By similarity).

Protein Length

565

Protein Sequence
(FASTA)

MDDSSINKEL KQKLNFSYCE EESESEGQEA WETRDAHSQI PDRAEGQESE AKFTPPGPPL 60

SSVHEVGTFQ EKTKKSPEQV LMTPVSGFRN YPETPAQPDS RSKLLDCESP FTPKGLLSQS 120

VISSTEKIPS RGSKHLRFTP VPFVDEMTSS ALVNINPFTP ESYRKQFLKS NGKRKTRGDF 180

EEAGPGEGNV EQGLPAKRCV LQETNMASRY EKEFLEVEKI GVGEFGTVYK CIKRLDGCVY 240

AIKRSTKPFA GLSNENLALH EVYAHAVLGH HPHVVRYYSA WAEDDHMIIQ NEYCNGGSLQ 300

TAISENTKSG NHFQEPKLKD ILLQISLGLK YIHSSGMVHL DIKPSNIFIC HKMQSDSPVV 360

PEEIENEADW FLSANVMYKI GDLGHVTSIS KPKVEEGDSR FLANEILQED YQHLPKADIF 420

ALGLTIAVAA GAESLPTNGA AWHHIREGKL PDIPQKLSEE FYNLLKNMIH PDPRERPSAA 480

ALARSRVLRP SLGKAEELQQ QLNLEKFKTA TLERELREAQ QAWFSQEERG DAGVSGTPTG 540

SRSTKRLVGG KSAKSSSFTW GKSSP 565

Nucleotide Sequence
(FASTA)

ATGGATGACA GCAGTATTAA CAAAGAACTA AAGCAGAAGT TAAACTTTTC CTACTGTGAA 60

GAAGAGAGTG AGAGTGAAGG GCAGGAGGCA TGGGAGACCA GGGATGCCCA CAGCCAAATC 120

CCAGACAGGG CTGAAGGTCA GGAGTCAGAG GCAAAGTTCA CACCTCCCGG GCCTCCCCTT 180

AGTAGCGTCC ATGAAGTCGG CACATTTCAG GAAAAAACCA AGAAGAGTCC AGAACAGGTT 240

TTGATGACTC CAGTGTCAGG TTTTCGCAAC TATCCAGAGA CACCAGCCCA GCCAGACAGC 300

AGGAGCAAGT TGCTGGATTG TGAGAGTCCC TTCACTCCCA AGGGTCTGCT AAGCCAGTCG 360

GTGATTTCTT CAACAGAGAA GATCCCTTCC AGAGGCTCTA AACATTTGAG GTTCACGCCT 420

GTTCCCTTCG TGGATGAGAT GACCTCATCG GCTCTGGTCA ATATTAATCC CTTTACCCCA 480

GAGTCCTATA GAAAACAATT CCTCAAATCC AATGGCAAGA GGAAAACCCG AGGAGATTTT 540

GAGGAAGCTG GTCCAGGGGA AGGCAATGTG GAACAAGGGC TGCCTGCCAA GAGATGTGTT 600

CTACAAGAAA CCAACATGGC TTCCCGCTAT GAAAAAGAAT TCTTGGAGGT AGAAAAAATT 660

GGAGTTGGGG AATTTGGGAC AGTCTACAAG TGTATTAAGA GGCTGGATGG ATGTGTTTAT 720

GCAATAAAAC GTTCCACAAA ACCTTTTGCA GGGTTATCAA ATGAGAATTT GGCTTTGCAT 780

GAGGTTTATG CTCACGCAGT GCTTGGGCAT CACCCCCATG TGGTACGTTA CTACTCTGCG 840

TGGGCAGAAG ATGACCACAT GATCATTCAG AATGAATACT GCAATGGTGG GAGCTTGCAG 900

ACTGCTATAT CTGAAAACAC AAAGTCTGGC AATCATTTCC AGGAGCCGAA ACTCAAGGAC 960

ATCCTTCTGC AGATTTCCCT TGGGCTTAAG TACATCCACA GCTCCGGCAT GGTGCACCTG 1020

GACATCAAAC CTAGTAACAT CTTCATTTGT CATAAGATGC AAAGTGACTC TCCTGTAGTC 1080

CCAGAAGAGA TTGAAAATGA AGCTGATTGG TTTCTCTCTG CCAACGTGAT GTATAAAATT 1140

GGTGACTTGG GTCATGTGAC ATCAATAAGC AAACCAAAAG TGGAGGAGGG AGATAGTCGC 1200

TTCCTGGCTA ATGAGATTTT ACAAGAGGAT TATCAGCACC TTCCCAAAGC AGACATATTT 1260

GCCTTGGGAT TAACAATTGC AGTGGCTGCA GGAGCAGAGT CATTACCCAC CAATGGTGCC 1320

GCATGGCATC ATATTCGTGA GGGGAAACTT CCAGACATTC CTCAGAAGCT CTCAGAAGAA 1380

TTTTACAATC TACTCAAGAA CATGATCCAC CCTGACCCAA GGGAGAGACC TTCTGCAGCA 1440

GCTCTGGCCA GAAGTCGAGT TCTCCGGCCT TCCCTTGGGA AAGCAGAAGA GCTCCAGCAG 1500

CAGCTTAACT TGGAAAAGTT CAAGACAGCC ACACTGGAAA GGGAACTGAG AGAAGCTCAG 1560

CAGGCTTGGT TCTCCCAGGA AGAGCGTGGT GATGCTGGGG TCTCTGGGAC CCCCACAGGA 1620

TCAAGAAGCA CCAAACGCCT GGTGGGAGGA AAAAGTGCAA AGTCTTCAAG CTTCACCTGG 1680

GGGAAGTCTT CCCCATAA 1698

Domain Profile

S: 1     FeevekiGkGefgevfkvvkredgklyAikkskkplkglskekkalrEvtahavlgqhen  60

         F evekiG+Gefg+v+k++kr+dg++yAik+s kp++gls+e+ al+Ev+ahavlg+h++

Q: 214   FLEVEKIGVGEFGTVYKCIKRLDGCVYAIKRSTKPFAGLSNENLALHEVYAHAVLGHHPH  273

         889*********************************************************

S: 61    vvryisaWeeddslyiqtElCeggsLeaalqeceakkkaleeaelkkilldlllglrylH  120

         vvry+saW+edd+++iq+E+C+ggsL+ a++e++++ ++++e +lk+ill+++lgl+y+H

Q: 274   VVRYYSAWAEDDHMIIQNEYCNGGSLQTAISENTKSGNHFQEPKLKDILLQISLGLKYIH  333

         ************************************************************

S: 121   ssslvHlDikpenifi.......................tkevtlklgDlGlvtelnsed  157

         ss +vHlDikp+nifi                       +++v++k+gDlG+vt++   +

Q: 334   SSGMVHLDIKPSNIFIchkmqsdspvvpeeieneadwflSANVMYKIGDLGHVTSI---S  390

         ********************************************************...*

S: 158   kskveEGDsrylakEilqeeyskl.kaDiFslgltilevaanvelPsnGeaWhklRkgkl  216

         k+kveEGDsr+la+Eilqe+y++l kaDiF+lglti+++a++++lP+nG aWh++R+gkl

Q: 391   KPKVEEGDSRFLANEILQEDYQHLpKADIFALGLTIAVAAGAESLPTNGAAWHHIREGKL  450

         ************************************************************

S: 217   pdeilkelsedlrellklmlepdperRptaeellkhevl  255

         p +i ++lse++ +llk+m++pdp++Rp+a++l++++vl

Q: 451   P-DIPQKLSEEFYNLLKNMIHPDPRERPSAAALARSRVL  488

         *.*******************************998875

Domain Sequence
(FASTA)

FLEVEKIGVG EFGTVYKCIK RLDGCVYAIK RSTKPFAGLS NENLALHEVY AHAVLGHHPH 60

VVRYYSAWAE DDHMIIQNEY CNGGSLQTAI SENTKSGNHF QEPKLKDILL QISLGLKYIH 120

SSGMVHLDIK PSNIFICHKM QSDSPVVPEE IENEADWFLS ANVMYKIGDL GHVTSISKPK 180

VEEGDSRFLA NEILQEDYQH LPKADIFALG LTIAVAAGAE SLPTNGAAWH HIREGKLPDI 240

PQKLSEEFYN LLKNMIHPDP RERPSAAALA RSRVL 275

Keyword

ATP-binding; Coiled coil; Complete proteome; Kinase; Magnesium; Meiosis; Metal-binding; Nucleotide-binding; Nucleus; Phosphoprotein; Reference proteome; Transferase; Tyrosine-protein kinase.

Sequence Source

Ensembl

Orthology

Ortholog group

Anolis carolinensis"; ?>Bos taurus"; ?>Callithrix jacchus"; ?>Canis familiaris"; ?>Cavia porcellus"; ?>Danio rerio"; ?>Equus caballus"; ?>Gallus gallus"; ?>Gasterosteus aculeatus"; ?>Gorilla gorilla"; ?>Homo sapiens"; ?>Ictidomys tridecemlineatus"; ?>Latimeria chalumnae"; ?>Loxodonta africana"; ?>Meleagris gallopavo"; ?>Monodelphis domestica"; ?>Mus musculus"; ?>Mustela putorius furo"; ?>Myotis lucifugus"; ?>Ochotona princeps"; ?>Oreochromis niloticus"; ?>Ornithorhynchus anatinus"; ?>Oryctolagus cuniculus"; ?>Oryzias latipes"; ?>Otolemur garnettii"; ?>Pan troglodytes"; ?>Pongo abelii"; ?>Pteropus vampyrus"; ?>Rattus norvegicus"; ?>Sarcophilus harrisii"; ?>Sus scrofa"; ?>Taeniopygia guttata"; ?>Takifugu rubripes"; ?>Tetraodon nigroviridis"; ?>Xenopus tropicalis"; ?>

Gene Ontology

GO:0005813	; C:centrosome
GO:0005737	; C:cytoplasm
GO:0005634	; C:nucleus
GO:0005524	; F:ATP binding
GO:0000287	; F:magnesium ion binding
GO:0004715	; F:non-membrane spanning protein tyrosine kinase activity
GO:0004674	; F:protein serine/threonine kinase activity
GO:0007343	; P:egg activation
GO:0007143	; P:female meiosis
GO:0035038	; P:female pronucleus assembly
GO:0007137	; P:meiotic metaphase II
GO:0007128	; P:meiotic prophase I
GO:0007067	; P:mitosis
GO:0060283	; P:negative regulation of oocyte development
GO:0060631	; P:regulation of meiosis I

KEGG

aml:100468598

;

InterPros

IPR011009	; Kinase-like_dom.
IPR000719	; Prot_kinase_cat_dom.
IPR017441	; Protein_kinase_ATP_BS.
IPR008271	; Ser/Thr_kinase_AS.
IPR017164	; Wee1-like_protein_kinase.

Pfam

PF00069

; Pkinase; 1.

SMARTs

Prosites

PS00107	; PROTEIN_KINASE_ATP; 1.
PS50011	; PROTEIN_KINASE_DOM; 1.
PS00108	; PROTEIN_KINASE_ST; 1.

Prints

Created Date

20-Feb-2013