EKS-CAF-00402

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EKS-CAF-00402

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
TK/PDGFR	601.8	5.3E-181	576	911	336

Status

Reviewed

Ensembl Protein

ENSCAFP00000003042

UniProt Accession

O97799; Q7YRV7; Q8WN23;

Protein Name

Mast/stem cell growth factor receptor Kit

Protein Synonyms/Alias

SCFR; Proto-oncogene c-Kit; Tyrosine-protein kinase Kit;

Gene Name

KIT

Gene Synonyms/Alias

KIT;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
ENSCAFG00000002065	ENSCAFP00000003042	ENSCAFT00000003274
ENSCAFG00000002065	ENSCAFP00000039467	ENSCAFT00000049830

Organism

Canis familiaris

Functional Description

Tyrosine-protein kinase that acts as cell-surfacereceptor for the cytokine KITLG/SCF and plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. In response to KITLG/SCF binding, KIT can activate several signaling pathways. Phosphorylates PIK3R1, PLCG1, SH2B2/APS and CBL. Activates the AKT1 signaling pathway by phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase. Activated KIT also transmits signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. Promotes activation of STAT family members STAT1, STAT3, STAT5A and STAT5B. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. KIT signaling is modulated by protein phosphatases, and by rapid internalization and degradation of the receptor. Activated KIT promotes phosphorylation of the protein phosphatases PTPN6/SHP-1 and PTPRU, and of the transcription factors STAT1, STAT3, STAT5A and STAT5B. Promotes phosphorylation of PIK3R1, CBL, CRK (isoform Crk-II), LYN, MAPK1/ERK2 and/or MAPK3/ERK1, PLCG1, SRC and SHC1 (By similarity).

Protein Length

963

Protein Sequence
(FASTA)

VLFLLFLSRV SACSSQPSVS PGEPSLPSIH PAKSELIVSV GDELRLSCTD PGFVKWTFET 60

LGQLNENTHN EWITEKAEAG HTGNYTCTNR DGLSRSIYVF VRDPAKLFLV DLPLYGKEGN 120

DTLVRCPLTD PEVTNYSLRG CEGKPLPKDL TFVADPKAGI TIRNVKREYH RLCLHCSADQ 180

KGRTVLSKKF TLKVRAAIRA VPVVSVSKTS SLLKEGEAFS VMCFIKDVSS FVDSMWIKEN 240

SQQTNAQTQS NSWHHGDFNF ERQEKLIISS ARVNDSGVFM CYANNTFGSA NVTTTLEVVD 300

KGFINIFPMM STTIFVNDGE NVDLIVEYEA YPKPEHQQWI YMNRTFTDKW EDYPKSDNES 360

NIRYVSELHL TRLKGNEGGT YTFQVSNSDV NSSVTFNVYV NTKPEILTHE SLTNGMLQCV 420

VAGFPEPAVD WYFCPGAEQR CSVPIGPMDV QMQNSSLSPS GKLVVQSSID YSAFKHNGTV 480

ECRAYNNVGR SSAFFNFAFK EQIHPHTLFT PLLIGFVIAA GMMCIIVMIL TYKYLQKPMY 540

EVQWKVVEEI NGNNYVYIDP TQLPYDHKWE FPRNRLSFGK TLGAGAFGKV VEATAYGLIK 600

SDAAMTVAVK MLKPSAHLTE REALMSELKV LSYLGNHMNI VNLLGACTVG GPTLVITEYC 660

CYGDLLNFLR RKRDSFICSK QEDHGEVALY KNLLHSKESS CSDSTNEYMD MKPGVSYVVP 720

TKADKRRSAR IGSYIERDVT PAIMEDDELA LDLEDLLSFS YQVAKGMAFL ASKNCIHRDL 780

AARNILLTHG RITKICDFGL ARDIKNDSNY VVKGNARLPV KWMAPESIFN CVYTFESDVW 840

SYGIFLWELF SLGSSPYPGM PVDSKFYKMI KEGFRMLSPE HAPAEMYDIM KTCWDADPLK 900

RPTFKQIVQL IEKQISDSTN HIYSNLANCS PNPERPVVDH SVRINSVGSS ASSTQPLLVH 960

EDV 963

Nucleotide Sequence
(FASTA)

GTTTTGTTTT TGTTGTTTCT AAGCAGGGTG TCAGCATGCT CTTCTCAACC ATCTGTGAGT 60

CCAGGGGAAC CGTCTCTCCC ATCCATCCAT CCAGCAAAAT CAGAGTTAAT AGTCAGTGTC 120

GGCGACGAGC TTAGGCTGTC CTGCACCGAC CCAGGATTTG TCAAGTGGAC TTTTGAGACC 180

CTGGGTCAAC TGAATGAGAA CACACACAAC GAATGGATCA CAGAGAAGGC AGAGGCTGGC 240

CACACGGGCA ATTACACGTG CACCAACAGA GATGGCTTGA GCAGGTCCAT TTATGTGTTT 300

GTCAGAGATC CTGCAAAGCT TTTCCTCGTT GACCTTCCCT TGTATGGGAA AGAAGGCAAT 360

GATACGCTGG TCCGCTGCCC TCTGACGGAC CCAGAAGTGA CCAATTACTC CCTCAGGGGG 420

TGCGAGGGGA AGCCTCTTCC CAAGGACTTG ACGTTCGTCG CTGATCCCAA AGCTGGCATC 480

ACGATCAGAA ACGTGAAGCG CGAGTATCAT CGGCTCTGCT TGCACTGCTC TGCGGACCAG 540

AAGGGCAGGA CGGTGCTGTC CAAGAAATTC ACCCTGAAAG TGAGGGCAGC CATCAGAGCT 600

GTACCAGTTG TGTCAGTATC CAAAACAAGC TCTCTCCTGA AGGAAGGGGA AGCCTTCTCT 660

GTGATGTGCT TTATAAAAGA TGTGTCTAGT TTCGTGGACT CGATGTGGAT AAAGGAAAAC 720

AGCCAGCAGA CTAATGCACA GACACAGAGT AATAGCTGGC ATCATGGTGA CTTCAATTTT 780

GAACGTCAGG AAAAGTTGAT TATCAGCTCA GCAAGAGTTA ATGATTCTGG AGTGTTCATG 840

TGTTACGCCA ATAATACTTT TGGATCAGCA AATGTCACAA CAACCTTGGA AGTAGTAGAT 900

AAAGGATTCA TTAATATCTT CCCCATGATG AGTACTACAA TATTTGTAAA TGATGGAGAG 960

AATGTGGATC TGATTGTTGA ATATGAGGCA TATCCCAAAC CGGAGCACCA GCAGTGGATC 1020

TATATGAACA GAACCTTCAC TGATAAATGG GAAGATTATC CCAAGTCTGA CAATGAAAGT 1080

AATATCAGAT ATGTGAGTGA ACTTCATCTA ACCAGATTAA AAGGGAACGA AGGAGGCACT 1140

TACACATTTC AAGTGTCCAA TTCCGATGTC AATTCTTCGG TGACATTTAA TGTTTATGTG 1200

AACACAAAAC CAGAAATCCT GACTCATGAA AGTCTCACAA ATGGCATGCT CCAGTGTGTG 1260

GTTGCAGGAT TCCCAGAGCC CGCAGTAGAT TGGTATTTCT GTCCAGGAGC TGAGCAGAGA 1320

TGTTCTGTCC CTATTGGGCC AATGGATGTG CAGATGCAAA ACTCGTCTCT GTCACCGTCT 1380

GGAAAACTAG TGGTTCAGAG TTCCATCGAT TATAGTGCCT TCAAGCACAA TGGCACAGTC 1440

GAGTGTAGGG CTTACAACAA TGTAGGCAGG AGTTCTGCCT TTTTTAACTT TGCATTTAAA 1500

GAACAAATCC ATCCCCACAC CCTGTTCACA CCTTTGCTGA TTGGCTTTGT GATCGCAGCT 1560

GGAATGATGT GCATTATCGT GATGATTCTT ACCTACAAGT ATCTACAGAA ACCCATGTAT 1620

GAAGTACAGT GGAAGGTTGT TGAGGAGATC AATGGAAACA ATTATGTTTA CATAGACCCA 1680

ACACAGCTTC CTTACGATCA CAAATGGGAG TTTCCCAGAA ACAGGCTGAG CTTTGGGAAA 1740

ACTTTGGGTG CTGGTGCCTT CGGGAAAGTG GTTGAAGCCA CTGCATATGG CCTGATTAAG 1800

TCGGATGCGG CCATGACTGT TGCCGTTAAG ATGCTCAAAC CAAGTGCCCA TTTAACCGAA 1860

CGAGAAGCCC TAATGTCTGA GCTCAAAGTC TTGAGTTACC TCGGTAATCA TATGAATATT 1920

GTGAATCTTC TTGGAGCGTG CACCGTTGGA GGGCCCACCT TGGTCATTAC TGAATATTGT 1980

TGCTATGGTG ATCTTTTGAA TTTTTTGCGA AGGAAACGTG ATTCATTTAT TTGCTCAAAG 2040

CAGGAAGATC ACGGAGAAGT GGCACTTTAT AAGAACCTTC TGCATTCAAA GGAGTCTTCC 2100

TGCAGTGACA GTACTAATGA ATACATGGAC ATGAAACCCG GCGTTTCTTA CGTTGTGCCA 2160

ACCAAGGCAG ACAAAAGGAG ATCTGCGAGA ATAGGCTCAT ACATAGAAAG GGATGTGACT 2220

CCTGCCATCA TGGAAGATGA TGAGTTGGCT CTAGATCTAG AGGACTTGCT GAGCTTTTCT 2280

TACCAGGTGG CCAAGGGTAT GGCATTCCTG GCCTCGAAGA ATTGTATTCA CAGAGACTTG 2340

GCTGCTAGAA ATATCCTCCT TACTCATGGT CGAATCACAA AGATTTGTGA TTTTGGTCTA 2400

GCCAGAGACA TCAAGAATGA TTCTAATTAT GTGGTCAAAG GAAACGCTCG GCTACCTGTG 2460

AAGTGGATGG CCCCTGAGAG CATTTTCAAC TGTGTGTACA CATTTGAAAG TGATGTCTGG 2520

TCCTATGGGA TTTTTCTGTG GGAGCTCTTC TCTTTAGGAA GCAGCCCCTA CCCTGGGATG 2580

CCAGTCGATT CAAAGTTCTA CAAGATGATC AAGGAAGGGT TCCGGATGCT CAGCCCTGAG 2640

CATGCACCTG CTGAAATGTA TGACATCATG AAGACGTGCT GGGATGCTGA TCCCCTGAAA 2700

AGGCCGACGT TCAAGCAGAT CGTGCAGCTA ATTGAGAAGC AGATTTCAGA TAGCACCAAT 2760

CATATTTATT CCAACCTCGC GAACTGCAGC CCCAACCCAG AGCGCCCCGT GGTGGACCAT 2820

TCCGTGCGGA TCAATTCCGT GGGCAGCAGC GCGTCTTCCA CCCAGCCTCT GCTGGTACAC 2880

GAAGATGTGT GA 2892

Domain Profile

S: 1     lelGkvlGsGafGkvveatayGlsksdavlkvavkmlkstarssekealmselkilshlG  60

         l++Gk+lG+GafGkvveatayGl+ksda+++vavkmlk++a+ +e+ealmselk+ls+lG

Q: 576   LSFGKTLGAGAFGKVVEATAYGLIKSDAAMTVAVKMLKPSAHLTEREALMSELKVLSYLG  635

         79**********************************************************

S: 61    ehenivnllGactkgGpiyviteyccyGdllnylrrkresflkkslekkkekeeeldska  120

         +h+nivnllGact+gGp++viteyccyGdlln+lrrkr+sf+   ++k++++ e + +k+

Q: 636   NHMNIVNLLGACTVGGPTLVITEYCCYGDLLNFLRRKRDSFI---CSKQEDHGEVALYKN  692

         ******************************************...999999999999999

S: 121   lladkslrsksslssesseeyvdlkkdvsdvssskadklasekkksalerevkaaleeed  180

         l     l+sk+s++s+s++ey+d+k++vs+v ++kadk++s+++ s++er+v++a++e+d

Q: 693   L-----LHSKESSCSDSTNEYMDMKPGVSYVVPTKADKRRSARIGSYIERDVTPAIMEDD  747

         9.....9*****************************************************

S: 181   sevleledllsfsyqvakGmeflaskncvhrdlaarnvllteGkvvkicdfGlardimkd  240

         + +l+ledllsfsyqvakGm+flasknc+hrdlaarn+llt+G+++kicdfGlardi++d

Q: 748   ELALDLEDLLSFSYQVAKGMAFLASKNCIHRDLAARNILLTHGRITKICDFGLARDIKND  807

         ************************************************************

S: 241   snyvvkGnarlpvkwmapesifdslytvksdvwsyGillweifslGstpypgllvdskfy  300

         snyvvkGnarlpvkwmapesif+++yt++sdvwsyGi+lwe+fslGs+pypg++vdskfy

Q: 808   SNYVVKGNARLPVKWMAPESIFNCVYTFESDVWSYGIFLWELFSLGSSPYPGMPVDSKFY  867

         ************************************************************

S: 301   klikeGyrmaqpayateeiyeimqkcweaepekrpsfkqlvell  344

         k+ikeG+rm +p++a++e+y+im++cw+a+p krp+fkq+v+l+

Q: 868   KMIKEGFRMLSPEHAPAEMYDIMKTCWDADPLKRPTFKQIVQLI  911

         *****************************************996

Domain Sequence
(FASTA)

LSFGKTLGAG AFGKVVEATA YGLIKSDAAM TVAVKMLKPS AHLTEREALM SELKVLSYLG 60

NHMNIVNLLG ACTVGGPTLV ITEYCCYGDL LNFLRRKRDS FICSKQEDHG EVALYKNLLH 120

SKESSCSDST NEYMDMKPGV SYVVPTKADK RRSARIGSYI ERDVTPAIME DDELALDLED 180

LLSFSYQVAK GMAFLASKNC IHRDLAARNI LLTHGRITKI CDFGLARDIK NDSNYVVKGN 240

ARLPVKWMAP ESIFNCVYTF ESDVWSYGIF LWELFSLGSS PYPGMPVDSK FYKMIKEGFR 300

MLSPEHAPAE MYDIMKTCWD ADPLKRPTFK QIVQLI 336

Keyword

Alternative splicing; ATP-binding; Cell membrane; Complete proteome; Disulfide bond; Glycoprotein; Immunoglobulin domain; Kinase; Magnesium; Membrane; Metal-binding; Nucleotide-binding; Phosphoprotein; Proto-oncogene; Receptor; Reference proteome; Repeat; Signal; Transferase; Transmembrane; Transmembrane helix; Tyrosine-protein kinase; Ubl conjugation.

Sequence Source

Ensembl

Orthology

Ortholog group

Ailuropoda melanoleuca"; ?>Anolis carolinensis"; ?>Bos taurus"; ?>Callithrix jacchus"; ?>Cavia porcellus"; ?>Danio rerio"; ?>Equus caballus"; ?>Felis catus"; ?>Gallus gallus"; ?>Gasterosteus aculeatus"; ?>Gorilla gorilla"; ?>Homo sapiens"; ?>Ictidomys tridecemlineatus"; ?>Latimeria chalumnae"; ?>Loxodonta africana"; ?>Macaca mulatta"; ?>Meleagris gallopavo"; ?>Monodelphis domestica"; ?>Mus musculus"; ?>Mustela putorius furo"; ?>Myotis lucifugus"; ?>Nomascus leucogenys"; ?>Oreochromis niloticus"; ?>Ornithorhynchus anatinus"; ?>Oryctolagus cuniculus"; ?>Oryzias latipes"; ?>Otolemur garnettii"; ?>Pan troglodytes"; ?>Pelodiscus sinensis"; ?>Pongo abelii"; ?>Pteropus vampyrus"; ?>Rattus norvegicus"; ?>Sarcophilus harrisii"; ?>Sus scrofa"; ?>Taeniopygia guttata"; ?>Takifugu rubripes"; ?>Tetraodon nigroviridis"; ?>Xenopus tropicalis"; ?>Xiphophorus maculatus"; ?>

Gene Ontology

GO:0005737	; C:cytoplasm
GO:0009897	; C:external side of plasma membrane
GO:0005615	; C:extracellular space
GO:0016021	; C:integral to membrane
GO:0005634	; C:nucleus
GO:0005524	; F:ATP binding
GO:0019955	; F:cytokine binding
GO:0046872	; F:metal ion binding
GO:0005020	; F:stem cell factor receptor activity
GO:0004714	; F:transmembrane receptor protein tyrosine kinase activity
GO:0031532	; P:actin cytoskeleton reorganization
GO:0000187	; P:activation of MAPK activity
GO:0060326	; P:cell chemotaxis
GO:0097067	; P:cellular response to thyroid hormone stimulus
GO:0002371	; P:dendritic cell cytokine production
GO:0050910	; P:detection of mechanical stimulus involved in sensory perception of sound
GO:0048565	; P:digestive tract development
GO:0035162	; P:embryonic hemopoiesis
GO:0030218	; P:erythrocyte differentiation
GO:0038162	; P:erythropoietin-mediated signaling pathway
GO:0038093	; P:Fc receptor signaling pathway
GO:0035234	; P:germ cell programmed cell death
GO:0006687	; P:glycosphingolipid metabolic process
GO:0002327	; P:immature B cell differentiation
GO:0006954	; P:inflammatory response
GO:0007243	; P:intracellular protein kinase cascade
GO:0038109	; P:Kit signaling pathway
GO:0030032	; P:lamellipodium assembly
GO:0002320	; P:lymphoid progenitor cell differentiation
GO:0008584	; P:male gonad development
GO:0002551	; P:mast cell chemotaxis
GO:0032762	; P:mast cell cytokine production
GO:0043303	; P:mast cell degranulation
GO:0060374	; P:mast cell differentiation
GO:0035855	; P:megakaryocyte development
GO:0097326	; P:melanocyte adhesion
GO:0030318	; P:melanocyte differentiation
GO:0097324	; P:melanocyte migration
GO:0002318	; P:myeloid progenitor cell differentiation
GO:0043069	; P:negative regulation of programmed cell death
GO:0001541	; P:ovarian follicle development
GO:0018108	; P:peptidyl-tyrosine phosphorylation
GO:0008284	; P:positive regulation of cell proliferation
GO:0010628	; P:positive regulation of gene expression
GO:0043410	; P:positive regulation of MAPK cascade
GO:0051091	; P:positive regulation of sequence-specific DNA binding transcription factor activity
GO:0042511	; P:positive regulation of tyrosine phosphorylation of Stat1 protein
GO:0042517	; P:positive regulation of tyrosine phosphorylation of Stat3 protein
GO:0042523	; P:positive regulation of tyrosine phosphorylation of Stat5 protein
GO:0046777	; P:protein autophosphorylation
GO:0008360	; P:regulation of cell shape
GO:0048070	; P:regulation of developmental pigmentation
GO:0009314	; P:response to radiation
GO:0007286	; P:spermatid development
GO:0007283	; P:spermatogenesis
GO:0048863	; P:stem cell differentiation
GO:0030217	; P:T cell differentiation

KEGG

cfa:403811

;

InterPros

IPR007110	; Ig-like.
IPR013783	; Ig-like_fold.
IPR013098	; Ig_I-set.
IPR003599	; Ig_sub.
IPR011009	; Kinase-like_dom.
IPR000719	; Prot_kinase_cat_dom.
IPR017441	; Protein_kinase_ATP_BS.
IPR001245	; Ser-Thr/Tyr_kinase_cat_dom.
IPR008266	; Tyr_kinase_AS.
IPR020635	; Tyr_kinase_cat_dom.
IPR016243	; Tyr_kinase_CSF1/PDGF_rcpt.
IPR001824	; Tyr_kinase_rcpt_3_CS.

Pfam

PF07679	; I-set; 1.
PF07714	; Pkinase_Tyr; 1.

SMARTs

SM00409	; IG; 3.
SM00219	; TyrKc; 1.

Prosites

PS50835	; IG_LIKE; 2.
PS00107	; PROTEIN_KINASE_ATP; 1.
PS50011	; PROTEIN_KINASE_DOM; 1.
PS00109	; PROTEIN_KINASE_TYR; 1.
PS00240	; RECEPTOR_TYR_KIN_III; 1.

Prints

Created Date

20-Feb-2013