EKS-CAF-00211

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EKS-CAF-00211

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
AGC/MAST	352.7	1.7E-105	35	312	278

Status

Reviewed

Ensembl Protein

ENSCAFP00000006520

UniProt Accession

E2RJI4;

Protein Name

Serine/threonine-protein kinase greatwall

Protein Synonyms/Alias

GW; GWL; Microtubule-associated serine/threonine-protein kinase-like; MAST-L;

Gene Name

MASTL

Gene Synonyms/Alias

MASTL; GW, GWL;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
ENSCAFG00000004382	ENSCAFP00000038134	ENSCAFT00000045083
ENSCAFG00000004382	ENSCAFP00000006520	ENSCAFT00000007043

Organism

Canis familiaris

Functional Description

Serine/threonine kinase that plays a key role in M phaseby acting as a regulator of mitosis entry and maintenance. Acts by promoting the inactivation of protein phosphatase 2A (PP2A) during M phase: does not directly inhibit PP2A but acts by mediating phosphorylation and subsequent activation of ARPP19 and ENSA at 'Ser-62' and 'Ser-67', respectively. ARPP19 and ENSA are phosphatase inhibitors that specifically inhibit the PPP2R2D (PR55-delta) subunit of PP2A. Inactivation of PP2A during M phase is essential to keep cyclin-B1-CDK1 activity high. Following DNA damage, it is also involved in checkpoint recovery by being inhibited (By similarity).

Protein Length

883

Protein Sequence
(FASTA)

MEATAGSETE SGGDTVTAEC ANRIPVPRPP SIEEFTIVKP ISRGAFGKVY LGQKGGKLYA 60

VKVVKKADMI NKNMTHQVQA ERDALALSKS PFIVHLYYSL QSANNVYLVM EYLIGGDVKS 120

LLHIYGYFDE EMAVKYISEV ALALDYLHRH GIIHRDLKPD NMLISNEGHI KLTDFGLSKI 180

TLNRDIDINM MDILTTPSMA KPRQDYSRTP GQVLSLISSL RFYTPAAEKD KDSANILSTH 240

VFETSQLSQG LICPMSVDHR DTTPYSSKLL HSCLETVTSD PGMPVKCLTS NLLQSRRRLA 300

TSSASSQSHT FLSSVESECH SSPRWEKDCQ ESDDALGSTV MSWNIIEKSS CTKSTDAIET 360

KGFNKKDFEL TLSPIHNSSI IPATGSSCVN LAKKCFPGEV SWEARELDIN NVNVATDTTR 420

CVFHQSDQWV VDPSDGTEEY CGKRGFKRNS ELVDSSPCQN IIQNKKNCIE HKSRNEKSNG 480

YINQRTSLTN EVQDLKLSVC ESQQSDCANK ENMVNSSIDK QQTPEKSPIP MIAKNLMCEL 540

DEDCNKNNKK FLSSSFLGSD DERASKSICM DSDSSFPGIS IMESSLERQS LDPDKSIRES 600

SFEESNIEDL LAVSPSWQEN PLPKDDENLA VQASSQKMLA SSSDVLKTLT LSKRNAVAFR 660

SFNSHINASN NSEPSKMSVT SLDAMDISCV YSGSYPMAIT PSQKGISYVP YQQTPNQVKS 720

ETPYRTPKSV RRGAAPVDDA RILGTPDYLA PELLLGRAHG PAVDWWALGV CLFEFLTGIP 780

PFNDETSQQV FQNILKRDIP WPEGEEKLSD NAQNAVEILL TIDNAKRAGI KELKCHPLFS 840

DVDWENLQHQ TMPFIPQPDD ETDTSYFEAR NNAQHLTVSG FSL 883

Nucleotide Sequence
(FASTA)

ATGGAGGCCA CTGCGGGGAG CGAGACGGAG AGTGGCGGAG ACACGGTTAC AGCGGAGTGC 60

GCGAACAGGA TCCCGGTGCC AAGACCTCCC TCCATTGAGG AATTCACCAT AGTAAAACCC 120

ATTAGCCGTG GCGCTTTTGG AAAGGTGTAT CTCGGGCAGA AAGGCGGCAA GTTGTATGCT 180

GTGAAGGTTG TCAAAAAAGC AGATATGATC AACAAAAATA TGACTCATCA GGTACAAGCT 240

GAGAGGGATG CACTAGCACT AAGCAAAAGT CCTTTCATTG TCCATTTGTA CTATTCACTA 300

CAGTCAGCAA ACAATGTCTA CTTGGTAATG GAATATCTTA TTGGTGGAGA TGTCAAGTCT 360

CTCCTACATA TATATGGTTA CTTTGATGAA GAGATGGCTG TGAAATATAT TTCTGAAGTA 420

GCCTTGGCTC TGGACTATCT TCACAGACAT GGAATCATCC ACAGGGATTT GAAACCAGAC 480

AATATGCTTA TTTCAAATGA AGGTCATATT AAACTAACAG ATTTTGGGCT TTCCAAAATT 540

ACTTTGAATA GAGACATAGA TATCAATATG ATGGATATCC TTACAACACC GTCAATGGCT 600

AAACCTAGAC AAGATTATTC AAGAACCCCA GGACAAGTGT TATCTCTCAT CAGCTCTTTA 660

AGATTTTACA CACCAGCTGC AGAAAAAGAC AAAGACTCTG CAAATATTCT TTCAACCCAT 720

GTATTTGAAA CATCACAGCT TTCTCAAGGA CTAATTTGTC CCATGTCTGT AGATCACAGG 780

GATACCACTC CTTATTCTAG CAAACTACTA CACTCATGTC TTGAAACAGT TACCTCCGAT 840

CCTGGGATGC CTGTGAAGTG CCTGACATCT AATCTACTGC AGTCCAGGAG AAGGCTGGCT 900

ACATCAAGTG CCAGCAGTCA GAGCCACACC TTCCTGTCCA GTGTGGAGTC TGAGTGCCAC 960

AGCAGTCCCA GATGGGAAAA AGACTGCCAG GAAAGTGATG ATGCACTGGG CTCTACAGTG 1020

ATGAGTTGGA ATATAATAGA AAAGTCTTCG TGTACAAAAT CTACCGATGC CATTGAGACA 1080

AAAGGTTTCA ATAAAAAGGA TTTTGAATTA ACCCTTTCTC CTATTCATAA CAGCAGTATC 1140

ATTCCTGCGA CTGGAAGCTC TTGTGTAAAC CTGGCTAAAA AATGCTTCCC TGGAGAAGTT 1200

TCTTGGGAAG CAAGAGAACT GGACATAAAT AATGTTAATG TGGCCACTGA CACCACCCGG 1260

TGTGTTTTCC ATCAATCGGA TCAGTGGGTT GTGGATCCCA GTGATGGGAC TGAAGAATAC 1320

TGTGGAAAAA GAGGTTTTAA AAGAAATTCT GAGTTAGTTG ACTCTAGTCC TTGTCAGAAC 1380

ATCATACAGA ATAAAAAAAA TTGTATAGAG CATAAGTCTA GAAATGAAAA AAGTAATGGT 1440

TATATAAATC AAAGGACAAG CTTAACCAAT GAAGTCCAAG ACCTGAAGCT ATCGGTATGT 1500

GAAAGTCAAC AAAGTGACTG TGCCAATAAG GAGAACATGG TCAATTCTTC CATTGATAAA 1560

CAACAAACCC CAGAAAAATC ACCTATCCCA ATGATAGCAA AAAACCTTAT GTGTGAATTG 1620

GATGAAGATT GCAACAAGAA TAATAAGAAG TTCTTAAGTT CTAGCTTTCT GGGTTCTGAT 1680

GATGAGAGAG CTTCTAAAAG TATCTGTATG GACTCTGATT CATCTTTTCC TGGAATTTCT 1740

ATAATGGAAA GTTCATTAGA AAGGCAGTCC TTAGATCCAG ATAAAAGCAT CAGAGAATCT 1800

TCTTTTGAAG AATCAAACAT TGAAGACCTA CTTGCTGTAT CACCCAGCTG GCAAGAAAAT 1860

CCCTTGCCAA AAGATGATGA GAATCTGGCT GTCCAAGCCA GTAGCCAAAA AATGTTAGCT 1920

TCGTCTTCTG ATGTGTTAAA AACATTAACC TTATCTAAAA GAAATGCAGT AGCTTTTCGA 1980

AGTTTTAACA GTCATATTAA TGCGTCCAAT AACTCAGAAC CATCCAAAAT GAGTGTTACC 2040

TCTTTAGATG CAATGGATAT TTCCTGTGTT TATAGTGGTT CATATCCCAT GGCTATAACC 2100

CCTAGCCAAA AAGGAATATC CTATGTGCCA TATCAGCAGA CCCCAAATCA GGTCAAATCA 2160

GAAACTCCAT ACCGAACTCC AAAGAGTGTA AGGCGAGGAG CGGCCCCAGT AGATGATGCG 2220

CGAATTCTTG GAACCCCGGA CTACCTTGCT CCTGAGCTGT TACTGGGCAG GGCCCATGGT 2280

CCTGCGGTAG ACTGGTGGGC ACTTGGAGTT TGCTTGTTTG AGTTTCTTAC AGGAATTCCT 2340

CCTTTCAATG ATGAAACATC ACAGCAAGTA TTCCAGAATA TTCTGAAAAG AGATATCCCT 2400

TGGCCTGAAG GTGAAGAAAA GTTATCAGAT AATGCTCAAA ATGCGGTAGA AATACTTTTA 2460

ACTATTGATA ATGCAAAAAG AGCTGGAATT AAGGAGCTGA AATGTCATCC TCTCTTCAGT 2520

GACGTGGACT GGGAAAATCT GCAGCATCAA ACTATGCCTT TCATACCTCA ACCAGATGAC 2580

GAAACAGATA CATCCTATTT TGAAGCCAGG AATAATGCTC AGCACCTGAC CGTATCTGGA 2640

TTTAGTCTAT AG 2652

Domain Profile

S: 1     feiiklisrGayGavylvrhketrkrfavkkikkqdlilknqvqqvfaerdilalaenpf  60

         f+i+k+isrGa+G+vyl  +++++k++avk++kk+d+i+kn+++qv+aerd+lal+++pf

Q: 35    FTIVKPISRGAFGKVYL--GQKGGKLYAVKVVKKADMINKNMTHQVQAERDALALSKSPF  92

         89***************..*****************************************

S: 61    vvslyysletrrnlylvmeyveGGdvksllkniGaldvemarlyiaevvlaleylhryGi  120

         +v+lyysl++++n+ylvmey++GGdvksll+++G++d+ema++yi+ev+lal+ylhr+Gi

Q: 93    IVHLYYSLQSANNVYLVMEYLIGGDVKSLLHIYGYFDEEMAVKYISEVALALDYLHRHGI  152

         ************************************************************

S: 121   vhrdlkPdnllitseGhikltdfGlskvglmslttnlyegyidkdarefldkqvcgtPey  180

         +hrdlkPdn+li++eGhikltdfGlsk++l+          id++++++l     +tP++

Q: 153   IHRDLKPDNMLISNEGHIKLTDFGLSKITLN--------RDIDINMMDIL-----TTPSM  199

         *******************************........67999999999.....*****

S: 181   iaPevilrqgygkpvdwwalgiilyeflvgivpffgdtpeelfaqv.....lsdeivwP.  234

         ++P    rq+y++++++++++i+++ f+++ +++++d+++ l+++v     ls+++++P

Q: 200   AKP----RQDYSRTPGQVLSLISSLRFYTPAAEKDKDSANILSTHVfetsqLSQGLICPm  255

         ***....*************************************9999999********9

S: 235   ...ekde.alssealelisellesnP..............rerlgtgsaaevkqhefl  274

            ++d+ ++ss++l+++ e+++s+P              r+rl+t s+a++++h+fl

Q: 256   svdHRDTtPYSSKLLHSCLETVTSDPgmpvkcltsnllqsRRRLAT-SSASSQSHTFL  312

         998666679******************************999****.888999**996

Domain Sequence
(FASTA)

FTIVKPISRG AFGKVYLGQK GGKLYAVKVV KKADMINKNM THQVQAERDA LALSKSPFIV 60

HLYYSLQSAN NVYLVMEYLI GGDVKSLLHI YGYFDEEMAV KYISEVALAL DYLHRHGIIH 120

RDLKPDNMLI SNEGHIKLTD FGLSKITLNR DIDINMMDIL TTPSMAKPRQ DYSRTPGQVL 180

SLISSLRFYT PAAEKDKDSA NILSTHVFET SQLSQGLICP MSVDHRDTTP YSSKLLHSCL 240

ETVTSDPGMP VKCLTSNLLQ SRRRLATSSA SSQSHTFL 278

Keyword

ATP-binding; Cell cycle; Cell division; Complete proteome; Cytoplasm; Cytoskeleton; Kinase; Mitosis; Nucleotide-binding; Nucleus; Phosphoprotein; Reference proteome; Serine/threonine-protein kinase; Transferase.

Sequence Source

Ensembl

Orthology

Ortholog group

Ailuropoda melanoleuca"; ?>Anolis carolinensis"; ?>Bos taurus"; ?>Callithrix jacchus"; ?>Cavia porcellus"; ?>Ciona intestinalis"; ?>Ciona savignyi"; ?>Danio rerio"; ?>Drosophila melanogaster"; ?>Equus caballus"; ?>Felis catus"; ?>Gallus gallus"; ?>Gasterosteus aculeatus"; ?>Gorilla gorilla"; ?>Homo sapiens"; ?>Ictidomys tridecemlineatus"; ?>Latimeria chalumnae"; ?>Loxodonta africana"; ?>Macaca mulatta"; ?>Meleagris gallopavo"; ?>Monodelphis domestica"; ?>Mus musculus"; ?>Mustela putorius furo"; ?>Myotis lucifugus"; ?>Nomascus leucogenys"; ?>Oreochromis niloticus"; ?>Ornithorhynchus anatinus"; ?>Oryctolagus cuniculus"; ?>Oryzias latipes"; ?>Otolemur garnettii"; ?>Pan troglodytes"; ?>Pelodiscus sinensis"; ?>Petromyzon marinus"; ?>Pongo abelii"; ?>Procavia capensis"; ?>Pteropus vampyrus"; ?>Rattus norvegicus"; ?>Sarcophilus harrisii"; ?>Sorex araneus"; ?>Sus scrofa"; ?>Taeniopygia guttata"; ?>Takifugu rubripes"; ?>Tetraodon nigroviridis"; ?>Xenopus tropicalis"; ?>Xiphophorus maculatus"; ?>Saccharomyces cerevisiae"; ?>Schizosaccharomyces pombe"; ?>

Gene Ontology

GO:0005813	; C:centrosome
GO:0032154	; C:cleavage furrow
GO:0005634	; C:nucleus
GO:0005524	; F:ATP binding
GO:0051721	; F:protein phosphatase 2A binding
GO:0004674	; F:protein serine/threonine kinase activity
GO:0051301	; P:cell division
GO:0000086	; P:G2/M transition of mitotic cell cycle
GO:0007067	; P:mitosis
GO:0034048	; P:negative regulation of protein phosphatase type 2A activity
GO:0051726	; P:regulation of cell cycle
GO:0006974	; P:response to DNA damage stimulus

KEGG

InterPros

IPR000961	; AGC-kinase_C.
IPR011009	; Kinase-like_dom.
IPR000719	; Prot_kinase_cat_dom.
IPR008271	; Ser/Thr_kinase_AS.

Pfam

PF00069

; Pkinase; 2.

SMARTs

SM00133

; S_TK_X; 1.

Prosites

PS51285	; AGC_KINASE_CTER; 1.
PS00107	; PROTEIN_KINASE_ATP; FALSE_NEG.
PS50011	; PROTEIN_KINASE_DOM; 1.
PS00108	; PROTEIN_KINASE_ST; 1.

Prints

Created Date

20-Feb-2013