EKS-BOT-00450

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EKS-BOT-00450

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
Atypical/PDHK	185.6	4.2E-55	264	400	137

Status

Reviewed

Ensembl Protein

ENSBTAP00000013909

UniProt Accession

Q2KJG8;

Protein Name

[3-methyl-2-oxobutanoate dehydrogenase [lipoamide]] kinase, mitochondrial

Protein Synonyms/Alias

Branched-chain alpha-ketoacid dehydrogenase kinase; BCKD-kinase; BCKDHKIN;

Gene Name

BCKDK

Gene Synonyms/Alias

BCKDK;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
ENSBTAG00000010524	ENSBTAP00000013909	ENSBTAT00000013909

Organism

Bos taurus

Functional Description

Catalyzes the phosphorylation and inactivation of thebranched-chain alpha-ketoacid dehydrogenase complex, the key regulatory enzyme of the valine, leucine and isoleucine catabolic pathways. Key enzyme that regulate the activity state of the BCKD complex (By similarity).

Protein Length

412

Protein Sequence
(FASTA)

MILASVLGSG PRGGPPLRPL LGPALSLRAR STSATDTHHV EMARERSKTV TSFYNQSAID 60

VAAEKPSVRL TPTMMLYSGR SQDGSHLLKS ARYLQQELPV RIAHRIKGFR SLPFIIGCNP 120

TILHVHELYI RAFQKLTDFP PIKDQADEAR YCQLVRQLLD DHKDVVTLLA EGLRESRKYI 180

EDEKLVRYFL DKTLTSRLGI RMLATHHLAL HEDKPDFVGI ICTRLSPKKI IEKWVDFARR 240

LCEHKYGNAP RVRINGHVAA RFPFIPMPLD YILPELLKNA MRATMESHLD TPYNVPDVVI 300

TIANNDIDLV IRISDRGGGI AHKDLDRVMD YHFTTAEAST QDPRISPLFG HLDLHSGGQS 360

GPMHGFGFGL PTSRAYAEYL GGSLRLQSLQ GIGTDVYLRL RHIDGREESF RI 412

Nucleotide Sequence
(FASTA)

ATGATACTGG CTTCGGTGCT GGGGAGCGGA CCCCGGGGCG GGCCACCCCT CCGGCCTCTC 60

TTGGGGCCCG CACTCTCGCT CCGGGCCCGC TCCACATCAG CCACTGATAC CCACCATGTG 120

GAAATGGCAC GGGAGCGCTC TAAGACCGTC ACCTCCTTTT ACAACCAGTC AGCCATTGAC 180

GTCGCAGCGG AGAAGCCTTC AGTCCGCCTC ACTCCAACCA TGATGCTCTA TTCAGGCCGC 240

TCCCAGGATG GCAGTCACCT CCTGAAAAGT GCCCGCTACT TGCAGCAGGA GTTGCCAGTG 300

AGGATCGCTC ACCGCATCAA GGGCTTCCGC AGCCTTCCTT TCATCATTGG CTGCAACCCC 360

ACCATACTGC ACGTGCACGA GCTGTACATC CGTGCCTTCC AGAAGCTGAC AGACTTCCCT 420

CCGATCAAGG ACCAGGCGGA TGAGGCCCGA TACTGCCAGC TGGTGCGACA GCTGTTGGAT 480

GACCACAAGG ACGTGGTGAC CCTCTTAGCG GAAGGCCTGC GTGAGAGCCG GAAGTACATA 540

GAGGATGAGA AGCTCGTCCG TTACTTCTTA GACAAGACGT TGACTTCAAG GCTTGGGATC 600

CGTATGTTGG CCACCCACCA TCTGGCACTA CATGAGGACA AGCCCGACTT TGTTGGCATC 660

ATCTGCACTC GCCTGTCACC GAAGAAGATT ATTGAAAAGT GGGTGGACTT TGCCAGACGC 720

CTGTGTGAGC ACAAGTATGG CAATGCACCC CGAGTCCGCA TCAACGGACA CGTGGCTGCC 780

CGTTTCCCCT TCATCCCAAT GCCACTGGAC TACATCCTAC CTGAGCTGCT CAAGAACGCC 840

ATGAGAGCCA CAATGGAGAG TCACCTCGAC ACTCCCTACA ATGTCCCAGA TGTCGTCATC 900

ACCATCGCCA ACAATGATAT CGATCTCGTC ATCAGGATTT CAGACCGGGG CGGGGGAATT 960

GCTCACAAAG ACCTGGATCG GGTGATGGAC TACCACTTCA CTACAGCTGA GGCCAGCACC 1020

CAGGACCCCC GGATCAGCCC CCTCTTTGGC CACCTGGACT TGCACAGTGG TGGCCAGTCA 1080

GGACCCATGC ACGGCTTTGG CTTCGGGCTG CCCACTTCGC GGGCCTACGC AGAGTACCTC 1140

GGTGGTTCCC TTCGGCTGCA GTCGCTGCAG GGCATTGGCA CAGATGTCTA CCTACGGCTC 1200

CGTCACATCG ATGGCCGGGA AGAAAGCTTC CGCATCTGA 1239

Domain Profile

S: 1     yvpshlyyllfellknamratveshedkkv.lpdvkvlvalgkedliikisdrGGGvplr  59

         ++p++l+y+l+ellknamrat+esh d+++ +pdv++++a+++ dl+i+isdrGGG++++

Q: 264   FIPMPLDYILPELLKNAMRATMESHLDTPYnVPDVVITIANNDIDLVIRISDRGGGIAHK  323

         8*****************************9*****************************

S: 60    kidrlldymystapapskdd..............araaplaGfGyGlpisrlyakylqGd  105

         ++dr++dy+++ta+a+++d+               +++p++GfG+Glp+sr+ya+yl+G+

Q: 324   DLDRVMDYHFTTAEASTQDPrisplfghldlhsgGQSGPMHGFGFGLPTSRAYAEYLGGS  383

         *********************************99*************************

S: 106   lkllsleGiGtdaviylka  124

         l+l+sl+GiGtd+  yl++

Q: 384   LRLQSLQGIGTDV--YLRL  400

         ************9..8886

Domain Sequence
(FASTA)

FIPMPLDYIL PELLKNAMRA TMESHLDTPY NVPDVVITIA NNDIDLVIRI SDRGGGIAHK 60

DLDRVMDYHF TTAEASTQDP RISPLFGHLD LHSGGQSGPM HGFGFGLPTS RAYAEYLGGS 120

LRLQSLQGIG TDVYLRL 137

Keyword

Acetylation; ATP-binding; Complete proteome; Kinase; Mitochondrion; Nucleotide-binding; Phosphoprotein; Reference proteome; Serine/threonine-protein kinase; Transferase; Transit peptide.

Sequence Source

Ensembl

Orthology

Ortholog group

Ailuropoda melanoleuca"; ?>Anolis carolinensis"; ?>Callithrix jacchus"; ?>Canis familiaris"; ?>Cavia porcellus"; ?>Danio rerio"; ?>Dasypus novemcinctus"; ?>Dipodomys ordii"; ?>Echinops telfairi"; ?>Equus caballus"; ?>Felis catus"; ?>Gadus morhua"; ?>Gasterosteus aculeatus"; ?>Gorilla gorilla"; ?>Homo sapiens"; ?>Ictidomys tridecemlineatus"; ?>Latimeria chalumnae"; ?>Loxodonta africana"; ?>Macaca mulatta"; ?>Monodelphis domestica"; ?>Mus musculus"; ?>Mustela putorius furo"; ?>Myotis lucifugus"; ?>Nomascus leucogenys"; ?>Ochotona princeps"; ?>Oreochromis niloticus"; ?>Oryctolagus cuniculus"; ?>Oryzias latipes"; ?>Otolemur garnettii"; ?>Pan troglodytes"; ?>Pelodiscus sinensis"; ?>Pongo abelii"; ?>Procavia capensis"; ?>Pteropus vampyrus"; ?>Rattus norvegicus"; ?>Sarcophilus harrisii"; ?>Sus scrofa"; ?>Takifugu rubripes"; ?>Tetraodon nigroviridis"; ?>Xenopus tropicalis"; ?>Xiphophorus maculatus"; ?>

Gene Ontology

GO:0005947	; C:mitochondrial alpha-ketoglutarate dehydrogenase complex
GO:0047323	; F:[3-methyl-2-oxobutanoate dehydrogenase (acetyl-transferring)] kinase activity
GO:0005524	; F:ATP binding
GO:0004674	; F:protein serine/threonine kinase activity
GO:0009083	; P:branched-chain amino acid catabolic process

KEGG

bta:505005

;

InterPros

IPR003594	; ATPase-like_ATP-bd.
IPR018955	; BCDHK/PDK_N.
IPR004358	; Sig_transdc_His_kin-like_C.
IPR005467	; Sig_transdc_His_kinase_core.

Pfam

PF10436	; BCDHK_Adom3; 1.
PF02518	; HATPase_c; 1.

SMARTs

SM00387

; HATPase_c; 1.

Prosites

PS50109

; HIS_KIN; 1.

Prints

PR00344

; BCTRLSENSOR.

Created Date

20-Feb-2013