EPS-BOT-00133

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EPS-BOT-00133

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
PPP/PP2B	435.9	2.0E-131	84	284	201

Status

Reviewed

Ensembl Protein

ENSBTAP00000047848

UniProt Accession

P48452; Q08DM4; Q309F2;

Protein Name

Serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform

Protein Synonyms/Alias

CAM-PRP catalytic subunit; Calmodulin-dependent calcineurin A subunit alpha isoform;

Gene Name

PPP3CA

Gene Synonyms/Alias

PPP3CA;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
ENSBTAG00000016005	ENSBTAP00000021305	ENSBTAT00000021305
ENSBTAG00000016005	ENSBTAP00000047848	ENSBTAT00000056385

Organism

Bos taurus

Functional Description

Calcium-dependent, calmodulin-stimulated proteinphosphatase. This subunit may have a role in the calmodulin activation of calcineurin. Dephosphorylates DNM1L, HSPB1 and SSH1 (By similarity).

Protein Length

511

Protein Sequence
(FASTA)

MSEPKAIDPK LSTTDRVVKA VPFPPSHRLT AKEVFDNDGK PRVDILKAHL MKEGRLEETV 60

ALRIITEGAS ILRQEKNLLD IDAPVTVCGD IHGQFFDLMK LFEVGGSPAN TRYLFLGDYV 120

DRGYFSIECV LYLWALKILY PKTLFLLRGN HECRHLTEYF TFKQECKIKY SERVYDACMD 180

AFDCLPLAAL MNQQFLCVHG GLSPEINTLD DIRKLDRFKE PPAYGPMCDI LWSDPLEDFG 240

NEKTQEHFTH NTVRGCSYFY SYPAVCEFLQ HNNLLSILRA HEAQDAGYRM YRKSQTTGFP 300

SLITIFSAPN YLDVYNNKAA VLKYENNVMN IRQFNCSPHP YWLPNFMDVF TWSLPFVGEK 360

VTEMLVNVLN ICSDDELGSE EDGFDGATAA ARKEVIRNKI RAIGKMARVF SVLREESESV 420

LTLKGLTPTG MLPSGVLSGG KQTLQSAIKG FSPQHKITSF EEAKGLDRIN ERMPPRRDAM 480

PSDANLNSIN KALASETNGT DSNGSNSSNI Q 511

Nucleotide Sequence
(FASTA)

ATGTCCGAGC CCAAGGCAAT TGATCCCAAG TTGTCGACGA CCGACAGGGT GGTGAAAGCT 60

GTCCCATTTC CTCCAAGTCA CCGGCTTACG GCAAAAGAAG TGTTTGATAA TGATGGGAAA 120

CCTCGTGTGG ATATCTTAAA GGCACATCTC ATGAAGGAGG GCAGGCTCGA AGAGACTGTC 180

GCATTGAGAA TAATAACAGA GGGGGCTTCA ATTCTTCGAC AGGAAAAAAA CTTGCTGGAT 240

ATTGATGCTC CAGTCACAGT TTGTGGGGAC ATCCATGGAC AATTCTTTGA CTTGATGAAA 300

CTCTTTGAAG TGGGGGGATC TCCTGCCAAC ACTCGCTACC TCTTCTTAGG GGACTATGTT 360

GACAGAGGGT ACTTCAGTAT CGAATGTGTG CTGTATTTGT GGGCTTTGAA AATTCTTTAC 420

CCCAAAACAC TGTTTTTACT TCGTGGAAAT CATGAATGTA GACATCTAAC AGAGTATTTC 480

ACATTTAAAC AAGAATGTAA AATAAAGTAT TCAGAACGCG TATATGATGC CTGCATGGAT 540

GCCTTTGACT GCCTGCCCCT GGCTGCCCTG ATGAACCAGC AGTTCCTGTG TGTACACGGC 600

GGTTTGTCTC CAGAGATAAA CACTTTAGAT GATATCAGAA AATTAGACCG ATTCAAAGAA 660

CCACCTGCTT ATGGACCTAT GTGTGATATC CTGTGGTCAG ACCCGCTGGA AGATTTTGGA 720

AACGAGAAGA CTCAGGAACA TTTCACTCAC AACACAGTCA GGGGGTGTTC ATACTTCTAC 780

AGTTACCCGG CTGTATGTGA ATTCTTGCAG CACAATAACT TGTTATCTAT CCTCCGAGCT 840

CATGAAGCCC AAGATGCAGG GTACCGCATG TACAGGAAAA GCCAAACAAC AGGCTTCCCC 900

TCTCTCATTA CAATTTTTTC AGCACCAAAT TACTTAGATG TATACAATAA CAAAGCTGCA 960

GTATTGAAGT ATGAGAACAA TGTCATGAAT ATCAGGCAAT TCAACTGTTC TCCTCATCCA 1020

TACTGGCTTC CAAATTTCAT GGATGTTTTC ACCTGGTCCC TGCCGTTTGT TGGGGAAAAA 1080

GTGACTGAGA TGCTGGTAAA TGTCCTCAAC ATATGCTCAG ACGATGAACT AGGGTCAGAA 1140

GAAGATGGAT TTGATGGAGC AACGGCTGCA GCCAGGAAGG AGGTGATAAG AAATAAGATC 1200

CGAGCCATTG GCAAGATGGC CAGAGTGTTC TCGGTTCTCA GAGAAGAGAG TGAGAGTGTG 1260

CTGACGCTGA AAGGCCTGAC GCCCACAGGC ATGCTCCCCA GCGGCGTGCT TTCTGGAGGG 1320

AAACAAACCC TGCAAAGCGC TATCAAAGGA TTTTCACCAC AACATAAGAT CACTAGCTTC 1380

GAGGAGGCCA AGGGCTTAGA CCGAATTAAT GAGAGGATGC CACCTCGCAG AGATGCCATG 1440

CCCTCTGATG CCAACCTTAA CTCCATCAAC AAGGCTCTCG CCTCAGAGAC TAACGGCACG 1500

GACAGCAATG GCAGTAATAG CAGCAATATC CAGTGA 1536

Domain Profile

S: 1     pvtvcgdihgqffdllklfevggspantrylflgdyvdrgyfsiecvlylyalkilypkt  60

         pvtvcgdihgqffdl+klfevggspantrylflgdyvdrgyfsiecvlyl+alkilypkt

Q: 84    PVTVCGDIHGQFFDLMKLFEVGGSPANTRYLFLGDYVDRGYFSIECVLYLWALKILYPKT  143

         9***********************************************************

S: 61    lfllrgnhecrhlteyftfkqelkikyservydacleafdalplaallnqqflcvhggls  120

         lfllrgnhecrhlteyftfkqe+kikyservydac++afd+lplaal+nqqflcvhggls

Q: 144   LFLLRGNHECRHLTEYFTFKQECKIKYSERVYDACMDAFDCLPLAALMNQQFLCVHGGLS  203

         ************************************************************

S: 121   pelktlddirkldrfkeppayglmcdllwsdpledfgnekseeefvhnsvrgcsyfysyk  180

         pe++tlddirkldrfkeppayg+mcd+lwsdpledfgnek++e+f+hn+vrgcsyfysy+

Q: 204   PEINTLDDIRKLDRFKEPPAYGPMCDILWSDPLEDFGNEKTQEHFTHNTVRGCSYFYSYP  263

         ************************************************************

S: 181   avceflqennllsiiraheaq  201

         avceflq+nnllsi+raheaq

Q: 264   AVCEFLQHNNLLSILRAHEAQ  284

         ********************8

Domain Sequence
(FASTA)

PVTVCGDIHG QFFDLMKLFE VGGSPANTRY LFLGDYVDRG YFSIECVLYL WALKILYPKT 60

LFLLRGNHEC RHLTEYFTFK QECKIKYSER VYDACMDAFD CLPLAALMNQ QFLCVHGGLS 120

PEINTLDDIR KLDRFKEPPA YGPMCDILWS DPLEDFGNEK TQEHFTHNTV RGCSYFYSYP 180

AVCEFLQHNN LLSILRAHEA Q 201

Keyword

3D-structure; Alternative splicing; Calmodulin-binding; Complete proteome; Hydrolase; Iron; Metal-binding; Nucleus; Phosphoprotein; Protein phosphatase; Reference proteome; Zinc.

Sequence Source

Ensembl

Orthology

Ortholog group

Ailuropoda melanoleuca"; ?>Anolis carolinensis"; ?>Caenorhabditis elegans"; ?>Callithrix jacchus"; ?>Canis familiaris"; ?>Cavia porcellus"; ?>Choloepus hoffmanni"; ?>Ciona intestinalis"; ?>Ciona savignyi"; ?>Danio rerio"; ?>Drosophila melanogaster"; ?>Equus caballus"; ?>Felis catus"; ?>Gadus morhua"; ?>Gallus gallus"; ?>Gasterosteus aculeatus"; ?>Gorilla gorilla"; ?>Homo sapiens"; ?>Ictidomys tridecemlineatus"; ?>Loxodonta africana"; ?>Macaca mulatta"; ?>Meleagris gallopavo"; ?>Monodelphis domestica"; ?>Mus musculus"; ?>Mustela putorius furo"; ?>Myotis lucifugus"; ?>Nomascus leucogenys"; ?>Oreochromis niloticus"; ?>Ornithorhynchus anatinus"; ?>Oryctolagus cuniculus"; ?>Oryzias latipes"; ?>Otolemur garnettii"; ?>Pan troglodytes"; ?>Pelodiscus sinensis"; ?>Petromyzon marinus"; ?>Pongo abelii"; ?>Procavia capensis"; ?>Pteropus vampyrus"; ?>Rattus norvegicus"; ?>Sarcophilus harrisii"; ?>Sus scrofa"; ?>Taeniopygia guttata"; ?>Takifugu rubripes"; ?>Tarsius syrichta"; ?>Tetraodon nigroviridis"; ?>Tursiops truncatus"; ?>Vicugna pacos"; ?>Xenopus tropicalis"; ?>Xiphophorus maculatus"; ?>Saccharomyces cerevisiae"; ?>Schizosaccharomyces pombe"; ?>

Gene Ontology

GO:0005955	; C:calcineurin complex
GO:0005829	; C:cytosol
GO:0005739	; C:mitochondrion
GO:0005634	; C:nucleus
GO:0030018	; C:Z disc
GO:0033192	; F:calmodulin-dependent protein phosphatase activity
GO:0008144	; F:drug binding
GO:0046872	; F:metal ion binding
GO:0033173	; P:calcineurin-NFAT signaling cascade
GO:0006816	; P:calcium ion transport
GO:0035690	; P:cellular response to drug
GO:0000082	; P:G1/S transition of mitotic cell cycle
GO:0045944	; P:positive regulation of transcription from RNA polymerase II promoter
GO:0006470	; P:protein dephosphorylation
GO:0006606	; P:protein import into nucleus
GO:0060079	; P:regulation of excitatory postsynaptic membrane potential
GO:0050804	; P:regulation of synaptic transmission
GO:0051592	; P:response to calcium ion
GO:0006950	; P:response to stress
GO:0048741	; P:skeletal muscle fiber development
GO:0014883	; P:transition between fast and slow fiber

KEGG

bta:286852

;

InterPros

IPR004843	; Metallo_PEstase_dom.
IPR006186	; Ser/Thr-sp_prot-phosphatase.

Pfam

PF00149

; Metallophos; 1.

SMARTs

SM00156

; PP2Ac; 1.

Prosites

PS00125

; SER_THR_PHOSPHATASE; 1.

Prints

PR00114

; STPHPHTASE.

Created Date

20-Feb-2013