EPS-BOT-00018

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EPS-BOT-00018

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
PPM/PP2C	292.0	5.4E-87	23	289	267

Status

Reviewed

Ensembl Protein

ENSBTAP00000024128

UniProt Accession

O62829;

Protein Name

Protein phosphatase 1A

Protein Synonyms/Alias

Protein phosphatase 2C isoform alpha; PP2C-alpha;

Gene Name

PPM1A

Gene Synonyms/Alias

PPM1A;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
ENSBTAG00000018127	ENSBTAP00000024128	ENSBTAT00000024128

Organism

Bos taurus

Functional Description

Enzyme with a broad specificity. Negatively regulatesTGF-beta signaling through dephosphorylating SMAD2 and SMAD3, resulting in their dissociation from SMAD4, nuclear export of the SMADs and termination of the TGF-beta-mediated signaling (By similarity).

Protein Length

382

Protein Sequence
(FASTA)

MGAFLDKPKM EKHNAQGQGN GLRYGLSSMQ GWRVEMEDAH TAVIGLPSGL ETWSFFAVYD 60

GHAGSQVAKY CCEHLLDHIT NNQDFKGSAG APSVENVKNG IRTGFLEIDE HMRVMSEKKH 120

GADRSGSTAV GVLISPQHTY FINCGDSRGL LCRNRKVYFF TQDHKPSNPL EKERIQNAGG 180

SVMIQRVNGS LAVSRALGDF DYKCVHGKGP TEQLVSPEPE VHDIERSEED DQFIILACDG 240

IWDVMGNEEL CDFVRSRLEV TDDLEKVCNE VVDTCLYKGS RDNMSVILIC FPNAPKVSPE 300

AVKKEEELDK YLESRVEEII KKQGEGVPDL VHVMRTLASE NIPSLPPGGE LASKRNVIEA 360

VYNRLNPYKN DDTDSTSTDD MW 382

Nucleotide Sequence
(FASTA)

ATGGGAGCAT TTTTAGACAA ACCAAAGATG GAAAAACATA ATGCCCAGGG GCAGGGTAAT 60

GGGTTGCGAT ATGGGCTAAG CAGCATGCAA GGCTGGCGAG TTGAAATGGA GGATGCACAT 120

ACTGCTGTGA TCGGTCTGCC AAGTGGACTT GAAACATGGT CATTCTTTGC TGTGTATGAT 180

GGGCATGCTG GTTCTCAGGT TGCCAAATAC TGCTGTGAGC ATTTGTTAGA TCACATCACC 240

AATAACCAGG ATTTTAAAGG GTCTGCAGGA GCACCTTCTG TGGAAAATGT AAAGAATGGA 300

ATCAGAACAG GCTTCCTGGA GATTGATGAA CATATGAGAG TTATGTCAGA GAAGAAACAT 360

GGTGCTGATA GGAGTGGGTC AACAGCTGTG GGTGTCTTAA TTTCTCCTCA ACACACGTAT 420

TTCATTAACT GTGGAGACTC AAGAGGTTTA CTTTGTAGGA ACAGGAAAGT TTACTTCTTC 480

ACACAAGATC ACAAACCAAG TAATCCGCTG GAAAAAGAAC GAATTCAGAA TGCAGGTGGT 540

TCTGTAATGA TTCAGCGTGT GAATGGCTCT CTGGCTGTGT CGAGGGCCCT TGGGGACTTT 600

GATTACAAAT GTGTCCATGG AAAAGGTCCT ACAGAGCAGC TTGTTTCACC AGAGCCTGAA 660

GTCCATGATA TTGAAAGATC TGAAGAAGAT GATCAATTCA TTATTCTTGC ATGTGATGGT 720

ATTTGGGATG TCATGGGAAA TGAAGAGCTC TGTGATTTTG TAAGATCCAG ACTTGAAGTC 780

ACTGATGACC TTGAGAAAGT TTGCAATGAA GTAGTCGACA CCTGTTTGTA TAAGGGAAGT 840

CGAGACAACA TGAGTGTGAT ATTGATCTGT TTTCCAAATG CACCCAAAGT ATCGCCAGAA 900

GCAGTGAAGA AGGAGGAAGA GTTGGACAAA TACCTGGAAA GCAGAGTAGA AGAAATCATA 960

AAGAAGCAGG GGGAAGGCGT CCCTGACTTA GTCCATGTGA TGCGCACATT AGCGAGTGAG 1020

AACATACCCA GCCTCCCACC AGGGGGTGAA CTGGCAAGCA AGCGGAATGT AATTGAAGCC 1080

GTTTACAATA GACTGAATCC ATACAAAAAT GATGACACCG ACTCTACATC AACTGATGAT 1140

ATGTGGTAA 1149

Domain Profile

S: 2     lvgvvsvngrrnemedavtsspnflkspekkdrlaffgvyDGhggsevakfcseklheil  61

         ++g++s++g+r emeda+t++ ++ +     + ++ff+vyDGh+gs+vak+c e+l +++

Q: 23    RYGLSSMQGWRVEMEDAHTAVIGLPSGL---ETWSFFAVYDGHAGSQVAKYCCEHLLDHI  79

         69****************9855554444...45***************************

S: 62    aeele....sseseerkvkkalakaFlstdeelkvleeksldidksGstavvalikdskl  117

         +++ +    +++++ ++vk+ ++++Fl++de+++v++ek++  d+sGstav +li++++

Q: 80    TNNQDfkgsAGAPSVENVKNGIRTGFLEIDEHMRVMSEKKHGADRSGSTAVGVLISPQHT  139

         ***9999999999***********************************************

S: 118   yvanvGdSravLcrngkavelssdHkpsredEleRieeaggsvilkdgaRvlGvLavsRa  177

         y++n+GdSr +Lcrn+k+   ++dHkps++ E+eRi++aggsv+ +   Rv+G+LavsRa

Q: 140   YFINCGDSRGLLCRNRKVYFFTQDHKPSNPLEKERIQNAGGSVMIQ---RVNGSLAVSRA  196

         **********************************************...***********

S: 178   lGdlylk.........pyvtpePevtshrltsede.fliLasDGlwdvvsneeavslvrd  227

         lGd+++k         ++v+pePev+ +++++ed+ f+iLa+DG+wdv+ nee++++vr+

Q: 197   LGDFDYKcvhgkgpteQLVSPEPEVHDIERSEEDDqFIILACDGIWDVMGNEELCDFVRS  256

         ******************************999999************************

S: 228   vlekkeaeqeaakkLvklalargskdnvsaivv  260

         +le +++++++++++v+++l +gs+dn+s+i++

Q: 257   RLEVTDDLEKVCNEVVDTCLYKGSRDNMSVILI  289

         ****************************99976

Domain Sequence
(FASTA)

RYGLSSMQGW RVEMEDAHTA VIGLPSGLET WSFFAVYDGH AGSQVAKYCC EHLLDHITNN 60

QDFKGSAGAP SVENVKNGIR TGFLEIDEHM RVMSEKKHGA DRSGSTAVGV LISPQHTYFI 120

NCGDSRGLLC RNRKVYFFTQ DHKPSNPLEK ERIQNAGGSV MIQRVNGSLA VSRALGDFDY 180

KCVHGKGPTE QLVSPEPEVH DIERSEEDDQ FIILACDGIW DVMGNEELCD FVRSRLEVTD 240

DLEKVCNEVV DTCLYKGSRD NMSVILI 267

Keyword

Complete proteome; Hydrolase; Lipoprotein; Magnesium; Manganese; Metal-binding; Myristate; Nucleus; Phosphoprotein; Protein phosphatase; Reference proteome.

Sequence Source

Ensembl

Orthology

Ortholog group

Ailuropoda melanoleuca"; ?>Anolis carolinensis"; ?>Callithrix jacchus"; ?>Canis familiaris"; ?>Cavia porcellus"; ?>Ciona intestinalis"; ?>Ciona savignyi"; ?>Danio rerio"; ?>Dipodomys ordii"; ?>Echinops telfairi"; ?>Equus caballus"; ?>Erinaceus europaeus"; ?>Felis catus"; ?>Gadus morhua"; ?>Gallus gallus"; ?>Gasterosteus aculeatus"; ?>Gorilla gorilla"; ?>Homo sapiens"; ?>Ictidomys tridecemlineatus"; ?>Latimeria chalumnae"; ?>Loxodonta africana"; ?>Macaca mulatta"; ?>Meleagris gallopavo"; ?>Monodelphis domestica"; ?>Mus musculus"; ?>Mustela putorius furo"; ?>Myotis lucifugus"; ?>Nomascus leucogenys"; ?>Oreochromis niloticus"; ?>Ornithorhynchus anatinus"; ?>Oryctolagus cuniculus"; ?>Oryzias latipes"; ?>Otolemur garnettii"; ?>Pan troglodytes"; ?>Pelodiscus sinensis"; ?>Pongo abelii"; ?>Pteropus vampyrus"; ?>Rattus norvegicus"; ?>Sarcophilus harrisii"; ?>Sorex araneus"; ?>Sus scrofa"; ?>Taeniopygia guttata"; ?>Takifugu rubripes"; ?>Tarsius syrichta"; ?>Tetraodon nigroviridis"; ?>Tursiops truncatus"; ?>Vicugna pacos"; ?>Xenopus tropicalis"; ?>Xiphophorus maculatus"; ?>Saccharomyces cerevisiae"; ?>Schizosaccharomyces pombe"; ?>

Gene Ontology

GO:0005634	; C:nucleus
GO:0000287	; F:magnesium ion binding
GO:0030145	; F:manganese ion binding
GO:0004721	; F:phosphoprotein phosphatase activity
GO:0004722	; F:protein serine/threonine phosphatase activity
GO:0070412	; F:R-SMAD binding
GO:0004871	; F:signal transducer activity
GO:0043123	; P:positive regulation of I-kappaB kinase/NF-kappaB cascade
GO:0006470	; P:protein dephosphorylation

KEGG

bta:281994

;

InterPros

IPR001932	; PP2C-like.
IPR012911	; PP2C_C.
IPR000222	; PP2C_Mn2_Asp60_BS.
IPR015655	; Protein_Pase_2C.

Pfam

PF00481	; PP2C; 1.
PF07830	; PP2C_C; 1.

SMARTs

SM00331	; PP2C_SIG; 1.
SM00332	; PP2Cc; 1.

Prosites

PS01032

; PP2C; 1.

Prints

Created Date

20-Feb-2013