EKS-CAF-00347

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EKS-CAF-00347

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
Other/PEK	363.5	9.7E-109	590	1004	415

Status

Unreviewed

Ensembl Protein

ENSCAFP00000012980

UniProt Accession

E2RS10;

Protein Name

Protein Synonyms/Alias

Gene Name

EIF2AK4

Gene Synonyms/Alias

EIF2AK4;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
ENSCAFG00000008775	ENSCAFP00000012980	ENSCAFT00000014032

Organism

Canis familiaris

Functional Description

Protein Length

1651

Protein Sequence
(FASTA)

MAGGRGAPGR GRDEPPESYP QRQEHELQAL EAIYGADFQD LRPDARKSVN EPPEINLVLY 60

PQGLTGEEVY VQVDLRVKCP PTYPDVVPEI ELKNAKGLSN ESVHLLKSHL EKVAKKHCGE 120

VMIFELADHV QSFLSEHNKP PPKSFHEEML ERRAQEEQRR LLEAKQKVEQ EQREILHEIQ 180

RRKEEIKEER KRKEMAKQER LEIASLSNQD QNSRKDPGGH RISAILHGGS PDFVGNGKHR 240

TNSSGRSRRE RQYSLCSSED SSGSCEVLYF SMGSPDQLMV YKGRCIGSDE QLGKLVYNAL 300

ETATGNFVSL HEWVLRWQKK MGPFLTSQEK EKIDKCKKQI QGAETEFNSL VKLSHPNVVR 360

YFAMNLKEQD DSIMVDILLE HINGVSLSAH LSHSGPIPVY QLRKYTAQLL SGLDYLHSND 420

VVHKVLNASN VLVDAEGNVK ITDYSISKRL ADICKEDVFE QSRVSFSDSA LPYKTGKKGD 480

VWCLGLLLLF LSQGQECGEY PVTIPSDLPA DFQDFLKKCV CLDDKERWSP QQLLKHSFIN 540

PQPKMSLLEQ SPDDSGGQDY VETVIPSKQL PSAAFFSETQ RQFSRYFIEF EELQLLGKGA 600

FGAVIKVQNK LDGCCYAVKR IPINPASRHF RRIKGEVTLL SRLHHENIVR YYNAWIERYE 660

RPVGPGMLPQ DSDSWPQAPD GRALSQPSPG NDSPDGDGAG SVEAAAPPPI LSSVEWSTSG 720

ERSASARFPS ARPGSSSEED DDDDEHEGVF SQPFLPASDS DSDVIFEDEN SKSQNQEEDC 780

NGKNSCHESE PSTTTEAVHY LYIQMEYCEK STLRDTIDQG LYQDTIRLWR LFREILDGLA 840

YIHEKGMIHR DLKPVNIFLD SDDHVKIGDF GLATDHLAFT ADGKQDDTAD HLIKSDPSGH 900

LTGMVGTALY VSPEVQGSSR PTYNQKVDLF SLGIIFFEMS YHPMVTASER IFVLNQLRDP 960

TSPKFPEDFD DGEHTKQKSV ISWLLNHDPA KRPTATELLK SELLPPPQME ESELHEVLHH 1020

TLANVDGKAY RTMMAQIFSQ RVSPAIDYTY DSNMPKGFSI RTSKIQQHVC DTIVRIFKKH 1080

GAVQLCTPLL LPQNRQIYEH NEAALFMDHS GMLVMLPFDL RVPFARYVAR NNILNLKRYC 1140

IERVFRPRKL DRFHPKELLE CAFDIVTSTT NSSLPTAEVI YTICEIIQEF PALQERNYSI 1200

YLNHTMLLKA IFLHCGIPED KLSQVYVILY DAVTEKLTRR EVEAKFCNLS LSSNSLCRLY 1260

KFIEQKGDLQ DLTPAINSLI KQKTGVAQLV KYGLKDLEEI IGLLKKLGIK LQVLINLGLV 1320

YKVQQHNGVI FQFVAVIRRR QRAISEILAA GGRYDLLIPQ FRGPQALGPV PTAVGVSIAI 1380

DKISAAILNM EEPVTISSCD LLIVSVGQMS MSRAINLTQK LWSAGITAQI MYDWSQSQEE 1440

LQEYCRYHEI TYVALVSDKG GSYVKVKSFE KERQTEKLVQ ESDLIDHVLQ KLRTKVSDER 1500

TIREASDNLA VQNLKGSFSN ASGLFEIHGA AVVPIVSVIA PEKLSASTRR RYETQVQTRL 1560

QTSLANLHQK SNEIEILAVD LPKETILQFL SLEWDADEQA FNTTVKQLLS RLPKQRYLKL 1620

VCDEIYNIKV EKKVSVLFLY SYRDDYYRIL F 1651

Nucleotide Sequence
(FASTA)

ATGGCTGGGG GCCGTGGGGC CCCCGGGCGC GGCCGGGACG AGCCTCCGGA GAGCTACCCG 60

CAGCGGCAGG AGCACGAGCT GCAGGCCCTG GAGGCCATCT ACGGCGCCGA CTTCCAGGAC 120

CTGCGGCCGG ACGCGCGCAA ATCGGTCAAC GAGCCCCCTG AAATCAACTT AGTTCTGTAC 180

CCTCAGGGCC TAACTGGTGA AGAAGTATAC GTGCAAGTGG ATTTGAGGGT TAAATGCCCA 240

CCAACCTACC CAGATGTAGT CCCTGAAATA GAGTTAAAGA ATGCCAAAGG TCTATCAAAT 300

GAAAGTGTTC ATTTGTTAAA ATCCCACCTA GAGAAAGTGG CCAAAAAACA TTGTGGAGAG 360

GTGATGATAT TTGAACTGGC CGACCATGTG CAGTCATTTC TCAGCGAGCA TAACAAGCCC 420

CCTCCCAAGT CTTTCCATGA GGAAATGCTA GAAAGGCGGG CTCAGGAGGA GCAGCGGAGG 480

CTGTTGGAGG CCAAGCAGAA GGTAGAGCAG GAGCAACGTG AAATCCTTCA TGAGATTCAA 540

AGAAGGAAAG AAGAGATAAA AGAAGAGAGA AAAAGAAAAG AAATGGCTAA GCAGGAGCGT 600

TTGGAAATTG CTAGTTTGTC AAACCAAGAT CAAAACTCTA GGAAAGACCC AGGAGGACAC 660

AGAATATCTG CCATTCTACA TGGAGGCTCT CCTGACTTTG TAGGAAATGG TAAACATCGG 720

ACCAACTCCT CTGGAAGGTC TAGGCGAGAA CGTCAGTATT CTCTGTGTAG TAGTGAAGAT 780

TCTTCTGGCT CTTGTGAAGT CCTGTATTTC TCTATGGGTA GCCCTGATCA GCTCATGGTT 840

TACAAAGGGA GGTGCATTGG CAGTGATGAG CAGCTTGGAA AATTAGTTTA TAATGCTTTG 900

GAAACGGCCA CTGGCAACTT TGTGTCATTA CATGAGTGGG TCCTTCGGTG GCAGAAAAAA 960

ATGGGCCCGT TCCTTACCAG TCAAGAGAAG GAGAAGATTG ATAAGTGCAA AAAGCAGATT 1020

CAAGGGGCAG AAACGGAATT CAACTCACTG GTAAAATTGA GCCATCCAAA TGTAGTACGC 1080

TACTTTGCAA TGAATCTCAA AGAGCAAGAT GACTCCATCA TGGTGGACAT TTTATTGGAG 1140

CACATTAATG GGGTCTCCCT CTCTGCACAT CTGAGCCACT CAGGCCCCAT CCCTGTGTAT 1200

CAACTCCGCA AGTACACAGC TCAGCTCTTG TCTGGCCTCG ATTATTTGCA CAGCAATGAC 1260

GTGGTGCACA AGGTTCTGAA TGCATCAAAT GTCTTGGTGG ATGCAGAGGG CAATGTCAAG 1320

ATTACGGACT ACAGCATTTC AAAGCGCCTA GCAGACATTT GCAAGGAGGA TGTGTTTGAG 1380

CAGAGCCGAG TTAGTTTTAG TGACAGTGCC CTACCTTATA AAACGGGGAA GAAAGGGGAT 1440

GTTTGGTGTC TTGGCCTTCT GCTGCTCTTC CTCAGCCAAG GACAAGAATG CGGAGAGTAC 1500

CCTGTGACCA TCCCTAGCGA CTTACCAGCT GACTTCCAAG ATTTTCTAAA GAAGTGTGTT 1560

TGCTTGGATG ACAAGGAAAG ATGGAGTCCC CAGCAATTGT TGAAACACAG CTTTATCAAT 1620

CCACAGCCGA AAATGTCTTT ATTGGAACAG AGTCCTGACG ATTCTGGAGG ACAAGATTAT 1680

GTTGAGACTG TTATTCCTAG TAAGCAGCTA CCCAGTGCAG CATTCTTCAG TGAGACACAG 1740

AGACAGTTTT CCCGGTATTT CATTGAGTTT GAAGAATTAC AGCTTCTCGG CAAAGGAGCC 1800

TTCGGAGCTG TCATCAAGGT GCAGAACAAG CTGGATGGCT GCTGCTACGC GGTGAAGCGC 1860

ATCCCCATCA ATCCGGCCAG TAGGCATTTC CGCAGGATCA AGGGCGAGGT GACGCTGCTG 1920

TCGCGCCTGC ACCACGAGAA CATAGTGCGC TACTATAACG CCTGGATAGA GAGATACGAG 1980

CGGCCCGTGG GCCCGGGGAT GCTGCCGCAG GACTCGGACT CATGGCCCCA GGCACCGGAT 2040

GGGCGAGCTT TGTCCCAGCC GTCCCCAGGC AACGACAGCC CCGACGGCGA CGGCGCCGGT 2100

AGCGTGGAGG CCGCTGCGCC ACCGCCCATC CTCAGCTCGG TGGAGTGGAG CACATCTGGC 2160

GAGCGTTCGG CCAGCGCCCG CTTCCCCTCC GCCCGCCCAG GCTCCAGCAG CGAGGAGGAC 2220

GACGACGACG ACGAGCACGA GGGCGTCTTC TCCCAGCCCT TCCTACCTGC TTCAGATTCT 2280

GACAGTGATG TCATCTTTGA AGATGAGAAC AGTAAGAGTC AGAATCAGGA AGAAGACTGC 2340

AATGGGAAGA ACAGTTGCCA TGAAAGTGAG CCATCGACAA CCACTGAAGC TGTGCACTAC 2400

CTGTATATCC AGATGGAGTA CTGTGAGAAG AGTACTTTGC GTGACACCAT TGACCAGGGG 2460

CTATATCAAG ATACCATCAG ACTCTGGAGG CTTTTTCGAG AGATTCTGGA TGGATTAGCT 2520

TATATCCATG AAAAAGGAAT GATTCACCGG GATTTGAAGC CTGTCAACAT CTTTTTGGAT 2580

TCTGATGACC ATGTAAAAAT AGGTGATTTT GGTTTAGCGA CAGACCATCT TGCCTTCACT 2640

GCTGATGGCA AGCAAGATGA TACAGCAGAT CACTTGATTA AATCAGACCC CTCAGGTCAT 2700

TTGACTGGGA TGGTTGGTAC TGCTTTATAC GTAAGCCCAG AGGTCCAAGG AAGCAGCAGA 2760

CCCACGTACA ACCAGAAAGT GGATCTCTTC AGCCTGGGAA TTATCTTCTT TGAGATGTCC 2820

TATCACCCCA TGGTCACGGC CTCAGAAAGG ATCTTTGTTC TCAACCAACT CAGAGATCCC 2880

ACTTCACCTA AGTTTCCAGA AGACTTCGAT GATGGAGAGC ATACAAAGCA GAAATCTGTC 2940

ATCTCCTGGC TGTTGAACCA TGATCCCGCC AAGCGGCCCA CAGCCACAGA GCTGCTCAAG 3000

AGTGAGCTGC TGCCCCCACC CCAGATGGAG GAGTCAGAGT TGCATGAGGT TCTGCACCAC 3060

ACCCTGGCCA ACGTGGATGG GAAGGCCTAC CGCACCATGA TGGCCCAGAT CTTCTCCCAG 3120

CGCGTCAGTC CAGCCATCGA TTACACCTAT GACAGCAACA TGCCGAAGGG CTTTTCAATC 3180

CGTACATCCA AGATACAGCA ACATGTGTGT GATACCATCG TCCGCATTTT TAAAAAACAC 3240

GGAGCTGTCC AGTTGTGTAC TCCACTACTG CTTCCCCAAA ATAGGCAAAT ATACGAGCAC 3300

AATGAAGCTG CCTTATTCAT GGACCACAGC GGGATGCTGG TGATGCTTCC CTTTGACCTA 3360

CGGGTTCCTT TTGCAAGATA TGTGGCAAGA AATAATATAT TGAATTTGAA ACGTTACTGC 3420

ATAGAACGTG TCTTCAGACC TCGCAAGTTA GACCGATTTC ATCCCAAAGA ACTTCTGGAA 3480

TGTGCATTTG ACATTGTTAC GTCTACCACC AACAGCTCTC TGCCCACTGC TGAAGTCATC 3540

TACACGATCT GTGAGATCAT CCAGGAGTTC CCGGCACTGC AGGAAAGAAA TTACAGTATT 3600

TACTTGAACC ACACCATGTT ATTGAAAGCA ATCTTCTTAC ACTGTGGGAT CCCAGAAGAT 3660

AAACTCAGTC AAGTCTATGT TATTCTATAT GATGCAGTGA CAGAAAAGCT GACAAGGAGA 3720

GAAGTGGAAG CCAAGTTTTG TAATCTGTCT CTGTCATCTA ATAGTCTGTG TCGACTCTAC 3780

AAATTCATTG AGCAGAAGGG AGATTTGCAA GATCTAACAC CAGCAATAAA TTCATTAATA 3840

AAACAGAAAA CAGGTGTTGC CCAACTGGTG AAGTATGGCT TGAAAGACTT AGAGGAGATC 3900

ATTGGGCTGT TGAAGAAACT CGGCATCAAG TTACAGGTCT TGATCAACTT GGGCTTGGTT 3960

TACAAAGTAC AGCAGCACAA CGGAGTCATC TTCCAGTTTG TGGCGGTCAT CAGACGGAGG 4020

CAAAGGGCTA TTTCAGAAAT CCTTGCAGCT GGAGGCAGAT ACGACCTGCT GATTCCCCAG 4080

TTTAGAGGAC CGCAGGCTCT GGGACCAGTC CCGACTGCTG TTGGGGTCAG CATAGCCATA 4140

GACAAGATAT CTGCAGCTAT CCTCAACATG GAAGAACCTG TTACAATAAG CTCTTGTGAC 4200

CTCCTGATCG TAAGTGTTGG CCAGATGTCC ATGTCCAGGG CCATCAACCT AACCCAGAAA 4260

CTTTGGTCAG CAGGAATCAC AGCACAGATC ATGTACGACT GGTCACAGTC CCAAGAAGAA 4320

CTCCAGGAAT ACTGCAGATA CCACGAAATC ACCTACGTGG CCCTCGTCTC AGATAAAGGA 4380

GGAAGCTATG TCAAGGTTAA GTCTTTTGAG AAGGAAAGGC AAACAGAGAA ACTTGTGCAG 4440

GAGTCTGATC TTATAGACCA CGTTTTGCAG AAACTCAGAA CTAAAGTCAG TGATGAAAGG 4500

ACTATCAGAG AAGCTTCTGA TAATCTCGCA GTGCAAAATC TGAAGGGGTC CTTTTCTAAT 4560

GCTTCAGGTT TGTTTGAAAT CCACGGAGCA GCAGTTGTCC CCATTGTGAG CGTGATAGCC 4620

CCAGAGAAGC TGTCAGCCAG CACGAGGAGG CGTTATGAAA CTCAGGTACA AACACGACTT 4680

CAGACCTCCC TTGCCAACTT ACATCAGAAA AGCAATGAAA TTGAAATTTT GGCTGTGGAT 4740

CTACCCAAAG AAACAATCTT ACAGTTTTTA TCGTTAGAGT GGGATGCTGA TGAACAGGCA 4800

TTTAACACAA CTGTGAAGCA GCTGCTGTCA CGCCTGCCAA AGCAGAGATA CCTCAAATTA 4860

GTCTGTGATG AAATTTATAA CATCAAAGTA GAGAAAAAGG TATCTGTGCT ATTCCTGTAT 4920

AGCTACAGAG ATGACTACTA CCGAATCTTA TTTTAA 4956

Domain Profile

S: 1     feeikvlGkGgfgvVlkaknkldkkeyaikriklnkakkelekverevkaLaklehpniv  60

         fee+++lGkG+fg+V+k++nkld+++ya+kri++n a++++++++ ev++L++l+h+niv

Q: 590   FEELQLLGKGAFGAVIKVQNKLDGCCYAVKRIPINPASRHFRRIKGEVTLLSRLHHENIV  649

         89**********************************************************

S: 61    rYfaawleekeesl..............................................  74

         rY++aw+e+ e+++

Q: 650   RYYNAWIERYERPVgpgmlpqdsdswpqapdgralsqpspgndspdgdgagsveaaappp  709

         ************************************************************

S: 75    ............................................................  74

Q: 710   ilssvewstsgersasarfpsarpgssseedddddehegvfsqpflpasdsdsdvifede  769

         ********9999999999999999999999999999999999999999875555555444

S: 75    .tdsseveaeaeleesesseesdtk................llyiqmelceketLkdwid  117

          +           ++++++e+ + k                +lyiqme+cek+tL+d+id

Q: 770   nS-----------KSQNQEEDCNGKnschesepsttteavhYLYIQMEYCEKSTLRDTID  818

         31...........3333333333335555555566677888*******************

S: 118   erkllesrselvmrlfkqllegleylHskgliHrdLkpsniFldsddqvKigDFgLatal  177

         +  l++++ +l+ rlf+++l+gl+y+H+kg+iHrdLkp+niFldsdd+vKigDFgLat+

Q: 819   QG-LYQDTIRLW-RLFREILDGLAYIHEKGMIHRDLKPVNIFLDSDDHVKIGDFGLATD-  875

         **.9********.*********************************************9.

S: 178   aealkedvkqdaqske.......akhtgqvgtklymsPeql..eksqYnkkvDifsLGli  228

         + a+++d kqd+++ +       ++ tg+vgt+ly+sPe++  ++ +Yn+kvD+fsLG+i

Q: 876   HLAFTADGKQDDTADHliksdpsGHLTGMVGTALYVSPEVQgsSRPTYNQKVDLFSLGII  935

         888888999988888788888877*****************888889*************

S: 229   lfeLly.pfstemervetltelrd...gkipedfdd.kvpkekkllqkllsrdpkeRpsa  283

         +fe++y p+ t++er+ +l++lrd    k+pedfdd +++k+k+++++ll++dp++Rp+a

Q: 936   FFEMSYhPMVTASERIFVLNQLRDptsPKFPEDFDDgEHTKQKSVISWLLNHDPAKRPTA  995

         ************************9999********************************

S: 284   sellksell  292

         +ellksell

Q: 996   TELLKSELL  1004

         ******986

Domain Sequence
(FASTA)

FEELQLLGKG AFGAVIKVQN KLDGCCYAVK RIPINPASRH FRRIKGEVTL LSRLHHENIV 60

RYYNAWIERY ERPVGPGMLP QDSDSWPQAP DGRALSQPSP GNDSPDGDGA GSVEAAAPPP 120

ILSSVEWSTS GERSASARFP SARPGSSSEE DDDDDEHEGV FSQPFLPASD SDSDVIFEDE 180

NSKSQNQEED CNGKNSCHES EPSTTTEAVH YLYIQMEYCE KSTLRDTIDQ GLYQDTIRLW 240

RLFREILDGL AYIHEKGMIH RDLKPVNIFL DSDDHVKIGD FGLATDHLAF TADGKQDDTA 300

DHLIKSDPSG HLTGMVGTAL YVSPEVQGSS RPTYNQKVDL FSLGIIFFEM SYHPMVTASE 360

RIFVLNQLRD PTSPKFPEDF DDGEHTKQKS VISWLLNHDP AKRPTATELL KSELL 415

Keyword

ATP-binding; Complete proteome; Nucleotide-binding; Reference proteome.

Sequence Source

Ensembl

Orthology

Ortholog group

Anolis carolinensis"; ?>Bos taurus"; ?>Caenorhabditis elegans"; ?>Callithrix jacchus"; ?>Cavia porcellus"; ?>Ciona intestinalis"; ?>Danio rerio"; ?>Drosophila melanogaster"; ?>Equus caballus"; ?>Felis catus"; ?>Gallus gallus"; ?>Gasterosteus aculeatus"; ?>Gorilla gorilla"; ?>Homo sapiens"; ?>Ictidomys tridecemlineatus"; ?>Latimeria chalumnae"; ?>Loxodonta africana"; ?>Meleagris gallopavo"; ?>Monodelphis domestica"; ?>Mus musculus"; ?>Mustela putorius furo"; ?>Myotis lucifugus"; ?>Nomascus leucogenys"; ?>Oreochromis niloticus"; ?>Ornithorhynchus anatinus"; ?>Oryctolagus cuniculus"; ?>Oryzias latipes"; ?>Otolemur garnettii"; ?>Pan troglodytes"; ?>Pelodiscus sinensis"; ?>Petromyzon marinus"; ?>Pongo abelii"; ?>Rattus norvegicus"; ?>Sarcophilus harrisii"; ?>Sus scrofa"; ?>Taeniopygia guttata"; ?>Takifugu rubripes"; ?>Tetraodon nigroviridis"; ?>Tursiops truncatus"; ?>Xenopus tropicalis"; ?>Xiphophorus maculatus"; ?>Saccharomyces cerevisiae"; ?>Schizosaccharomyces pombe"; ?>

Gene Ontology

GO:0005524	; F:ATP binding
GO:0004694	; F:eukaryotic translation initiation factor 2alpha kinase activity
GO:0000049	; F:tRNA binding
GO:0009267	; P:cellular response to starvation
GO:0030968	; P:endoplasmic reticulum unfolded protein response
GO:0017148	; P:negative regulation of translation
GO:0043558	; P:regulation of translational initiation in response to stress

KEGG

cfa:478257

;

InterPros

IPR026920	; EIF2AK4.
IPR024435	; GCN2_anticodon-bd_dom.
IPR011009	; Kinase-like_dom.
IPR000719	; Prot_kinase_cat_dom.
IPR017441	; Protein_kinase_ATP_BS.
IPR006575	; RWD-domain.
IPR008271	; Ser/Thr_kinase_AS.
IPR016135	; UBQ-conjugating_enzyme/RWD.

Pfam

PF12745	; HGTP_anticodon2; 1.
PF00069	; Pkinase; 3.
PF05773	; RWD; 1.

SMARTs

SM00591

; RWD; 1.

Prosites

PS00107	; PROTEIN_KINASE_ATP; 1.
PS50011	; PROTEIN_KINASE_DOM; 2.
PS00108	; PROTEIN_KINASE_ST; 1.
PS50908	; RWD; 1.

Prints

Created Date

20-Feb-2013