| Tag | Content |
|---|
| EKPD ID | EKS-BOT-00226 |
| Classification | | Group/Family | Score | E-Value | Start | End | Domain Length |
|---|
| STE/STE7 | 386.8 | 5.4E-116 | 53 | 314 | 262 |
|
| Status | Reviewed |
| Ensembl Protein | ENSBTAP00000002115 |
| UniProt Accession | Q5E9X2; |
| Protein Name | Dual specificity mitogen-activated protein kinase kinase 6 |
| Protein Synonyms/Alias | MAP kinase kinase 6; MAPKK 6; MAPK/ERK kinase 6; MEK 6; |
| Gene Name | MAP2K6 |
| Gene Synonyms/Alias | MAP2K6; |
| Ensembl Information | |
| Organism | Bos taurus |
| Functional Description | Dual specificity protein kinase which acts as anessential component of the MAP kinase signal transduction pathway. With MAP3K3/MKK3, catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in the MAP kinases p38 MAPK11, MAPK12, MAPK13 and MAPK14 and plays an important role in the regulation of cellular responses to cytokines and all kinds of stresses. Especially, MAP2K3/MKK3 and MAP2K6/MKK6 are both essential for the activation of MAPK11 and MAPK13 induced by environmental stress, whereas MAP2K6/MKK6 is the major MAPK11 activator in response to TNF. MAP2K6/MKK6 also phosphorylates and activates PAK6. The p38 MAP kinase signal transduction pathway leads to direct activation of transcription factors. Nuclear targets of p38 MAP kinase include the transcription factors ATF2 and ELK1. Within the p38 MAPK signal transduction pathway, MAP3K6/MKK6 mediates phosphorylation of STAT4 through MAPK14 activation, and is therefore required for STAT4 activation and STAT4-regulated gene expression in response to IL-12 stimulation. The pathway is also crucial for IL-6-induced SOCS3 expression and down-regulation of IL-6-mediated gene induction; and for IFNG- dependent gene transcription. Has a role in osteoclast differentiation through NF-kappa-B transactivation by TNFSF11, and in endochondral ossification and since SOX9 is another likely downstream target of the p38 MAPK pathway. MAP2K6/MKK6 mediates apoptotic cell death in thymocytes. Acts also as a regulator for melanocytes dendricity, through the modulation of Rho family GTPases (By similarity). |
| Protein Length | 334 |
Protein Sequence
(FASTA) | | MSQSKGKKRN PGLKIPKEAF EQPQTSSTPP RDLDSKACIS IGNQNFEVKA DDLEPIVELG 60 | | RGAYGVVEKM RHVPSEQIMA VKRIRATVNS QEQKRLLMDL DISMRTVDCP FTVTFYGALF 120 | | REGDVWICME LMDTSLDKFY KQVIDKGQTI PEDILGKIAV SIVKALEHLH SKLSVIHRDV 180 | | KPSNVLINAL GQVKMCDFGI SGYLVDSVAK TIDAGCKPYM APERINPELN QKGYSVKSDI 240 | | WSLGITMIEL AILRFPYDSW GTPFQQLKQV VEEPSPQLPA DKFSEEFVDF TSQCLKKNSK 300 | | ERPTYPELMQ HPFFTLHESK ATDVASFVKS ILGD 334 |
|
Nucleotide Sequence
(FASTA) | | ATGTCTCAGT CGAAAGGCAA GAAGCGAAAC CCTGGCCTTA AAATTCCCAA AGAAGCATTT 60 | | GAACAACCTC AGACCAGTTC CACGCCGCCG CGGGATTTAG ACTCCAAAGC TTGCATTTCT 120 | | ATTGGAAATC AGAACTTTGA GGTGAAGGCG GATGACCTGG AGCCCATCGT GGAGCTGGGC 180 | | CGGGGGGCGT ACGGGGTGGT GGAGAAGATG CGGCACGTGC CCAGCGAGCA GATCATGGCG 240 | | GTGAAGCGGA TCCGGGCCAC GGTAAACAGC CAAGAGCAGA AGCGGCTCCT GATGGATCTG 300 | | GATATCTCCA TGAGGACCGT GGACTGTCCC TTCACGGTCA CCTTTTACGG CGCACTCTTC 360 | | CGTGAGGGGG ATGTCTGGAT CTGCATGGAG CTCATGGACA CGTCCCTAGA TAAATTCTAC 420 | | AAACAAGTGA TCGATAAAGG CCAAACAATT CCAGAGGACA TCTTAGGGAA AATAGCAGTT 480 | | TCTATTGTAA AAGCCTTAGA GCATTTGCAC AGTAAGCTGT CTGTCATTCA CCGAGATGTC 540 | | AAGCCCTCCA ACGTGCTCAT CAACGCTCTG GGCCAGGTGA AGATGTGCGA CTTCGGAATC 600 | | AGCGGCTACC TGGTTGACTC CGTGGCTAAA ACCATCGATG CCGGATGCAA ACCGTACATG 660 | | GCCCCCGAGA GAATAAACCC AGAGCTCAAC CAGAAGGGAT ACAGCGTGAA GTCCGACATC 720 | | TGGAGTTTGG GCATCACCAT GATCGAGCTG GCCATCCTCC GGTTTCCCTA CGACTCGTGG 780 | | GGGACTCCTT TCCAGCAGCT CAAACAGGTG GTGGAGGAGC CGTCGCCGCA GCTGCCCGCA 840 | | GACAAGTTCT CCGAAGAGTT TGTTGACTTT ACCTCACAGT GCTTGAAGAA GAATTCCAAA 900 | | GAAAGGCCAA CATATCCAGA GCTTATGCAA CATCCGTTTT TCACCCTACA CGAATCCAAA 960 | | GCGACAGACG TGGCATCTTT TGTAAAATCA ATTCTTGGAG ACTAA 1005 |
|
| Domain Profile | S: 1 lekleelGaGnggvVekvlhkksgkilavKtirleikkeeqkqilrelevlkkssnspyi 60 | le + elG+G++gvVek++h +s++i+avK+ir++++++eqk++l++l++ +++ ++p++ | Q: 53 LEPIVELGRGAYGVVEKMRHVPSEQIMAVKRIRATVNSQEQKRLLMDLDISMRTVDCPFT 112 | 567899****************************************************** |
| S: 61 vkfyGafvsegdvsicmelmdgtsldkvlkkvlekkkripekvlgkiavavvkaleylke 120 | v+fyGa+++egdv+icmelmd tsldk++k+v++k ++ipe++lgkiav++vkale+l++ | Q: 113 VTFYGALFREGDVWICMELMD-TSLDKFYKQVIDKGQTIPEDILGKIAVSIVKALEHLHS 171 | *********************.************************************** |
| S: 121 klkiiHRDvKPsNiLvnskGevKlCDFGvsgqlvdsiAksa.vGtksYmaPErieg...k 176 | kl++iHRDvKPsN+L+n+ G+vK+CDFG+sg+lvds+Ak++ +G+k+YmaPEri++ + | Q: 172 KLSVIHRDVKPSNVLINALGQVKMCDFGISGYLVDSVAKTIdAGCKPYMAPERINPelnQ 231 | ********************************************************9999 |
| S: 177 kkYsvksdvWslGltlvelatgrypyeklkeilellkaivneeppklpeeefseefqefv 236 | k Ysvksd+WslG+t++ela+ r+py+++ +++++lk++v+e++p+lp+++fseef++f | Q: 232 KGYSVKSDIWSLGITMIELAILRFPYDSWGTPFQQLKQVVEEPSPQLPADKFSEEFVDFT 291 | 9*********************************************************** |
| S: 237 skClkkdekeRpklkellkhafi 259 | s+Clkk++keRp++ el++h+f+ | Q: 292 SQCLKKNSKERPTYPELMQHPFF 314 | *********************96 |
|
Domain Sequence
(FASTA) | | LEPIVELGRG AYGVVEKMRH VPSEQIMAVK RIRATVNSQE QKRLLMDLDI SMRTVDCPFT 60 | | VTFYGALFRE GDVWICMELM DTSLDKFYKQ VIDKGQTIPE DILGKIAVSI VKALEHLHSK 120 | | LSVIHRDVKP SNVLINALGQ VKMCDFGISG YLVDSVAKTI DAGCKPYMAP ERINPELNQK 180 | | GYSVKSDIWS LGITMIELAI LRFPYDSWGT PFQQLKQVVE EPSPQLPADK FSEEFVDFTS 240 | | QCLKKNSKER PTYPELMQHP FF 262 |
|
| Keyword | Acetylation; Apoptosis; ATP-binding; Complete proteome; Cytoplasm; Cytoskeleton; Kinase; Nucleotide-binding; Nucleus; Phosphoprotein; Reference proteome; Serine/threonine-protein kinase; Stress response; Transcription; Transcription regulation; Transferase; Tyrosine-protein kinase. |
| Sequence Source | Ensembl |
| Orthology | |
| Gene Ontology | |
| KEGG | |
| InterPros | |
| Pfam | |
| SMARTs | |
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| Prints | |
| Created Date | 20-Feb-2013 |