EKS-BOT-00064

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EKS-BOT-00064

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
CMGC/CDK	373.7	5.7E-112	706	998	293

Status

Reviewed

Ensembl Protein

ENSBTAP00000002003

UniProt Accession

E1BB52;

Protein Name

Cyclin-dependent kinase 13

Protein Synonyms/Alias

CDC2-related protein kinase 5; Cell division cycle 2-like protein kinase 5; Cell division protein kinase 13;

Gene Name

CDK13

Gene Synonyms/Alias

CDK13; CDC2L, CDC2L5;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
ENSBTAG00000001528	ENSBTAP00000002003	ENSBTAT00000002003

Organism

Bos taurus

Functional Description

Cyclin-dependent kinase which displays CTD kinaseactivity and is required for RNA splicing. Has CTD kinase activity by hyperphosphorylating the C-terminal heptapeptide repeat domain (CTD) of the largest RNA polymerase II subunit RPB1, thereby acting as a key regulator of transcription elongation. Required for RNA splicing, probably by phosphorylating SRSF1/SF2. Required during hematopoiesis (By similarity).

Protein Length

1512

Protein Sequence
(FASTA)

MPSSSDTALG GGGGLSWAEK KLEERRKRRR FLSPQQPPLL LPLLQPQLLQ PPPPPPPLLF 60

LAAPGTAAAA AAAAAASSSC FSPGPPLEVK RLARGKRRAG GRQKRRRGPR AGQEAEKRRV 120

FSLPQPQQDG GGGASSGGGV TPLVEYEDVS SQSEQGLLLG GASAATAATA AGGTGGSGGS 180

PASSSGTQRR GEGSERRPRR DRRSSSGRSK DRHREHRRRD GQRGGGEASK SRSRHGHGGE 240

ERAEAGKSGS SSSSGGRRKS ASATSSSSSS RKDRDPKAHR SRTKSSKEPP SAYKEPPKAY 300

REDKTEPKAY RRRQRSLSPL GGRDDSPVSH RASQSLRNRK SPSPAGGGSS PYSRRLARSP 360

SPYSRRRSPS YSRHSSYERG GDVSPSPYSS SSWRRSRSPY SPVIRRSAKS RSRSPYSSRH 420

SRSRSRHRLS RSRSRHSSIS PSTLTLKSSL AAELNKNKKA RAAEAARAAE AAKAAEAAKA 480

AEAAAKAAKA ASTSTPTKGN TETGASASQT NHVKDVKKLK TEHAPSPSSG GTLKNDKAKT 540

KPPLQVTKVD NNLIVDKATK KAVVVGKESK SAATKEEPVS LKEKTKPLTP SIGAKEKEQH 600

VALVTSTLPP LPLPPMLPED KDADSLRGNI SVKAVKKEVE KKLRCLLADL PLPPELPGGD 660

DLSKSPEEKK TATQLHNKRR PKICGPRFGE IKEKDIDWGK RCVDKFDIIG IIGEGTYGQV 720

YKARDKDTGE MVALKKVRLD NEKEGFPITA IREIKILRQL THQSIINMKE IVTDKEDALD 780

FKKDKGAFYL VFEYMDHDLM GLLESGLVHF NENHIKSFMR QLMEGLDYCH KKNFLHRDIK 840

CSNILLNNRG QIKLADFGLA RLYSSEESRP YTNKVITLWY RPPELLLGEE RYTPAIDVWS 900

CGCILGELFT KKPIFQANQE LAQLELISRI CGSPCPAVWP DVIKLPYFNT MKPKKQYRRK 960

LREEFVFIPA AALDLFDYML ALDPSKRCTA EQALQCEFLR DVEPSKMPPP DLPLWQDCHE 1020

LWSKKRRRQK QMGMTDDVST VKAPRKDLSL GMDDSRTSTP QSVLPSSQLK PQGNSNAAPV 1080

KTGPGQQLNH SELAILLNLL QSKTSVNMAD FVQVLNIKVN SETQQQLNKI NLPAGILATG 1140

EKQTDPSTPQ QESSKPLGGI QPSQNMQPKV EPDAAQAAVQ SAFAVLLTQL IKAQQSKQKD 1200

VLLEERENGS GHEAPLQLRP PPEPATPASG QDDLIQHQDM RLLELTPEPD RPRILPPDQR 1260

PPEPPEPPPV TEEDLDYRTE NQHVPTTSSS LTDPHAGVKA ALLQLLAQHQ PQDDPKRESG 1320

IDYQAGDTYV PSSDYKDNFG SSSFSSAPYV SNDGLGSASA PPLERRSFMG NSDIQSLDNY 1380

STTSSHSGGP PQPSAFSESF PSSVAGYGDI YLNTGPMLFS GDKDHRFEYS HGPIAVLANS 1440

SDPSTGPEST HPLPAKMHNY NYGGSLQETP GGHGLMHGQT WTSPAQGPGY SQGYRGHIST 1500

SAGRGRGRGL PY 1512

Nucleotide Sequence
(FASTA)

ATGCCGAGCA GCTCGGACAC GGCGCTGGGG GGAGGCGGGG GCTTGAGCTG GGCGGAGAAG 60

AAGTTGGAGG AACGCCGCAA ACGGAGGCGA TTCCTGTCCC CTCAGCAGCC GCCGCTGCTG 120

TTGCCGCTCC TGCAGCCGCA GCTCCTGCAA CCGCCGCCGC CCCCGCCGCC TCTGCTCTTC 180

CTGGCTGCTC CCGGCACGGC CGCCGCCGCA GCCGCCGCCG CCGCGGCCTC CTCCTCTTGC 240

TTCAGCCCGG GTCCCCCTCT GGAGGTCAAG CGGCTGGCGA GAGGCAAGAG GCGCGCAGGA 300

GGGCGGCAGA AGCGGCGCCG CGGGCCCCGC GCCGGGCAGG AGGCGGAGAA GCGTCGGGTC 360

TTCTCGCTGC CCCAGCCGCA GCAGGACGGC GGTGGCGGTG CTAGTAGCGG CGGGGGTGTG 420

ACCCCGCTGG TGGAGTACGA GGATGTAAGC TCCCAGTCCG AGCAGGGGCT GCTGCTGGGG 480

GGGGCCAGCG CGGCAACGGC GGCGACGGCT GCCGGGGGAA CGGGGGGCAG CGGCGGGAGT 540

CCGGCCTCCT CCTCCGGCAC CCAGCGTCGC GGGGAAGGGT CGGAGCGCAG GCCCCGCCGG 600

GACCGCCGCA GCAGCAGCGG CCGCAGCAAG GATCGCCACC GCGAGCACCG GCGGCGGGAC 660

GGGCAGCGCG GCGGCGGCGA GGCCTCCAAG TCCCGCAGCC GCCACGGCCA CGGCGGCGAG 720

GAGCGGGCCG AGGCCGGCAA GAGCGGTAGC AGCAGCAGCA GCGGCGGCCG GCGGAAAAGC 780

GCCTCGGCCA CGTCCAGCAG CAGCAGCAGC CGCAAGGACC GAGACCCGAA GGCCCATCGA 840

AGCCGGACTA AATCGTCCAA AGAACCGCCT TCGGCCTATA AGGAGCCGCC CAAGGCCTAC 900

CGGGAGGACA AGACCGAGCC CAAGGCCTAC AGGCGGCGGC AGCGGTCCCT GAGTCCGCTG 960

GGAGGCCGGG ACGACAGCCC GGTGTCTCAC CGGGCCTCGC AGAGCCTGCG GAACCGCAAG 1020

TCTCCCAGTC CGGCGGGAGG TGGCAGCAGC CCCTACTCGC GGCGGCTGGC CCGCTCCCCG 1080

AGCCCCTACA GCCGCCGCCG CTCCCCCAGC TACAGCCGCC ACAGCTCTTA CGAGCGCGGC 1140

GGCGACGTGT CCCCTAGCCC CTACAGCAGC AGCAGCTGGC GCCGCTCCCG GAGCCCCTAC 1200

AGCCCCGTGA TCAGACGGTC TGCCAAATCA CGAAGCAGAA GCCCATATTC ATCTAGGCAT 1260

TCAAGATCCC GTAGCAGGCA TAGATTGTCT AGATCCAGAA GTCGTCATTC TAGCATTTCT 1320

CCTAGCACAC TAACTCTGAA GAGTAGCCTG GCAGCTGAAT TGAACAAGAA TAAGAAAGCA 1380

CGAGCTGCAG AGGCAGCACG CGCTGCAGAG GCCGCTAAGG CCGCAGAAGC AGCCAAGGCT 1440

GCTGAGGCTG CTGCCAAAGC TGCCAAAGCT GCCAGCACTT CTACACCTAC CAAGGGGAAC 1500

ACAGAAACTG GTGCCAGTGC ATCACAAACC AACCATGTGA AGGATGTGAA GAAACTTAAA 1560

ACTGAGCATG CACCTTCTCC CTCAAGCGGT GGAACTTTAA AAAATGACAA GGCAAAAACA 1620

AAGCCACCTC TTCAGGTAAC AAAAGTGGAT AATAATTTGA TTGTAGATAA AGCCACCAAG 1680

AAAGCAGTTG TAGTTGGAAA GGAGAGTAAA TCTGCTGCTA CAAAGGAGGA ACCAGTATCT 1740

CTTAAAGAGA AAACGAAACC ACTTACACCA AGCATAGGAG CCAAGGAGAA GGAGCAACAC 1800

GTGGCTTTAG TGACCTCTAC CTTACCACCC TTACCTTTGC CTCCCATGCT GCCTGAAGAT 1860

AAAGATGCCG ATAGCTTGCG AGGAAATATT TCAGTAAAAG CGGTTAAAAA AGAAGTAGAA 1920

AAGAAACTGC GATGTCTACT TGCTGATTTA CCACTGCCCC CTGAGCTACC AGGAGGAGAT 1980

GATCTCTCAA AGAGTCCAGA GGAAAAGAAA ACAGCAACAC AGTTACATAA TAAAAGGAGG 2040

CCTAAAATAT GTGGGCCTCG CTTTGGTGAA ATCAAAGAGA AAGATATTGA CTGGGGAAAA 2100

CGCTGCGTGG ATAAATTTGA TATCATCGGA ATTATTGGAG AAGGCACCTA TGGACAAGTT 2160

TACAAGGCCA GGGATAAAGA CACTGGAGAA ATGGTAGCCT TAAAAAAAGT GCGCCTGGAT 2220

AATGAAAAGG AAGGGTTTCC TATTACAGCA ATTCGAGAAA TTAAAATTCT CCGGCAGCTT 2280

ACCCACCAGA GTATTATCAA TATGAAGGAA ATAGTGACCG ATAAAGAAGA TGCTTTGGAC 2340

TTTAAGAAGG ATAAAGGTGC ATTTTATTTG GTGTTTGAAT ATATGGACCA TGATCTGATG 2400

GGACTGTTGG AATCAGGCTT GGTTCATTTT AATGAAAATC ACATAAAGTC ATTTATGAGA 2460

CAGCTCATGG AAGGTCTGGA TTATTGTCAT AAGAAGAACT TTTTGCATCG AGATATTAAA 2520

TGTTCAAATA TTCTTCTAAA CAATAGAGGA CAGATAAAGC TTGCAGATTT TGGACTTGCT 2580

CGATTGTATA GCTCAGAAGA AAGTCGGCCA TATACAAACA AAGTCATCAC TTTGTGGTAC 2640

CGTCCACCTG AACTGCTGCT GGGAGAAGAA AGGTATACAC CAGCTATCGA TGTGTGGAGC 2700

TGTGGATGTA TCCTTGGTGA ACTCTTCACT AAAAAACCTA TATTTCAAGC AAATCAGGAG 2760

CTTGCACAAC TAGAATTAAT AAGCCGTATA TGTGGGAGTC CATGTCCTGC AGTGTGGCCT 2820

GATGTAATCA AACTACCGTA TTTCAACACC ATGAAACCAA AGAAGCAATA TCGTCGAAAG 2880

TTAAGAGAAG AATTTGTTTT TATTCCTGCA GCCGCACTAG ACTTATTTGA TTACATGCTC 2940

GCCTTGGATC CTAGTAAGCG CTGCACTGCA GAGCAGGCTC TTCAGTGTGA GTTCCTACGG 3000

GATGTGGAAC CCTCAAAAAT GCCTCCACCA GACCTTCCAT TATGGCAAGA CTGTCATGAG 3060

TTATGGAGTA AAAAGCGAAG ACGACAGAAG CAGATGGGCA TGACTGATGA TGTTTCCACA 3120

GTTAAAGCCC CCAGAAAGGA CTTGTCTCTG GGCATGGATG ACAGCAGAAC TAGCACGCCC 3180

CAGAGTGTGC TGCCGTCTTC ACAGCTGAAA CCTCAGGGCA ACTCGAATGC AGCACCTGTA 3240

AAAACAGGCC CTGGACAGCA GTTAAACCAC AGTGAATTGG CAATTCTGCT AAACCTACTA 3300

CAGTCTAAAA CAAGTGTTAA TATGGCTGAT TTTGTCCAAG TGTTGAACAT TAAGGTAAAC 3360

TCTGAGACTC AGCAGCAGCT AAATAAAATA AACCTTCCTG CTGGAATTTT GGCAACAGGT 3420

GAAAAACAGA CAGATCCATC AACACCACAA CAGGAGTCTT CGAAACCATT GGGAGGAATT 3480

CAGCCTTCTC AGAACATGCA GCCTAAAGTG GAGCCTGATG CGGCCCAGGC GGCTGTGCAG 3540

AGCGCATTTG CGGTTCTGTT GACTCAGTTA ATAAAGGCTC AGCAGTCAAA GCAGAAAGAC 3600

GTGCTGCTGG AAGAGAGGGA AAATGGGTCG GGTCATGAAG CACCATTACA GCTCCGGCCG 3660

CCTCCAGAGC CTGCCACGCC GGCGTCGGGG CAAGATGACC TGATTCAGCA CCAAGATATG 3720

AGGCTATTGG AGCTCACACC AGAACCGGAC CGGCCTCGTA TTCTGCCTCC CGATCAGCGA 3780

CCTCCTGAAC CTCCGGAGCC ACCACCAGTC ACTGAAGAAG ATCTAGATTA CCGGACAGAA 3840

AACCAGCATG TACCCACCAC TAGTTCTTCG TTAACTGACC CTCATGCCGG AGTGAAAGCA 3900

GCCCTGTTAC AGCTCCTTGC TCAGCATCAG CCCCAGGACG ACCCCAAAAG AGAAAGTGGT 3960

ATTGATTACC AAGCAGGAGA TACTTATGTA CCTTCTTCAG ACTACAAGGA CAATTTTGGA 4020

TCCTCTTCTT TCTCTTCTGC TCCTTATGTT AGCAATGACG GTCTTGGAAG CGCCTCTGCT 4080

CCACCATTGG AACGACGTAG CTTCATGGGA AACTCAGATA TTCAGTCCTT GGATAACTAC 4140

AGTACTACTT CATCTCATTC TGGTGGTCCA CCTCAGCCTT CTGCCTTTTC CGAGTCATTT 4200

CCCAGTTCGG TAGCTGGATA CGGAGACATT TACCTCAATA CAGGTCCCAT GTTGTTTAGT 4260

GGAGACAAGG ACCATAGATT TGAATATAGC CATGGCCCCA TTGCAGTGCT GGCAAACAGC 4320

AGTGACCCTT CCACAGGGCC AGAGAGTACT CATCCTTTGC CAGCAAAGAT GCACAACTAT 4380

AACTATGGTG GTTCCTTACA GGAAACCCCA GGTGGCCATG GCCTCATGCA TGGACAGACC 4440

TGGACTTCTC CTGCCCAAGG ACCTGGGTAT TCACAAGGAT ACAGGGGACA CATTAGCACA 4500

TCAGCTGGCA GAGGTCGAGG CAGAGGGTTA CCATACTGA 4539

Domain Profile

S: 1     yeklekigeGtygkvykakdketgevvAlKkirlekekegvpitalrEisllkelkhkni  60

         ++ +  igeGtyg+vyka+dk+tge+vAlKk+rl++ekeg+pita+rEi++l++l+h+ i

Q: 706   FDIIGIIGEGTYGQVYKARDKDTGEMVALKKVRLDNEKEGFPITAIREIKILRQLTHQSI  765

         667889******************************************************

S: 61    vkLlevva.........kdkkklyLvfefleqdLkkllkkkkkkklsleevkslllqlle  111

         ++++e+v+         kdk  +yLvfe++++dL+ ll++    +++++++ks+++ql+e

Q: 766   INMKEIVTdkedaldfkKDKGAFYLVFEYMDHDLMGLLESGLV-HFNENHIKSFMRQLME  824

         ****************8899********************999.****************

S: 112   glaylHsnkilHRDlKpqNlLiskegklklaDfGlarafssplk.tytkevvTlwYraPe  170

         gl+y+H++++lHRD+K++N+L++++g++klaDfGlar +ss+++  yt++v+TlwYr+Pe

Q: 825   GLDYCHKKNFLHRDIKCSNILLNNRGQIKLADFGLARLYSSEESrPYTNKVITLWYRPPE  884

         ***************************************7665527**************

S: 171   lLlgakeYstavDiWsvgcilaelltrkplfqgkseidqlerifkllgtpsekvwpevsk  230

         lLlg+++Y+ a+D+Ws+gcil el+t+kp+fq+++e  qle i++++g+p  +vwp+v k

Q: 885   LLLGEERYTPAIDVWSCGCILGELFTKKPIFQANQELAQLELISRICGSPCPAVWPDVIK  944

         ************************************************************

S: 231   lpeykksfpkkkkk.klkekvkklkekaldllekllaldpkkRisakealqhky  283

         lp +++  pkk+++ kl+e++  +  +aldl++ +laldp+kR++a++alq ++

Q: 945   LPYFNTMKPKKQYRrKLREEFVFIPAAALDLFDYMLALDPSKRCTAEQALQCEF  998

         *****7777777777******999999************************988

Domain Sequence
(FASTA)

FDIIGIIGEG TYGQVYKARD KDTGEMVALK KVRLDNEKEG FPITAIREIK ILRQLTHQSI 60

INMKEIVTDK EDALDFKKDK GAFYLVFEYM DHDLMGLLES GLVHFNENHI KSFMRQLMEG 120

LDYCHKKNFL HRDIKCSNIL LNNRGQIKLA DFGLARLYSS EESRPYTNKV ITLWYRPPEL 180

LLGEERYTPA IDVWSCGCIL GELFTKKPIF QANQELAQLE LISRICGSPC PAVWPDVIKL 240

PYFNTMKPKK QYRRKLREEF VFIPAAALDL FDYMLALDPS KRCTAEQALQ CEF 293

Keyword

Acetylation; ATP-binding; Complete proteome; Kinase; mRNA processing; mRNA splicing; Nucleotide-binding; Nucleus; Phosphoprotein; Reference proteome; Serine/threonine-protein kinase; Transferase.

Sequence Source

Ensembl

Orthology

Ortholog group

Ailuropoda melanoleuca"; ?>Anolis carolinensis"; ?>Callithrix jacchus"; ?>Canis familiaris"; ?>Cavia porcellus"; ?>Ciona intestinalis"; ?>Danio rerio"; ?>Drosophila melanogaster"; ?>Equus caballus"; ?>Felis catus"; ?>Gallus gallus"; ?>Gasterosteus aculeatus"; ?>Gorilla gorilla"; ?>Homo sapiens"; ?>Ictidomys tridecemlineatus"; ?>Latimeria chalumnae"; ?>Loxodonta africana"; ?>Macaca mulatta"; ?>Meleagris gallopavo"; ?>Monodelphis domestica"; ?>Mus musculus"; ?>Mustela putorius furo"; ?>Myotis lucifugus"; ?>Nomascus leucogenys"; ?>Oreochromis niloticus"; ?>Oryctolagus cuniculus"; ?>Otolemur garnettii"; ?>Pan troglodytes"; ?>Pelodiscus sinensis"; ?>Petromyzon marinus"; ?>Pongo abelii"; ?>Sarcophilus harrisii"; ?>Sus scrofa"; ?>Taeniopygia guttata"; ?>Takifugu rubripes"; ?>Tetraodon nigroviridis"; ?>Xenopus tropicalis"; ?>Xiphophorus maculatus"; ?>Schizosaccharomyces pombe"; ?>

Gene Ontology

GO:0019908	; C:nuclear cyclin-dependent protein kinase holoenzyme complex
GO:0016607	; C:nuclear speck
GO:0005524	; F:ATP binding
GO:0004693	; F:cyclin-dependent protein kinase activity
GO:0008353	; F:RNA polymerase II carboxy-terminal domain kinase activity
GO:0000380	; P:alternative mRNA splicing, via spliceosome
GO:0007049	; P:cell cycle
GO:0030097	; P:hemopoiesis
GO:0070816	; P:phosphorylation of RNA polymerase II C-terminal domain

KEGG

bta:511147

;

InterPros

IPR011009	; Kinase-like_dom.
IPR000719	; Prot_kinase_cat_dom.
IPR017441	; Protein_kinase_ATP_BS.
IPR002290	; Ser/Thr_dual-sp_kinase_dom.
IPR008271	; Ser/Thr_kinase_AS.

Pfam

PF00069

; Pkinase; 1.

SMARTs

SM00220

; S_TKc; 1.

Prosites

PS00107	; PROTEIN_KINASE_ATP; 1.
PS50011	; PROTEIN_KINASE_DOM; 1.
PS00108	; PROTEIN_KINASE_ST; 1.

Prints

Created Date

20-Feb-2013