EKS-DAR-00064

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EKS-DAR-00064

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
TKL/RAF	409.1	1.9E-122	468	724	257

Status

Unreviewed

Ensembl Protein

ENSDARP00000016253

UniProt Accession

Q1LYG2;

Protein Name

Protein Synonyms/Alias

V-raf murine sarcoma viral oncogene homolog B1;

Gene Name

braf

Gene Synonyms/Alias

braf;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
ENSDARG00000017661	ENSDARP00000106977	ENSDART00000131168
ENSDARG00000017661	ENSDARP00000016253	ENSDART00000023894
ENSDARG00000017661	ENSDARP00000048390	ENSDART00000048391

Organism

Danio rerio

Functional Description

Protein Length

777

Protein Sequence
(FASTA)

MAALSSAESP PPVFNGDTMN RDPERDPGLD ELGAGLEPAC PGEAAIPECQ QGIPEEIWNI 60

KQMIKLTQEH LEALLDKFGG EHNPPSIYLE AYEEYTSKLD ALQQREQQLL EAIGNGTEFC 120

SSPTPTLLDV KGQGIQSAPT APNTLAVLQT PTDATRGNPR SPQKPIVRVF LPNKQRTVVP 180

ARCGMTVRDS LKKALMMRGL IPECCAVYRV QDGEKKPIGW DTDISWLTGE ELHVEVLENV 240

PLTTHNFVRK TFFTLAFCDF CRKLLFQGFR CQTCGYKFHQ RCSTEVPLMC VNYDQLDLLL 300

ASKFLVHHPI TQEEVSSEGT TPISEMCPSL PPSESTGSLC HPTVSPSKSI PIPQSFRPGE 360

EDHRNQFGQR DRSSSAPNVH INTIEPVNID DLIRDQGLPR SDGGSTTGLS ATPPASLPGS 420

LPNVKVSKSP CQPRERKPSS SSEDRNKMKT LGRRDSSDDW EIPEGQITLG QRIGSGSFGT 480

VYKGKWHGDV AVKMLNVTAP TPQQLQAFKN EVGVLRKTRH VNILLFMGYT TKPQLAIVTQ 540

WCEGSSLYHH LHIIETKFEM IKLIDIARQT AQGMDYLHAK SIIHRDLKSN NIFLHEDLTV 600

KIGDFGLATV KSRWSGSHQF EQLSGSILWM APEVIRLQDK NPYSFQSDVY AFGIVLYELM 660

SGALPYSNIN NRDQIIFMVG RGYLSPDLSK VRSNCPKAMK RLMADCLKKK REERPLFPQI 720

LASIELLARS LPKIHRSASE PSLNRAGFQT EDFSLYTCAS PKTPIQAGGY GEFTAFK 777

Nucleotide Sequence
(FASTA)

ATGGCGGCGT TGAGCAGCGC CGAGTCCCCA CCGCCCGTCT TCAACGGAGA CACCATGAAC 60

CGCGATCCGG AGCGGGACCC GGGACTGGAC GAGCTGGGCG CGGGGCTCGA ACCCGCCTGT 120

CCCGGAGAAG CGGCGATCCC CGAATGTCAA CAGGGCATTC CCGAGGAGAT ATGGAATATT 180

AAACAGATGA TTAAGTTAAC TCAAGAGCAC CTAGAAGCCC TTTTAGATAA GTTTGGAGGA 240

GAACACAATC CACCGTCTAT ATATCTAGAG GCCTATGAGG AGTACACTAG TAAGCTGGAT 300

GCATTACAGC AGCGGGAGCA GCAGCTTTTG GAAGCTATTG GGAATGGGAC CGAGTTCTGC 360

TCCTCGCCGA CACCCACCCT GCTGGACGTG AAGGGACAGG GCATTCAGAG TGCCCCCACT 420

GCCCCAAACA CCCTAGCTGT CCTGCAGACG CCCACTGACG CAACACGAGG CAATCCCCGC 480

TCGCCCCAGA AACCCATCGT CAGGGTCTTC CTGCCCAACA AACAGAGGAC TGTGGTTCCA 540

GCTCGTTGTG GGATGACGGT CAGAGACTCC TTAAAGAAGG CTCTGATGAT GCGAGGCTTG 600

ATCCCAGAAT GCTGTGCAGT CTATAGAGTT CAGGATGGGG AGAAGAAGCC CATTGGCTGG 660

GACACAGACA TTTCCTGGTT GACGGGGGAG GAGCTTCACG TTGAAGTTTT AGAAAATGTG 720

CCACTGACGA CCCATAATTT TGTGCGAAAG ACCTTCTTCA CGCTGGCCTT CTGTGATTTT 780

TGCCGGAAGC TGTTGTTTCA AGGCTTCCGC TGTCAGACGT GTGGCTATAA GTTTCACCAG 840

CGCTGCAGCA CGGAGGTCCC ACTCATGTGT GTCAACTACG ATCAGCTGGA TTTATTGCTG 900

GCATCAAAAT TTCTGGTGCA CCACCCCATC ACCCAGGAGG AGGTGTCATC AGAGGGCACG 960

ACACCCATTT CAGAGATGTG CCCCTCGCTA CCACCATCCG AATCTACTGG CTCTCTGTGC 1020

CATCCTACTG TGTCCCCGTC CAAATCCATC CCTATCCCGC AATCTTTTAG ACCTGGAGAA 1080

GAGGACCACC GTAACCAGTT TGGTCAGAGG GATCGCTCCT CATCAGCCCC TAACGTCCAT 1140

ATCAACACCA TTGAGCCGGT CAACATTGAT GATTTGATTC GTGATCAAGG CTTACCGAGG 1200

TCAGATGGAG GCTCAACAAC AGGCCTGTCC GCCACTCCCC CTGCCTCCCT GCCAGGTTCC 1260

CTGCCCAATG TGAAGGTGTC CAAATCTCCG TGCCAACCGA GGGAGAGGAA GCCATCGTCT 1320

TCATCTGAGG ACCGCAATAA AATGAAAACT CTGGGTCGAC GGGACTCGAG CGATGATTGG 1380

GAGATTCCAG AAGGGCAGAT TACTCTGGGC CAGCGGATAG GATCTGGTTC TTTTGGGACA 1440

GTTTACAAGG GGAAATGGCA CGGTGACGTG GCGGTAAAGA TGTTGAATGT TACAGCTCCC 1500

ACTCCACAGC AGCTACAAGC CTTTAAGAAT GAAGTGGGTG TCCTCAGGAA AACCCGTCAT 1560

GTGAATATCC TGCTGTTCAT GGGCTACACC ACCAAACCCC AGCTGGCTAT TGTTACTCAG 1620

TGGTGCGAAG GCTCGAGTCT CTACCACCAC CTGCACATCA TCGAGACCAA GTTCGAGATG 1680

ATCAAGCTGA TCGACATTGC CCGACAGACA GCTCAGGGCA TGGACTATCT GCACGCAAAG 1740

TCCATCATCC ATAGAGATCT GAAGAGTAAC AACATCTTTT TGCACGAGGA TTTAACGGTA 1800

AAGATTGGAG ATTTCGGTCT GGCTACAGTG AAGTCCCGCT GGAGCGGATC ACACCAGTTT 1860

GAGCAACTTT CAGGCTCTAT CTTGTGGATG GCTCCAGAAG TGATCAGACT GCAGGATAAA 1920

AACCCATATA GTTTCCAGTC GGACGTCTAC GCATTCGGCA TAGTGCTTTA TGAGCTTATG 1980

TCAGGGGCTT TGCCTTATTC AAACATCAAC AACAGAGACC AGATTATCTT CATGGTTGGC 2040

CGGGGTTACT TGTCTCCTGA CTTGAGCAAG GTGCGCAGCA ATTGTCCTAA GGCCATGAAG 2100

AGACTTATGG CAGATTGTCT AAAGAAAAAG CGAGAAGAAA GACCTCTTTT CCCTCAGATC 2160

TTGGCCTCCA TCGAGTTATT GGCACGCTCT CTGCCCAAAA TCCACCGCAG CGCCTCTGAA 2220

CCGTCTCTGA ACCGCGCTGG CTTCCAGACG GAGGACTTCA GCCTATACAC CTGCGCTTCC 2280

CCCAAAACAC CCATTCAGGC CGGTGGCTAT GGTGAATTCA CAGCGTTTAA ATAG 2334

Domain Profile

S: 2     elaekiGsGrfGtvyrgkwhGdvavkllnveelseeqleaFkkevaaykktRhenivLFm  61

         +l+++iGsG+fGtvy+gkwhGdvavk+lnv++++++ql+aFk+ev +++ktRh+ni+LFm

Q: 468   TLGQRIGSGSFGTVYKGKWHGDVAVKMLNVTAPTPQQLQAFKNEVGVLRKTRHVNILLFM  527

         6899********************************************************

S: 62    GyvskpelaivtslckgssLykllheqetkldlaklidiarqiaqgmdYLhakkilhkDl  121

         Gy +kp+laivt++c+gssLy++lh  etk+++ klidiarq+aqgmdYLhak+i+h+Dl

Q: 528   GYTTKPQLAIVTQWCEGSSLYHHLHIIETKFEMIKLIDIARQTAQGMDYLHAKSIIHRDL  587

         ************************************************************

S: 122   ksknilleeglkvvitDFgllsvkrllsgrrekqleipkgwilylapeviralsidleqd  181

         ks+ni+l+e+l+v+i+DFgl++vk+++sg  ++q+e+ +g+il++apevir       qd

Q: 588   KSNNIFLHEDLTVKIGDFGLATVKSRWSG--SHQFEQLSGSILWMAPEVIRL------QD  639

         *****************************..*********************......**

S: 182   elpfskesDvyafGivlyellarelpyke.esadqilfavgrG.lkpdlskvksklpkel  239

         ++p+s++sDvyafGivlyel++++lpy++ +++dqi+f+vgrG l+pdlskv+s++pk++

Q: 640   KNPYSFQSDVYAFGIVLYELMSGALPYSNiNNRDQIIFMVGRGyLSPDLSKVRSNCPKAM  699

         ************************************************************

S: 240   kellleClkfekeeRPlftqilkkl  264

         k+l+++Clk+++eeRPlf+qil+++

Q: 700   KRLMADCLKKKREERPLFPQILASI  724

         *********************9875

Domain Sequence
(FASTA)

TLGQRIGSGS FGTVYKGKWH GDVAVKMLNV TAPTPQQLQA FKNEVGVLRK TRHVNILLFM 60

GYTTKPQLAI VTQWCEGSSL YHHLHIIETK FEMIKLIDIA RQTAQGMDYL HAKSIIHRDL 120

KSNNIFLHED LTVKIGDFGL ATVKSRWSGS HQFEQLSGSI LWMAPEVIRL QDKNPYSFQS 180

DVYAFGIVLY ELMSGALPYS NINNRDQIIF MVGRGYLSPD LSKVRSNCPK AMKRLMADCL 240

KKKREERPLF PQILASI 257

Keyword

ATP-binding; Complete proteome; Kinase; Metal-binding; Nucleotide-binding; Reference proteome; Serine/threonine-protein kinase; Transferase; Zinc.

Sequence Source

Ensembl

Orthology

Ortholog group

Ailuropoda melanoleuca"; ?>Anolis carolinensis"; ?>Bos taurus"; ?>Caenorhabditis elegans"; ?>Callithrix jacchus"; ?>Canis familiaris"; ?>Cavia porcellus"; ?>Ciona intestinalis"; ?>Drosophila melanogaster"; ?>Equus caballus"; ?>Felis catus"; ?>Gallus gallus"; ?>Gasterosteus aculeatus"; ?>Gorilla gorilla"; ?>Homo sapiens"; ?>Ictidomys tridecemlineatus"; ?>Latimeria chalumnae"; ?>Loxodonta africana"; ?>Meleagris gallopavo"; ?>Monodelphis domestica"; ?>Mus musculus"; ?>Mustela putorius furo"; ?>Myotis lucifugus"; ?>Nomascus leucogenys"; ?>Ornithorhynchus anatinus"; ?>Oryctolagus cuniculus"; ?>Oryzias latipes"; ?>Otolemur garnettii"; ?>Pan troglodytes"; ?>Pelodiscus sinensis"; ?>Petromyzon marinus"; ?>Pongo abelii"; ?>Rattus norvegicus"; ?>Sarcophilus harrisii"; ?>Sus scrofa"; ?>Taeniopygia guttata"; ?>Takifugu rubripes"; ?>Tetraodon nigroviridis"; ?>Vicugna pacos"; ?>Xenopus tropicalis"; ?>Xiphophorus maculatus"; ?>

Gene Ontology

GO:0005622	; C:intracellular
GO:0005524	; F:ATP binding
GO:0046872	; F:metal ion binding
GO:0004674	; F:protein serine/threonine kinase activity
GO:0005057	; F:receptor signaling protein activity
GO:0035556	; P:intracellular signal transduction

KEGG

InterPros

IPR020454	; DAG/PE-bd.
IPR011009	; Kinase-like_dom.
IPR002219	; Prot_Kinase_C-like_PE/DAG-bd.
IPR000719	; Prot_kinase_cat_dom.
IPR017441	; Protein_kinase_ATP_BS.
IPR003116	; Raf-like_ras-bd.
IPR001245	; Ser-Thr/Tyr_kinase_cat_dom.
IPR008271	; Ser/Thr_kinase_AS.

Pfam

PF00130	; C1_1; 1.
PF07714	; Pkinase_Tyr; 1.
PF02196	; RBD; 1.

SMARTs

SM00109	; C1; 1.
SM00455	; RBD; 1.

Prosites

PS00107	; PROTEIN_KINASE_ATP; 1.
PS50011	; PROTEIN_KINASE_DOM; 1.
PS00108	; PROTEIN_KINASE_ST; 1.
PS50898	; RBD; 1.
PS00479	; ZF_DAG_PE_1; 1.
PS50081	; ZF_DAG_PE_2; 1.

Prints

PR00008

; DAGPEDOMAIN.

Created Date

20-Feb-2013