EKS-CAF-00126

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EKS-CAF-00126

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
TK/Met	478.6	5.9E-144	1050	1310	261

Status

Reviewed

Ensembl Protein

ENSCAFP00000005067

UniProt Accession

Q75ZY9; A0M8U9;

Protein Name

Hepatocyte growth factor receptor

Protein Synonyms/Alias

HGF receptor; HGF/SF receptor; Proto-oncogene c-Met; Scatter factor receptor; SF receptor; Tyrosine-protein kinase Met;

Gene Name

MET

Gene Synonyms/Alias

MET;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
ENSCAFG00000003406	ENSCAFP00000041478	ENSCAFT00000049462
ENSCAFG00000003406	ENSCAFP00000005067	ENSCAFT00000005469

Organism

Canis familiaris

Functional Description

Receptor tyrosine kinase that transduces signals fromthe extracellular matrix into the cytoplasm by binding to hepatocyte growth factor/HGF ligand. Regulates many physiological processes including proliferation, scattering, morphogenesis and survival. Ligand binding at the cell surface induces autophosphorylation of MET on its intracellular domain that provides docking sites for downstream signaling molecules. Following activation by ligand, interacts with the PI3-kinase subunit PIK3R1, PLCG1, SRC, GRB2, STAT3 or the adapter GAB1. Recruitment of these downstream effectors by MET leads to the activation of several signaling cascades including the RAS-ERK, PI3 kinase-AKT, or PLCgamma-PKC. The RAS-ERK activation is associated with the morphogenetic effects while PI3K/AKT coordinates prosurvival effects. During embryonic development, MET signaling plays a role in gastrulation, development and migration of muscles and neuronal precursors, angiogenesis and kidney formation. In adults, participates in wound healing as well as organ regeneration and tissue remodeling. Promotes also differentiation and proliferation of hematopoietic cells (By similarity).

Protein Length

1353

Protein Sequence
(FASTA)

MKAPAVLAPG ILVLLFTLVQ KSYGECKEAL VKSEMNVNMK YQLPNFTAET PIQNVVLHKH 60

HIYLGAVNYI YVLNDKDLQK VAEYKTGPVL EHPDCSPCQD CSHKANLSGG VWEDNINMAL 120

LVDTYYDDQL ISCGSVHRGT CQRHILPPSN IADIQSEVHC MYSSQADEEP SQCPDCVVSA 180

LGTKVLISEK DRFINFFVGN TINSSDHPDH SLHSISVRRL KETQDGFKFL TDQSYIDVLP 240

EFRDSYPIKY VHAFESNHFI YFLTVQRETL DAQTFHTRII RFCSVDSGLH SYMEMPPGAH 300

LAKQIGANLN DDILYGVFAQ SKPDSAEPMN RSAVCAFPIK YVNEFFNKIV NKNNVRCLQH 360

FYGPNHEHCF NRTLLRNSSG CEARNDEYRT EFTTALQRVD LFMGQFNQVL LTSISTFIKG 420

DLTIANLGTS EGRFMQVVVS RSGLSTPHVN FRLDSHPVSP EAIVEHPLNQ NGYTLVVTGK 480

KITRIPLNGL GCEHFQSCSQ CLSAPPFVQC GWCHDRCVHL EECPTGAWTQ EVCLPAIYEV 540

FPTSAPLEGG TVLTVCGWDF GFRRNNKFDL KKTKVFLGNE SCTLTLSEST TNMLKCTVGP 600

AVNEHFNISI IISNGRGTAQ YSTFSYVDPI ITSISPSYGP KNGGTLLTLT GKYLNSGNSR 660

HISMGGKTCT LKSVSDSILE CYTPAQATAT EFPIKLKIDL ANREMNSFSY QEDPIVYAIH 720

PTKSFISGGS TITAVGKNLN SVSVLRMVID VHETRRNFTV ACQHRSNSEI ICCTTPSLQQ 780

LNLQLPLKTK AFFMLDGIHS KYFDLIYVHN PVFKPFEKPV MISIGNENVL EIKGNDIDPE 840

AVKGEVLKVG NKSCETIYSD SKAVLCKVPN DLLKLNNELN IEWKQAVSST VLGKVIVQPD 900

QNFTGLIAGV ISISTIVLLL LGLFLWLKRK KQIKDLGSEL VRYDARVHTP HLDRLVSARS 960

VSPTTEMVSN ESVDYRATFP EDQFPNSSQN GSCRQVQYPL TDLSPMLTSG DSDISSPLLQ 1020

NTVHIDLSAL NPELVQAVQH VVIGPSSLIV HFNEVIGRGH FGCVYHGTLL DNDDKKIHCA 1080

VKSLNRITDI GEVSQFLTEG IIMKDFSHPN VLSLLGICLR SEGSPLVVLP YMKHGDLRNF 1140

IRNETHNPTV KDLIGFGLQV AKGMKYLASK KFVHRDLAAR NCMLDEKFTV KVADFGLARD 1200

MYDKEYYSVH NKTGAKLPVK WMALESLQTQ KFTTKSDVWS FGVLLWELMT RGAPPYPDVN 1260

TFDITVYLLQ GRRLLQPEYC PDPLYEVMLK CWHPRAELRP SFSELVSRIS AIFSTFIGEH 1320

YVHVNATYVN VKCVAPYPSL LSSQDNIDGE GDT 1353

Nucleotide Sequence
(FASTA)

ATGAAGGCTC CTGCTGTGCT TGCACCTGGC ATCCTTGTGC TTCTGTTTAC CTTGGTGCAG 60

AAGAGCTATG GGGAGTGCAA AGAGGCACTA GTAAAGTCTG AGATGAATGT GAACATGAAG 120

TATCAGCTTC CCAACTTCAC TGCCGAAACA CCCATCCAGA ATGTTGTTTT ACACAAGCAT 180

CATATTTACC TTGGTGCAGT TAACTATATT TACGTTTTAA ATGACAAAGA CCTTCAGAAG 240

GTTGCTGAGT ACAAGACTGG GCCCGTGCTG GAACACCCAG ATTGTTCCCC ATGTCAGGAC 300

TGCAGCCACA AAGCCAATTT ATCAGGTGGT GTTTGGGAAG ATAACATCAA CATGGCTCTG 360

CTTGTTGACA CATACTACGA TGACCAACTC ATTAGCTGTG GCAGTGTCCA CAGAGGGACC 420

TGCCAGCGAC ATATCCTTCC ACCCAGCAAT ATTGCTGACA TACAGTCGGA AGTGCATTGC 480

ATGTACTCCT CACAGGCAGA CGAAGAGCCC AGCCAGTGCC CTGACTGTGT GGTGAGTGCT 540

CTAGGAACCA AAGTCCTGAT ATCAGAAAAG GACCGGTTCA TCAACTTCTT CGTAGGCAAT 600

ACCATAAATT CCTCGGACCA TCCAGATCAT TCATTGCATT CGATATCGGT GAGAAGGCTA 660

AAGGAAACGC AAGATGGGTT CAAGTTTTTG ACAGACCAGT CTTACATTGA TGTTCTACCG 720

GAGTTCAGAG ACTCCTACCC CATTAAATAT GTCCACGCCT TTGAAAGCAA CCACTTTATT 780

TACTTTTTGA CAGTCCAGCG AGAAACTCTA GATGCTCAGA CTTTTCACAC GAGAATAATC 840

AGGTTCTGTT CTGTAGACTC TGGATTGCAT TCCTACATGG AAATGCCTCC TGGGGCCCAT 900

CTCGCTAAAC AAATAGGTGC CAACCTGAAT GATGACATTC TCTATGGAGT GTTCGCACAA 960

AGCAAGCCAG ATTCTGCTGA ACCAATGAAT CGCTCTGCCG TCTGTGCGTT CCCTATCAAA 1020

TATGTCAATG AATTCTTCAA CAAGATCGTC AACAAAAACA ATGTGAGATG TCTTCAGCAC 1080

TTTTATGGAC CCAACCACGA ACACTGCTTT AATAGGACAC TTTTGAGAAA TTCATCGGGC 1140

TGTGAAGCGC GCAATGATGA ATATCGAACG GAGTTCACTA CAGCTTTGCA GCGCGTTGAC 1200

TTATTCATGG GCCAGTTCAA CCAAGTCCTC TTAACGTCTA TATCCACCTT CATCAAAGGA 1260

GACCTCACCA TTGCTAATCT TGGGACGTCC GAGGGTCGCT TCATGCAGGT CGTGGTTTCT 1320

CGATCAGGAT TGTCGACCCC TCACGTGAAC TTCCGCCTGG ACTCCCACCC CGTGTCTCCA 1380

GAAGCAATTG TGGAGCACCC ACTAAACCAA AACGGCTACA CACTCGTTGT CACTGGGAAG 1440

AAGATCACCA GGATCCCACT GAATGGCTTA GGCTGTGAGC ATTTTCAGTC CTGCAGTCAG 1500

TGTCTCTCCG CCCCTCCCTT TGTGCAGTGT GGCTGGTGCC ACGATAGATG TGTGCACCTG 1560

GAGGAATGTC CCACTGGAGC GTGGACTCAG GAGGTCTGTC TGCCTGCAAT CTATGAGGTT 1620

TTCCCAACTA GTGCACCCCT GGAAGGAGGG ACAGTGCTGA CTGTATGTGG CTGGGACTTC 1680

GGATTCAGGA GGAATAATAA ATTTGATTTA AAGAAAACCA AAGTTTTCCT TGGAAATGAG 1740

AGCTGCACCT TGACCTTAAG TGAGAGCACA ACAAATATGC TGAAATGCAC AGTTGGCCCT 1800

GCAGTGAACG AGCATTTCAA TATATCCATA ATTATTTCAA ATGGTCGAGG GACAGCACAA 1860

TATAGTACAT TTTCGTATGT GGATCCTATT ATAACAAGTA TTTCTCCAAG TTATGGTCCC 1920

AAGAATGGTG GCACCTTGCT CACTTTAACT GGAAAATACC TCAACAGTGG GAATTCTAGA 1980

CACATTTCAA TGGGTGGAAA AACATGTACT TTAAAAAGTG TGTCAGATAG TATTCTCGAA 2040

TGTTATACCC CAGCTCAAGC CACTGCAACT GAGTTTCCTA TTAAATTGAA AATTGACCTA 2100

GCCAACCGAG AGATGAACAG CTTCAGTTAC CAGGAAGACC CCATTGTCTA TGCAATTCAT 2160

CCAACGAAAT CTTTTATTAG TGGTGGGAGC ACAATAACAG CTGTTGGAAA AAACCTGAAT 2220

TCAGTGAGTG TCCTGAGGAT GGTAATAGAT GTCCATGAAA CAAGAAGGAA CTTTACAGTG 2280

GCATGTCAAC ATCGCTCTAA TTCAGAGATA ATCTGTTGTA CGACTCCTTC ACTGCAACAG 2340

CTGAATCTGC AACTCCCTCT GAAAACCAAA GCCTTTTTCA TGTTAGATGG GATCCATTCC 2400

AAATACTTTG ATCTCATTTA TGTACATAAT CCTGTGTTTA AGCCTTTTGA AAAGCCAGTG 2460

ATGATCTCAA TAGGCAATGA AAATGTACTG GAAATTAAGG GAAATGATAT TGACCCTGAA 2520

GCAGTTAAAG GCGAAGTGTT AAAAGTTGGA AATAAGAGCT GTGAGACTAT CTACTCAGAT 2580

TCTAAAGCCG TTTTATGCAA GGTCCCCAAT GACCTGCTGA AATTGAACAA CGAGCTAAAT 2640

ATAGAGTGGA AGCAAGCAGT TTCTTCAACC GTCCTTGGAA AAGTAATAGT TCAACCAGAT 2700

CAGAATTTCA CAGGATTGAT TGCTGGTGTT ATCTCAATAT CAACAATAGT CTTATTATTA 2760

CTCGGACTTT TCCTGTGGCT GAAAAGGAAA AAGCAAATTA AAGATCTGGG CAGTGAATTA 2820

GTTCGCTATG ATGCAAGAGT ACACACTCCT CATTTGGATA GGCTTGTAAG TGCCCGAAGT 2880

GTAAGCCCAA CTACAGAAAT GGTTTCAAAT GAATCTGTAG ACTACCGAGC TACTTTTCCA 2940

GAAGACCAGT TTCCTAATTC ATCTCAGAAT GGATCATGCA GACAAGTACA ATATCCTCTG 3000

ACGGACCTGT CCCCCATGCT TACTAGTGGG GACTCTGATA TATCCAGTCC ATTATTGCAA 3060

AATACTGTCC ACATTGACCT CAGTGCTCTA AATCCAGAGC TGGTGCAGGC AGTCCAGCAT 3120

GTAGTGATTG GGCCCAGTAG CCTGATTGTG CATTTCAATG AAGTCATAGG AAGAGGACAT 3180

TTTGGGTGTG TATACCATGG GACTTTGTTG GACAATGACG ACAAAAAAAT TCACTGTGCT 3240

GTGAAATCCC TGAATAGAAT CACTGACATA GGAGAAGTTT CCCAGTTTCT GACCGAGGGA 3300

ATCATCATGA AAGATTTTAG TCATCCAAAC GTACTCTCAC TCTTGGGAAT CTGCCTTCGA 3360

AGTGAGGGGT CTCCACTGGT GGTCCTACCA TACATGAAAC ATGGAGATCT TCGAAATTTC 3420

ATTAGAAATG AGACTCATAA CCCAACTGTA AAAGATCTTA TTGGCTTTGG TCTTCAAGTA 3480

GCCAAAGGCA TGAAATATCT TGCAAGCAAA AAGTTTGTCC ACAGAGACTT GGCTGCAAGA 3540

AACTGTATGC TGGATGAAAA ATTCACTGTC AAGGTTGCTG ATTTTGGTCT TGCCAGAGAC 3600

ATGTATGATA AAGAATACTA CAGCGTACAC AACAAAACAG GCGCCAAACT ACCAGTGAAG 3660

TGGATGGCTT TAGAAAGTCT GCAAACTCAG AAGTTTACCA CCAAGTCAGA TGTGTGGTCC 3720

TTTGGCGTGC TCCTCTGGGA ACTGATGACA AGAGGAGCAC CACCTTATCC TGACGTCAAC 3780

ACCTTTGATA TAACAGTTTA CTTGTTGCAA GGCAGAAGGC TCCTGCAACC CGAATACTGC 3840

CCAGATCCCT TATACGAAGT GATGCTAAAA TGCTGGCACC CTAGAGCTGA ACTGCGCCCA 3900

TCTTTTTCTG AACTGGTCTC CAGGATATCA GCAATATTCT CTACTTTCAT TGGGGAGCAC 3960

TATGTCCATG TGAACGCCAC TTATGTGAAT GTCAAATGTG TTGCTCCATA CCCTTCTCTC 4020

TTGTCATCAC AAGATAACAT TGATGGCGAG GGGGACACAT GA 4062

Domain Profile

S: 1     vdkeeviGkGhfGvvykgelsdsaskkikvavksleritdiekveeflreglvmkeldhp  60

         v+++eviG+GhfG+vy+g+l+d+++kki++avksl+ritdi++v++fl+eg++mk+++hp

Q: 1050  VHFNEVIGRGHFGCVYHGTLLDNDDKKIHCAVKSLNRITDIGEVSQFLTEGIIMKDFSHP  1109

         799*********************************************************

S: 61    nvlsllGialddeglplvvlpymkkGdlksfirneernltvkdllefalqvakgmeylas  120

         nvlsllGi+l++eg+plvvlpymk+Gdl++firne++n+tvkdl++f+lqvakgm+ylas

Q: 1110  NVLSLLGICLRSEGSPLVVLPYMKHGDLRNFIRNETHNPTVKDLIGFGLQVAKGMKYLAS  1169

         ************************************************************

S: 121   kkfvhrdlaarnclldekltvkvadfGlardvldkelyvveeerdarlpvkwlaleslqt  180

         kkfvhrdlaarnc+ldek+tvkvadfGlard++dke+y+v+++++a+lpvkw+aleslqt

Q: 1170  KKFVHRDLAARNCMLDEKFTVKVADFGLARDMYDKEYYSVHNKTGAKLPVKWMALESLQT  1229

         ************************************************************

S: 181   fkqfttksdvwsfGvllwelltrgatpysevasfdltlylkegrrlrkpeycpdklydvm  240

         +k fttksdvwsfGvllwel+trga+py++v++fd+t+yl +grrl +peycpd ly+vm

Q: 1230  QK-FTTKSDVWSFGVLLWELMTRGAPPYPDVNTFDITVYLLQGRRLLQPEYCPDPLYEVM  1288

         **.*********************************************************

S: 241   lkcwkakpaerpqfsdlvsiis  262

         lkcw++ ++ rp+fs+lvs+is

Q: 1289  LKCWHPRAELRPSFSELVSRIS  1310

         ********************98

Domain Sequence
(FASTA)

VHFNEVIGRG HFGCVYHGTL LDNDDKKIHC AVKSLNRITD IGEVSQFLTE GIIMKDFSHP 60

NVLSLLGICL RSEGSPLVVL PYMKHGDLRN FIRNETHNPT VKDLIGFGLQ VAKGMKYLAS 120

KKFVHRDLAA RNCMLDEKFT VKVADFGLAR DMYDKEYYSV HNKTGAKLPV KWMALESLQT 180

QKFTTKSDVW SFGVLLWELM TRGAPPYPDV NTFDITVYLL QGRRLLQPEY CPDPLYEVML 240

KCWHPRAELR PSFSELVSRI S 261

Keyword

ATP-binding; Complete proteome; Disulfide bond; Glycoprotein; Kinase; Membrane; Nucleotide-binding; Phosphoprotein; Proto-oncogene; Receptor; Reference proteome; Repeat; Signal; Transferase; Transmembrane; Transmembrane helix; Tyrosine-protein kinase; Ubl conjugation.

Sequence Source

Ensembl

Orthology

Ortholog group

Ailuropoda melanoleuca"; ?>Anolis carolinensis"; ?>Bos taurus"; ?>Caenorhabditis elegans"; ?>Callithrix jacchus"; ?>Cavia porcellus"; ?>Ciona intestinalis"; ?>Ciona savignyi"; ?>Danio rerio"; ?>Equus caballus"; ?>Felis catus"; ?>Gallus gallus"; ?>Gasterosteus aculeatus"; ?>Gorilla gorilla"; ?>Homo sapiens"; ?>Ictidomys tridecemlineatus"; ?>Latimeria chalumnae"; ?>Loxodonta africana"; ?>Macaca mulatta"; ?>Meleagris gallopavo"; ?>Monodelphis domestica"; ?>Mus musculus"; ?>Mustela putorius furo"; ?>Myotis lucifugus"; ?>Nomascus leucogenys"; ?>Ochotona princeps"; ?>Oreochromis niloticus"; ?>Ornithorhynchus anatinus"; ?>Oryctolagus cuniculus"; ?>Oryzias latipes"; ?>Otolemur garnettii"; ?>Pan troglodytes"; ?>Pelodiscus sinensis"; ?>Petromyzon marinus"; ?>Pongo abelii"; ?>Rattus norvegicus"; ?>Sarcophilus harrisii"; ?>Sus scrofa"; ?>Taeniopygia guttata"; ?>Takifugu rubripes"; ?>Tetraodon nigroviridis"; ?>Vicugna pacos"; ?>Xenopus tropicalis"; ?>

Gene Ontology

GO:0016021	; C:integral to membrane
GO:0005524	; F:ATP binding
GO:0004714	; F:transmembrane receptor protein tyrosine kinase activity
GO:0007275	; P:multicellular organismal development
GO:0007169	; P:transmembrane receptor protein tyrosine kinase signaling pathway

KEGG

cfa:403438

;

InterPros

IPR013783	; Ig-like_fold.
IPR014756	; Ig_E-set.
IPR002909	; IPT_TIG_rcpt.
IPR011009	; Kinase-like_dom.
IPR003659	; Plexin-like.
IPR016201	; Plexin-like_fold.
IPR002165	; Plexin_repeat.
IPR000719	; Prot_kinase_cat_dom.
IPR017441	; Protein_kinase_ATP_BS.
IPR001627	; Semaphorin/CD100_Ag.
IPR001245	; Ser-Thr/Tyr_kinase_cat_dom.
IPR008266	; Tyr_kinase_AS.
IPR020635	; Tyr_kinase_cat_dom.
IPR016244	; Tyr_kinase_HGF/MSP_rcpt.
IPR015943	; WD40/YVTN_repeat-like_dom.

Pfam

PF07714	; Pkinase_Tyr; 1.
PF01437	; PSI; 1.
PF01403	; Sema; 1.
PF01833	; TIG; 3.

SMARTs

SM00429	; IPT; 4.
SM00423	; PSI; 1.
SM00630	; Sema; 1.
SM00219	; TyrKc; 1.

Prosites

PS00107	; PROTEIN_KINASE_ATP; 1.
PS50011	; PROTEIN_KINASE_DOM; 1.
PS00109	; PROTEIN_KINASE_TYR; 1.
PS51004	; SEMA; 1.

Prints

PR00109

; TYRKINASE.

Created Date

20-Feb-2013