EKS-BOT-00273

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EKS-BOT-00273

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
CMGC/DYRK	400.9	4.5E-120	688	1004	317

Status

Reviewed

Ensembl Protein

ENSBTAP00000002695

UniProt Accession

Q08DZ2;

Protein Name

Serine/threonine-protein kinase PRP4 homolog

Protein Synonyms/Alias

PRP4 pre-mRNA-processing factor 4 homolog;

Gene Name

PRPF4B

Gene Synonyms/Alias

PRPF4B;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
ENSBTAG00000002086	ENSBTAP00000002695	ENSBTAT00000002695

Organism

Bos taurus

Functional Description

Has a role in pre-mRNA splicing. Phosphorylates SF2/ASF(By similarity).

Protein Length

1008

Protein Sequence
(FASTA)

MAAAEAPSLR EQPEMEDANS EKSVNEENGE VSEDQSQNKH SRHKKKKHKH RSKHKKHKHS 60

SEEDKDRKHK HKHKHKKHKR KEVADASDKE GMSPAKRTKL DDLALLEDLE KQRALIKAEL 120

DNELMEGKVQ SGMGLILQGY ESGSEEEGEI HEKARNGNRS STRSSSTKGK LELVDNKNST 180

KKRSKSRSKE RTRHRSDKKK SKGGVEIVKE KATRSKSKER KKSKSPSKRS KSQDEARKSK 240

SPTLRRRSQE KVGKARSPVD DKAKVEDKSK AKDRKKSPVI NESRSRDRGK KSRSPVDLRG 300

KSKDRRSRSK ERKSKRPEAD KEKKPVKSPS KDASSGKENR SPSRRPGRSP KRRSLSPKQR 360

DKSRRSRSPL VNDRRSKQSK SPSRTLSPGR RAKSRSLERK RREPERRRLS SPRTRPRDDI 420

LSRRERSKDA SPISRWSPAR RRASRSPVRR RSRSPLRRSR SPRRRSRSPR RRDRGRRSRS 480

RLRRRSRSRG GRRRRSRSKV KEDKFKGSLS EGMKVEQESS SDDNLEDFDV EEEDEEALIE 540

QRRIQRQAIV QKYKYLADDS NLSVPSEPSS PQSSTRSRSP SPDDILERVA ADVKEYEREN 600

VDTFEASVKA KHNLMAVEQN NGSSQKKLLA PDMFTESDDM FAAYFDSARL RAAGIGKDFK 660

ENPNLRDNWT DAEGYYRVNI GEVLDKRYNV YGYTGQGVFS NVVRARDNAR ANQEVAVKII 720

RNNELMQKTG LKELEFLKKL NDADPDDKFH CLRLFRHFYH KQHLCLVFEP LSMNLREVLK 780

KYGKDVGLHI KAVRSYSQQL FLALKLLKRC NILHADIKPD NILVNESKTI LKLCDFGSAS 840

HVADNDITPY LVSRFYRAPE IIIGKSYDYG IDMWSVGCTL YELYTGKILF PGKTNNHMLK 900

LAMDLKGKMP NKMIRKGVFK DQHFDQNLNF MYIEVDKVTE REKVTVMSTI NPTKDLLADL 960

IGCQRLPEDQ RKKVHQLKDL LDQILMLDPA KRISINQALQ HAFIQEKI 1008

Nucleotide Sequence
(FASTA)

ATGGCCGCCG CGGAGGCGCC GTCGCTGCGG GAACAGCCGG AGATGGAAGA TGCTAATTCT 60

GAAAAGAGTG TTAATGAAGA AAACGGAGAA GTCTCGGAAG ACCAGTCTCA AAATAAGCAC 120

AGTCGTCACA AGAAAAAGAA GCATAAACAC AGAAGCAAAC ACAAGAAGCA CAAGCACTCC 180

TCGGAAGAAG ACAAGGACAG AAAGCATAAG CATAAGCACA AACATAAGAA ACACAAAAGG 240

AAGGAGGTCG CCGATGCTTC TGACAAGGAA GGTATGTCTC CAGCAAAAAG AACTAAACTT 300

GATGATTTAG CTCTGCTGGA AGACTTGGAG AAGCAGAGAG CCTTGATTAA AGCTGAACTC 360

GATAACGAGT TAATGGAAGG AAAGGTCCAG TCTGGGATGG GGCTCATATT ACAAGGTTAT 420

GAGTCCGGCT CTGAAGAAGA GGGGGAGATT CATGAAAAGG CAAGAAATGG AAATAGGTCT 480

AGTACTAGAT CTTCAAGTAC GAAGGGCAAA CTTGAACTTG TGGACAATAA AAATAGTACA 540

AAAAAGCGAA GCAAAAGCAG ATCCAAAGAA CGGACTAGAC ACAGGTCTGA TAAAAAGAAG 600

AGTAAGGGAG GTGTTGAAAT AGTTAAAGAG AAGGCAACAA GGAGCAAGTC AAAGGAGAGG 660

AAAAAGTCAA AAAGCCCCTC CAAAAGAAGT AAGTCCCAAG ATGAAGCCAG AAAGTCGAAG 720

TCGCCCACCC TCAGACGGCG CTCTCAAGAG AAAGTTGGGA AGGCCAGATC TCCTGTCGAC 780

GACAAGGCTA AAGTCGAAGA CAAAAGTAAG GCAAAGGACA GGAAGAAATC CCCTGTTATA 840

AACGAAAGTA GAAGTCGTGA TCGAGGCAAG AAATCTAGAT CCCCAGTCGA CTTGAGAGGC 900

AAATCCAAAG ACAGACGGTC ACGGTCCAAA GAGAGGAAAT CGAAACGGCC TGAAGCCGAC 960

AAAGAGAAGA AGCCGGTCAA GTCTCCCTCT AAAGACGCTT CCTCTGGGAA GGAAAACAGG 1020

TCACCCAGCA GAAGACCTGG TCGCAGTCCC AAAAGAAGAA GTCTGTCTCC GAAACAGCGG 1080

GACAAGTCCA GAAGAAGTCG ATCCCCACTC GTGAATGACA GGAGGTCTAA ACAGAGCAAG 1140

TCACCTTCTC GAACCCTGTC GCCTGGCAGG AGAGCCAAGA GCCGGTCCTT GGAAAGAAAA 1200

CGCAGGGAGC CCGAAAGGAG ACGCCTCTCT TCTCCAAGAA CACGACCTCG AGATGACATC 1260

CTCAGCAGGC GGGAAAGGTC CAAGGACGCC AGCCCGATCA GCAGGTGGTC TCCAGCTCGC 1320

AGACGGGCCA GCAGATCTCC TGTTAGGAGG AGGTCTCGTT CCCCTCTCAG ACGGAGCCGG 1380

TCTCCCAGAA GAAGAAGCAG GTCTCCTCGG AGAAGGGACA GAGGTCGGAG GAGCAGATCG 1440

CGCCTGAGAA GGCGGTCTCG ATCACGGGGT GGTCGTAGAC GGAGGAGCAG AAGCAAAGTA 1500

AAGGAAGACA AATTTAAAGG AAGTCTTTCT GAAGGAATGA AGGTCGAGCA GGAATCTTCA 1560

TCTGATGACA ACCTTGAAGA CTTCGATGTT GAGGAGGAAG ATGAAGAAGC CTTGATAGAA 1620

CAGAGAAGAA TACAAAGACA GGCCATCGTT CAGAAATACA AGTACCTTGC TGACGACAGC 1680

AACCTGTCGG TGCCGTCCGA GCCCAGCAGC CCACAGAGCA GCACCCGCTC CCGCTCCCCG 1740

TCTCCTGATG ACATCCTGGA GCGCGTGGCT GCCGATGTCA AGGAGTATGA GCGCGAGAAC 1800

GTCGACACGT TTGAGGCCTC CGTCAAAGCC AAGCATAATC TGATGGCCGT CGAGCAGAAT 1860

AACGGTTCAT CTCAGAAGAA GCTGTTGGCA CCTGATATGT TTACAGAATC TGATGATATG 1920

TTTGCTGCCT ATTTTGATAG TGCGCGTCTG CGGGCTGCTG GCATTGGGAA AGATTTCAAA 1980

GAGAATCCCA ACCTCAGGGA TAACTGGACT GATGCAGAAG GCTATTACCG TGTGAACATA 2040

GGTGAAGTCT TGGATAAGCG CTACAACGTG TACGGCTACA CGGGTCAAGG TGTGTTCAGT 2100

AACGTGGTCC GAGCCCGGGA TAACGCGAGG GCCAACCAGG AGGTGGCCGT GAAGATCATC 2160

AGGAACAACG AGCTCATGCA AAAAACTGGC TTAAAAGAAT TAGAATTCTT GAAAAAACTT 2220

AATGATGCTG ATCCTGATGA CAAGTTTCAT TGTTTGCGAC TCTTCAGACA TTTTTATCAC 2280

AAACAGCATC TGTGCCTGGT GTTTGAGCCT CTAAGTATGA ATTTACGAGA GGTGCTAAAA 2340

AAATACGGTA AGGATGTTGG CCTTCACATT AAGGCTGTCC GATCCTACAG TCAGCAGCTG 2400

TTCTTGGCTC TGAAACTCCT TAAAAGATGC AATATCCTCC ATGCAGATAT CAAGCCAGAC 2460

AATATCCTGG TTAATGAATC AAAAACTATA TTAAAGCTCT GCGACTTTGG GTCAGCCTCA 2520

CATGTTGCAG ATAACGACAT AACGCCTTAC CTTGTCAGTA GGTTTTACCG CGCTCCTGAG 2580

ATCATTATAG GTAAAAGCTA TGATTATGGT ATAGACATGT GGTCTGTAGG TTGCACCTTA 2640

TATGAACTCT ATACTGGAAA AATTTTATTT CCTGGCAAAA CCAATAACCA TATGTTGAAG 2700

CTCGCCATGG ATCTCAAAGG AAAGATGCCA AATAAGATGA TTCGGAAAGG CGTATTTAAA 2760

GACCAACATT TTGATCAAAA CCTCAACTTC ATGTACATAG AAGTTGATAA AGTGACCGAG 2820

AGGGAGAAAG TTACTGTCAT GAGCACCATT AATCCAACTA AGGACCTGTT GGCTGACTTG 2880

ATTGGGTGCC AGCGACTTCC TGAAGACCAG CGTAAGAAAG TGCACCAGCT GAAGGACTTG 2940

CTGGACCAGA TCCTGATGCT GGACCCCGCT AAACGGATCA GCATCAACCA GGCCCTGCAG 3000

CATGCCTTCA TCCAGGAGAA GATTTAA 3027

Domain Profile

S: 1     yevlevigkGsfgqVvkaldk.ktkeivaikiiknkkrfarqaqeEveiLeklnkkdkee  59

         y+v+ ++g+G+f++Vv+a+d+ +++++va+kii+n++ +++++ +E+e+L+kln++d+++

Q: 688   YNVYGYTGQGVFSNVVRARDNaRANQEVAVKIIRNNELMQKTGLKELEFLKKLNDADPDD  747

         99******************9889************************************

S: 60    kynvvrllelfvfrnhlclvfellsqnLyellkknkfs.glslklvrklaqqilkaLklL  118

         k++++rl+++f++++hlclvfe ls+nL+e+lkk++++ gl++k+vr+++qq++ aLklL

Q: 748   KFHCLRLFRHFYHKQHLCLVFEPLSMNLREVLKKYGKDvGLHIKAVRSYSQQLFLALKLL  807

         ************************************999*********************

S: 119   kelkiiHaDlKPeNillvekkrsevkviDFGsssavsqkeiytyiqsrfYraPEiilglk  178

         k+++i+HaD+KP+Nil++e+k+  +k++DFGs+s+v++++i++y++srfYraPEii+g++

Q: 808   KRCNILHADIKPDNILVNESKTI-LKLCDFGSASHVADNDITPYLVSRFYRAPEIIIGKS  866

         *********************99.************************************

S: 179   ydeaiDmWSlGcvlaElftGkpLfpGenevdqlalivevlglppekllekakkakkfftk  238

         yd+ iDmWS+Gc+l+El+tGk+LfpG++++++l+l ++++g++p+k+++k+  ++++f++

Q: 867   YDYGIDMWSVGCTLYELYTGKILFPGKTNNHMLKLAMDLKGKMPNKMIRKGVFKDQHFDQ  926

         ************************************************************

S: 239   keapkyilktkksdrkkklvlnvkkaakkrkaplskelgealkekedkl.fkdllkrmle  297

         + +++yi ++k+++r+k++v+++++ +k+  a+l  +++ +++++++ + +kdll+++l+

Q: 927   NLNFMYIEVDKVTEREKVTVMSTINPTKDLLADLIGCQRLPEDQRKKVHqLKDLLDQILM  986

         ***********************************************999**********

S: 298   ldpakRitpkealkhpfv  315

         ldpakRi++++al+h f+

Q: 987   LDPAKRISINQALQHAFI  1004

         *****************7

Domain Sequence
(FASTA)

YNVYGYTGQG VFSNVVRARD NARANQEVAV KIIRNNELMQ KTGLKELEFL KKLNDADPDD 60

KFHCLRLFRH FYHKQHLCLV FEPLSMNLRE VLKKYGKDVG LHIKAVRSYS QQLFLALKLL 120

KRCNILHADI KPDNILVNES KTILKLCDFG SASHVADNDI TPYLVSRFYR APEIIIGKSY 180

DYGIDMWSVG CTLYELYTGK ILFPGKTNNH MLKLAMDLKG KMPNKMIRKG VFKDQHFDQN 240

LNFMYIEVDK VTEREKVTVM STINPTKDLL ADLIGCQRLP EDQRKKVHQL KDLLDQILML 300

DPAKRISINQ ALQHAFI 317

Keyword

Acetylation; ATP-binding; Complete proteome; Kinase; mRNA processing; mRNA splicing; Nucleotide-binding; Nucleus; Phosphoprotein; Reference proteome; Serine/threonine-protein kinase; Spliceosome; Transferase.

Sequence Source

Ensembl

Orthology

Ortholog group

Ailuropoda melanoleuca"; ?>Anolis carolinensis"; ?>Caenorhabditis elegans"; ?>Callithrix jacchus"; ?>Canis familiaris"; ?>Cavia porcellus"; ?>Ciona intestinalis"; ?>Ciona savignyi"; ?>Danio rerio"; ?>Drosophila melanogaster"; ?>Echinops telfairi"; ?>Equus caballus"; ?>Felis catus"; ?>Gallus gallus"; ?>Gasterosteus aculeatus"; ?>Gorilla gorilla"; ?>Homo sapiens"; ?>Ictidomys tridecemlineatus"; ?>Latimeria chalumnae"; ?>Loxodonta africana"; ?>Macaca mulatta"; ?>Meleagris gallopavo"; ?>Monodelphis domestica"; ?>Mus musculus"; ?>Mustela putorius furo"; ?>Myotis lucifugus"; ?>Nomascus leucogenys"; ?>Oreochromis niloticus"; ?>Ornithorhynchus anatinus"; ?>Oryctolagus cuniculus"; ?>Oryzias latipes"; ?>Otolemur garnettii"; ?>Pan troglodytes"; ?>Pelodiscus sinensis"; ?>Pongo abelii"; ?>Rattus norvegicus"; ?>Sarcophilus harrisii"; ?>Sus scrofa"; ?>Taeniopygia guttata"; ?>Takifugu rubripes"; ?>Tetraodon nigroviridis"; ?>Xenopus tropicalis"; ?>Xiphophorus maculatus"; ?>Schizosaccharomyces pombe"; ?>

Gene Ontology

GO:0071013	; C:catalytic step 2 spliceosome
GO:0005694	; C:chromosome
GO:0005524	; F:ATP binding
GO:0004674	; F:protein serine/threonine kinase activity
GO:0006397	; P:mRNA processing
GO:0008380	; P:RNA splicing

KEGG

bta:512236

;

InterPros

IPR011009	; Kinase-like_dom.
IPR000719	; Prot_kinase_cat_dom.
IPR002290	; Ser/Thr_dual-sp_kinase_dom.
IPR008271	; Ser/Thr_kinase_AS.

Pfam

PF00069

; Pkinase; 1.

SMARTs

SM00220

; S_TKc; 1.

Prosites

PS00107	; PROTEIN_KINASE_ATP; FALSE_NEG.
PS50011	; PROTEIN_KINASE_DOM; 1.
PS00108	; PROTEIN_KINASE_ST; 1.

Prints

Created Date

20-Feb-2013