EKS-BOT-00118

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EKS-BOT-00118

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
Other/NAK	378.9	1.4E-113	1	254	254

Status

Reviewed

Ensembl Protein

ENSBTAP00000012302

UniProt Accession

F1MH24;

Protein Name

AP2-associated protein kinase 1

Protein Synonyms/Alias

Adaptor-associated kinase 1;

Gene Name

AAK1

Gene Synonyms/Alias

AAK1;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
ENSBTAG00000009343	ENSBTAP00000012302	ENSBTAT00000012302

Organism

Bos taurus

Functional Description

Regulates clathrin-mediated endocytosis byphosphorylating the AP2M1/mu2 subunit of the adaptor protein complex 2 (AP-2) which ensures high affinity binding of AP-2 to cargo membrane proteins during the initial stages of endocytosis. Regulates phosphorylation of other AP-2 subunits as well as AP-2 localization and AP-2-mediated internalization of ligand complexes. Phosphorylates NUMB and regulates its cellular localization, promoting NUMB localization to endosomes. Binds to and stabilizes the activated form of NOTCH1, increases its localization in endosomes and regulates its transcriptional activity (By similarity).

Protein Length

902

Protein Sequence
(FASTA)

GFAIVFLVRT SNGMKCALKR MFVNNEHDLQ VCKREIQIMR DLSGHKNIVG YIDSSINSVS 60

SGDVWEVLIL MDFCRGGQVV NLMNQRLQTG FTENEVLQIF CDTCEAVARL HQCKTPIIHR 120

DLKVENILLH DRGHYVLCDF GSATNKFQNP QTEGVNAVED EIKKYTTLSY RAPEMVNLYS 180

GKVITTKADI WALGCLLYKL CYFTLPFGES QVAICDGNFT IPDNSRYSQD MHCLIRYMLE 240

PDPDKRPDIY QVSYFSFKLL KKECPIPNVQ NSPIPTKLPE PVKASEAAAK KTQPKARLTD 300

PIPTTETSIA PRQRPKAGQT QPNPGILPIQ PALTPRKRAT VQPPPQAAGS SNQPGLLASV 360

PQPKTQPPPS QPLPQSQPKQ PQAPPTSQQP PSAPAQALPT QAQATPQHQQ QLFLKQQQQQ 420

QTAPPAQQPA GTFYQQPQQQ AQAPQFQAVH PAAQQPVIAQ FPVVSQGSSQ QQLIQNFYQQ 480

QQQQQQLATA LHQQQLLTQQ AALQQKTTAA AAPQPQAQPA AAASPAPAQE PAQIQAPVRQ 540

QPKVQTTPPP TIQGQKLGSL TPPSSPKAQR AGHRRILSDV THSAVFGVPA SKSTQLLQAA 600

AAEASLNKSK SATTTPSGSP RASQQNVYNP SEGSTWNPFD DDNFSKLTAE ELLNKDFAKL 660

GEGKYPEKLG GSAESLIPGF QPTQGDAFAA SSFSAGTAEK RKGGQTMDSS LPLLSVSDPF 720

IPLQVPDAPE KLIEGLKSPD TSLLLPDLLP MTDPFGSTSD AVIEKADVAV ESLIPGLEPP 780

VPQRLPSQTE SVTSNRTDSL TGEDSLIDCS LLSNPTTDLL EEFAPIAISA PAHKAAEDSN 840

LISGFDVPEG SDKVAEDEFD PIPVLITKNP QGGHSRNSSG SSESSLPNLA RSLLLVDQLI 900

DL 902

Nucleotide Sequence
(FASTA)

GGATTTGCCA TCGTGTTTCT GGTGAGGACG AGCAATGGAA TGAAATGTGC CTTGAAACGC 60

ATGTTTGTCA ACAACGAGCA TGATCTCCAG GTGTGCAAGA GAGAAATCCA GATAATGAGG 120

GACCTGTCGG GGCATAAGAA CATCGTGGGT TACATTGACT CCAGTATCAA TAGTGTGAGT 180

AGCGGTGATG TGTGGGAAGT TCTCATCCTG ATGGACTTCT GCAGGGGAGG CCAAGTGGTC 240

AACCTAATGA ACCAGCGCCT GCAAACAGGT TTTACAGAAA ATGAAGTACT CCAGATATTT 300

TGTGACACCT GTGAAGCTGT TGCCCGCCTG CATCAGTGCA AAACTCCTAT TATCCACCGT 360

GACCTGAAGG TTGAGAATAT TCTCTTGCAT GACCGAGGCC ACTATGTCCT GTGTGACTTT 420

GGAAGTGCTA CCAACAAATT CCAGAATCCA CAAACAGAAG GAGTCAATGC AGTAGAAGAT 480

GAGATTAAGA AGTACACAAC GCTGTCCTAT CGCGCACCAG AGATGGTCAA CCTGTACAGT 540

GGCAAGGTCA TCACGACGAA GGCAGACATT TGGGCTCTCG GATGTTTGTT GTATAAATTA 600

TGTTACTTCA CTTTGCCATT TGGGGAGAGT CAAGTGGCAA TTTGTGATGG AAACTTCACA 660

ATTCCTGATA ACTCTCGATA TTCCCAAGAC ATGCACTGCC TAATTAGGTA CATGTTGGAA 720

CCAGACCCTG ACAAAAGGCC GGATATTTAC CAGGTTTCCT ACTTCTCATT TAAACTACTC 780

AAGAAAGAAT GCCCAATTCC AAATGTACAG AACTCTCCCA TTCCTACAAA GCTCCCTGAA 840

CCAGTGAAAG CCAGTGAGGC AGCTGCAAAG AAGACCCAGC CAAAGGCCAG ACTGACAGAT 900

CCCATTCCCA CTACAGAGAC GTCAATTGCA CCCCGCCAGA GGCCTAAAGC TGGGCAGACT 960

CAGCCCAACC CAGGAATCCT CCCCATCCAA CCAGCCCTGA CCCCTCGGAA GAGGGCCACA 1020

GTTCAGCCCC CGCCTCAGGC CGCAGGATCC AGCAATCAGC CTGGTCTTTT AGCCAGTGTT 1080

CCTCAACCAA AAACCCAGCC CCCACCCAGT CAACCCCTAC CACAGTCTCA GCCCAAGCAG 1140

CCTCAGGCTC CGCCCACCTC ACAGCAGCCG CCTTCCGCGC CGGCCCAGGC TCTGCCCACC 1200

CAGGCCCAGG CCACGCCCCA GCACCAGCAG CAACTCTTCC TCAAGCAGCA GCAGCAGCAG 1260

CAGACAGCGC CGCCCGCACA GCAGCCAGCG GGCACGTTCT ACCAGCAGCC ACAACAGCAG 1320

GCCCAGGCTC CACAGTTTCA GGCAGTACAC CCAGCAGCCC AGCAACCAGT GATCGCTCAG 1380

TTCCCCGTGG TATCTCAAGG AAGCTCTCAA CAGCAGCTGA TACAGAACTT CTACCAGCAG 1440

CAGCAGCAGC AGCAGCAGCT GGCCACAGCC CTGCATCAGC AACAGCTGCT GACTCAGCAG 1500

GCTGCCTTGC AGCAGAAGAC GACAGCAGCG GCCGCACCGC AGCCCCAAGC ACAGCCGGCC 1560

GCAGCAGCCT CGCCAGCCCC TGCCCAGGAG CCAGCGCAGA TTCAAGCCCC AGTAAGACAA 1620

CAGCCAAAGG TTCAGACAAC CCCACCTCCT ACCATCCAGG GGCAGAAACT CGGATCTCTC 1680

ACTCCTCCAT CATCCCCCAA AGCCCAGCGT GCTGGGCACA GGCGAATTCT CAGCGACGTG 1740

ACCCACAGCG CAGTCTTTGG GGTCCCTGCC AGCAAATCAA CCCAGCTGCT CCAAGCAGCT 1800

GCAGCTGAGG CCAGTCTCAA TAAGTCCAAG TCTGCAACCA CCACTCCTTC AGGCTCCCCT 1860

CGGGCCTCCC AGCAAAATGT TTATAATCCT TCAGAAGGTT CTACCTGGAA TCCCTTTGAT 1920

GACGACAATT TCTCCAAACT GACAGCTGAA GAACTGCTAA ACAAGGACTT TGCCAAGCTG 1980

GGGGAAGGCA AGTATCCTGA GAAGCTTGGA GGCTCTGCTG AGAGTTTGAT CCCAGGCTTT 2040

CAACCAACCC AAGGTGATGC TTTTGCTGCT TCCTCATTTT CTGCTGGAAC TGCTGAAAAA 2100

AGGAAGGGTG GGCAGACTAT GGATTCTAGC CTTCCTCTTC TAAGTGTATC TGATCCTTTC 2160

ATTCCTCTTC AAGTACCTGA TGCACCAGAA AAACTAATTG AGGGACTCAA ATCTCCTGAC 2220

ACTTCTCTTC TGCTCCCTGA CCTCTTGCCT ATGACAGATC CTTTTGGTAG CACTTCTGAT 2280

GCTGTAATTG AAAAAGCTGA TGTTGCTGTT GAGAGTCTGA TACCAGGACT GGAGCCCCCG 2340

GTGCCTCAGC GCCTTCCATC TCAGACGGAA TCTGTGACCT CGAACCGCAC AGATTCTCTC 2400

ACCGGGGAAG ATTCCCTGAT TGATTGCTCT CTGCTTTCTA ACCCCACTAC TGACCTTCTG 2460

GAAGAGTTTG CCCCCATAGC CATCTCTGCT CCGGCCCATA AAGCTGCGGA AGATAGTAAT 2520

CTCATCTCAG GTTTTGATGT CCCTGAGGGC TCGGACAAGG TGGCAGAAGA TGAGTTTGAC 2580

CCTATTCCTG TATTGATAAC CAAAAACCCA CAAGGTGGGC ACTCTAGAAA CAGCAGTGGG 2640

AGCTCTGAGT CCAGTCTTCC CAACCTAGCC AGGTCTTTGC TGCTGGTGGA TCAGCTCATA 2700

GACCTGTAG 2709

Domain Profile

S: 11    GfalvylaedastgekyalKrilvneeedleeikrEieimkklsghknivklvdssvkee  70

         Gfa+v+l++ +s+g k+alKr++vn+e+dl+ +krEi+im++lsghkniv ++dss+++

Q: 1     GFAIVFLVR-TSNGMKCALKRMFVNNEHDLQVCKREIQIMRDLSGHKNIVGYIDSSINSV  59

         9********.99************************************************

S: 71    kdngskevlllleyckkgslvdllnkrlekgelseaeilkilldvtravaalHalsvpli  130

         ++++++evl+l+++c+ g++v+l+n+rl++g ++e+e+l+i++d+++ava+lH++++p+i

Q: 60    SSGDVWEVLILMDFCRGGQVVNLMNQRLQTG-FTENEVLQIFCDTCEAVARLHQCKTPII  118

         **9***************************9.****************************

S: 131   HRDlkveNillsdegelvlcDlGSattaileveskqelsalqeeieknttlsyrsPElid  190

         HRDlkveNill+d+g++vlcD+GSat+++++++ +++++a+++ei+k+ttlsyr+PE+++

Q: 119   HRDLKVENILLHDRGHYVLCDFGSATNKFQNPQ-TEGVNAVEDEIKKYTTLSYRAPEMVN  177

         *********************************.9*************************

S: 191   lysnlvideksDiWaLGcllYklcffesPfeesvklailngkysiPensryseklkslik  250

         lys++vi++k+DiWaLGcllYklc+f+ Pf+es ++ai++g+++iP+nsrys+++++li+

Q: 178   LYSGKVITTKADIWALGCLLYKLCYFTLPFGES-QVAICDGNFTIPDNSRYSQDMHCLIR  236

         *********************************.**************************

S: 251   kmlevdpeeRptiaqvle  268

         +mle+dp++Rp+i+qv++

Q: 237   YMLEPDPDKRPDIYQVSY  254

         ****************87

Domain Sequence
(FASTA)

GFAIVFLVRT SNGMKCALKR MFVNNEHDLQ VCKREIQIMR DLSGHKNIVG YIDSSINSVS 60

SGDVWEVLIL MDFCRGGQVV NLMNQRLQTG FTENEVLQIF CDTCEAVARL HQCKTPIIHR 120

DLKVENILLH DRGHYVLCDF GSATNKFQNP QTEGVNAVED EIKKYTTLSY RAPEMVNLYS 180

GKVITTKADI WALGCLLYKL CYFTLPFGES QVAICDGNFT IPDNSRYSQD MHCLIRYMLE 240

PDPDKRPDIY QVSY 254

Keyword

ATP-binding; Cell membrane; Coated pit; Complete proteome; Endocytosis; Kinase; Membrane; Nucleotide-binding; Phosphoprotein; Reference proteome; Serine/threonine-protein kinase; Transferase.

Sequence Source

Ensembl

Orthology

Ortholog group

Ailuropoda melanoleuca"; ?>Anolis carolinensis"; ?>Caenorhabditis elegans"; ?>Callithrix jacchus"; ?>Canis familiaris"; ?>Cavia porcellus"; ?>Ciona intestinalis"; ?>Ciona savignyi"; ?>Danio rerio"; ?>Drosophila melanogaster"; ?>Equus caballus"; ?>Felis catus"; ?>Gadus morhua"; ?>Gallus gallus"; ?>Gasterosteus aculeatus"; ?>Gorilla gorilla"; ?>Homo sapiens"; ?>Ictidomys tridecemlineatus"; ?>Latimeria chalumnae"; ?>Loxodonta africana"; ?>Macaca mulatta"; ?>Meleagris gallopavo"; ?>Monodelphis domestica"; ?>Mus musculus"; ?>Mustela putorius furo"; ?>Myotis lucifugus"; ?>Nomascus leucogenys"; ?>Oreochromis niloticus"; ?>Ornithorhynchus anatinus"; ?>Oryctolagus cuniculus"; ?>Oryzias latipes"; ?>Otolemur garnettii"; ?>Pan troglodytes"; ?>Pelodiscus sinensis"; ?>Petromyzon marinus"; ?>Pongo abelii"; ?>Rattus norvegicus"; ?>Sarcophilus harrisii"; ?>Sus scrofa"; ?>Taeniopygia guttata"; ?>Takifugu rubripes"; ?>Tetraodon nigroviridis"; ?>Xenopus tropicalis"; ?>Xiphophorus maculatus"; ?>Saccharomyces cerevisiae"; ?>Schizosaccharomyces pombe"; ?>

Gene Ontology

GO:0031252	; C:cell leading edge
GO:0030136	; C:clathrin-coated vesicle
GO:0005905	; C:coated pit
GO:0019897	; C:extrinsic to plasma membrane
GO:0043195	; C:terminal button
GO:0035612	; F:AP-2 adaptor complex binding
GO:0005524	; F:ATP binding
GO:0005112	; F:Notch binding
GO:0004674	; F:protein serine/threonine kinase activity
GO:0006897	; P:endocytosis
GO:0045747	; P:positive regulation of Notch signaling pathway
GO:0046777	; P:protein autophosphorylation
GO:0050821	; P:protein stabilization
GO:2000369	; P:regulation of clathrin-mediated endocytosis
GO:0032880	; P:regulation of protein localization

KEGG

InterPros

IPR011009	; Kinase-like_dom.
IPR000719	; Prot_kinase_cat_dom.
IPR008271	; Ser/Thr_kinase_AS.

Pfam

PF00069

; Pkinase; 1.

SMARTs

Prosites

PS00107	; PROTEIN_KINASE_ATP; FALSE_NEG.
PS50011	; PROTEIN_KINASE_DOM; 1.
PS00108	; PROTEIN_KINASE_ST; 1.

Prints

Created Date

20-Feb-2013