| Tag | Content |
|---|
| EKPD ID | EKS-BOT-00232 |
| Classification | | Group/Family | Score | E-Value | Start | End | Domain Length |
|---|
| CAMK/MAPKAPK | 426.5 | 3.9E-128 | 46 | 306 | 261 |
|
| Status | Reviewed |
| Ensembl Protein | ENSBTAP00000021983 |
| UniProt Accession | Q3SYZ2; Q0V8M1; |
| Protein Name | MAP kinase-activated protein kinase 3 |
| Protein Synonyms/Alias | MAPK-activated protein kinase 3; MAPKAP kinase 3; MAPKAP-K3; MAPKAPK-3; MK-3; |
| Gene Name | MAPKAPK3 |
| Gene Synonyms/Alias | MAPKAPK3; |
| Ensembl Information | |
| Organism | Bos taurus |
| Functional Description | Stress-activated serine/threonine-protein kinaseinvolved in cytokines production, endocytosis, cell migration, chromatin remodeling and transcriptional regulation. Following stress, it is phosphorylated and activated by MAP kinase p38- alpha/MAPK14, leading to phosphorylation of substrates. Phosphorylates serine in the peptide sequence, Hyd-X-R-X(2)-S, where Hyd is a large hydrophobic residue. MAPKAPK2 and MAPKAPK3, share the same function and substrate specificity, but MAPKAPK3 kinase activity and level in protein expression are lower compared to MAPKAPK2. Phosphorylates HSP27/HSPB1, KRT18, KRT20, RCSD1, RPS6KA3, TAB3 and TTP/ZFP36. Mediates phosphorylation of HSP27/HSPB1 in response to stress, leading to dissociate HSP27/HSPB1 from large small heat-shock protein (sHsps) oligomers and impair their chaperone activities and ability to protect against oxidative stress effectively. Involved in inflammatory response by regulating tumor necrosis factor (TNF) and IL6 production post-transcriptionally: acts by phosphorylating AU-rich elements (AREs)-binding proteins, such as TTP/ZFP36, leading to regulate the stability and translation of TNF and IL6 mRNAs. Phosphorylation of TTP/ZFP36, a major post-transcriptional regulator of TNF, promotes its binding to 14-3-3 proteins and reduces its ARE mRNA affinity leading to inhibition of dependent degradation of ARE-containing transcript. Involved in toll-like receptor signaling pathway (TLR) in dendritic cells: required for acute TLR-induced macropinocytosis by phosphorylating and activating RPS6KA3. Also acts as a modulator of Polycomb-mediated repression (By similarity). |
| Protein Length | 384 |
Protein Sequence
(FASTA) | | MDVETAEEQG GPAPPSGVPC GPCSAGAPAL GGRREPKKYA VTDDYQLSKQ VLGLGVNGKV 60 | | LECFHRRTGQ KCALKLLYDS PKARQEVDHH WQASGGPHIV RILDVYENMH HSKRCLLIIM 120 | | ECMEGGELFS RIQERGDQAF TEREAAEIMR DIGTAIQFLH SRNIAHRDVK PENLLYTSKD 180 | | KDAVLKLTDF GFAKETTQNA LQTPCYTPYY VAPEVLGPEK YDKSCDMWSL GVIMYILLCG 240 | | FPPFYSNTGQ AISPGMKRRI RLGQYGFPSP EWSEVSEDAK QLIRLLLKTD PTERLTITQF 300 | | MNHPWINQSM VVPQTPLHTA RVLQEDRDHW DEVKEEMTSA LATMRVDYDQ VKIKDLKTSN 360 | | NRLLNKRRKK QAGSSSGSQG CNNQ 384 |
|
Nucleotide Sequence
(FASTA) | | ATGGACGTGG AGACAGCAGA GGAGCAGGGG GGCCCTGCGC CCCCGTCAGG TGTACCCTGC 60 | | GGACCTTGTT CCGCCGGCGC TCCCGCCCTC GGGGGGCGGC GGGAGCCCAA GAAGTACGCA 120 | | GTGACCGATG ACTACCAGTT GTCCAAGCAG GTGCTGGGCT TGGGTGTGAA CGGCAAGGTG 180 | | CTGGAGTGCT TCCACCGGCG CACTGGGCAG AAGTGTGCCC TGAAGCTCCT GTACGACAGC 240 | | CCCAAGGCCC GGCAGGAGGT GGACCACCAC TGGCAGGCCT CCGGTGGCCC CCACATCGTG 300 | | CGCATCCTGG ATGTTTATGA GAATATGCAT CACAGCAAAC GCTGTCTCCT CATCATCATG 360 | | GAATGCATGG AAGGTGGTGA GTTGTTCAGC AGGATTCAGG AGCGTGGTGA CCAGGCTTTC 420 | | ACTGAAAGAG AAGCCGCAGA GATCATGCGG GATATTGGCA CTGCCATCCA GTTTCTGCAC 480 | | AGCCGGAACA TCGCCCACCG AGATGTCAAG CCTGAAAACC TGCTCTACAC GTCCAAGGAT 540 | | AAAGATGCGG TGCTGAAGCT CACGGACTTT GGCTTTGCCA AAGAGACCAC CCAGAATGCC 600 | | CTGCAAACTC CATGTTACAC TCCCTACTAT GTGGCTCCTG AGGTTCTGGG TCCAGAGAAG 660 | | TATGACAAGT CATGTGACAT GTGGTCCCTG GGCGTCATCA TGTACATCCT CCTGTGCGGC 720 | | TTCCCGCCCT TCTACTCCAA CACGGGCCAG GCCATCTCCC CGGGGATGAA GAGGAGGATC 780 | | CGCCTGGGCC AGTATGGCTT CCCCAGCCCT GAGTGGTCCG AGGTCTCCGA GGATGCCAAG 840 | | CAGCTGATCC GCCTCCTGCT GAAGACGGAC CCCACCGAGA GGCTGACCAT CACGCAATTC 900 | | ATGAACCATC CCTGGATCAA CCAATCGATG GTGGTGCCCC AGACTCCACT CCACACGGCC 960 | | CGAGTGCTGC AGGAGGACAG AGACCACTGG GATGAAGTCA AGGAGGAGAT GACCAGCGCC 1020 | | TTGGCAACCA TGCGGGTGGA CTATGACCAG GTGAAGATCA AGGACCTGAA GACCTCTAAC 1080 | | AACCGGCTCC TCAACAAGCG GAGGAAGAAG CAGGCAGGCA GCTCCTCCGG CTCGCAGGGC 1140 | | TGCAACAATC AGTAG 1155 |
|
| Domain Profile | S: 1 klteevlGeGisgkvlecvkkktgekyalkilkdrkkvrrevelhvqasghknivelidv 60 | +l+++vlG G++gkvlec++++tg+k+alk+l+d++k+r+ev++h+qasg ++iv+++dv | Q: 46 QLSKQVLGLGVNGKVLECFHRRTGQKCALKLLYDSPKARQEVDHHWQASGGPHIVRILDV 105 | 5789******************************************************** |
| S: 61 fensfedekrlllvlekleGGellsriqer..kaftekeasevvkdiasalkflhsknia 118 | +en+++ +++ll+++e++eGGel+sriqer +afte+ea+e+++di++a++flhs+nia | Q: 106 YENMHHSKRCLLIIMECMEGGELFSRIQERgdQAFTEREAAEIMRDIGTAIQFLHSRNIA 165 | *******************************9**************************** |
| S: 119 hrDlkpenlLyeskeknapvklcDfglaketeeeeelktpcltaeyvaPevleaekeeal 178 | hrD+kpenlLy+sk+k+a++kl+Dfg+ake +++++l+tpc+t++yvaPevl++ek | Q: 166 HRDVKPENLLYTSKDKDAVLKLTDFGFAKE-TTQNALQTPCYTPYYVAPEVLGPEK---- 220 | *****************************7.7899*********************.... |
| S: 179 lydksCDlWslGvilyillcGypPfyskkgraiskklkeriqegkyefPeeeWsevseea 238 | ydksCD+WslGvi+yillcG+pPfys++g+ais+++k+ri+ g+y fP++eWsevse+a | Q: 221 -YDKSCDMWSLGVIMYILLCGFPPFYSNTGQAISPGMKRRIRLGQYGFPSPEWSEVSEDA 279 | .*********************************************************** |
| S: 239 kdlirklLkrdpkerltieevlnhpwv 265 | k+lir lLk+dp+erlti++++nhpw+ | Q: 280 KQLIRLLLKTDPTERLTITQFMNHPWI 306 | **************************8 |
|
Domain Sequence
(FASTA) | | QLSKQVLGLG VNGKVLECFH RRTGQKCALK LLYDSPKARQ EVDHHWQASG GPHIVRILDV 60 | | YENMHHSKRC LLIIMECMEG GELFSRIQER GDQAFTEREA AEIMRDIGTA IQFLHSRNIA 120 | | HRDVKPENLL YTSKDKDAVL KLTDFGFAKE TTQNALQTPC YTPYYVAPEV LGPEKYDKSC 180 | | DMWSLGVIMY ILLCGFPPFY SNTGQAISPG MKRRIRLGQY GFPSPEWSEV SEDAKQLIRL 240 | | LLKTDPTERL TITQFMNHPW I 261 |
|
| Keyword | Acetylation; ATP-binding; Complete proteome; Cytoplasm; Kinase; Nucleotide-binding; Nucleus; Phosphoprotein; Reference proteome; Serine/threonine-protein kinase; Transferase. |
| Sequence Source | Ensembl |
| Orthology | |
| Gene Ontology | |
| KEGG | |
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| Pfam | |
| SMARTs | |
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| Prints | |
| Created Date | 20-Feb-2013 |