EKS-BOT-00382

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EKS-BOT-00382

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
TKL/MLK	314.5	5.7E-94	36	283	248

Status

Reviewed

Ensembl Protein

ENSBTAP00000003401

UniProt Accession

A2VDU3;

Protein Name

Mitogen-activated protein kinase kinase kinase 7

Protein Synonyms/Alias

Gene Name

MAP3K7

Gene Synonyms/Alias

MAP3K7;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
ENSBTAG00000002625	ENSBTAP00000003401	ENSBTAT00000003401

Organism

Bos taurus

Functional Description

Serine/threonine kinase which acts as an essentialcomponent of the MAP kinase signal transduction pathway. Plays an important role in the cascades of cellular responses evoked by changes in the environment. Mediates signal transduction of TRAF6, various cytokines including interleukin-1 (IL-1), transforming growth factor-beta (TGFB), TGFB-related factors like BMP2 and BMP4, toll-like receptors (TLR), tumor necrosis factor receptor CD40 and B-cell receptor (BCR). Ceramides are also able to activate MAP3K7/TAK1. Once activated, acts as an upstream activator of the MKK/JNK signal transduction cascade and the p38 MAPK signal transduction cascade through the phosphorylation and activation of several MAP kinase kinases like MAP2K1/MEK1, MAP2K3/MKK3, MAP2K6/MKK6 and MAP2K7/MKK7. These MAP2Ks in turn activate p38 MAPKs, c-jun N-terminal kinases (JNKs) and I-kappa-B kinase complex (IKK). Both p38 MAPK and JNK pathways control the transcription factors activator protein-1 (AP-1), while nuclear factor-kappa B is activated by IKK. MAP3K7 activates also IKBKB and MAPK8/JNK1 in response to TRAF6 signaling and mediates BMP2- induced apoptosis. In osmotic stress signaling, plays a major role in the activation of MAPK8/JNK1, but not that of NF-kappa-B (By similarity).

Protein Length

579

Protein Sequence
(FASTA)

MSTASAASSS SSSSAGEMIE APSQVLNFEE IDYKEIEVEE VVGRGAFGVV CKAKWRAKDV 60

AIKQIESESE RKAFIVELRQ LSRVNHPNIV KLYGACLNPV CLVMEYAEGG SLYNVLHGAE 120

PLPYYTAAHA MSWCLQCSQG VAYLHSMQPK ALIHRDLKPP NLLLVAGGTV LKICDFGTAC 180

DIQTHMTNNK GSAAWMAPEV FEGSNYSEKC DVFSWGIILW EVITRRKPFD EIGGPAFRIM 240

WAVHNGTRPP LIKNLPKPIE SLMTRCWSKD PSQRPSMEEI VKIMTHLMRY FPGADEPLQY 300

PCQYSDEGQS NSATSTGSFM DITSTNTSNK SDTNMEQVPA TNDTIKRLES KLLKNQAKQQ 360

SESGRLSLGA SRGSSVESLP PTSEGKRMSA DMSEIEARIA ATTGNGQPRR RSIQDLTVTG 420

TDPGQVSSRS SSPSVRMITT SGPTSEKPAR SHPWTPDDST DTNGSDNSIP MAYLTLDHQL 480

QPLAPCPNSK ESMAVFEQHC KMAQEYMKVQ TEIALLLQRK QELVAELDQD EKDQQNTSRL 540

VQEHKKLLDE NKSLSTYYQQ CKKQLEVIRS QQQKRQGTS 579

Nucleotide Sequence
(FASTA)

ATGTCTACAG CCTCCGCCGC TTCTTCTTCT TCCTCGTCTT CCGCCGGTGA GATGATCGAA 60

GCCCCCTCCC AAGTCCTCAA CTTTGAAGAG ATAGACTACA AGGAGATCGA GGTGGAAGAG 120

GTTGTTGGAA GAGGAGCTTT TGGAGTAGTA TGCAAAGCCA AGTGGAGAGC AAAAGATGTT 180

GCCATTAAAC AAATAGAAAG TGAATCTGAG AGGAAAGCTT TTATTGTAGA GCTTCGACAG 240

TTATCGCGTG TGAACCATCC TAACATCGTA AAGCTATATG GAGCCTGCTT GAATCCAGTG 300

TGTCTTGTAA TGGAATATGC TGAAGGGGGC TCCTTGTATA ATGTGCTGCA TGGTGCTGAA 360

CCATTGCCAT ATTACACCGC TGCCCACGCA ATGAGTTGGT GTTTACAGTG TTCCCAAGGA 420

GTGGCTTATC TTCACAGCAT GCAGCCCAAA GCTCTAATCC ACAGGGACCT GAAACCACCA 480

AACTTGCTGC TGGTTGCAGG GGGGACAGTT TTAAAAATCT GTGATTTTGG TACAGCCTGT 540

GACATTCAGA CTCACATGAC CAACAATAAG GGGAGTGCTG CTTGGATGGC ACCTGAAGTT 600

TTTGAAGGTA GCAATTACAG TGAAAAATGT GATGTCTTCA GCTGGGGTAT TATCCTTTGG 660

GAAGTGATAA CACGTCGGAA ACCCTTTGAT GAAATTGGTG GCCCGGCTTT CCGTATCATG 720

TGGGCTGTTC ATAACGGTAC TCGACCACCA CTGATCAAAA ATTTACCTAA GCCCATTGAG 780

AGCCTGATGA CTCGTTGTTG GTCTAAAGAT CCTTCTCAGC GCCCTTCAAT GGAGGAAATT 840

GTGAAAATAA TGACTCACTT GATGCGGTAC TTTCCAGGAG CAGATGAGCC GTTACAATAT 900

CCTTGTCAGT ATTCAGATGA AGGACAGAGC AACTCTGCTA CCAGTACAGG CTCCTTCATG 960

GACATCACTT CTACAAACAC CAGTAATAAA AGTGACACTA ACATGGAACA AGTTCCTGCC 1020

ACAAATGATA CAATTAAACG CTTAGAATCA AAATTGTTGA AAAACCAGGC AAAACAACAG 1080

AGTGAATCTG GGCGTTTGAG TTTGGGAGCT TCTCGTGGGA GCAGCGTGGA GAGCTTGCCC 1140

CCAACCTCTG AAGGCAAGAG GATGAGTGCC GATATGTCTG AAATAGAAGC CAGGATTGCT 1200

GCAACCACAG GCAACGGGCA GCCAAGACGT AGATCCATCC AAGACTTGAC TGTTACTGGA 1260

ACAGATCCTG GTCAGGTGAG CAGTAGGTCA TCCAGTCCTA GCGTCAGAAT GATCACTACC 1320

TCAGGACCAA CCTCAGAAAA GCCAGCTCGC AGTCATCCAT GGACCCCTGA TGATTCCACA 1380

GATACCAATG GATCAGATAA TTCCATCCCA ATGGCATATC TTACACTGGA TCACCAACTA 1440

CAGCCTCTAG CACCATGCCC AAACTCCAAA GAATCTATGG CAGTGTTTGA ACAGCATTGT 1500

AAGATGGCAC AGGAATACAT GAAAGTCCAA ACAGAAATTG CATTGTTATT ACAGAGAAAG 1560

CAGGAACTAG TTGCAGAACT GGACCAGGAT GAAAAGGACC AGCAAAATAC ATCTCGTCTG 1620

GTGCAGGAAC ATAAAAAGCT TTTAGATGAA AACAAAAGCC TTTCAACTTA CTATCAGCAA 1680

TGCAAAAAAC AGCTAGAGGT CATCAGGAGT CAGCAGCAAA AACGACAAGG CACTTCATGA 1740

Domain Profile

S: 1     ieleeligsgafgkvykakwrkkevavKklreeaeekekleklereakllsklshpniie  60

         ie+ee++g+gafg v+kakwr+k+va+K++++e+e+    +++  e+++ls+++hpni++

Q: 36    IEVEEVVGRGAFGVVCKAKWRAKDVAIKQIESESER----KAFIVELRQLSRVNHPNIVK  91

         5789****************************9888....********************

S: 61    llGvcleepnlllvmeyveggsLsnvlhe.kskreidlevlvswalqiArGmaYLhseap  119

         l+G+cl++  ++lvmey+eggsL+nvlh+ +  + ++ ++++sw+lq+++G+aYLhs++p

Q: 92    LYGACLNP--VCLVMEYAEGGSLYNVLHGaEPLPYYTAAHAMSWCLQCSQGVAYLHSMQP  149

         *******9..89*****************778899*************************

S: 120   kkiihrdLksknvLlseekktlkicdFGtarelskstkmsaagtlawmaPEvlkeekvse  179

         k++ihrdLk++n+Ll +  ++lkicdFGta++++ +++++++g++awmaPEv++ +++se

Q: 150   KALIHRDLKPPNLLLVAGGTVLKICDFGTACDIQ-THMTNNKGSAAWMAPEVFEGSNYSE  208

         ********************************99.9************************

S: 180   ksDvwSygvvLWelltrevPykdlse..laiaykvalkglrlpipsscpeklkkLmkecw  237

         k+Dv+S+g++LWe++tr++P++++    ++i+++v ++g+r+p+++++p+++++Lm++cw

Q: 209   KCDVFSWGIILWEVITRRKPFDEIGGpaFRIMWAV-HNGTRPPLIKNLPKPIESLMTRCW  267

         **********************9987447889999.99**********************

S: 238   nedpekRpsfseivks  253

         ++dp++Rps++eivk+

Q: 268   SKDPSQRPSMEEIVKI  283

         *************986

Domain Sequence
(FASTA)

IEVEEVVGRG AFGVVCKAKW RAKDVAIKQI ESESERKAFI VELRQLSRVN HPNIVKLYGA 60

CLNPVCLVME YAEGGSLYNV LHGAEPLPYY TAAHAMSWCL QCSQGVAYLH SMQPKALIHR 120

DLKPPNLLLV AGGTVLKICD FGTACDIQTH MTNNKGSAAW MAPEVFEGSN YSEKCDVFSW 180

GIILWEVITR RKPFDEIGGP AFRIMWAVHN GTRPPLIKNL PKPIESLMTR CWSKDPSQRP 240

SMEEIVKI 248

Keyword

Apoptosis; ATP-binding; Cell membrane; Complete proteome; Cytoplasm; Kinase; Magnesium; Membrane; Metal-binding; Nucleotide-binding; Phosphoprotein; Reference proteome; Serine/threonine-protein kinase; Stress response; Transcription; Transcription regulation; Transferase; Ubl conjugation.

Sequence Source

Ensembl

Orthology

Ortholog group

Ailuropoda melanoleuca"; ?>Anolis carolinensis"; ?>Callithrix jacchus"; ?>Canis familiaris"; ?>Cavia porcellus"; ?>Ciona intestinalis"; ?>Ciona savignyi"; ?>Danio rerio"; ?>Drosophila melanogaster"; ?>Echinops telfairi"; ?>Equus caballus"; ?>Felis catus"; ?>Gadus morhua"; ?>Gallus gallus"; ?>Gasterosteus aculeatus"; ?>Gorilla gorilla"; ?>Homo sapiens"; ?>Ictidomys tridecemlineatus"; ?>Latimeria chalumnae"; ?>Loxodonta africana"; ?>Macaca mulatta"; ?>Meleagris gallopavo"; ?>Monodelphis domestica"; ?>Mus musculus"; ?>Mustela putorius furo"; ?>Myotis lucifugus"; ?>Nomascus leucogenys"; ?>Oreochromis niloticus"; ?>Ornithorhynchus anatinus"; ?>Oryctolagus cuniculus"; ?>Oryzias latipes"; ?>Otolemur garnettii"; ?>Pan troglodytes"; ?>Pelodiscus sinensis"; ?>Petromyzon marinus"; ?>Pongo abelii"; ?>Rattus norvegicus"; ?>Sarcophilus harrisii"; ?>Sus scrofa"; ?>Taeniopygia guttata"; ?>Takifugu rubripes"; ?>Tetraodon nigroviridis"; ?>Tursiops truncatus"; ?>Vicugna pacos"; ?>Xenopus tropicalis"; ?>Xiphophorus maculatus"; ?>

Gene Ontology

GO:0005671	; C:Ada2/Gcn5/Ada3 transcription activator complex
GO:0008385	; C:IkappaB kinase complex
GO:0005886	; C:plasma membrane
GO:0005524	; F:ATP binding
GO:0000287	; F:magnesium ion binding
GO:0004708	; F:MAP kinase kinase activity
GO:0004709	; F:MAP kinase kinase kinase activity
GO:0007250	; P:activation of NF-kappaB-inducing kinase activity
GO:0001525	; P:angiogenesis
GO:0006915	; P:apoptotic process
GO:0043966	; P:histone H3 acetylation
GO:0007252	; P:I-kappaB phosphorylation
GO:0007254	; P:JNK cascade
GO:0043066	; P:negative regulation of apoptotic process
GO:0001841	; P:neural tube formation
GO:0043123	; P:positive regulation of I-kappaB kinase/NF-kappaB cascade
GO:0032743	; P:positive regulation of interleukin-2 production
GO:0046330	; P:positive regulation of JNK cascade
GO:0043507	; P:positive regulation of JUN kinase activity
GO:0002726	; P:positive regulation of T cell cytokine production
GO:0006355	; P:regulation of transcription, DNA-dependent
GO:0006351	; P:transcription, DNA-dependent
GO:0007179	; P:transforming growth factor beta receptor signaling pathway

KEGG

bta:529146

;

InterPros

IPR011009	; Kinase-like_dom.
IPR017421	; MAPKKK7.
IPR000719	; Prot_kinase_cat_dom.
IPR017441	; Protein_kinase_ATP_BS.
IPR001245	; Ser-Thr/Tyr_kinase_cat_dom.
IPR008271	; Ser/Thr_kinase_AS.

Pfam

PF07714

; Pkinase_Tyr; 1.

SMARTs

Prosites

PS00107	; PROTEIN_KINASE_ATP; 1.
PS50011	; PROTEIN_KINASE_DOM; 1.
PS00108	; PROTEIN_KINASE_ST; 1.

Prints

PR00109

; TYRKINASE.

Created Date

20-Feb-2013