EKS-CAF-00235

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EKS-CAF-00235

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
STE/STE7	382.6	1.2E-114	73	369	297

Status

Reviewed

Ensembl Protein

ENSCAFP00000028245

UniProt Accession

Q1HG70;

Protein Name

Dual specificity mitogen-activated protein kinase kinase 2

Protein Synonyms/Alias

MAP kinase kinase 2; MAPKK 2; ERK activator kinase 2; MAPK/ERK kinase 2; MEK 2;

Gene Name

MAP2K2

Gene Synonyms/Alias

MAP2K2; MEK2;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
ENSCAFG00000019138	ENSCAFP00000028245	ENSCAFT00000030400

Organism

Canis familiaris

Functional Description

Catalyzes the concomitant phosphorylation of a threonineand a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates the ERK1 and ERK2 MAP kinases (By similarity).

Protein Length

400

Protein Sequence
(FASTA)

MLARRKPVLP ALTINPAIAE GPSPTSEGAS EANLVDLQKK LAELELDEQQ KKRLEAFLTQ 60

KAKVGELKDD DFERISELGA GNGGVVTKVQ HRPSGLIMAR KLIHLEIKPA IRNQIIRELQ 120

VLHECNSPYI VGFYGAFYSD GEISICMEHM DGGSLDQVLK EAKRIPEEIL GKVSIAVLRG 180

LAYLREKHQI MHRDVKPSNI LVNSRGEIKL CDFGVSGQLI DSMANSFVGT RSYMSPERLQ 240

GTHYSVQSDI WSMGLSLVEL SIGRYPIPPP DAKELEAIFG RPMVDGIEGE PHSISPRPRP 300

PGRPISGHGT DSRPAMAIFE LLDYIVNEPP PKLPNGVFTQ DFQEFVNKCL IKNPAERADL 360

KMLMSHTFIK RSEVEEVDFA GWLCKTLRLN QPSTPTRTAV 400

Nucleotide Sequence
(FASTA)

ATGCTGGCCC GGAGGAAGCC GGTGCTGCCG GCGCTCACTA TCAACCCCGC CATCGCCGAG 60

GGCCCGTCCC CCACCAGCGA GGGCGCCTCC GAAGCAAACT TGGTGGACCT CCAGAAGAAG 120

CTAGCAGAGC TAGAACTTGA TGAGCAGCAG AAGAAGCGAC TGGAAGCCTT CCTCACCCAG 180

AAGGCCAAGG TCGGGGAACT CAAGGATGAC GACTTCGAAC GGATCTCAGA GCTGGGAGCC 240

GGGAACGGCG GGGTGGTCAC CAAGGTCCAG CACAGACCTT CTGGCCTCAT CATGGCGAGA 300

AAGCTGATCC ACCTGGAAAT CAAGCCGGCC ATCCGGAACC AGATCATCCG CGAGCTGCAG 360

GTGCTGCACG AGTGTAACTC CCCATACATC GTGGGCTTCT ACGGGGCCTT CTACAGCGAC 420

GGCGAGATCA GCATCTGCAT GGAGCATATG GATGGCGGCT CTCTGGACCA GGTGTTGAAG 480

GAAGCCAAGA GAATCCCTGA GGAGATCCTG GGGAAGGTCA GCATTGCGGT TCTCCGGGGT 540

TTGGCGTATC TCCGGGAGAA GCACCAGATC ATGCACCGAG ACGTGAAACC ATCCAACATC 600

CTCGTCAACT CCCGAGGGGA GATTAAGCTG TGTGATTTTG GGGTGAGCGG CCAGCTCATC 660

GACTCCATGG CCAACTCCTT CGTGGGAACT CGGTCCTACA TGTCTCCAGA GCGGTTGCAG 720

GGCACTCATT ACTCAGTGCA GTCGGACATC TGGAGCATGG GCCTGTCCCT GGTGGAGCTG 780

TCCATTGGAA GGTACCCCAT CCCGCCACCA GATGCCAAGG AGCTAGAGGC CATCTTCGGC 840

CGGCCCATGG TTGACGGGAT AGAAGGAGAG CCTCACAGCA TCTCACCACG GCCGAGACCC 900

CCTGGACGCC CCATCAGCGG TCATGGGACG GACAGCCGGC CAGCTATGGC CATCTTTGAG 960

CTGCTGGACT ATATTGTGAA CGAGCCGCCT CCCAAGCTGC CCAATGGTGT CTTCACCCAG 1020

GACTTCCAGG AGTTTGTAAA TAAATGCCTA ATAAAGAACC CAGCAGAGCG AGCAGATCTG 1080

AAGATGCTCA TGAGCCACAC GTTCATCAAG CGGTCCGAGG TGGAAGAGGT GGATTTTGCC 1140

GGCTGGTTGT GTAAAACGCT GCGGCTGAAT CAGCCCAGCA CGCCCACGCG CACCGCCGTG 1200

TGA 1203

Domain Profile

S: 2     ekleelGaGnggvVekvlhkksgkilavKtirleikkeeqkqilrelevlkkssnspyiv  61

         e+++elGaGnggvV+kv+h++sg i+a+K i+leik+++++qi+rel+vl+++ nspyiv

Q: 73    ERISELGAGNGGVVTKVQHRPSGLIMARKLIHLEIKPAIRNQIIRELQVLHEC-NSPYIV  131

         789**************************************************.******

S: 62    kfyGafvsegdvsicmelmdgtsldkvlkkvlekkkripekvlgkiavavvkaleylkek  121

         +fyGaf+s+g++sicme+mdg+sld+vlk+    +kripe++lgk+++av+++l+yl+ek

Q: 132   GFYGAFYSDGEISICMEHMDGGSLDQVLKE----AKRIPEEILGKVSIAVLRGLAYLREK  187

         *****************************9....79************************

S: 122   lkiiHRDvKPsNiLvnskGevKlCDFGvsgqlvdsiAksavGtksYmaPEriegkkkYsv  181

         ++i+HRDvKPsNiLvns+Ge+KlCDFGvsgql+ds+A+s+vGt+sYm+PEr++g ++Ysv

Q: 188   HQIMHRDVKPSNILVNSRGEIKLCDFGVSGQLIDSMANSFVGTRSYMSPERLQG-THYSV  246

         ******************************************************.*****

S: 182   ksdvWslGltlvelatgrypyeklke..................................  207

         +sd+Ws+Gl+lvel +gryp++++++

Q: 247   QSDIWSMGLSLVELSIGRYPIPPPDAkeleaifgrpmvdgiegephsisprprppgrpis  306

         ************************999*********************************

S: 208   ...........ilellkaivneeppklpeeefseefqefvskClkkdekeRpklkellkh  256

                    i+ell++ivne+ppklp+  f+++fqefv+kCl+k+++eR++lk+l++h

Q: 307   ghgtdsrpamaIFELLDYIVNEPPPKLPNGVFTQDFQEFVNKCLIKNPAERADLKMLMSH  366

         ************************************************************

S: 257   afi  259

+fi

Q: 367   TFI  369

*97

Domain Sequence
(FASTA)

ERISELGAGN GGVVTKVQHR PSGLIMARKL IHLEIKPAIR NQIIRELQVL HECNSPYIVG 60

FYGAFYSDGE ISICMEHMDG GSLDQVLKEA KRIPEEILGK VSIAVLRGLA YLREKHQIMH 120

RDVKPSNILV NSRGEIKLCD FGVSGQLIDS MANSFVGTRS YMSPERLQGT HYSVQSDIWS 180

MGLSLVELSI GRYPIPPPDA KELEAIFGRP MVDGIEGEPH SISPRPRPPG RPISGHGTDS 240

RPAMAIFELL DYIVNEPPPK LPNGVFTQDF QEFVNKCLIK NPAERADLKM LMSHTFI 297

Keyword

Acetylation; ATP-binding; Complete proteome; Kinase; Nucleotide-binding; Phosphoprotein; Reference proteome; Serine/threonine-protein kinase; Transferase; Tyrosine-protein kinase.

Sequence Source

Ensembl

Orthology

Ortholog group

Anolis carolinensis"; ?>Bos taurus"; ?>Callithrix jacchus"; ?>Cavia porcellus"; ?>Ciona intestinalis"; ?>Danio rerio"; ?>Dipodomys ordii"; ?>Drosophila melanogaster"; ?>Equus caballus"; ?>Felis catus"; ?>Gadus morhua"; ?>Gallus gallus"; ?>Gasterosteus aculeatus"; ?>Gorilla gorilla"; ?>Homo sapiens"; ?>Ictidomys tridecemlineatus"; ?>Loxodonta africana"; ?>Meleagris gallopavo"; ?>Monodelphis domestica"; ?>Mus musculus"; ?>Mustela putorius furo"; ?>Myotis lucifugus"; ?>Nomascus leucogenys"; ?>Oreochromis niloticus"; ?>Oryzias latipes"; ?>Otolemur garnettii"; ?>Pan troglodytes"; ?>Pelodiscus sinensis"; ?>Petromyzon marinus"; ?>Procavia capensis"; ?>Rattus norvegicus"; ?>Sarcophilus harrisii"; ?>Sus scrofa"; ?>Takifugu rubripes"; ?>Xiphophorus maculatus"; ?>Schizosaccharomyces pombe"; ?>

Gene Ontology

GO:0005778	; C:peroxisomal membrane
GO:0005524	; F:ATP binding
GO:0004674	; F:protein serine/threonine kinase activity
GO:0004713	; F:protein tyrosine kinase activity
GO:2000147	; P:positive regulation of cell motility

KEGG

cfa:611939

;

InterPros

IPR011009	; Kinase-like_dom.
IPR000719	; Prot_kinase_cat_dom.
IPR017441	; Protein_kinase_ATP_BS.
IPR002290	; Ser/Thr_dual-sp_kinase_dom.
IPR008271	; Ser/Thr_kinase_AS.

Pfam

PF00069

; Pkinase; 1.

SMARTs

SM00220

; S_TKc; 1.

Prosites

PS00107	; PROTEIN_KINASE_ATP; 1.
PS50011	; PROTEIN_KINASE_DOM; 1.
PS00108	; PROTEIN_KINASE_ST; 1.

Prints

Created Date

20-Feb-2013