EKS-BOT-00402

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EKS-BOT-00402

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
TK/PDGFR	602.2	3.7E-181	590	925	336

Status

Reviewed

Ensembl Protein

ENSBTAP00000003498

UniProt Accession

P43481;

Protein Name

Mast/stem cell growth factor receptor Kit

Protein Synonyms/Alias

SCFR; Proto-oncogene c-Kit; Tyrosine-protein kinase Kit;

Gene Name

KIT

Gene Synonyms/Alias

KIT;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
ENSBTAG00000002699	ENSBTAP00000003498	ENSBTAT00000003498

Organism

Bos taurus

Functional Description

Tyrosine-protein kinase that acts as cell-surfacereceptor for the cytokine KITLG/SCF and plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. In response to KITLG/SCF binding, KIT can activate several signaling pathways. Phosphorylates PIK3R1, PLCG1, SH2B2/APS and CBL. Activates the AKT1 signaling pathway by phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase. Activated KIT also transmits signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. Promotes activation of STAT family members STAT1, STAT3, STAT5A and STAT5B. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. KIT signaling is modulated by protein phosphatases, and by rapid internalization and degradation of the receptor. Activated KIT promotes phosphorylation of the protein phosphatases PTPN6/SHP-1 and PTPRU, and of the transcription factors STAT1, STAT3, STAT5A and STAT5B. Promotes phosphorylation of PIK3R1, CBL, CRK (isoform Crk-II), LYN, MAPK1/ERK2 and/or MAPK3/ERK1, PLCG1, SRC and SHC1 (By similarity).

Protein Length

977

Protein Sequence
(FASTA)

MRGARGAWDF LFVLLLLLLV QTGSSQPSVS PGELSLPSIH PAKSELIVSV GDEIRLLCTD 60

PGFVKWTFEI LGQLSEKTNP EWITEKAEAT NTGNYTCTNK GGLSSSIYVF VRDPEKLFLI 120

DLPLYGKEEN DTLVRCPLTD PEVTNYSLTG CEGKPLPKDL TFVADPKAGI TIRNVKREYH 180

RLCLHCSANQ RGKSMLSKKF TLKVRAAIKA VPVVSVSKTS YLLREGEEFA VTCLIKDVSS 240

SVDSMWIKEN SQQTKAQTKK NSWHQGDFSY LRQERLTISS ARVNDSGVFM CYANNTFGSA 300

NVTTTLEVVD KGFINIFPMM NTTVFVNDGE NVDLVVEYEA YPKPVHRQWI YMNRTSTDKW 360

DDYPKSENES NIRYVNELHL TRLKGTEGGT YTFHVSNSDV NSSVTFNVYV NTKPEILTHD 420

RLVNGMLQCV AAGFPEPTID WYFCPGTEQR CSVPVGPVDV QIQNSSVSPF GKLVVYSTID 480

DSTFKHNGTV ECRAYNDVGK SSASFNFAFK GNSKEQIHAH TLFTPLLIGF VIAAGLMCIF 540

VMILTYKYLQ KPMYEVQWKV VEEINGNNYV YIDPTQLPYD HKWEFPRNRL SFGKTLGAGA 600

FGKVVEATAY GLIKSDAAMT VAVKMLKPSA HLTEREALMS ELKVLSYLGN HMNIVNLLGA 660

CTIGGPTLVI TEYCCYGDLL NFLRRKRDSF ICSKQEDHAE VALYKNLLHS KESSCNDSTN 720

EYMDMKPGVS YVVPTKADKR RSARIGSYIE RDVTPAIMED DELALDLEDL LSFSYQVAKG 780

MAFLASKNCI HRDLAARNIL LTHGRITKIC DFGLARDIKN DSNYVVKGNA RLPVKWMAPE 840

SIFNCVYTFE SDVWSYGIFL WELFSLGSSP YPGMPVDSKF YKMIKEGFRM LSPEHAPAEM 900

YDIMKTCWDA DPLKRPTFKQ IVQLIEKQIS ESTNHIYSNL ANCSPHRENP AVDHSVRINS 960

VGSSASSTQP LLVHEDV 977

Nucleotide Sequence
(FASTA)

ATGAGAGGCG CTCGCGGCGC CTGGGATTTC CTCTTCGTTC TGCTGCTCCT GCTCCTCGTC 60

CAGACAGGCT CTTCTCAGCC ATCTGTGAGT CCAGGGGAAC TGTCTCTACC ATCTATCCAC 120

CCAGCAAAAT CAGAGTTAAT TGTCAGCGTT GGCGACGAGA TTAGGCTGTT ATGCACCGAT 180

CCAGGATTTG TCAAGTGGAC TTTTGAGATC CTGGGTCAAC TGAGTGAGAA AACAAACCCG 240

GAATGGATCA CCGAGAAAGC AGAGGCCACA AATACAGGCA ATTACACGTG CACCAATAAA 300

GGCGGCTTGA GCAGTTCCAT TTATGTGTTT GTTAGAGACC CCGAGAAGCT TTTCCTGATT 360

GACCTTCCCT TGTACGGGAA AGAAGAAAAC GACACGCTGG TTCGCTGTCC CCTGACAGAC 420

CCCGAGGTGA CCAATTACTC TCTTACGGGG TGTGAGGGGA AACCTCTCCC CAAGGATTTG 480

ACATTTGTGG CCGACCCCAA GGCAGGTATC ACAATCAGAA ATGTGAAGCG CGAGTACCAT 540

CGGCTCTGTC TGCACTGCTC AGCGAATCAG AGGGGCAAGT CCATGCTGTC GAAGAAATTC 600

ACTCTGAAAG TGCGGGCAGC CATCAAAGCT GTGCCAGTTG TGTCTGTGTC CAAAACCAGC 660

TATCTTCTCA GGGAAGGAGA GGAATTTGCA GTGACATGCT TGATTAAAGA CGTGTCTAGT 720

TCCGTGGACT CTATGTGGAT AAAGGAAAAC AGCCAGCAGA CTAAAGCACA GACGAAGAAG 780

AATAGCTGGC ATCAGGGTGA CTTCAGTTAT CTCCGTCAGG AAAGGTTGAC TATCAGCTCA 840

GCAAGAGTGA ATGATTCCGG TGTGTTCATG TGTTACGCCA ATAACACTTT TGGATCAGCA 900

AATGTCACAA CAACCTTAGA AGTAGTAGAT AAAGGATTCA TTAATATCTT CCCTATGATG 960

AACACAACAG TATTTGTAAA TGATGGAGAG AATGTGGATC TGGTTGTTGA ATATGAGGCA 1020

TATCCCAAAC CTGTACACCG ACAATGGATA TATATGAACA GAACCTCCAC TGATAAGTGG 1080

GACGATTATC CTAAGTCTGA AAATGAAAGT AATATCAGAT ACGTAAATGA ACTTCATCTA 1140

ACCAGATTAA AAGGGACTGA AGGAGGCACT TACACATTTC ACGTGTCCAA TTCTGATGTC 1200

AATTCTTCCG TGACATTTAA TGTTTACGTG AACACAAAAC CAGAAATCCT GACGCATGAC 1260

AGGCTGGTGA ATGGCATGCT ACAGTGCGTG GCCGCAGGGT TCCCGGAGCC AACCATCGAT 1320

TGGTACTTTT GTCCAGGAAC TGAGCAGAGG TGTTCTGTTC CCGTTGGGCC AGTGGATGTA 1380

CAGATCCAAA ACTCATCTGT CTCACCATTT GGAAAACTAG TGGTTTATAG CACCATTGAT 1440

GACAGCACAT TCAAACACAA TGGGACGGTG GAGTGCAGGG CTTATAACGA TGTGGGCAAG 1500

AGTTCTGCCT CTTTTAACTT TGCATTTAAA GGTAACAGCA AAGAACAAAT CCATGCTCAC 1560

ACCCTGTTCA CGCCGTTGCT GATTGGTTTT GTGATCGCAG CTGGTTTAAT GTGTATCTTC 1620

GTGATGATTC TTACATACAA ATATTTGCAG AAACCCATGT ATGAAGTACA GTGGAAAGTT 1680

GTCGAGGAGA TAAATGGAAA CAATTATGTT TACATAGACC CAACACAACT TCCTTATGAT 1740

CACAAATGGG AGTTTCCCAG GAACAGGCTG AGTTTTGGGA AAACCCTGGG TGCTGGCGCC 1800

TTCGGGAAAG TTGTTGAGGC CACCGCTTAT GGCTTAATTA AATCAGATGC AGCCATGACT 1860

GTTGCTGTCA AGATGCTCAA ACCAAGCGCC CATTTAACCG AACGAGAAGC CCTAATGTCT 1920

GAACTCAAAG TCTTGAGTTA CCTCGGTAAT CATATGAATA TTGTGAATCT TCTGGGAGCG 1980

TGCACCATTG GAGGGCCCAC CCTGGTCATT ACAGAATATT GTTGCTATGG TGATCTTCTG 2040

AATTTTTTGA GAAGAAAACG TGATTCATTT ATTTGCTCAA AGCAGGAAGA TCACGCCGAA 2100

GTGGCGCTTT ATAAGAACCT TCTTCATTCA AAGGAGTCTT CCTGCAATGA TAGTACTAAT 2160

GAGTACATGG ACATGAAACC TGGAGTTTCT TATGTTGTAC CAACCAAGGC AGACAAGAGG 2220

AGATCTGCAA GAATAGGTTC ATACATAGAA AGAGACGTGA CTCCTGCTAT CATGGAAGAT 2280

GATGAGCTGG CCCTGGACCT GGAGGACTTG CTGAGCTTTT CTTACCAGGT GGCAAAAGGC 2340

ATGGCGTTCC TTGCCTCAAA GAATTGTATT CATAGAGACT TGGCAGCCAG AAATATCCTC 2400

CTTACTCATG GTCGAATCAC AAAGATTTGT GATTTTGGTC TAGCCAGAGA CATCAAGAAT 2460

GATTCTAATT ATGTGGTCAA AGGAAACGCT CGACTCCCTG TGAAGTGGAT GGCACCAGAG 2520

AGTATTTTCA ACTGTGTATA CACATTTGAA AGTGATGTCT GGTCCTATGG GATTTTTCTG 2580

TGGGAGCTGT TCTCTTTAGG AAGCAGCCCC TACCCTGGAA TGCCAGTCGA TTCTAAGTTC 2640

TACAAGATGA TCAAGGAAGG TTTCCGAATG CTCAGCCCCG AGCATGCACC TGCGGAAATG 2700

TATGACATCA TGAAGACCTG CTGGGATGCT GATCCCTTGA AAAGGCCAAC ATTTAAGCAG 2760

ATTGTGCAGC TGATTGAGAA GCAGATCTCA GAGAGCACCA ATCATATTTA TTCCAACTTA 2820

GCAAACTGCA GCCCCCACCG GGAGAACCCC GCCGTGGACC ATTCTGTGCG CATCAACTCT 2880

GTGGGCAGCA GCGCCTCCTC CACGCAGCCT CTGCTTGTCC ACGAAGATGT CTGA 2934

Domain Profile

S: 1     lelGkvlGsGafGkvveatayGlsksdavlkvavkmlkstarssekealmselkilshlG  60

         l++Gk+lG+GafGkvveatayGl+ksda+++vavkmlk++a+ +e+ealmselk+ls+lG

Q: 590   LSFGKTLGAGAFGKVVEATAYGLIKSDAAMTVAVKMLKPSAHLTEREALMSELKVLSYLG  649

         79**********************************************************

S: 61    ehenivnllGactkgGpiyviteyccyGdllnylrrkresflkkslekkkekeeeldska  120

         +h+nivnllGact gGp++viteyccyGdlln+lrrkr+sf+   ++k+++++e + +k+

Q: 650   NHMNIVNLLGACTIGGPTLVITEYCCYGDLLNFLRRKRDSFI---CSKQEDHAEVALYKN  706

         ******************************************...999999*99999999

S: 121   lladkslrsksslssesseeyvdlkkdvsdvssskadklasekkksalerevkaaleeed  180

         l     l+sk+s++ +s++ey+d+k++vs+v ++kadk++s+++ s++er+v++a++e+d

Q: 707   L-----LHSKESSCNDSTNEYMDMKPGVSYVVPTKADKRRSARIGSYIERDVTPAIMEDD  761

         9.....9*****************************************************

S: 181   sevleledllsfsyqvakGmeflaskncvhrdlaarnvllteGkvvkicdfGlardimkd  240

         + +l+ledllsfsyqvakGm+flasknc+hrdlaarn+llt+G+++kicdfGlardi++d

Q: 762   ELALDLEDLLSFSYQVAKGMAFLASKNCIHRDLAARNILLTHGRITKICDFGLARDIKND  821

         ************************************************************

S: 241   snyvvkGnarlpvkwmapesifdslytvksdvwsyGillweifslGstpypgllvdskfy  300

         snyvvkGnarlpvkwmapesif+++yt++sdvwsyGi+lwe+fslGs+pypg++vdskfy

Q: 822   SNYVVKGNARLPVKWMAPESIFNCVYTFESDVWSYGIFLWELFSLGSSPYPGMPVDSKFY  881

         ************************************************************

S: 301   klikeGyrmaqpayateeiyeimqkcweaepekrpsfkqlvell  344

         k+ikeG+rm +p++a++e+y+im++cw+a+p krp+fkq+v+l+

Q: 882   KMIKEGFRMLSPEHAPAEMYDIMKTCWDADPLKRPTFKQIVQLI  925

         *****************************************996

Domain Sequence
(FASTA)

LSFGKTLGAG AFGKVVEATA YGLIKSDAAM TVAVKMLKPS AHLTEREALM SELKVLSYLG 60

NHMNIVNLLG ACTIGGPTLV ITEYCCYGDL LNFLRRKRDS FICSKQEDHA EVALYKNLLH 120

SKESSCNDST NEYMDMKPGV SYVVPTKADK RRSARIGSYI ERDVTPAIME DDELALDLED 180

LLSFSYQVAK GMAFLASKNC IHRDLAARNI LLTHGRITKI CDFGLARDIK NDSNYVVKGN 240

ARLPVKWMAP ESIFNCVYTF ESDVWSYGIF LWELFSLGSS PYPGMPVDSK FYKMIKEGFR 300

MLSPEHAPAE MYDIMKTCWD ADPLKRPTFK QIVQLI 336

Keyword

ATP-binding; Cell membrane; Complete proteome; Disulfide bond; Glycoprotein; Immunoglobulin domain; Kinase; Magnesium; Membrane; Metal-binding; Nucleotide-binding; Phosphoprotein; Proto-oncogene; Receptor; Reference proteome; Repeat; Signal; Transferase; Transmembrane; Transmembrane helix; Tyrosine-protein kinase; Ubl conjugation.

Sequence Source

Ensembl

Orthology

Ortholog group

Ailuropoda melanoleuca"; ?>Anolis carolinensis"; ?>Callithrix jacchus"; ?>Canis familiaris"; ?>Cavia porcellus"; ?>Danio rerio"; ?>Equus caballus"; ?>Felis catus"; ?>Gallus gallus"; ?>Gasterosteus aculeatus"; ?>Gorilla gorilla"; ?>Homo sapiens"; ?>Ictidomys tridecemlineatus"; ?>Latimeria chalumnae"; ?>Loxodonta africana"; ?>Macaca mulatta"; ?>Meleagris gallopavo"; ?>Monodelphis domestica"; ?>Mus musculus"; ?>Mustela putorius furo"; ?>Myotis lucifugus"; ?>Nomascus leucogenys"; ?>Oreochromis niloticus"; ?>Ornithorhynchus anatinus"; ?>Oryctolagus cuniculus"; ?>Oryzias latipes"; ?>Otolemur garnettii"; ?>Pan troglodytes"; ?>Pelodiscus sinensis"; ?>Pongo abelii"; ?>Pteropus vampyrus"; ?>Rattus norvegicus"; ?>Sarcophilus harrisii"; ?>Sus scrofa"; ?>Taeniopygia guttata"; ?>Takifugu rubripes"; ?>Tetraodon nigroviridis"; ?>Xenopus tropicalis"; ?>Xiphophorus maculatus"; ?>

Gene Ontology

GO:0005737	; C:cytoplasm
GO:0009897	; C:external side of plasma membrane
GO:0005615	; C:extracellular space
GO:0016021	; C:integral to membrane
GO:0005634	; C:nucleus
GO:0005524	; F:ATP binding
GO:0019955	; F:cytokine binding
GO:0046872	; F:metal ion binding
GO:0005020	; F:stem cell factor receptor activity
GO:0004714	; F:transmembrane receptor protein tyrosine kinase activity
GO:0031532	; P:actin cytoskeleton reorganization
GO:0000187	; P:activation of MAPK activity
GO:0060326	; P:cell chemotaxis
GO:0097067	; P:cellular response to thyroid hormone stimulus
GO:0002371	; P:dendritic cell cytokine production
GO:0050910	; P:detection of mechanical stimulus involved in sensory perception of sound
GO:0048565	; P:digestive tract development
GO:0035162	; P:embryonic hemopoiesis
GO:0030218	; P:erythrocyte differentiation
GO:0038162	; P:erythropoietin-mediated signaling pathway
GO:0038093	; P:Fc receptor signaling pathway
GO:0035234	; P:germ cell programmed cell death
GO:0006687	; P:glycosphingolipid metabolic process
GO:0002327	; P:immature B cell differentiation
GO:0006954	; P:inflammatory response
GO:0007243	; P:intracellular protein kinase cascade
GO:0038109	; P:Kit signaling pathway
GO:0030032	; P:lamellipodium assembly
GO:0002320	; P:lymphoid progenitor cell differentiation
GO:0008584	; P:male gonad development
GO:0002551	; P:mast cell chemotaxis
GO:0032762	; P:mast cell cytokine production
GO:0043303	; P:mast cell degranulation
GO:0060374	; P:mast cell differentiation
GO:0035855	; P:megakaryocyte development
GO:0097326	; P:melanocyte adhesion
GO:0030318	; P:melanocyte differentiation
GO:0097324	; P:melanocyte migration
GO:0002318	; P:myeloid progenitor cell differentiation
GO:0043069	; P:negative regulation of programmed cell death
GO:0001541	; P:ovarian follicle development
GO:0018108	; P:peptidyl-tyrosine phosphorylation
GO:0008284	; P:positive regulation of cell proliferation
GO:0010628	; P:positive regulation of gene expression
GO:0043410	; P:positive regulation of MAPK cascade
GO:0051091	; P:positive regulation of sequence-specific DNA binding transcription factor activity
GO:0042511	; P:positive regulation of tyrosine phosphorylation of Stat1 protein
GO:0042517	; P:positive regulation of tyrosine phosphorylation of Stat3 protein
GO:0042523	; P:positive regulation of tyrosine phosphorylation of Stat5 protein
GO:0046777	; P:protein autophosphorylation
GO:0008360	; P:regulation of cell shape
GO:0048070	; P:regulation of developmental pigmentation
GO:0009314	; P:response to radiation
GO:0007286	; P:spermatid development
GO:0007283	; P:spermatogenesis
GO:0048863	; P:stem cell differentiation
GO:0030217	; P:T cell differentiation

KEGG

InterPros

IPR007110	; Ig-like.
IPR013783	; Ig-like_fold.
IPR013098	; Ig_I-set.
IPR003599	; Ig_sub.
IPR003598	; Ig_sub2.
IPR011009	; Kinase-like_dom.
IPR000719	; Prot_kinase_cat_dom.
IPR017441	; Protein_kinase_ATP_BS.
IPR001245	; Ser-Thr/Tyr_kinase_cat_dom.
IPR008266	; Tyr_kinase_AS.
IPR020635	; Tyr_kinase_cat_dom.
IPR016243	; Tyr_kinase_CSF1/PDGF_rcpt.
IPR001824	; Tyr_kinase_rcpt_3_CS.

Pfam

PF07679	; I-set; 1.
PF07714	; Pkinase_Tyr; 1.

SMARTs

SM00409	; IG; 2.
SM00408	; IGc2; 1.
SM00219	; TyrKc; 1.

Prosites

PS50835	; IG_LIKE; 2.
PS00107	; PROTEIN_KINASE_ATP; 1.
PS50011	; PROTEIN_KINASE_DOM; 1.
PS00109	; PROTEIN_KINASE_TYR; 1.
PS00240	; RECEPTOR_TYR_KIN_III; 1.

Prints

Created Date

20-Feb-2013