EKS-CAF-00403

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EKS-CAF-00403

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
TK/PDGFR	597.0	1.6E-179	631	989	359

Status

Reviewed

Ensembl Protein

ENSCAFP00000000714

UniProt Accession

Q6QNF3;

Protein Name

Platelet-derived growth factor receptor beta

Protein Synonyms/Alias

PDGF-R-beta; PDGFR-beta; Beta platelet-derived growth factor receptor; Beta-type platelet-derived growth factor receptor; CD140 antigen-like family member B; Platelet-derived growth factor receptor 1; PDGFR-1;

Gene Name

PDGFRB

Gene Synonyms/Alias

PDGFRB; PDGFR, PDGFR1;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
ENSCAFG00000018214	ENSCAFP00000000714	ENSCAFT00000000773

Organism

Canis familiaris

Functional Description

Tyrosine-protein kinase that acts as cell-surfacereceptor for homodimeric PDGFB and PDGFD and for heterodimers formed by PDGFA and PDGFB, and plays an essential role in the regulation of embryonic development, cell proliferation, survival, differentiation, chemotaxis and migration. Plays an essential role in blood vessel development by promoting proliferation, migration and recruitment of pericytes and smooth muscle cells to endothelial cells. Plays a role in the migration of vascular smooth muscle cells and the formation of neointima at vascular injury sites. Required for normal development of the cardiovascular system. Required for normal recruitment of pericytes (mesangial cells) in the kidney glomerulus, and for normal formation of a branched network of capillaries in kidney glomeruli. Promotes rearrangement of the actin cytoskeleton and the formation of membrane ruffles. Binding of its cognate ligands - homodimeric PDGFB, heterodimers formed by PDGFA and PDGFB or homodimeric PDGFD -leads to the activation of several signaling cascades; the response depends on the nature of the bound ligand and is modulated by the formation of heterodimers between PDGFRA and PDGFRB. Phosphorylates PLCG1, PIK3R1, PTPN11, RASA1/GAP, CBL, SHC1 and NCK1. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5- trisphosphate, mobilization of cytosolic Ca(2+) and the activation of protein kinase C. Phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, leads to the activation of the AKT1 signaling pathway. Phosphorylation of SHC1, or of the C-terminus of PTPN11, creates a binding site for GRB2, resulting in the activation of HRAS, RAF1 and down-stream MAP kinases, including MAPK1/ERK2 and/or MAPK3/ERK1. Promotes phosphorylation and activation of SRC family kinases. Promotes phosphorylation of PDCD6IP/ALIX and STAM. Receptor signaling is down-regulated by protein phosphatases that dephosphorylate the receptor and its down-stream effectors, and by rapid internalization of the activated receptor (By similarity).

Protein Length

1134

Protein Sequence
(FASTA)

MRGQRGGLPG GGDSPELRTV PTPEGISSKD TMQVPGTMPA PVLKGQALWL PLLLMLSPQA 60

SGGLVITPPG PELVLNISST FVLTCSGPAP VVWERLSQEP LQKMARTQDG TFSSTLTLTN 120

VTGLDTGEYF CTYKGSHGLE PDGRKRLYIF VPDPTMGFLP VDPEELFIFL TEITEITIPC 180

RVTDPRLVVT LHEKKVDIPL PIPYDHQRGF SGTFEDKTYV CKTTIGDKEV DSEAYYVYSL 240

QVSSINVSVN AVQTVVRQGE NITIMCIVTG NEVVNFEWTY PRMESGRLVE PVTDFLFNVP 300

SHIRSILHIP SAELGDSGTY ICNVSESVND HRDEKSINVT VVESGYVRLL GELDAVQFAE 360

LHRSRALQVV FEAYPPPTVV WFKDNRTLGD SSAGEIALST RNVSETRYVS ELTLVRVKVA 420

EAGYYTMRAF HEDAEAQLSF QLQVNVPVRV LELSESHPAS GEQTVRCRGR GMPQPHLTWS 480

TCSDLKRCPR ELPPTPLGNS SEEESQLETN VTYWPEDQEF EVVSTLRLRR VDQPLSVRCT 540

LHNLLGHDMQ EVTVVPHSLP FKVVVISAIL ALVVLTIISL IILIMLWQKK PRYEIRWKVI 600

ESVSSDGHEY IYVDPMQLPY DSTWELPRDQ LVLGRTLGSG AFGQVVEATA HGLSHSQATM 660

KVAVKMLKST ARSSEKQALM SELKIMSHLG PHLNVVNLLG ACTKGGPIYI ITEYCRYGDL 720

VDYLHRNKHT FLQLCSDKRR PPSAELYSNA LPAGLPLPSH VSLPGESDGG YMDMSKDESV 780

DYVPMLDMKG GVKYADIESS SYMAPYDNYV PTAPERTCRA TLINESPVLS YTDLVGFSYQ 840

VANGMEFLAS KNCVHRDLAA RNVLICEGKL VKICDFGLAR DIMRDSNYIS KGSTFLPLKW 900

MAPESIFNSL YTTLSDVWSF GILLWEIFTL GGTPYPELPM NEQFYNAIKR GYRMAQPAHA 960

SDEIYEIMQK CWEEKFEIRP PFSQLVLLLE RLLGEGYKKK YQQVDEEFLR SDHPAVLRSQ 1020

ARLPGFPGLR SPLDTSSVLY TAVQPNEGDN DYIIPLPDPK PEVADGPLES SPSLASSTLN 1080

EVNTSSTISC DSPLEPQEEP EPEPEPQPEP QVVPEPPLDS SCPGPRAEAE DSFL 1134

Nucleotide Sequence
(FASTA)

ATGAGAGGTC AGCGAGGAGG ACTTCCTGGA GGGGGTGACA GCCCAGAGCT CAGGACTGTG 60

CCCACCCCCG AAGGCATCAG CAGCAAGGAC ACCATGCAGG TTCCAGGCAC CATGCCAGCT 120

CCTGTCCTCA AAGGCCAGGC ACTGTGGCTG CCGCTGCTGT TGATGCTGAG CCCACAGGCC 180

TCCGGTGGCC TGGTCATCAC ACCCCCGGGG CCGGAGCTTG TCCTTAATAT CTCTAGCACC 240

TTCGTTCTGA CCTGCTCGGG TCCAGCTCCG GTGGTATGGG AACGGTTGTC CCAAGAGCCC 300

CTGCAGAAAA TGGCCAGGAC CCAGGATGGC ACCTTCTCCA GCACACTGAC ACTGACCAAT 360

GTCACTGGGC TAGACACGGG AGAGTACTTT TGCACCTACA AAGGCTCCCA TGGGCTGGAG 420

CCTGATGGGC GAAAACGGCT CTATATCTTT GTGCCAGATC CCACTATGGG CTTTCTCCCG 480

GTTGACCCTG AGGAACTCTT CATCTTTCTC ACGGAAATAA CGGAGATCAC AATTCCATGC 540

CGAGTGACGG ATCCAAGACT GGTGGTGACA CTACACGAGA AGAAAGTGGA TATCCCACTG 600

CCCATCCCCT ATGACCACCA ACGTGGCTTC TCTGGTACCT TCGAGGACAA GACTTATGTC 660

TGCAAGACCA CCATCGGGGA CAAGGAAGTG GATTCTGAAG CCTACTATGT CTACAGCCTC 720

CAGGTGTCAT CCATCAACGT CTCCGTGAAT GCAGTGCAGA CTGTGGTCCG CCAGGGTGAG 780

AACATCACCA TCATGTGCAT CGTGACCGGA AATGAGGTGG TTAACTTTGA ATGGACATAC 840

CCACGCATGG AGAGTGGGCG GCTGGTGGAG CCAGTGACTG ACTTCCTCTT CAATGTGCCC 900

TCCCACATAC GCTCCATTCT GCACATCCCT AGTGCTGAGC TTGGCGATTC AGGGACCTAC 960

ATCTGCAATG TATCAGAAAG TGTGAATGAC CATCGAGATG AAAAGTCCAT CAACGTCACC 1020

GTGGTTGAGA GCGGCTACGT GCGGCTGCTG GGGGAGCTGG ACGCCGTACA GTTCGCGGAG 1080

CTGCACCGCA GCCGGGCTCT GCAGGTCGTG TTCGAGGCCT ACCCGCCGCC TACTGTCGTG 1140

TGGTTCAAGG ACAACCGCAC GCTAGGCGAC TCCAGCGCAG GCGAGATCGC CTTGTCCACA 1200

CGCAACGTGT CTGAGACCCG GTACGTGTCA GAACTGACGT TGGTGCGGGT CAAGGTGGCC 1260

GAGGCTGGCT ACTACACCAT GCGGGCCTTC CATGAGGATG CCGAGGCCCA GCTCTCCTTC 1320

CAGCTGCAGG TCAACGTGCC TGTCCGCGTG CTGGAGCTGA GTGAAAGCCA CCCTGCCAGC 1380

GGGGAGCAGA CAGTCCGATG TCGCGGCCGG GGCATGCCCC AGCCACACCT CACCTGGTCT 1440

ACCTGCAGCG ACCTCAAAAG ATGTCCTCGC GAGCTGCCAC CCACGCCGCT AGGGAACAGT 1500

TCAGAGGAGG AGAGCCAGCT GGAGACTAAC GTGACATACT GGCCTGAGGA TCAGGAGTTC 1560

GAGGTGGTGA GCACGCTGCG CCTGCGCCGT GTGGATCAGC CGCTGTCGGT GCGCTGCACT 1620

CTGCACAACC TGCTGGGACA CGACATGCAG GAGGTCACCG TGGTGCCACA TTCTCTGCCC 1680

TTCAAGGTGG TGGTGATCTC GGCCATCCTG GCCTTGGTGG TTCTCACAAT CATCTCCCTC 1740

ATCATCCTCA TCATGCTCTG GCAAAAGAAG CCACGTTATG AGATCCGATG GAAGGTGATT 1800

GAATCCGTGA GCTCCGATGG CCATGAGTAC ATCTACGTGG ACCCTATGCA GCTGCCCTAC 1860

GACTCCACCT GGGAGCTGCC GAGGGACCAG CTCGTGCTTG GACGTACGCT CGGCTCTGGG 1920

GCCTTTGGGC AGGTGGTGGA GGCGACGGCT CACGGCCTAA GCCATTCGCA GGCCACGATG 1980

AAAGTGGCAG TCAAGATGCT GAAATCCACA GCCCGCAGCA GCGAGAAGCA GGCCCTCATG 2040

TCAGAGCTGA AGATCATGAG TCACCTCGGG CCCCATCTAA ATGTGGTCAA CTTGCTGGGG 2100

GCCTGCACCA AAGGAGGACC CATCTACATC ATCACCGAGT ACTGCCGCTA CGGGGACCTG 2160

GTGGACTACC TTCACCGCAA CAAGCACACC TTCCTGCAGC TCTGCTCCGA CAAGCGCCGC 2220

CCGCCCAGCG CCGAGCTCTA CAGCAACGCC CTGCCCGCGG GGCTCCCTCT GCCCAGCCAC 2280

GTGTCTCTGC CTGGGGAGAG TGACGGGGGC TACATGGACA TGAGCAAGGA CGAGTCAGTG 2340

GATTACGTGC CCATGCTGGA CATGAAAGGA GGCGTCAAAT ATGCAGACAT TGAGTCCTCC 2400

AGCTACATGG CCCCTTATGA CAACTACGTC CCCACTGCTC CCGAGAGGAC CTGTCGGGCA 2460

ACTCTGATCA ATGAGTCCCC AGTGCTTAGC TACACGGATC TCGTGGGCTT CAGCTACCAG 2520

GTGGCCAATG GCATGGAGTT CCTGGCCTCC AAGAACTGTG TCCACCGAGA TCTGGCAGCC 2580

AGAAACGTGC TCATCTGTGA GGGCAAGCTG GTCAAGATAT GTGACTTTGG CCTGGCTCGT 2640

GACATCATGA GAGACTCGAA CTATATCTCC AAAGGCAGCA CCTTCCTGCC TCTGAAGTGG 2700

ATGGCTCCCG AGAGCATCTT CAACAGCCTC TATACCACCC TGAGCGACGT GTGGTCCTTC 2760

GGGATCCTGC TCTGGGAGAT CTTCACCCTG GGTGGCACAC CTTACCCAGA GCTGCCCATG 2820

AACGAACAGT TCTATAATGC CATCAAGCGG GGCTACCGCA TGGCTCAGCC TGCCCACGCC 2880

TCTGACGAGA TTTATGAGAT CATGCAGAAG TGCTGGGAAG AGAAGTTTGA GATCCGGCCT 2940

CCCTTCTCCC AGCTGGTGCT GCTTCTCGAG AGGCTGCTGG GTGAGGGGTA CAAAAAGAAG 3000

TACCAGCAGG TGGATGAGGA GTTTCTGAGG AGCGACCACC CAGCAGTCCT TCGGTCCCAA 3060

GCTCGCTTGC CTGGCTTCCC TGGCCTCCGA TCCCCTCTGG ACACCAGCTC TGTCCTGTAC 3120

ACTGCTGTGC AGCCCAACGA GGGTGACAAC GACTACATCA TCCCCCTGCC GGACCCCAAG 3180

CCTGAGGTTG CTGACGGCCC ACTGGAGAGT TCTCCCAGCC TTGCCAGCTC CACCCTGAAT 3240

GAAGTCAACA CCTCCTCTAC CATCTCCTGT GACAGCCCCC TGGAACCCCA AGAGGAGCCA 3300

GAACCAGAGC CAGAGCCCCA GCCCGAGCCC CAGGTGGTGC CGGAGCCACC GCTGGATTCA 3360

AGCTGCCCTG GGCCTCGGGC TGAGGCAGAG GACAGCTTCC TGTAG 3405

Domain Profile

S: 1     lelGkvlGsGafGkvveatayGlsksdavlkvavkmlkstarssekealmselkilshlG  60

         l+lG++lGsGafG+vveata+Gls+s+a++kvavkmlkstarssek+almselki+shlG

Q: 631   LVLGRTLGSGAFGQVVEATAHGLSHSQATMKVAVKMLKSTARSSEKQALMSELKIMSHLG  690

         78**********************************************************

S: 61    ehenivnllGactkgGpiyviteyccyGdllnylrrkresflkkslekkkekeeeldska  120

         +h+n+vnllGactkgGpiy+iteyc+yGdl++yl+r++++fl+  ++k++++++el+s+a

Q: 691   PHLNVVNLLGACTKGGPIYIITEYCRYGDLVDYLHRNKHTFLQLCSDKRRPPSAELYSNA  750

         ************************************************************

S: 121   lladkslrsksslssesseeyvdlkkd................vsdvssskadklasekk  164

         l+a+  l s++sl  es++ y+d++kd                ++d++ss++++++++++

Q: 751   LPAGLPLPSHVSLPGESDGGYMDMSKDesvdyvpmldmkggvkYADIESSSYMAPYDNYV  810

         ************************************************************

S: 165   ksalerevkaaleeedsevleledllsfsyqvakGmeflaskncvhrdlaarnvllteGk  224

           a er+++a+l++e s vl+++dl+ fsyqva+Gmeflaskncvhrdlaarnvl++eGk

Q: 811   PTAPERTCRATLINE-SPVLSYTDLVGFSYQVANGMEFLASKNCVHRDLAARNVLICEGK  869

         **************9.9*******************************************

S: 225   vvkicdfGlardimkdsnyvvkGnarlpvkwmapesifdslytvksdvwsyGillweifs  284

         +vkicdfGlardim+dsny++kG+++lp+kwmapesif+slyt+ sdvws+Gillweif+

Q: 870   LVKICDFGLARDIMRDSNYISKGSTFLPLKWMAPESIFNSLYTTLSDVWSFGILLWEIFT  929

         ************************************************************

S: 285   lGstpypgllvdskfyklikeGyrmaqpayateeiyeimqkcweaepekrpsfkqlvell  344

         lG+tpyp+l+++++fy++ik+Gyrmaqpa+a++eiyeimqkcwe+++e rp+f+qlv ll

Q: 930   LGGTPYPELPMNEQFYNAIKRGYRMAQPAHASDEIYEIMQKCWEEKFEIRPPFSQLVLLL  989

         ********************************************************9986

Domain Sequence
(FASTA)

LVLGRTLGSG AFGQVVEATA HGLSHSQATM KVAVKMLKST ARSSEKQALM SELKIMSHLG 60

PHLNVVNLLG ACTKGGPIYI ITEYCRYGDL VDYLHRNKHT FLQLCSDKRR PPSAELYSNA 120

LPAGLPLPSH VSLPGESDGG YMDMSKDESV DYVPMLDMKG GVKYADIESS SYMAPYDNYV 180

PTAPERTCRA TLINESPVLS YTDLVGFSYQ VANGMEFLAS KNCVHRDLAA RNVLICEGKL 240

VKICDFGLAR DIMRDSNYIS KGSTFLPLKW MAPESIFNSL YTTLSDVWSF GILLWEIFTL 300

GGTPYPELPM NEQFYNAIKR GYRMAQPAHA SDEIYEIMQK CWEEKFEIRP PFSQLVLLL 359

Keyword

ATP-binding; Cell membrane; Chemotaxis; Complete proteome; Cytoplasmic vesicle; Developmental protein; Disulfide bond; Glycoprotein; Immunoglobulin domain; Kinase; Lysosome; Membrane; Nucleotide-binding; Phosphoprotein; Receptor; Reference proteome; Repeat; Signal; Transferase; Transmembrane; Transmembrane helix; Tyrosine-protein kinase; Ubl conjugation.

Sequence Source

Ensembl

Orthology

Ortholog group

Ailuropoda melanoleuca"; ?>Anolis carolinensis"; ?>Bos taurus"; ?>Callithrix jacchus"; ?>Cavia porcellus"; ?>Danio rerio"; ?>Equus caballus"; ?>Felis catus"; ?>Gallus gallus"; ?>Gasterosteus aculeatus"; ?>Gorilla gorilla"; ?>Homo sapiens"; ?>Ictidomys tridecemlineatus"; ?>Latimeria chalumnae"; ?>Loxodonta africana"; ?>Macaca mulatta"; ?>Meleagris gallopavo"; ?>Monodelphis domestica"; ?>Mus musculus"; ?>Mustela putorius furo"; ?>Myotis lucifugus"; ?>Nomascus leucogenys"; ?>Ochotona princeps"; ?>Oreochromis niloticus"; ?>Ornithorhynchus anatinus"; ?>Oryctolagus cuniculus"; ?>Oryzias latipes"; ?>Otolemur garnettii"; ?>Pelodiscus sinensis"; ?>Pongo abelii"; ?>Rattus norvegicus"; ?>Sarcophilus harrisii"; ?>Sus scrofa"; ?>Taeniopygia guttata"; ?>Takifugu rubripes"; ?>Tetraodon nigroviridis"; ?>Xenopus tropicalis"; ?>Xiphophorus maculatus"; ?>

Gene Ontology

GO:0016324	; C:apical plasma membrane
GO:0005737	; C:cytoplasm
GO:0016023	; C:cytoplasmic membrane-bounded vesicle
GO:0005887	; C:integral to plasma membrane
GO:0031226	; C:intrinsic to plasma membrane
GO:0043202	; C:lysosomal lumen
GO:0005634	; C:nucleus
GO:0005524	; F:ATP binding
GO:0005019	; F:platelet-derived growth factor beta-receptor activity
GO:0005021	; F:vascular endothelial growth factor-activated receptor activity
GO:0030325	; P:adrenal gland development
GO:0060326	; P:cell chemotaxis
GO:0060981	; P:cell migration involved in coronary angiogenesis
GO:0035441	; P:cell migration involved in vasculogenesis
GO:0001701	; P:in utero embryonic development
GO:0001822	; P:kidney development
GO:0006807	; P:nitrogen compound metabolic process
GO:0018108	; P:peptidyl-tyrosine phosphorylation
GO:0046488	; P:phosphatidylinositol metabolic process
GO:0048015	; P:phosphatidylinositol-mediated signaling
GO:0038091	; P:positive regulation of cell proliferation by VEGF-activated platelet derived growth factor receptor signaling pathway
GO:0070374	; P:positive regulation of ERK1 and ERK2 cascade
GO:0043552	; P:positive regulation of phosphatidylinositol 3-kinase activity
GO:0010863	; P:positive regulation of phospholipase C activity
GO:0032516	; P:positive regulation of phosphoprotein phosphatase activity
GO:0014911	; P:positive regulation of smooth muscle cell migration
GO:0048661	; P:positive regulation of smooth muscle cell proliferation
GO:0046777	; P:protein autophosphorylation
GO:0050730	; P:regulation of peptidyl-tyrosine phosphorylation
GO:0061298	; P:retina vasculature development in camera-type eye
GO:0048705	; P:skeletal system morphogenesis
GO:0048745	; P:smooth muscle tissue development
GO:0001894	; P:tissue homeostasis
GO:0048010	; P:vascular endothelial growth factor receptor signaling pathway

KEGG

cfa:442985

;

InterPros

IPR007110	; Ig-like.
IPR013783	; Ig-like_fold.
IPR013098	; Ig_I-set.
IPR003599	; Ig_sub.
IPR003598	; Ig_sub2.
IPR011009	; Kinase-like_dom.
IPR000719	; Prot_kinase_cat_dom.
IPR017441	; Protein_kinase_ATP_BS.
IPR001245	; Ser-Thr/Tyr_kinase_cat_dom.
IPR008266	; Tyr_kinase_AS.
IPR020635	; Tyr_kinase_cat_dom.
IPR016243	; Tyr_kinase_CSF1/PDGF_rcpt.
IPR001824	; Tyr_kinase_rcpt_3_CS.
IPR009134	; Tyr_kinase_VEGFR_rcpt_N.

Pfam

PF07679	; I-set; 1.
PF07714	; Pkinase_Tyr; 1.

SMARTs

SM00409	; IG; 1.
SM00408	; IGc2; 2.
SM00219	; TyrKc; 1.

Prosites

PS50835	; IG_LIKE; 2.
PS00107	; PROTEIN_KINASE_ATP; 1.
PS50011	; PROTEIN_KINASE_DOM; 1.
PS00109	; PROTEIN_KINASE_TYR; 1.
PS00240	; RECEPTOR_TYR_KIN_III; 1.

Prints

PR01832

; VEGFRECEPTOR.

Created Date

20-Feb-2013