EKS-DAR-00747

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EKS-DAR-00747

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
TK/Src	442.0	1.2E-132	268	516	249

Status

Reviewed

Ensembl Protein

ENSDARP00000093618

UniProt Accession

Q1JPZ3; Q6EWH0;

Protein Name

Proto-oncogene tyrosine-protein kinase Src

Protein Synonyms/Alias

Proto-oncogene c-Src; pp60c-src; p60-Src;

Gene Name

src

Gene Synonyms/Alias

src;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
ENSDARG00000008107	ENSDARP00000097596	ENSDART00000112198
ENSDARG00000008107	ENSDARP00000093618	ENSDART00000102843

Organism

Danio rerio

Functional Description

Non-receptor protein tyrosine kinase which is activatedfollowing engagement of many different classes of cellular receptors including immune response receptors, integrins and other adhesion receptors, receptor protein tyrosine kinases, G protein- coupled receptors as well as cytokine receptors. Participates in signaling pathways that control a diverse spectrum of biological activities including gene transcription, immune response, cell adhesion, cell cycle progression, apoptosis, migration, and transformation. Due to functional redundancy between members of the SRC kinase family, identification of the specific role of each src kinase is very difficult. Src appears to be one of the primary kinases activated following engagement of receptors and plays a role in the activation of other protein tyrosine kinase (PTK) families. Receptor clustering or dimerization leads to recruitment of src to the receptor complexes where it phosphorylates the tyrosine residues within the receptor cytoplasmic domains. Plays an important role in the regulation of cytoskeletal organization through phosphorylation of specific substrates involved in this process. When cells adhere via focal adhesions to the extra- cellular matrix, signals are transmitted by integrins into the cell and result in tyrosine phosphorylation of a number of focal adhesion proteins, including ptk2/fak1 and paxillin (pxn). Also active at the sites of cell-cell contact adherens junctions and at gap junctions. Implicated in the regulation of pre-mRNA- processing. Might be involved not only in mediating the transduction of mitogenic signals at the level of the plasma membrane but also in controlling progression through the cell cycle via interaction with regulatory proteins in the nucleus (By similarity).

Protein Length

534

Protein Sequence
(FASTA)

MGGVKSKPKE LGQRSRSLDD GTGGHHHHTP NPTSFTPNRS PPVEGSRRGT QPNIINAEQA 60

LFGGVNSTTN SITSPNRIGI LGGVTTFVAL YDYESRTASD LSFRKGERLQ IVNNTEGDWW 120

LARSLTTGES GYIPSNYVAP SDSIQAEEWY FGKITRRDSE RLLLNLENRR GTFLVRESET 180

TKGAYCLSVL DYDNVKGLNV KHYKIRKLDS GGFYITSRTQ FSTLQQLVNH YRQHADGLCH 240

SLTDVCPVLK PPTQGLARDA WEIPRDSLRL DVKLGQGCFG EVWMGTWNGT TRVAIKTLKP 300

GTMSPEAFLQ EAQVMKKLRH EKLVQLYAVV SEEPIYIVTE YMGQGSLLDF LKGDMGKMLR 360

LPQLVDMASQ IASGMAYVER MNYVHRDLRA ANILVGDNLV CKVADFGLAR LIEDNEYTAR 420

QGAKFPIKWT APEAALYGRF TIKSDVWSFG ILLTELTTKG RVPYPGMVNR EVLDQVERGY 480

RMPCPAECPD SLHELMLTCW RKEPEERPTF EYLQGFLEDY FTSTEPQYQP GENL 534

Nucleotide Sequence
(FASTA)

ATGGGTGGAG TCAAGAGTAA ACCCAAAGAG CTGGGCCAGC GCTCGCGGAG TCTGGACGAC 60

GGCACCGGAG GACACCACCA CCACACCCCC AACCCCACCT CCTTCACCCC TAACCGCAGT 120

CCACCGGTGG AGGGGTCCCG GCGCGGCACA CAGCCCAACA TCATAAACGC GGAGCAGGCG 180

CTCTTTGGGG GAGTGAACTC CACCACCAAC AGCATCACAT CTCCTAATCG AATCGGCATA 240

TTAGGCGGCG TGACCACATT TGTGGCTCTG TATGACTACG AGTCACGAAC CGCCTCCGAT 300

TTGTCATTCA GGAAAGGAGA ACGACTCCAG ATTGTCAATA ACACGGAGGG TGACTGGTGG 360

CTAGCGCGAT CACTGACGAC AGGAGAGAGC GGATACATTC CCAGCAATTA CGTGGCACCG 420

TCTGACTCCA TCCAGGCTGA AGAATGGTAC TTCGGAAAAA TCACACGCAG AGACTCTGAG 480

CGACTGCTCT TGAATCTGGA GAACAGACGA GGAACTTTCC TGGTCAGAGA GAGCGAGACC 540

ACCAAAGGTG CCTACTGTCT CTCCGTCCTG GATTACGACA ACGTGAAGGG CCTGAATGTA 600

AAACATTATA AGATCCGCAA ACTGGACAGC GGTGGATTTT ACATCACGTC CCGCACACAG 660

TTCAGCACAT TACAGCAGCT GGTCAACCAC TACCGCCAGC ACGCAGACGG CCTGTGCCAC 720

TCCCTGACTG ACGTGTGTCC AGTGCTGAAG CCTCCGACTC AGGGTCTGGC ACGTGATGCC 780

TGGGAGATTC CTCGAGACTC GCTGAGACTG GATGTCAAGC TGGGCCAGGG CTGCTTCGGG 840

GAGGTCTGGA TGGGCACCTG GAACGGGACG ACACGTGTGG CCATCAAGAC TCTGAAGCCT 900

GGCACCATGT CTCCCGAAGC GTTCCTGCAG GAGGCGCAAG TGATGAAGAA GCTCCGGCAT 960

GAGAAGCTGG TGCAGCTTTA CGCCGTCGTC TCAGAGGAGC CCATTTACAT AGTCACTGAG 1020

TACATGGGTC AAGGAAGCCT GCTGGATTTC CTGAAAGGTG ACATGGGGAA AATGCTGCGT 1080

TTGCCTCAGC TCGTGGACAT GGCCTCTCAG ATCGCCTCAG GAATGGCATA CGTGGAGAGA 1140

ATGAACTATG TGCACAGGGA CCTCAGGGCT GCCAACATCC TGGTGGGCGA TAACCTGGTG 1200

TGTAAAGTGG CTGATTTCGG CCTCGCTCGA CTCATTGAGG ACAACGAATA CACCGCGAGA 1260

CAAGGAGCAA AGTTTCCTAT TAAGTGGACT GCACCGGAGG CAGCTCTGTA CGGACGCTTC 1320

ACCATCAAAT CTGACGTGTG GTCGTTTGGG ATTTTACTGA CGGAGCTCAC CACCAAAGGC 1380

AGGGTGCCTT ATCCAGGTAT GGTGAACCGC GAGGTGCTTG ACCAGGTGGA GCGTGGCTAC 1440

AGGATGCCCT GCCCGGCCGA ATGCCCTGAC TCCCTGCACG AGCTCATGCT GACCTGCTGG 1500

CGCAAAGAGC CCGAAGAGCG GCCCACATTC GAGTACCTGC AGGGTTTCCT GGAGGACTAC 1560

TTCACCTCCA CCGAACCACA ATACCAACCC GGAGAAAACC TCTAG 1605

Domain Profile

S: 1     lklvkklGeGqfGevwlgkwkgsvkvavktlkegtlspeafleeaqilkklrheklvkly  60

         l+l  klG+G+fGevw+g+w+g+++va+ktlk+gt+speafl+eaq++kklrheklv+ly

Q: 268   LRLDVKLGQGCFGEVWMGTWNGTTRVAIKTLKPGTMSPEAFLQEAQVMKKLRHEKLVQLY  327

         57889*******************************************************

S: 61    avvseeePiyivtelmakGslldflkeeegkklklpklvdlaaqvaeGmayleeknlihr  120

         avvse ePiyivte+m +Gslldflk + gk+l+lp+lvd+a+q+a+Gmay+e++n++hr

Q: 328   AVVSE-EPIYIVTEYMGQGSLLDFLKGDMGKMLRLPQLVDMASQIASGMAYVERMNYVHR  386

         ****9.******************************************************

S: 121   dlaarnvlvgeslvvkvadfGlarlieddeytakegaklPikWtaPeaiklgkftiksdv  180

         dl+a+n+lvg++lv+kvadfGlarlied+eyta++gak+PikWtaPea+ +g+ftiksdv

Q: 387   DLRAANILVGDNLVCKVADFGLARLIEDNEYTARQGAKFPIKWTAPEAALYGRFTIKSDV  446

         ************************************************************

S: 181   WsfGillteivtkGkvPypGmtneevleqvergyrlprpeecpeelyelllecwkkdpee  240

         WsfGillte++tkG+vPypGm n+evl+qvergyr+p+p+ecp++l+el+l+cw+k+pee

Q: 447   WSFGILLTELTTKGRVPYPGMVNREVLDQVERGYRMPCPAECPDSLHELMLTCWRKEPEE  506

         ************************************************************

S: 241   rPtfetlqev  250

         rPtfe+lq +

Q: 507   RPTFEYLQGF  516

         *******976

Domain Sequence
(FASTA)

LRLDVKLGQG CFGEVWMGTW NGTTRVAIKT LKPGTMSPEA FLQEAQVMKK LRHEKLVQLY 60

AVVSEEPIYI VTEYMGQGSL LDFLKGDMGK MLRLPQLVDM ASQIASGMAY VERMNYVHRD 120

LRAANILVGD NLVCKVADFG LARLIEDNEY TARQGAKFPI KWTAPEAALY GRFTIKSDVW 180

SFGILLTELT TKGRVPYPGM VNREVLDQVE RGYRMPCPAE CPDSLHELML TCWRKEPEER 240

PTFEYLQGF 249

Keyword

ATP-binding; Cell adhesion; Cell cycle; Cell membrane; Complete proteome; Cytoplasm; Cytoskeleton; Kinase; Lipoprotein; Membrane; Mitochondrion; Mitochondrion inner membrane; Myristate; Nucleotide-binding; Nucleus; Phosphoprotein; Reference proteome; S-nitrosylation; SH2 domain; SH3 domain; Transferase; Tyrosine-protein kinase.

Sequence Source

Ensembl

Orthology

Ortholog group

Ailuropoda melanoleuca"; ?>Anolis carolinensis"; ?>Bos taurus"; ?>Callithrix jacchus"; ?>Canis familiaris"; ?>Cavia porcellus"; ?>Ciona intestinalis"; ?>Ciona savignyi"; ?>Dipodomys ordii"; ?>Equus caballus"; ?>Felis catus"; ?>Gadus morhua"; ?>Gallus gallus"; ?>Gasterosteus aculeatus"; ?>Homo sapiens"; ?>Ictidomys tridecemlineatus"; ?>Latimeria chalumnae"; ?>Loxodonta africana"; ?>Macaca mulatta"; ?>Meleagris gallopavo"; ?>Microcebus murinus"; ?>Monodelphis domestica"; ?>Mus musculus"; ?>Mustela putorius furo"; ?>Myotis lucifugus"; ?>Nomascus leucogenys"; ?>Oreochromis niloticus"; ?>Oryctolagus cuniculus"; ?>Oryzias latipes"; ?>Otolemur garnettii"; ?>Pan troglodytes"; ?>Pelodiscus sinensis"; ?>Pongo abelii"; ?>Rattus norvegicus"; ?>Sarcophilus harrisii"; ?>Sus scrofa"; ?>Takifugu rubripes"; ?>Tetraodon nigroviridis"; ?>Xenopus tropicalis"; ?>Xiphophorus maculatus"; ?>

Gene Ontology

GO:0005856	; C:cytoskeleton
GO:0005743	; C:mitochondrial inner membrane
GO:0005634	; C:nucleus
GO:0005886	; C:plasma membrane
GO:0005524	; F:ATP binding
GO:0004715	; F:non-membrane spanning protein tyrosine kinase activity
GO:0007155	; P:cell adhesion
GO:0007049	; P:cell cycle

KEGG

dre:325084

;

InterPros

IPR011009	; Kinase-like_dom.
IPR000719	; Prot_kinase_cat_dom.
IPR017441	; Protein_kinase_ATP_BS.
IPR001245	; Ser-Thr/Tyr_kinase_cat_dom.
IPR000980	; SH2.
IPR001452	; SH3_domain.
IPR008266	; Tyr_kinase_AS.
IPR020635	; Tyr_kinase_cat_dom.

Pfam

PF07714	; Pkinase_Tyr; 1.
PF00017	; SH2; 1.
PF00018	; SH3_1; 1.

SMARTs

SM00252	; SH2; 1.
SM00326	; SH3; 1.
SM00219	; TyrKc; 1.

Prosites

PS00107	; PROTEIN_KINASE_ATP; 1.
PS50011	; PROTEIN_KINASE_DOM; 1.
PS00109	; PROTEIN_KINASE_TYR; 1.
PS50001	; SH2; 1.
PS50002	; SH3; 1.

Prints

PR00401	; SH2DOMAIN.
PR00452	; SH3DOMAIN.
PR00109	; TYRKINASE.

Created Date

20-Feb-2013