EKS-DAR-00459

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EKS-DAR-00459

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
AGC/PKC	478.4	1.3E-143	339	597	259

Status

Reviewed

Ensembl Protein

ENSDARP00000038918

UniProt Accession

Q7SY24;

Protein Name

Protein kinase C beta type

Protein Synonyms/Alias

PKC-B; PKC-beta;

Gene Name

prkcbb

Gene Synonyms/Alias

prkcbb; prkcb1;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
ENSDARG00000022254	ENSDARP00000038918	ENSDART00000029451

Organism

Danio rerio

Functional Description

Calcium-activated and phospholipid-dependentserine/threonine-protein kinase involved in various processes such as regulation of the B-cell receptor (BCR) signalosome, apoptosis and transcription regulation. Plays a key role in B-cell activation and function by regulating BCR-induced NF-kappa-B activation and B-cell suvival. Required for recruitment and activation of the IKK kinase to lipid rafts and mediates phosphorylation of card11/carma1, leading to activate the NF- kappa-B signaling. Involved in apoptosis following oxidative damage: in case of oxidative conditions, specifically phosphorylates isoform p66Shc of shc1, leading to mitochondrial accumulation of p66Shc, where p66Shc acts as a reactive oxygen species producer. Acts as a coactivator of androgen receptor (andr)-dependent transcription, by being recruited to ANDR target genes and specifically mediating phosphorylation of 'Thr-6' of histone H3 (H3T6ph), a specific tag for epigenetic transcriptional activation (By similarity).

Protein Length

670

Protein Sequence
(FASTA)

MAEPANSDGE ERSAPMRGFA RQGALRQKNV HEVKNHKFIA RFFKQPTFCS HCTDFIWGFG 60

KQGFQCQVCC FVVHKRCHEF VTFSCPGADK GPASDDPRSK HKFKVHTYSS PTFCDHCGSL 120

LYGLIHQGMR CDHCMMNIHK RCVANVPSLC GTDHTERRGR IQITAEIKNN VLTVSIKEAK 180

NLVPMDPNGL SDPYVKLKLI PDPKSESKQK TKTIKCCLNP TWNETFTFNL KESDKDRRLS 240

VEIWDWDLTS RNDFMGSLSF GISELQKQGV DGWFKLLSQE EGEYFNVPVP PEGEEGNEEL 300

RQKFERAKIG PSKTDGSSSN AISKFDSNGN RDRMKLSDFN FLMVLGKGSF GKVMLAERKG 360

ADELFAIKIL KKDVVIQDDD VECTMVEKRV LALSGKPPFL TQLHSCFQTM DRLYFVMEYI 420

NGGDLMYHIQ QVGKFKEPHA VFYAAEIAIG LFFLHSKGVI YRDLKLDNVM LDAEGHIKIA 480

DFGMCKENML DGVTTKTFCG TPDYIAPEII AYQPYGKSVD WWAFGVLLYE MLAGQPPFDG 540

EDEDELFQSI MEHHVSYPKS MSKEAVAICK GLMTKHPGKR LGCGPEGERD IREHGFFRYM 600

DWEKLEHREV QPPFKPKACG RDAENFDRFF TRHPPVLTPP DQEVIMNLDQ DEFEGFSFIN 660

PEFPAMEAQS 670

Nucleotide Sequence
(FASTA)

ATGGCAGAGC CGGCGAACAG CGATGGAGAG GAGAGGAGCG CACCCATGAG GGGTTTTGCC 60

CGGCAAGGCG CCCTGAGACA GAAGAACGTT CACGAAGTCA AGAACCACAA GTTTATCGCT 120

CGCTTCTTCA AGCAGCCTAC TTTCTGCAGC CACTGCACGG ACTTTATCTG GGGTTTTGGG 180

AAACAAGGAT TTCAATGTCA AGTCTGCTGC TTTGTGGTGC ACAAGCGTTG CCATGAATTC 240

GTCACCTTCT CCTGCCCGGG AGCAGATAAG GGTCCCGCAT CAGATGACCC GCGCAGTAAG 300

CACAAGTTCA AGGTCCACAC ATACTCCAGT CCGACCTTCT GTGACCACTG CGGCTCTCTG 360

CTGTACGGCC TCATCCACCA GGGCATGAGA TGTGACCACT GCATGATGAA CATCCACAAG 420

CGCTGTGTGG CCAACGTGCC GAGTCTGTGT GGGACTGACC ACACCGAGAG GAGGGGCCGC 480

ATCCAAATCA CTGCAGAGAT CAAGAACAAT GTGCTCACCG TCAGCATTAA AGAGGCAAAG 540

AACCTGGTAC CCATGGACCC CAATGGCCTC TCTGACCCCT ACGTCAAACT AAAACTCATC 600

CCTGACCCCA AGAGCGAGAG CAAACAGAAG ACCAAGACCA TCAAGTGCTG CCTGAATCCC 660

ACATGGAACG AGACCTTCAC ATTTAATCTG AAGGAGTCTG ACAAGGACCG CAGGCTGTCT 720

GTGGAGATCT GGGACTGGGA CTTGACCAGC CGGAACGACT TTATGGGCTC TCTTTCTTTT 780

GGCATTTCAG AGCTTCAGAA GCAAGGAGTG GACGGATGGT TTAAGCTGCT CAGTCAGGAA 840

GAGGGCGAGT ATTTCAATGT ACCTGTCCCA CCTGAGGGAG AAGAGGGCAA CGAGGAACTC 900

CGACAGAAGT TTGAGAGAGC TAAAATCGGT CCCAGCAAAA CAGATGGCTC CTCCTCTAAC 960

GCCATCTCCA AATTCGACAG TAATGGCAAC CGGGACCGCA TGAAACTGTC TGACTTCAAT 1020

TTCCTCATGG TTCTAGGAAA GGGTAGCTTT GGAAAGGTAA TGTTGGCTGA GAGGAAGGGA 1080

GCGGATGAGC TGTTTGCCAT AAAGATCCTG AAGAAGGACG TGGTGATCCA GGACGATGAT 1140

GTCGAATGCA CTATGGTGGA GAAGAGAGTG CTGGCTTTGT CAGGAAAACC CCCGTTCCTA 1200

ACCCAGCTGC ACTCCTGCTT TCAGACAATG GACCGCCTGT ACTTTGTCAT GGAATACATC 1260

AACGGTGGAG ATCTCATGTA CCATATCCAG CAGGTCGGCA AGTTTAAGGA GCCCCATGCT 1320

GTGTTTTATG CTGCAGAAAT TGCCATAGGC CTGTTCTTCC TGCATTCAAA GGGTGTTATT 1380

TACAGAGATC TGAAGTTGGA TAATGTCATG CTGGATGCAG AGGGTCATAT CAAAATCGCT 1440

GACTTTGGCA TGTGCAAAGA GAACATGCTT GATGGAGTCA CCACCAAGAC CTTCTGTGGA 1500

ACCCCTGACT ACATCGCCCC TGAGATCATC GCCTACCAGC CGTATGGAAA GTCAGTGGAC 1560

TGGTGGGCTT TTGGAGTTTT GCTATATGAG ATGCTGGCTG GGCAGCCTCC GTTTGATGGC 1620

GAGGATGAGG ATGAGCTATT TCAGTCCATC ATGGAGCACC ATGTTTCCTA TCCCAAATCC 1680

ATGTCCAAGG AGGCTGTGGC CATCTGTAAA GGGCTGATGA CCAAACACCC AGGCAAGCGT 1740

CTGGGCTGCG GTCCAGAAGG TGAGCGAGAC ATCAGGGAAC ATGGCTTCTT CCGTTACATG 1800

GACTGGGAGA AGCTGGAGCA CAGGGAAGTT CAGCCACCAT TTAAACCAAA AGCCTGTGGC 1860

CGGGATGCAG AGAACTTTGA CCGCTTTTTC ACCCGCCATC CACCGGTTCT CACCCCTCCA 1920

GACCAGGAGG TCATCATGAA CCTCGACCAA GACGAATTCG AGGGCTTTTC CTTCATAAAC 1980

CCTGAATTCC CTGCCATGGA AGCCCAGAGT TAA 2013

Domain Profile

S: 1     fellkvlGkGsfgkvllaelkktdelyaikvlkkdvvlqdddveltlvekrvlalaskkp  60

         f++l+vlGkGsfgkv+lae+k+ del+aik+lkkdvv+qdddve+t+vekrvlal+ k+p

Q: 339   FNFLMVLGKGSFGKVMLAERKGADELFAIKILKKDVVIQDDDVECTMVEKRVLALSGKPP  398

         99**********************************************************

S: 61    flvqlfscfqtedrlffvleyvnGGdlmfhiqkarklkeerarfyaaeiilalkflhekg  120

         fl+ql+scfqt+drl+fv+ey+nGGdlm+hiq+++k+ke++a+fyaaei+++l+flh+kg

Q: 399   FLTQLHSCFQTMDRLYFVMEYINGGDLMYHIQQVGKFKEPHAVFYAAEIAIGLFFLHSKG  458

         ************************************************************

S: 121   iiyrdlkldnvlldaeGhikladfGlckeeilegkttstfcGtPdyiaPeilkeeeygks  180

         +iyrdlkldnv+ldaeGhik+adfG+cke++l+g tt+tfcGtPdyiaPei+++++ygks

Q: 459   VIYRDLKLDNVMLDAEGHIKIADFGMCKENMLDGVTTKTFCGTPDYIAPEIIAYQPYGKS  518

         ************************************************************

S: 181   vdwwalGvllyemlagqsPfegededelfesilekevlyPkslskeaveilkglltkdpe  240

         vdwwa+Gvllyemlagq+Pf+gededelf+si+e++v+yPks+skeav+i+kgl+tk+p

Q: 519   VDWWAFGVLLYEMLAGQPPFDGEDEDELFQSIMEHHVSYPKSMSKEAVAICKGLMTKHPG  578

         ************************************************************

S: 241   krlGvkeegeedikehaff  259

         krlG+ +ege+di+eh ff

Q: 579   KRLGCGPEGERDIREHGFF  597

         ******************9

Domain Sequence
(FASTA)

FNFLMVLGKG SFGKVMLAER KGADELFAIK ILKKDVVIQD DDVECTMVEK RVLALSGKPP 60

FLTQLHSCFQ TMDRLYFVME YINGGDLMYH IQQVGKFKEP HAVFYAAEIA IGLFFLHSKG 120

VIYRDLKLDN VMLDAEGHIK IADFGMCKEN MLDGVTTKTF CGTPDYIAPE IIAYQPYGKS 180

VDWWAFGVLL YEMLAGQPPF DGEDEDELFQ SIMEHHVSYP KSMSKEAVAI CKGLMTKHPG 240

KRLGCGPEGE RDIREHGFF 259

Keyword

Adaptive immunity; Apoptosis; ATP-binding; Calcium; Chromatin regulator; Complete proteome; Cytoplasm; Immunity; Kinase; Membrane; Metal-binding; Nucleotide-binding; Nucleus; Phosphoprotein; Reference proteome; Repeat; Serine/threonine-protein kinase; Transcription; Transcription regulation; Transferase; Zinc; Zinc-finger.

Sequence Source

Ensembl

Orthology

Ortholog group

Bos taurus"; ?>Caenorhabditis elegans"; ?>Canis familiaris"; ?>Cavia porcellus"; ?>Drosophila melanogaster"; ?>Felis catus"; ?>Gadus morhua"; ?>Gasterosteus aculeatus"; ?>Homo sapiens"; ?>Ictidomys tridecemlineatus"; ?>Latimeria chalumnae"; ?>Macaca mulatta"; ?>Mus musculus"; ?>Myotis lucifugus"; ?>Nomascus leucogenys"; ?>Ochotona princeps"; ?>Oreochromis niloticus"; ?>Oryctolagus cuniculus"; ?>Otolemur garnettii"; ?>Pan troglodytes"; ?>Pongo abelii"; ?>Rattus norvegicus"; ?>Sarcophilus harrisii"; ?>Tetraodon nigroviridis"; ?>Xenopus tropicalis"; ?>Xiphophorus maculatus"; ?>

Gene Ontology

GO:0005737	; C:cytoplasm
GO:0016020	; C:membrane
GO:0005634	; C:nucleus
GO:0050681	; F:androgen receptor binding
GO:0005524	; F:ATP binding
GO:0003682	; F:chromatin binding
GO:0042393	; F:histone binding
GO:0035403	; F:histone kinase activity (H3-T6 specific)
GO:0030374	; F:ligand-dependent nuclear receptor transcription coactivator activity
GO:0004697	; F:protein kinase C activity
GO:0008270	; F:zinc ion binding
GO:0006915	; P:apoptotic process
GO:0042113	; P:B cell activation
GO:0050853	; P:B cell receptor signaling pathway
GO:0035162	; P:embryonic hemopoiesis
GO:0035556	; P:intracellular signal transduction
GO:0043123	; P:positive regulation of I-kappaB kinase/NF-kappaB cascade
GO:0006357	; P:regulation of transcription from RNA polymerase II promoter
GO:0006351	; P:transcription, DNA-dependent

KEGG

dre:393953

;

InterPros

IPR000961	; AGC-kinase_C.
IPR000008	; C2_Ca-dep.
IPR008973	; C2_Ca/lipid-bd_dom_CaLB.
IPR020477	; C2_dom.
IPR018029	; C2_membr_targeting.
IPR020454	; DAG/PE-bd.
IPR011009	; Kinase-like_dom.
IPR017892	; Pkinase_C.
IPR002219	; Prot_Kinase_C-like_PE/DAG-bd.
IPR000719	; Prot_kinase_cat_dom.
IPR017441	; Protein_kinase_ATP_BS.
IPR014375	; Protein_kinase_C_a/b/g.
IPR002290	; Ser/Thr_dual-sp_kinase_dom.
IPR008271	; Ser/Thr_kinase_AS.

Pfam

PF00130	; C1_1; 2.
PF00168	; C2; 1.
PF00069	; Pkinase; 1.
PF00433	; Pkinase_C; 1.

SMARTs

SM00109	; C1; 2.
SM00239	; C2; 1.
SM00133	; S_TK_X; 1.
SM00220	; S_TKc; 1.

Prosites

PS51285	; AGC_KINASE_CTER; 1.
PS50004	; C2; 1.
PS00107	; PROTEIN_KINASE_ATP; 1.
PS50011	; PROTEIN_KINASE_DOM; 1.
PS00108	; PROTEIN_KINASE_ST; 1.
PS00479	; ZF_DAG_PE_1; 2.
PS50081	; ZF_DAG_PE_2; 2.

Prints

PR00360	; C2DOMAIN.
PR00008	; DAGPEDOMAIN.

Created Date

20-Feb-2013