EKS-BOT-00070

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EKS-BOT-00070

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
CMGC/CDK	338.9	2.3E-101	6	295	290

Status

Reviewed

Ensembl Protein

ENSBTAP00000009420

UniProt Accession

Q32KY4;

Protein Name

Cyclin-dependent kinase 4

Protein Synonyms/Alias

Cell division protein kinase 4;

Gene Name

CDK4

Gene Synonyms/Alias

CDK4;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
ENSBTAG00000007160	ENSBTAP00000009420	ENSBTAT00000009420

Organism

Bos taurus

Functional Description

Ser/Thr-kinase component of cyclin D-CDK4 (DC) complexesthat phosphorylate and inhibit members of the retinoblastoma (RB) protein family including RB1 and regulate the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complexes and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also phosphorylates SMAD3 in a cell-cycle-dependent manner and represses its transcriptional activity. Component of the ternary complex, cyclin D/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex (By similarity).

Protein Length

303

Protein Sequence
(FASTA)

MATSRYEPVA EIGVGAYGTV YKARDPHSGH FVALKSVRVP NGGGAGGGLP ISTVREVALL 60

RRLEAFEHPN VVRLMDVCAT ARTDRETKVT LVFEHVDQDL RTYLDKAPPP GLPVETIKDL 120

MRQFLRGLDF LHANCIVHRD LKPENILVTS GGTVKLADFG LARIYSYQMA LTPVVVTLWY 180

RAPEVLLQST YATPVDMWSV GCIFAEMFRR KPLFCGNSEA DQLGKIFDLI GLPPEDDWPR 240

DVSLPRGAFS PRGPRPVQSV VPELEESGAQ LLLEMLTFNP HKRISAFRAL QHSYLHKAEG 300

DAE 303

Nucleotide Sequence
(FASTA)

ATGGCTACCT CCCGATATGA GCCAGTGGCT GAGATTGGTG TCGGTGCCTA TGGGACAGTG 60

TACAAGGCCC GTGATCCCCA CAGTGGCCAC TTTGTGGCCC TCAAGAGTGT AAGAGTCCCC 120

AATGGAGGAG GTGCTGGAGG GGGCCTGCCC ATCAGCACCG TTCGGGAGGT GGCCTTACTG 180

CGGCGTCTGG AGGCTTTTGA GCATCCCAAT GTTGTCAGGC TTATGGACGT CTGTGCAACA 240

GCCCGAACTG ACCGGGAGAC CAAAGTGACC CTGGTGTTTG AGCATGTGGA CCAAGATCTC 300

AGGACATATC TGGACAAGGC ACCCCCACCA GGCTTGCCAG TGGAGACCAT AAAAGATCTG 360

ATGCGCCAAT TTCTAAGAGG CCTGGATTTC CTTCATGCCA ACTGCATCGT TCACCGAGAC 420

CTGAAGCCAG AGAACATTCT GGTGACAAGT GGTGGGACAG TCAAGCTGGC TGACTTTGGC 480

CTGGCCAGAA TCTACAGCTA CCAGATGGCA CTTACACCTG TGGTTGTTAC ACTCTGGTAT 540

CGTGCTCCAG AAGTTCTTTT GCAGTCTACG TATGCAACAC CCGTGGACAT GTGGAGCGTT 600

GGCTGTATCT TTGCAGAGAT GTTTCGTCGA AAGCCTCTCT TCTGTGGAAA CTCTGAAGCT 660

GACCAGTTAG GCAAAATCTT TGACCTGATT GGACTGCCCC CAGAGGATGA CTGGCCCCGA 720

GATGTCTCTC TACCCCGAGG AGCCTTTTCC CCCCGAGGGC CCCGCCCAGT GCAGTCGGTG 780

GTCCCTGAGC TGGAGGAATC TGGAGCACAG CTGCTGCTGG AGATGCTGAC TTTTAACCCA 840

CACAAGCGAA TCTCTGCCTT CCGAGCCCTG CAGCACTCTT ATCTACACAA GGCAGAAGGT 900

GACGCAGAGT GA 912

Domain Profile

S: 1     yeklekigeGtygkvykakdketgevvAlKkirlekeke...gvpitalrEisllkel..  55

         ye +++ig G+yg+vyka+d ++g++vAlK++r+ +      g+pi+++rE++ll++l

Q: 6     YEPVAEIGVGAYGTVYKARDPHSGHFVALKSVRVPNGGGaggGLPISTVREVALLRRLea  65

         88899*****************************99874444***************988

S: 56    .khknivkLlevva....kdkkklyLvfefleqdLkkllkkkkkkklsleevkslllqll  110

          +h+n+v+L++v+a     +++k++Lvfe+++qdL ++l+k +  +l+ e++k l++q+l

Q: 66    fEHPNVVRLMDVCAtartDRETKVTLVFEHVDQDLRTYLDKAPPPGLPVETIKDLMRQFL  125

         89************99855569**************************************

S: 111   eglaylHsnkilHRDlKpqNlLiskegklklaDfGlarafssplktytkevvTlwYraPe  170

         +gl++lH+n i+HRDlKp+N+L+++ g++klaDfGlar +s ++  +t++vvTlwYraPe

Q: 126   RGLDFLHANCIVHRDLKPENILVTSGGTVKLADFGLARIYSYQMA-LTPVVVTLWYRAPE  184

         ******************************************999.**************

S: 171   lLlgakeYstavDiWsvgcilaelltrkplfqgkseidqlerifkllgtpsekvwpevsk  230

         +Ll+++ Y t vD+Wsvgci+ae+++rkplf g+se dql +if+l+g p e++wp+  +

Q: 185   VLLQST-YATPVDMWSVGCIFAEMFRRKPLFCGNSEADQLGKIFDLIGLPPEDDWPRDVS  243

         ***985.*****************************************************

S: 231   lpeykksfpkkkkkklkekvkklkekaldllekllaldpkkRisakealqhkyf  284

         lp+    f+++ ++ ++++v++l+e++ +ll ++l+++p+kRisa +alqh+y+

Q: 244   LPRGA--FSPRGPRPVQSVVPELEESGAQLLLEMLTFNPHKRISAFRALQHSYL  295

         ***65..9999*****************************************96

Domain Sequence
(FASTA)

YEPVAEIGVG AYGTVYKARD PHSGHFVALK SVRVPNGGGA GGGLPISTVR EVALLRRLEA 60

FEHPNVVRLM DVCATARTDR ETKVTLVFEH VDQDLRTYLD KAPPPGLPVE TIKDLMRQFL 120

RGLDFLHANC IVHRDLKPEN ILVTSGGTVK LADFGLARIY SYQMALTPVV VTLWYRAPEV 180

LLQSTYATPV DMWSVGCIFA EMFRRKPLFC GNSEADQLGK IFDLIGLPPE DDWPRDVSLP 240

RGAFSPRGPR PVQSVVPELE ESGAQLLLEM LTFNPHKRIS AFRALQHSYL 290

Keyword

Acetylation; ATP-binding; Cell cycle; Cell division; Complete proteome; Cytoplasm; Kinase; Membrane; Nucleotide-binding; Nucleus; Phosphoprotein; Proto-oncogene; Reference proteome; Serine/threonine-protein kinase; Transferase.

Sequence Source

Ensembl

Orthology

Ortholog group

Ailuropoda melanoleuca"; ?>Anolis carolinensis"; ?>Callithrix jacchus"; ?>Canis familiaris"; ?>Cavia porcellus"; ?>Danio rerio"; ?>Echinops telfairi"; ?>Equus caballus"; ?>Erinaceus europaeus"; ?>Felis catus"; ?>Gasterosteus aculeatus"; ?>Gorilla gorilla"; ?>Homo sapiens"; ?>Ictidomys tridecemlineatus"; ?>Latimeria chalumnae"; ?>Loxodonta africana"; ?>Macaca mulatta"; ?>Macropus eugenii"; ?>Microcebus murinus"; ?>Mus musculus"; ?>Mustela putorius furo"; ?>Myotis lucifugus"; ?>Nomascus leucogenys"; ?>Ochotona princeps"; ?>Oryctolagus cuniculus"; ?>Oryzias latipes"; ?>Otolemur garnettii"; ?>Pan troglodytes"; ?>Pongo abelii"; ?>Procavia capensis"; ?>Pteropus vampyrus"; ?>Rattus norvegicus"; ?>Sarcophilus harrisii"; ?>Sorex araneus"; ?>Sus scrofa"; ?>Takifugu rubripes"; ?>Tarsius syrichta"; ?>Tursiops truncatus"; ?>Xiphophorus maculatus"; ?>

Gene Ontology

GO:0005737	; C:cytoplasm
GO:0016020	; C:membrane
GO:0005634	; C:nucleus
GO:0005524	; F:ATP binding
GO:0004693	; F:cyclin-dependent protein kinase activity
GO:0051301	; P:cell division

KEGG

bta:510618

;

InterPros

IPR011009	; Kinase-like_dom.
IPR000719	; Prot_kinase_cat_dom.
IPR017441	; Protein_kinase_ATP_BS.
IPR002290	; Ser/Thr_dual-sp_kinase_dom.
IPR008271	; Ser/Thr_kinase_AS.

Pfam

PF00069

; Pkinase; 1.

SMARTs

SM00220

; S_TKc; 1.

Prosites

PS00107	; PROTEIN_KINASE_ATP; 1.
PS50011	; PROTEIN_KINASE_DOM; 1.
PS00108	; PROTEIN_KINASE_ST; 1.

Prints

Created Date

20-Feb-2013