EKS-BOT-00442

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EKS-BOT-00442

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
AGC/DMPK	470.4	1.8E-141	89	351	263

Status

Unreviewed

Ensembl Protein

ENSBTAP00000053818

UniProt Accession

F1MF50;

Protein Name

Protein Synonyms/Alias

Rho-associated protein kinase 1;

Gene Name

ROCK1

Gene Synonyms/Alias

ROCK1;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
ENSBTAG00000008403	ENSBTAP00000053818	ENSBTAT00000061061

Organism

Bos taurus

Functional Description

Protein Length

1367

Protein Sequence
(FASTA)

MGADIELAIE LDLAEIMVLP NYNEAEKSQG VKVVCKRESN RKKSPENLKE NIPSQQNFLY 60

KMKEENDVTT LYKDTINKIR DLRMKAEDYE VVKVIGRGAF GEVQLVRHKS TRKVYAMKLL 120

SKFEMIKRSD SAFFWEERDI MAFANSPWVV QLFYAFQDDR YLYMVMEYMP GGDLVNLMSN 180

YDVPEKWARF YTAEVVLALD AIHSMGFIHR DVKPDNMLLD KSGHLKLADF GTCMKMNKEG 240

MVRCDTAVGT PDYISPEVLK SQGGDGYYGR ECDWWSVGVF LYEMLVGDTP FYADSLVGTY 300

SKIMNHKNSL TFPDDNDISK EAKNLICAFL TDREVRLGRN GVEEIKRHLF FKNDQWAWET 360

LRDTVAPVVP DLSSDIDTSN FDDLEEDKGD EETFPIPKAF VGNQLPFVGF TYYSNRRYLS 420

SANPNDNRTS SNVDKSLQEN LQKTIYKLEE QLHNEMQLKD EMEQKCRTSN IKLDKIMKEL 480

DEEGNQRRNL ESTVSQIEKE KMLLQHRINE YQRKAEQENE KRRNVENEVS TLKDQLEDLK 540

KVSQNSQLAN EKLAQLQKQL EEANDLLRTE SDTAVRLRKS HTEMSKSITQ LESLNRELQE 600

RNRILENSKS QMDKDYYQLQ AVLEAERRDR GHDSEKIGDL QARITSLQEE VKHLKHNLER 660

VEGERKEAQD MLNHSEKEKN NLEIDLNYKL KSLQQRLEQE VNEHKVTKAR LTDKHQSIEE 720

AKSVAMCEME KKLKEERDAR EKAENRVVQI EKQCSMLDVD LKQSQQKLEH LIENKDRMED 780

EVKNLTLQLE QESNKRLLLQ NELKTQAFEA DNLKGLEKQM KQEINTLLEA KRLLEFELAQ 840

LTKQYRGNEG QMRELQDQLE AEQYFSTLYK TQVKELKEEI EEKNRENLKK IQELQSEKET 900

LATQLDLAET KAESEQLARG LLEEQYFELT QESKKAASRN RQEITDKDHT LSRLEETNSM 960

LTKDIELLRK ENEELTDKMR KAEEEYKLKK EEEINILKAA FEKNINTERT LKTQAVNKLA 1020

EIMNRKDFKI DRKKANTQDL RKKEKENRKL QLELNQEREK FNQMVVKHQK ELNEMQAQLV 1080

EECTHKNELQ MQLASKESDI EQLRAKLLDL SDSTSVASFP SADETDGNLP ESRIEGWLSI 1140

PNRGNIKRYG WKKQYVVVSS KKILFYNDEQ DKEQSNPSMV LDIDKLFHVR PVTQGDVYRA 1200

ETEEIPKIFQ ILYANEGECR KDLEVEPVQQ AEKTNFQNHK GHEFIPTLYH FPANCEACAK 1260

PLWHVFKPPP ALECRRCHVK CHRDHIDKKE DLISPCKVSY DVTSARDMLL LACSQDEQKK 1320

WVTHLVKKIP KNPPSGFVRA SPRTLSTRST ANQSFRKVVK NTSGKAS 1367

Nucleotide Sequence
(FASTA)

ATGGGTGCTG ACATCGAGTT AGCTATAGAG TTAGATTTAG CTGAAATTAT GGTTTTGCCA 60

AACTATAATG AGGCAGAAAA GAGCCAGGGT GTTAAAGTTG TATGCAAGCG AGAAAGTAAC 120

AGGAAAAAAA GCCCAGAAAA TTTAAAAGAG AATATCCCAT CACAGCAGAA TTTTCTTTAC 180

AAAATGAAGG AAGAAAATGA TGTTACGACC TTATATAAAG ACACAATAAA TAAAATTAGA 240

GATTTACGAA TGAAAGCTGA AGATTATGAA GTAGTGAAGG TGATTGGTAG AGGTGCATTT 300

GGAGAAGTTC AATTGGTAAG GCATAAATCC ACCAGGAAGG TGTACGCTAT GAAGCTCCTC 360

AGCAAATTTG AAATGATTAA GAGATCTGAT TCTGCTTTTT TCTGGGAAGA AAGGGACATC 420

ATGGCTTTTG CTAACAGTCC TTGGGTTGTT CAGCTGTTTT ATGCATTCCA AGATGATCGT 480

TATCTTTACA TGGTAATGGA GTACATGCCT GGTGGAGATC TTGTAAATTT AATGAGCAAC 540

TATGACGTGC CTGAAAAATG GGCCCGATTC TATACTGCAG AAGTAGTTCT TGCATTGGAT 600

GCAATCCATT CCATGGGTTT TATCCATAGA GATGTGAAGC CTGATAACAT GCTGCTGGAT 660

AAATCTGGAC ATTTGAAGTT AGCAGATTTT GGTACTTGTA TGAAGATGAA TAAGGAAGGC 720

ATGGTACGAT GTGATACAGC AGTTGGAACA CCTGATTATA TTTCCCCTGA AGTATTAAAA 780

TCTCAAGGTG GCGATGGTTA TTATGGACGA GAATGTGACT GGTGGTCAGT TGGGGTATTT 840

TTATATGAAA TGCTTGTAGG TGATACACCA TTTTATGCAG ATTCTCTGGT TGGAACTTAC 900

AGTAAGATTA TGAACCATAA AAATTCTCTT ACCTTCCCTG ATGATAATGA CATATCAAAA 960

GAAGCAAAAA ATCTTATTTG TGCCTTCCTT ACTGACAGAG AGGTGAGATT AGGGCGAAAT 1020

GGTGTAGAAG AAATCAAACG ACATCTCTTC TTCAAAAATG ATCAATGGGC TTGGGAAACA 1080

CTTCGAGACA CTGTAGCACC AGTTGTACCT GATTTAAGTA GTGACATAGA CACAAGTAAC 1140

TTTGATGACT TGGAAGAAGA TAAAGGAGAT GAAGAAACAT TCCCTATACC TAAAGCTTTT 1200

GTTGGCAATC AACTACCTTT TGTAGGATTT ACATATTATA GCAATCGTAG ATACTTATCT 1260

TCAGCAAATC CTAACGATAA CAGAACTAGC TCTAATGTGG ATAAAAGCTT GCAGGAAAAT 1320

TTGCAGAAAA CAATTTATAA GCTGGAAGAA CAGTTACATA ATGAAATGCA ATTAAAAGAT 1380

GAAATGGAGC AGAAGTGCAG AACGTCAAAC ATAAAACTAG ACAAGATAAT GAAAGAATTG 1440

GACGAAGAGG GGAATCAAAG AAGAAATCTA GAATCTACAG TATCTCAGAT TGAGAAGGAG 1500

AAAATGTTGC TACAGCACAG AATTAATGAA TACCAAAGAA AAGCAGAACA GGAAAATGAG 1560

AAGAGGAGGA ATGTAGAAAA TGAAGTTTCT ACATTAAAGG ATCAGTTGGA AGACTTAAAG 1620

AAAGTCAGCC AGAATTCACA GCTTGCTAAT GAGAAGCTGG CACAATTACA AAAGCAGCTA 1680

GAAGAAGCCA ATGACTTACT TAGGACAGAA TCAGACACAG CGGTTAGATT AAGGAAGAGT 1740

CACACAGAGA TGAGCAAATC CATCACTCAG CTAGAGTCCC TAAACAGAGA ACTGCAGGAG 1800

AGAAATAGAA TTTTAGAGAA TTCCAAGTCA CAAATGGACA AAGATTATTA CCAACTACAG 1860

GCTGTATTAG AAGCTGAACG AAGAGACAGA GGTCATGATT CTGAGAAGAT TGGAGACCTA 1920

CAAGCTCGAA TTACATCTTT ACAAGAGGAG GTGAAGCATC TCAAACATAA TCTTGAAAGA 1980

GTGGAAGGAG AAAGAAAAGA GGCTCAAGAC ATGCTTAATC ATTCAGAAAA GGAAAAGAAT 2040

AACTTAGAAA TAGATTTAAA CTACAAGCTT AAATCATTAC AGCAACGATT AGAACAAGAG 2100

GTAAATGAAC ATAAAGTAAC CAAAGCTCGT TTAACTGACA AACATCAATC TATTGAAGAG 2160

GCAAAGTCTG TTGCAATGTG TGAGATGGAA AAAAAGCTGA AGGAGGAGAG AGACGCTCGA 2220

GAGAAGGCTG AAAATAGGGT TGTTCAGATT GAGAAGCAGT GCTCTATGCT AGATGTTGAT 2280

CTGAAGCAAT CTCAGCAGAA GCTGGAGCAT TTGATTGAAA ATAAAGACAG GATGGAGGAT 2340

GAAGTTAAGA ATCTAACGCT GCAGCTGGAG CAGGAATCAA ACAAGCGACT TTTGTTACAA 2400

AATGAATTGA AGACTCAAGC ATTTGAGGCA GACAATTTAA AAGGTTTAGA AAAGCAGATG 2460

AAACAGGAAA TAAATACTTT ATTGGAAGCA AAGAGATTGT TGGAATTTGA ATTAGCTCAG 2520

CTTACAAAAC AGTACAGAGG GAATGAAGGG CAGATGCGGG AGCTGCAGGA TCAGCTGGAA 2580

GCGGAGCAGT ATTTCTCGAC ACTTTATAAA ACCCAGGTAA AGGAACTTAA GGAAGAAATT 2640

GAAGAAAAAA ACAGAGAAAA CTTAAAGAAA ATACAGGAAC TACAAAGTGA AAAAGAAACT 2700

CTTGCTACTC AGTTGGATCT AGCAGAAACA AAGGCTGAAT CTGAGCAGTT GGCTCGAGGG 2760

CTTCTAGAAG AACAGTATTT TGAACTGACC CAAGAAAGCA AGAAAGCTGC CTCAAGAAAT 2820

AGACAAGAGA TTACAGATAA AGATCACACT CTTAGTCGGC TTGAAGAAAC GAATAGCATG 2880

CTAACCAAAG ATATTGAATT ATTAAGAAAA GAAAACGAAG AGCTAACTGA TAAAATGAGG 2940

AAGGCAGAGG AAGAATATAA ATTGAAGAAG GAGGAGGAGA TCAATATCCT TAAGGCTGCC 3000

TTTGAAAAGA ATATCAATAC AGAACGAACC CTTAAAACAC AGGCTGTTAA CAAGTTGGCA 3060

GAAATAATGA ATCGAAAGGA CTTTAAAATT GATAGAAAGA AAGCAAATAC GCAGGATTTG 3120

AGAAAGAAAG AAAAAGAAAA TCGAAAGCTC CAACTTGAAC TCAACCAGGA AAGAGAGAAA 3180

TTCAACCAAA TGGTAGTGAA ACATCAGAAG GAGCTGAATG AAATGCAAGC GCAATTGGTA 3240

GAAGAATGTA CACATAAGAA TGAGCTTCAG ATGCAGCTGG CCAGCAAGGA GAGTGACATT 3300

GAGCAGCTGC GTGCCAAACT GTTGGACCTT TCAGATTCTA CAAGTGTTGC TAGTTTTCCC 3360

AGTGCTGATG AAACTGATGG AAACCTTCCA GAATCAAGAA TTGAAGGTTG GCTTTCAATA 3420

CCAAATAGAG GAAATATCAA AAGATATGGC TGGAAGAAAC AGTATGTTGT GGTAAGCAGC 3480

AAAAAAATTT TGTTCTATAA TGATGAACAA GATAAGGAAC AATCCAATCC GTCTATGGTA 3540

TTGGACATTG ATAAATTGTT TCATGTTCGT CCTGTAACAC AAGGAGATGT ATACAGAGCT 3600

GAAACTGAAG AAATTCCTAA AATATTCCAG ATACTGTATG CAAATGAAGG TGAATGTAGA 3660

AAAGACTTAG AGGTGGAACC AGTACAACAA GCTGAAAAAA CAAATTTTCA AAACCACAAA 3720

GGCCATGAGT TCATTCCTAC ACTCTACCAC TTTCCCGCCA ACTGTGAGGC CTGTGCCAAA 3780

CCTCTCTGGC ATGTTTTTAA GCCACCCCCT GCCCTGGAGT GTCGAAGATG CCATGTTAAG 3840

TGCCACAGAG ACCACATAGA TAAGAAAGAG GACTTGATTT CTCCATGTAA AGTAAGTTAT 3900

GATGTAACAT CAGCAAGAGA TATGCTGCTG CTAGCGTGTT CTCAGGATGA ACAAAAAAAA 3960

TGGGTAACTC ATTTAGTAAA GAAGATCCCT AAGAATCCAC CATCTGGTTT TGTGCGTGCA 4020

TCCCCTCGAA CACTTTCTAC AAGGTCCACT GCAAATCAGT CTTTCCGGAA AGTGGTCAAA 4080

AATACATCTG GAAAAGCTAG TTAA 4104

Domain Profile

S: 1     feilkviGrGafgevavvrekatekvyamkilkklellkraesaffreerdvlvkadsew  60

         +e++kviGrGafgev++vr+k+t+kvyamk+l+k+e++kr++saff+eerd++++a+s+w

Q: 89    YEVVKVIGRGAFGEVQLVRHKSTRKVYAMKLLSKFEMIKRSDSAFFWEERDIMAFANSPW  148

         89**********************************************************

S: 61    ivqlqyafqdekylylvmeylvGGdlltllskyedklpeelarfylaelvlaldsvhelg  120

         +vql+yafqd++yly+vmey++GGdl++l+s+y+  +pe++arfy+ae+vlald++h++g

Q: 149   VVQLFYAFQDDRYLYMVMEYMPGGDLVNLMSNYD--VPEKWARFYTAEVVLALDAIHSMG  206

         **********************************..9***********************

S: 121   yvhrdikpdnilldksGhikladfGsclklnedgtvkssvavGtpdyispevlkaleegk  180

         ++hrd+kpdn+lldksGh+kladfG+c+k+n++g+v++++avGtpdyispevlk+++ g+

Q: 207   FIHRDVKPDNMLLDKSGHLKLADFGTCMKMNKEGMVRCDTAVGTPDYISPEVLKSQG-GD  265

         ********************************************************9.**

S: 181   geygkecdwwslGvilyellvgetpfyaeslvetyskilnhkeslkfpedekvseeakdl  240

         g+yg+ecdwws+Gv+lye+lvg+tpfya+slv tyski+nhk+sl+fp+d+++s+eak+l

Q: 266   GYYGRECDWWSVGVFLYEMLVGDTPFYADSLVGTYSKIMNHKNSLTFPDDNDISKEAKNL  325

         ************************************************************

S: 241   ikklltsreerlgregiedlkkhpff  266

         i+++lt+re rlgr+g+e++k+h ff

Q: 326   ICAFLTDREVRLGRNGVEEIKRHLFF  351

         *************************9

Domain Sequence
(FASTA)

YEVVKVIGRG AFGEVQLVRH KSTRKVYAMK LLSKFEMIKR SDSAFFWEER DIMAFANSPW 60

VVQLFYAFQD DRYLYMVMEY MPGGDLVNLM SNYDVPEKWA RFYTAEVVLA LDAIHSMGFI 120

HRDVKPDNML LDKSGHLKLA DFGTCMKMNK EGMVRCDTAV GTPDYISPEV LKSQGGDGYY 180

GRECDWWSVG VFLYEMLVGD TPFYADSLVG TYSKIMNHKN SLTFPDDNDI SKEAKNLICA 240

FLTDREVRLG RNGVEEIKRH LFF 263

Keyword

ATP-binding; Complete proteome; Kinase; Metal-binding; Nucleotide-binding; Reference proteome; Serine/threonine-protein kinase; Transferase; Zinc; Zinc-finger.

Sequence Source

Ensembl

Orthology

Ortholog group

Ailuropoda melanoleuca"; ?>Anolis carolinensis"; ?>Callithrix jacchus"; ?>Canis familiaris"; ?>Cavia porcellus"; ?>Danio rerio"; ?>Equus caballus"; ?>Felis catus"; ?>Gadus morhua"; ?>Gasterosteus aculeatus"; ?>Gorilla gorilla"; ?>Homo sapiens"; ?>Latimeria chalumnae"; ?>Loxodonta africana"; ?>Macaca mulatta"; ?>Meleagris gallopavo"; ?>Monodelphis domestica"; ?>Mus musculus"; ?>Mustela putorius furo"; ?>Myotis lucifugus"; ?>Nomascus leucogenys"; ?>Oreochromis niloticus"; ?>Oryzias latipes"; ?>Otolemur garnettii"; ?>Pan troglodytes"; ?>Pelodiscus sinensis"; ?>Pongo abelii"; ?>Rattus norvegicus"; ?>Sus scrofa"; ?>Taeniopygia guttata"; ?>Takifugu rubripes"; ?>Tetraodon nigroviridis"; ?>Xenopus tropicalis"; ?>Xiphophorus maculatus"; ?>

Gene Ontology

GO:0005622	; C:intracellular
GO:0005524	; F:ATP binding
GO:0046872	; F:metal ion binding
GO:0005543	; F:phospholipid binding
GO:0004674	; F:protein serine/threonine kinase activity
GO:0030036	; P:actin cytoskeleton organization
GO:0003383	; P:apical constriction
GO:0032060	; P:bleb assembly
GO:0000910	; P:cytokinesis
GO:0035556	; P:intracellular signal transduction
GO:0050901	; P:leukocyte tethering or rolling
GO:0022614	; P:membrane to membrane docking
GO:0016525	; P:negative regulation of angiogenesis
GO:0043524	; P:negative regulation of neuron apoptotic process
GO:0032970	; P:regulation of actin filament-based process
GO:0045616	; P:regulation of keratinocyte differentiation

KEGG

bta:785911

;

InterPros

IPR000961	; AGC-kinase_C.
IPR011072	; HR1_rho-bd.
IPR011009	; Kinase-like_dom.
IPR011993	; PH_like_dom.
IPR017892	; Pkinase_C.
IPR001849	; Pleckstrin_homology.
IPR002219	; Prot_Kinase_C-like_PE/DAG-bd.
IPR000719	; Prot_kinase_cat_dom.
IPR017441	; Protein_kinase_ATP_BS.
IPR020684	; Rho-assoc_coiled-coil_kin.
IPR015008	; Rho-bd.
IPR002290	; Ser/Thr_dual-sp_kinase_dom.
IPR008271	; Ser/Thr_kinase_AS.

Pfam

PF02185	; HR1; 1.
PF00169	; PH; 1.
PF00069	; Pkinase; 1.
PF00433	; Pkinase_C; 1.
PF08912	; Rho_Binding; 1.

SMARTs

SM00109	; C1; 1.
SM00233	; PH; 1.
SM00133	; S_TK_X; 1.
SM00220	; S_TKc; 1.

Prosites

PS51285	; AGC_KINASE_CTER; 1.
PS50003	; PH_DOMAIN; 1.
PS00107	; PROTEIN_KINASE_ATP; 1.
PS50011	; PROTEIN_KINASE_DOM; 1.
PS00108	; PROTEIN_KINASE_ST; 1.
PS50081	; ZF_DAG_PE_2; 1.

Prints

Created Date

20-Feb-2013