EKS-BOT-00263

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EKS-BOT-00263

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
Other/PLK	389.4	8.5E-117	12	265	254

Status

Reviewed

Ensembl Protein

ENSBTAP00000048808

UniProt Accession

A2VDZ4;

Protein Name

Serine/threonine-protein kinase PLK4

Protein Synonyms/Alias

Polo-like kinase 4; PLK-4; Serine/threonine-protein kinase Sak;

Gene Name

PLK4

Gene Synonyms/Alias

PLK4; SAK;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
ENSBTAG00000039552	ENSBTAP00000048808	ENSBTAT00000055868

Organism

Bos taurus

Functional Description

Serine/threonine-protein kinase that plays a centralrole in centriole duplication. Able to trigger procentriole formation on the surface of the parental centriole cylinder, leading to the recruitment of centriole biogenesis proteins such as SASS6, CENPJ/CPAP, CCP110, CEP135 and gamma-tubulin. When overexpressed, it is able to induce centrosome amplification through the simultaneous generation of multiple procentrioles adjoining each parental centriole during S phase. Phosphorylates 'Ser-151' of FBXW5 during the G1/S transition, leading to inhibit FBXW5 ability to ubiquitinate SASS6. Its central role in centriole replication suggests a posible role in tumorigenesis, centrosome aberrations being frequently observed in tumors. Also involved in trophoblast differentiation by phosphorylating HAND1, leading to disrupt the interaction between HAND1 and MDFIC and activate HAND1. Phosphorylates CDC25C and CHEK2 (By similarity).

Protein Length

893

Protein Sequence
(FASTA)

MATCIGEKIE DFRVGNLLGK GSFAGVYRAE SIHTGLEVAI KMIDKKAMYK AGMVQRVQNE 60

VKIHCQLKHP SILELYNYFE DNNYVYLVLE MCHNGEMNRY LKNRRKPFSE NEARHFMHQI 120

ITGMLYLHSH GILHRDLTLS NLLLTRNMNI KIADFGLAAQ LKMPHEKHYT LCGTPNYISP 180

EIATRSAHGL ESDIWSLGCM FYTLLIGRPP FDTDTVKNTL NKVVLADYEM PTFLSREAKD 240

LIHQLLRRNP ADRLSLSSVL DHPFMSRNSS KKSKDLGTVE DSIDSGHATI STAITASSST 300

SISGSLFDRR RLLIEQPLPN KMTIFPKNKN PSDFSSSGDG ISFYTSWGNQ EQETSNSGRG 360

RVIQEAEERP HSRYLRRAHS SDRSETSHGQ SRVKTYTMER CYSAEMLSKS KRSGVEENER 420

YSPTNNDANI FHFFKEKTSN SSGSFEGPDN NQALSNHLCP GKTPFPFPEQ TPQTEMVQQW 480

FGNLQINDPS CEQSKTRGVE PPLVYQKRTL RSITSPLTAY RLKPIRQKTK KAVVSILDSE 540

EVCVELLKDY ASQEYVKEVL QISSDGSMIT IYYPNDGRGF LLADRPPSPT DNISRYSFDN 600

LPEKYWRKYQ YASRFVQLVR SKSPKITYFT RYAKCVLMEN SPGADFEVWF YDGAKIHKTE 660

DLIQVIEKTG RSYTLKGESE VNSLKEEVKM YMNHANEGHR ICLALESIIS EEEKKSGSAP 720

FFPIIVGRRP SSTSSPKALT PPPPVDPNYP MRETPSLNRM IINSAASPKQ APVLNPPVVT 780

NEGLGLMGAA SETNSSPRSL KDCLPKSAQL LKSVFVKNVG WATQLTSGAV WVQFNDGSQL 840

VVQAGVSSIS YTSPDGQTTR YGENEKLPEY IKQKLQCLSS ILLMFSNPTP SFH 893

Nucleotide Sequence
(FASTA)

ATGGCGACCT GCATCGGAGA AAAGATCGAG GATTTTAGAG TTGGAAATCT GCTTGGTAAA 60

GGATCATTTG CTGGTGTTTA TAGAGCTGAG TCCATTCACA CTGGTTTGGA AGTTGCAATC 120

AAAATGATAG ATAAGAAAGC CATGTACAAA GCTGGAATGG TACAGAGAGT CCAAAATGAG 180

GTGAAAATAC ATTGCCAATT GAAACATCCT TCTATCTTGG AGCTGTATAA CTATTTTGAA 240

GATAACAATT ATGTGTACCT AGTATTAGAA ATGTGCCATA ACGGAGAAAT GAACAGGTAT 300

TTAAAAAACA GAAGAAAACC ATTCTCAGAA AATGAAGCTC GACACTTCAT GCACCAAATC 360

ATCACAGGAA TGTTATATCT CCATTCACAT GGTATATTAC ACCGGGACCT CACACTTTCT 420

AACCTCTTAC TTACACGTAA TATGAACATC AAGATTGCTG ATTTTGGGCT GGCAGCTCAG 480

TTGAAAATGC CACATGAAAA GCACTATACA TTATGTGGAA CTCCTAATTA CATTTCTCCA 540

GAAATTGCAA CTCGAAGTGC ACATGGACTT GAATCTGATA TTTGGTCCTT GGGCTGTATG 600

TTTTATACGT TACTTATTGG GAGACCGCCT TTTGACACTG ACACAGTCAA GAACACATTA 660

AATAAAGTAG TATTGGCAGA TTATGAAATG CCAACTTTTT TGTCAAGAGA AGCCAAGGAC 720

CTTATTCACC AGTTACTTCG TAGAAATCCA GCAGATCGTT TAAGTCTGTC TTCAGTATTA 780

GATCATCCTT TTATGTCCCG AAATTCTTCA AAAAAAAGCA AGGATTTAGG AACTGTAGAA 840

GACTCGATTG ATAGTGGACA TGCCACAATT TCTACAGCAA TTACAGCTTC TTCCAGTACC 900

AGTATAAGTG GTAGTTTATT TGACAGAAGA CGACTTTTGA TTGAACAGCC ACTCCCAAAT 960

AAAATGACTA TATTTCCAAA GAATAAAAAT CCAAGTGATT TTTCTTCTTC AGGAGATGGA 1020

ATCAGCTTTT ATACTTCATG GGGCAATCAA GAACAAGAAA CCAGTAATAG TGGAAGGGGA 1080

AGAGTAATCC AAGAGGCAGA AGAAAGACCA CATTCTCGAT ACCTTCGTAG AGCCCATTCG 1140

TCTGATAGAT CTGAGACTTC TCATGGTCAG TCTAGAGTGA AAACATACAC AATGGAACGA 1200

TGTTACTCAG CAGAAATGCT TTCAAAATCC AAACGATCAG GAGTGGAGGA AAATGAAAGA 1260

TACTCACCCA CAAACAACGA TGCTAATATT TTTCACTTCT TTAAGGAAAA AACATCCAAT 1320

AGTTCTGGAT CTTTTGAAGG ACCTGATAAC AATCAAGCAC TCTCCAATCA TCTTTGTCCA 1380

GGAAAAACTC CCTTTCCATT TCCTGAACAG ACACCTCAGA CTGAAATGGT GCAACAATGG 1440

TTTGGGAATC TGCAAATAAA TGATCCTTCT TGTGAACAAA GCAAGACTAG GGGTGTGGAG 1500

CCACCGTTGG TCTATCAGAA GCGTACATTA CGAAGCATTA CATCTCCTTT GACTGCTTAT 1560

AGGTTAAAAC CAATCCGACA GAAAACCAAA AAGGCCGTGG TGAGCATACT TGATTCAGAG 1620

GAGGTATGTG TGGAGCTTCT GAAGGATTAT GCATCTCAAG AATATGTGAA AGAAGTTCTT 1680

CAGATATCTA GTGACGGGAG TATGATCACT ATTTATTATC CAAATGATGG AAGAGGTTTT 1740

CTTCTTGCTG ATAGACCACC CTCACCTACT GATAACATCA GTAGGTACAG TTTTGATAAT 1800

TTACCAGAAA AGTACTGGCG AAAATATCAA TATGCTTCCA GATTTGTACA GCTTGTAAGA 1860

TCTAAATCTC CTAAAATTAC TTATTTTACA AGATATGCTA AATGTGTTTT GATGGAGAAT 1920

TCCCCTGGTG CTGATTTTGA GGTTTGGTTT TATGATGGAG CAAAGATACA CAAAACAGAA 1980

GATTTAATTC AGGTGATCGA AAAGACTGGC AGATCTTACA CCTTAAAAGG TGAAAGTGAA 2040

GTTAATAGCT TAAAAGAGGA GGTAAAAATG TATATGAACC ATGCTAATGA GGGCCACCGT 2100

ATTTGTTTAG CACTGGAATC CATAATTTCA GAAGAGGAAA AGAAAAGTGG AAGTGCTCCC 2160

TTTTTCCCCA TAATCGTAGG AAGAAGACCT AGTAGTACTA GTTCACCTAA GGCCTTAACA 2220

CCTCCTCCTC CTGTGGATCC AAACTACCCA ATGAGAGAGA CACCATCTCT GAATAGAATG 2280

ATCATAAATA GTGCTGCTTC TCCAAAACAG GCACCAGTAC TTAATCCTCC TGTGGTTACA 2340

AATGAAGGAC TTGGCCTTAT GGGTGCAGCT TCTGAAACAA ACAGCTCTCC TAGAAGTCTT 2400

AAAGATTGTC TCCCTAAATC AGCACAACTT TTGAAGTCTG TTTTTGTGAA AAATGTTGGT 2460

TGGGCTACAC AGTTAACTAG TGGAGCTGTG TGGGTTCAGT TTAATGATGG GTCCCAGTTG 2520

GTCGTGCAGG CAGGAGTGTC TTCTATTAGT TATACATCAC CAGATGGTCA AACAACTAGG 2580

TATGGAGAAA ATGAAAAATT ACCAGAATAC ATCAAACAGA AATTACAGTG TCTTTCTTCC 2640

ATCCTTTTGA TGTTTTCTAA TCCAACTCCT AGTTTTCATT GA 2682

Domain Profile

S: 1     yergkllGkGgfakcyelkdletkevfavkvvpksllakekqrekvekeieihrslkhkn  60

         +++g+llGkG+fa +y++++++t+ ++a+k+++k++++k+ + ++v++e++ih++lkh++

Q: 12    FRVGNLLGKGSFAGVYRAESIHTGLEVAIKMIDKKAMYKAGMVQRVQNEVKIHCQLKHPS  71

         6789********************************************************

S: 61    ivklyeffedkenvylvlelcsrrslaellkrr.kaltepevryylrqivsglkylhekr  119

         i++ly++fed+++vylvle+c++++++++lk+r k+++e+e+r++++qi++g++ylh++

Q: 72    ILELYNYFEDNNYVYLVLEMCHNGEMNRYLKNRrKPFSENEARHFMHQIITGMLYLHSHG  131

         ******************************98879*************************

S: 120   ilhrdlklgnlflneelevkigdfGlatrlekeeerkktlcGtPnyiaPevlskkghsle  179

         ilhrdl+l+nl+l++++++ki+dfGla++l++++e+++tlcGtPnyi+Pe++++++h+le

Q: 132   ILHRDLTLSNLLLTRNMNIKIADFGLAAQLKMPHEKHYTLCGTPNYISPEIATRSAHGLE  191

         ************************************************************

S: 180   vdiWslGcilytllvGkPPfetkelketyekikkaeyslPsslskeaksliakllkkePe  239

         +diWslGc++ytll+G+PPf+t+++k+t++k++ a+y++P+ ls+eak+li++ll+++P+

Q: 192   SDIWSLGCMFYTLLIGRPPFDTDTVKNTLNKVVLADYEMPTFLSREAKDLIHQLLRRNPA  251

         ************************************************************

S: 240   erpsleevlkdefl  253

         +r+sl++vl+++f+

Q: 252   DRLSLSSVLDHPFM  265

         ************98

Domain Sequence
(FASTA)

FRVGNLLGKG SFAGVYRAES IHTGLEVAIK MIDKKAMYKA GMVQRVQNEV KIHCQLKHPS 60

ILELYNYFED NNYVYLVLEM CHNGEMNRYL KNRRKPFSEN EARHFMHQII TGMLYLHSHG 120

ILHRDLTLSN LLLTRNMNIK IADFGLAAQL KMPHEKHYTL CGTPNYISPE IATRSAHGLE 180

SDIWSLGCMF YTLLIGRPPF DTDTVKNTLN KVVLADYEMP TFLSREAKDL IHQLLRRNPA 240

DRLSLSSVLD HPFM 254

Keyword

ATP-binding; Complete proteome; Cytoplasm; Cytoskeleton; Kinase; Nucleotide-binding; Nucleus; Phosphoprotein; Reference proteome; Serine/threonine-protein kinase; Transferase; Ubl conjugation.

Sequence Source

Ensembl

Orthology

Ortholog group

Ailuropoda melanoleuca"; ?>Anolis carolinensis"; ?>Callithrix jacchus"; ?>Canis familiaris"; ?>Cavia porcellus"; ?>Ciona intestinalis"; ?>Ciona savignyi"; ?>Danio rerio"; ?>Dipodomys ordii"; ?>Drosophila melanogaster"; ?>Equus caballus"; ?>Felis catus"; ?>Gallus gallus"; ?>Gasterosteus aculeatus"; ?>Gorilla gorilla"; ?>Homo sapiens"; ?>Ictidomys tridecemlineatus"; ?>Latimeria chalumnae"; ?>Loxodonta africana"; ?>Macaca mulatta"; ?>Meleagris gallopavo"; ?>Monodelphis domestica"; ?>Mus musculus"; ?>Mustela putorius furo"; ?>Myotis lucifugus"; ?>Nomascus leucogenys"; ?>Oreochromis niloticus"; ?>Ornithorhynchus anatinus"; ?>Oryctolagus cuniculus"; ?>Oryzias latipes"; ?>Otolemur garnettii"; ?>Pelodiscus sinensis"; ?>Petromyzon marinus"; ?>Pongo abelii"; ?>Rattus norvegicus"; ?>Sarcophilus harrisii"; ?>Sus scrofa"; ?>Taeniopygia guttata"; ?>Takifugu rubripes"; ?>Tetraodon nigroviridis"; ?>Vicugna pacos"; ?>Xenopus tropicalis"; ?>Xiphophorus maculatus"; ?>

Gene Ontology

GO:0005814	; C:centriole
GO:0005730	; C:nucleolus
GO:0005524	; F:ATP binding
GO:0004674	; F:protein serine/threonine kinase activity
GO:0004713	; F:protein tyrosine kinase activity
GO:0007099	; P:centriole replication
GO:0046601	; P:positive regulation of centriole replication
GO:0060707	; P:trophoblast giant cell differentiation

KEGG

bta:514405

;

InterPros

IPR011009	; Kinase-like_dom.
IPR000959	; POLO_box_duplicated_dom.
IPR000719	; Prot_kinase_cat_dom.
IPR017441	; Protein_kinase_ATP_BS.
IPR002290	; Ser/Thr_dual-sp_kinase_dom.
IPR008266	; Tyr_kinase_AS.

Pfam

PF00069	; Pkinase; 1.
PF00659	; POLO_box; 1.

SMARTs

SM00220

; S_TKc; 1.

Prosites

PS50078	; POLO_BOX; 1.
PS00107	; PROTEIN_KINASE_ATP; 1.
PS50011	; PROTEIN_KINASE_DOM; 1.
PS00108	; PROTEIN_KINASE_ST; FALSE_NEG.

Prints

Created Date

20-Feb-2013