EKS-EQC-00119

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EKS-EQC-00119

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
TK/Met	479.5	2.7E-144	1078	1338	261

Status

Reviewed

Ensembl Protein

ENSECAP00000008993

UniProt Accession

Q2QLA9;

Protein Name

Hepatocyte growth factor receptor

Protein Synonyms/Alias

HGF receptor; HGF/SF receptor; Proto-oncogene c-Met; Scatter factor receptor; SF receptor; Tyrosine-protein kinase Met;

Gene Name

MET

Gene Synonyms/Alias

MET;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
ENSECAG00000010385	ENSECAP00000008993	ENSECAT00000011493

Organism

Equus caballus

Functional Description

Receptor tyrosine kinase that transduces signals fromthe extracellular matrix into the cytoplasm by binding to hepatocyte growth factor/HGF ligand. Regulates many physiological processes including proliferation, scattering, morphogenesis and survival. Ligand binding at the cell surface induces autophosphorylation of MET on its intracellular domain that provides docking sites for downstream signaling molecules. Following activation by ligand, interacts with the PI3-kinase subunit PIK3R1, PLCG1, SRC, GRB2, STAT3 or the adapter GAB1. Recruitment of these downstream effectors by MET leads to the activation of several signaling cascades including the RAS-ERK, PI3 kinase-AKT, or PLCgamma-PKC. The RAS-ERK activation is associated with the morphogenetic effects while PI3K/AKT coordinates prosurvival effects. During embryonic development, MET signaling plays a role in gastrulation, development and migration of muscles and neuronal precursors, angiogenesis and kidney formation. In adults, participates in wound healing as well as organ regeneration and tissue remodeling. Promotes also differentiation and proliferation of hematopoietic cells (By similarity).

Protein Length

1381

Protein Sequence
(FASTA)

MKAPAVLAPG ILVLLFTLVQ KSDGECKEAL VKSEMNVNMK YQLPNFTAET PIQNVVLHKH 60

HIYLGATNYI YVLNDKDLQK VAEYKTGPVL EHPDCFPCQD CSRKANLSGG AWKDNINMAL 120

LVDTYYDDQL ISCGSVHRGT CQRHVLPLNN VADIQSEVYC MYSPQAEEPH QCPDCVVSAL 180

GTKVLLSEKD RFVTFFVGNT INSSYLPDHS LHSISVRRLK ETQDGFKFLT DQSYIDVLPE 240

FRDSYPIKYI HAFESNHFIY FLTVQRETLD AQTFHTRIIR FCSVDSGLHS YMEMPLECIL 300

TEKRRKRSTS EEVFNILQAA YVSKPGAHLA KQIGANLNDD ILYGVFAQSK PDSAEPMNRS 360

AVCAFPVKYV NEFFNKIVNK NNVRCLQHFY GPHHEHCFNR TLLRNSSGCE VRNDEYRTEF 420

TTALQRVDLF MGQFNQVLLT SISTFIKGNL TIANLGTSEG RFMQVVVSRS GSSTPHVNFH 480

LDSHPVSPEV IVEHPLNQNG YTLVVTGKKI TKIPLNGLGC EHFQSCSQCL SAPPFVQCGW 540

CHDKCVRLEE CHNGTWTQEI CLPTIYKVFP TSAPLEGGTT LTVCGWDFGF RKNNKLDSKK 600

TKVLLGNESC TLTLSESTSN TLKCTVGPAM NERFNISITV SNSRGTARYS TFSYVDPIIT 660

SISPSYGPKT GGTLLTLTGK YLNSGNSRHI SIGGKTCTLK SVSDSILECY TPAQTTPTEF 720

PVKLKIDLAN REMNSFSYRE DPIVYEIHPT KSFISGGSTI TGVGKNLNSV SVLRMVINVR 780

EAGRNFTVAC QHRSNSEIIC CTTPSLQQLN LQLPLKTKAF FMLDGIHSKY FDLIYVHNPV 840

FKPFEKPVMI SIGNENVLEI KGNDIDPEAV KGEVLKVGNK SCENIHSHSE AVLCTVPSDL 900

LKLNSELNIE WKQAVSSTIL GKVIVQPDQN FTGLIVGVVS ISIILLLLLG LFLWLKRRKQ 960

IKDLGSELVR YDARVHTPHL DRLVSARSVS PTTEMVSNES VDYRATFPED QFPNSSQNGS 1020

CRQVQYPLTD LSPILTSGDS DISSPLLQNT VHIDLSALNP ELVQAVQHVV IGPSSLIVHF 1080

NEVIGRGHFG CVYHGTLLDN DDKKIHCAVK SLNRITDIGE VSQFLTEGII MKDFSHPNVL 1140

SLLGICLRSE GSPLVVLPYM KHGDLRNFIR NETHNPTVKD LIGFGLQVAK GMKYLASKKF 1200

VHRDLAARNC MLDEKFTVKV ADFGLARDMY DKEYYSVHNK TGAKLPVKWM ALESLQTQKF 1260

TTKSDVWSFG VLLWELMTRG APPYPDVNTF DITVYLLQGR RLLQPEYCPD PLYEVMLKCW 1320

HPKAELRPSF SELVSRISAI FSTFIGEHYV HVNATYVNVK CVAPYPSLLS SQDNVDGEVD 1380

T 1381

Nucleotide Sequence
(FASTA)

ATGAAGGCAC CTGCTGTGCT TGCACCTGGC ATCCTTGTGC TTCTGTTTAC CTTGGTGCAG 60

AAGAGCGATG GGGAGTGTAA AGAGGCGCTA GTAAAGTCCG AGATGAATGT GAACATGAAG 120

TATCAGCTTC CCAACTTCAC TGCAGAAACA CCCATCCAGA ACGTCGTTTT ACACAAGCAT 180

CACATTTACC TCGGGGCCAC TAACTACATT TATGTTTTAA ATGACAAAGA TCTTCAGAAA 240

GTTGCTGAGT ACAAGACTGG GCCCGTGCTG GAACACCCAG ATTGTTTCCC ATGTCAGGAC 300

TGCAGCCGCA AAGCCAATTT ATCAGGTGGC GCTTGGAAAG ATAACATCAA CATGGCTCTG 360

CTTGTTGACA CATACTATGA CGATCAACTC ATTAGTTGCG GCAGCGTCCA CAGAGGGACC 420

TGCCAGCGGC ACGTCCTTCC ACTCAACAAT GTTGCTGACA TACAGTCAGA AGTTTATTGC 480

ATGTACTCTC CACAAGCAGA AGAGCCCCAC CAGTGTCCTG ACTGTGTGGT GAGTGCCCTG 540

GGAACCAAAG TCCTGCTGTC TGAAAAGGAC CGATTTGTCA CCTTCTTCGT GGGCAATACC 600

ATAAATTCTT CTTACCTTCC AGATCATTCA TTGCATTCGA TATCGGTGAG AAGGCTAAAG 660

GAAACGCAAG ATGGTTTTAA GTTTTTGACA GATCAGTCCT ATATTGATGT TCTACCTGAG 720

TTCCGAGATT CTTACCCCAT TAAGTATATC CACGCCTTTG AAAGCAACCA TTTTATTTAC 780

TTTTTAACGG TCCAACGGGA AACTCTAGAT GCTCAGACTT TTCACACAAG AATAATCAGG 840

TTCTGTTCCG TGGACTCTGG ATTGCATTCC TACATGGAAA TGCCTCTGGA GTGTATTCTC 900

ACAGAAAAGA GAAGAAAGAG ATCCACAAGT GAGGAAGTGT TTAATATCCT CCAAGCTGCG 960

TATGTCAGTA AGCCTGGGGC TCATCTTGCT AAGCAAATAG GTGCCAACCT GAATGATGAC 1020

ATTCTCTATG GGGTGTTTGC ACAGAGCAAG CCAGATTCTG CTGAACCAAT GAATCGCTCT 1080

GCCGTCTGTG CATTCCCTGT CAAATATGTC AATGAATTCT TCAACAAGAT TGTCAACAAA 1140

AACAACGTGA GATGTCTCCA GCATTTTTAT GGACCCCACC ATGAACACTG CTTCAATAGG 1200

ACACTTTTGA GAAATTCATC AGGCTGTGAA GTGCGCAATG ATGAATATCG AACGGAGTTT 1260

ACCACAGCTT TGCAGCGCGT TGACTTGTTC ATGGGCCAAT TCAACCAAGT CCTCTTGACA 1320

TCTATATCCA CCTTCATTAA AGGAAACCTC ACTATAGCTA ATCTTGGGAC ATCAGAAGGT 1380

CGCTTCATGC AGGTTGTGGT TTCTCGATCA GGATCGTCAA CCCCTCATGT GAATTTTCAC 1440

CTGGATTCCC ACCCTGTGTC TCCAGAAGTG ATTGTGGAAC ACCCATTAAA CCAAAATGGT 1500

TACACACTGG TTGTCACCGG GAAAAAGATC ACCAAGATCC CACTGAATGG CTTGGGCTGT 1560

GAACATTTCC AGTCCTGCAG TCAGTGTCTC TCCGCCCCTC CCTTTGTGCA GTGTGGCTGG 1620

TGCCACGACA AATGTGTGCG ATTGGAGGAA TGCCACAACG GAACATGGAC TCAAGAGATC 1680

TGTCTGCCTA CAATCTACAA GGTTTTCCCA ACCAGTGCCC CCCTCGAAGG AGGGACAACG 1740

CTGACAGTAT GTGGCTGGGA CTTTGGATTC AGGAAGAATA ATAAACTTGA TTCAAAGAAA 1800

ACCAAAGTTC TCCTTGGAAA TGAGAGCTGC ACCTTGACTT TAAGTGAGAG CACGTCAAAT 1860

ACGTTGAAAT GCACAGTTGG TCCTGCAATG AATGAGCGTT TCAATATATC CATAACTGTT 1920

TCCAACAGTC GAGGGACAGC ACGATATAGT ACATTTTCCT ATGTGGATCC TATAATAACA 1980

AGTATTTCTC CAAGTTATGG TCCTAAGACT GGTGGCACTT TACTTACTTT AACTGGAAAG 2040

TACTTAAACA GTGGGAATTC TAGACACATT TCAATTGGTG GAAAAACATG TACCTTAAAA 2100

AGTGTGTCAG ATAGTATTCT TGAATGTTAT ACTCCAGCCC AAACCACTCC AACTGAGTTT 2160

CCCGTTAAAC TGAAAATTGA CTTAGCCAAC CGAGAGATGA ACAGCTTCAG TTACCGGGAA 2220

GACCCCATTG TCTATGAAAT TCATCCAACC AAATCTTTTA TTAGTGGTGG GAGCACAATT 2280

ACAGGTGTTG GAAAAAATCT GAATTCGGTT AGTGTCCTGA GGATGGTGAT AAATGTGCGT 2340

GAAGCAGGAA GGAACTTTAC AGTGGCATGT CAGCATCGCT CTAATTCAGA GATAATCTGC 2400

TGTACAACTC CTTCACTACA ACAGCTGAAT TTGCAACTCC CTCTGAAAAC CAAAGCCTTC 2460

TTCATGTTGG ACGGGATCCA TTCCAAATAC TTTGATCTCA TTTATGTACA TAATCCTGTG 2520

TTTAAGCCTT TTGAAAAGCC AGTGATGATC TCAATAGGCA ATGAAAATGT ACTGGAAATT 2580

AAGGGAAATG ATATTGACCC TGAAGCCGTT AAAGGTGAAG TGTTAAAAGT TGGAAATAAG 2640

AGCTGTGAGA ATATACACTC ACATTCTGAA GCCGTTTTAT GTACAGTCCC CAGTGACCTG 2700

CTGAAATTGA ACAGCGAGCT AAATATAGAG TGGAAGCAAG CAGTTTCTTC AACCATCCTT 2760

GGAAAAGTAA TAGTTCAACC AGATCAGAAT TTCACAGGAT TGATTGTTGG TGTTGTCTCA 2820

ATATCAATAA TACTCTTATT ATTACTCGGG CTTTTCCTGT GGCTGAAAAG GAGAAAGCAA 2880

ATTAAAGATC TGGGCAGTGA ATTAGTTCGC TATGATGCAA GAGTACACAC TCCTCATTTG 2940

GATAGGCTTG TAAGTGCCCG AAGTGTAAGC CCAACTACAG AAATGGTTTC AAATGAATCT 3000

GTAGACTACC GAGCTACTTT TCCAGAAGAC CAGTTTCCTA ACTCATCTCA GAATGGATCA 3060

TGCAGACAAG TGCAGTATCC TCTGACGGAC CTATCCCCCA TCCTAACTAG TGGGGACTCT 3120

GATATATCCA GTCCATTACT GCAAAATACC GTCCACATTG ACCTCAGTGC TCTAAATCCA 3180

GAGCTGGTGC AGGCAGTCCA GCACGTAGTG ATTGGGCCTA GTAGTCTGAT TGTGCATTTC 3240

AATGAAGTCA TAGGAAGAGG ACATTTTGGG TGTGTATATC ATGGGACTTT GTTGGACAAT 3300

GATGACAAGA AAATTCACTG TGCTGTGAAA TCCTTGAATA GAATCACTGA CATAGGAGAA 3360

GTTTCCCAGT TTCTCACCGA GGGAATTATC ATGAAAGATT TTAGTCATCC CAACGTTCTC 3420

TCGCTCTTGG GAATCTGCCT TCGAAGTGAG GGATCTCCAC TGGTGGTTCT ACCGTACATG 3480

AAACATGGAG ACCTTCGAAA TTTCATTAGA AATGAGACCC ACAACCCAAC TGTAAAAGAT 3540

CTTATTGGCT TTGGTCTTCA AGTAGCCAAA GGCATGAAAT ATCTTGCAAG CAAAAAGTTT 3600

GTCCACAGAG ACTTGGCTGC AAGAAACTGT ATGCTGGATG AAAAATTCAC TGTCAAGGTT 3660

GCTGATTTTG GGCTTGCCAG AGACATGTAT GATAAAGAAT ACTATAGTGT GCACAACAAA 3720

ACAGGTGCAA AACTGCCTGT GAAGTGGATG GCTTTAGAGA GTCTGCAAAC TCAGAAGTTT 3780

ACCACCAAGT CAGATGTGTG GTCCTTTGGC GTGCTCCTCT GGGAGCTGAT GACAAGAGGA 3840

GCACCACCTT ATCCCGATGT CAACACCTTT GATATAACAG TTTACTTGTT GCAAGGCAGA 3900

AGGCTCTTAC AACCAGAGTA CTGCCCAGAT CCCTTGTATG AAGTGATGCT AAAATGCTGG 3960

CACCCTAAAG CTGAACTGCG CCCATCCTTT TCTGAACTGG TCTCCAGGAT ATCAGCGATA 4020

TTCTCTACTT TCATCGGGGA GCACTACGTC CATGTGAACG CTACTTATGT GAATGTGAAA 4080

TGTGTTGCTC CATATCCTTC TCTGTTGTCA TCACAAGATA ACGTTGATGG CGAGGTAGAT 4140

ACATGA 4146

Domain Profile

S: 1     vdkeeviGkGhfGvvykgelsdsaskkikvavksleritdiekveeflreglvmkeldhp  60

         v+++eviG+GhfG+vy+g+l+d+++kki++avksl+ritdi++v++fl+eg++mk+++hp

Q: 1078  VHFNEVIGRGHFGCVYHGTLLDNDDKKIHCAVKSLNRITDIGEVSQFLTEGIIMKDFSHP  1137

         799*********************************************************

S: 61    nvlsllGialddeglplvvlpymkkGdlksfirneernltvkdllefalqvakgmeylas  120

         nvlsllGi+l++eg+plvvlpymk+Gdl++firne++n+tvkdl++f+lqvakgm+ylas

Q: 1138  NVLSLLGICLRSEGSPLVVLPYMKHGDLRNFIRNETHNPTVKDLIGFGLQVAKGMKYLAS  1197

         ************************************************************

S: 121   kkfvhrdlaarnclldekltvkvadfGlardvldkelyvveeerdarlpvkwlaleslqt  180

         kkfvhrdlaarnc+ldek+tvkvadfGlard++dke+y+v+++++a+lpvkw+aleslqt

Q: 1198  KKFVHRDLAARNCMLDEKFTVKVADFGLARDMYDKEYYSVHNKTGAKLPVKWMALESLQT  1257

         ************************************************************

S: 181   fkqfttksdvwsfGvllwelltrgatpysevasfdltlylkegrrlrkpeycpdklydvm  240

         +k fttksdvwsfGvllwel+trga+py++v++fd+t+yl +grrl +peycpd ly+vm

Q: 1258  QK-FTTKSDVWSFGVLLWELMTRGAPPYPDVNTFDITVYLLQGRRLLQPEYCPDPLYEVM  1316

         **.*********************************************************

S: 241   lkcwkakpaerpqfsdlvsiis  262

         lkcw++k++ rp+fs+lvs+is

Q: 1317  LKCWHPKAELRPSFSELVSRIS  1338

         ********************98

Domain Sequence
(FASTA)

VHFNEVIGRG HFGCVYHGTL LDNDDKKIHC AVKSLNRITD IGEVSQFLTE GIIMKDFSHP 60

NVLSLLGICL RSEGSPLVVL PYMKHGDLRN FIRNETHNPT VKDLIGFGLQ VAKGMKYLAS 120

KKFVHRDLAA RNCMLDEKFT VKVADFGLAR DMYDKEYYSV HNKTGAKLPV KWMALESLQT 180

QKFTTKSDVW SFGVLLWELM TRGAPPYPDV NTFDITVYLL QGRRLLQPEY CPDPLYEVML 240

KCWHPKAELR PSFSELVSRI S 261

Keyword

ATP-binding; Complete proteome; Disulfide bond; Glycoprotein; Kinase; Membrane; Nucleotide-binding; Phosphoprotein; Proto-oncogene; Receptor; Reference proteome; Repeat; Signal; Transferase; Transmembrane; Transmembrane helix; Tyrosine-protein kinase; Ubl conjugation.

Sequence Source

Ensembl

Orthology

Ortholog group

Ailuropoda melanoleuca"; ?>Anolis carolinensis"; ?>Bos taurus"; ?>Caenorhabditis elegans"; ?>Callithrix jacchus"; ?>Canis familiaris"; ?>Cavia porcellus"; ?>Ciona intestinalis"; ?>Ciona savignyi"; ?>Danio rerio"; ?>Felis catus"; ?>Gallus gallus"; ?>Gasterosteus aculeatus"; ?>Gorilla gorilla"; ?>Homo sapiens"; ?>Ictidomys tridecemlineatus"; ?>Latimeria chalumnae"; ?>Loxodonta africana"; ?>Macaca mulatta"; ?>Meleagris gallopavo"; ?>Monodelphis domestica"; ?>Mus musculus"; ?>Mustela putorius furo"; ?>Myotis lucifugus"; ?>Nomascus leucogenys"; ?>Ochotona princeps"; ?>Oreochromis niloticus"; ?>Ornithorhynchus anatinus"; ?>Oryctolagus cuniculus"; ?>Oryzias latipes"; ?>Otolemur garnettii"; ?>Pan troglodytes"; ?>Pelodiscus sinensis"; ?>Petromyzon marinus"; ?>Pongo abelii"; ?>Rattus norvegicus"; ?>Sarcophilus harrisii"; ?>Sus scrofa"; ?>Taeniopygia guttata"; ?>Takifugu rubripes"; ?>Tetraodon nigroviridis"; ?>Vicugna pacos"; ?>Xenopus tropicalis"; ?>Xiphophorus maculatus"; ?>

Gene Ontology

GO:0009925	; C:basal plasma membrane
GO:0016021	; C:integral to membrane
GO:0005524	; F:ATP binding
GO:0005008	; F:hepatocyte growth factor-activated receptor activity
GO:0000187	; P:activation of MAPK activity
GO:0030534	; P:adult behavior
GO:0007420	; P:brain development
GO:0048754	; P:branching morphogenesis of a tube
GO:0042593	; P:glucose homeostasis
GO:0001889	; P:liver development
GO:0014812	; P:muscle cell migration
GO:0051450	; P:myoblast proliferation
GO:0014902	; P:myotube differentiation
GO:0001890	; P:placenta development
GO:0010828	; P:positive regulation of glucose transport
GO:0046777	; P:protein autophosphorylation
GO:0060665	; P:regulation of branching involved in salivary gland morphogenesis by mesenchymal-epithelial signaling

KEGG

ecb:100056013

;

InterPros

IPR013783	; Ig-like_fold.
IPR014756	; Ig_E-set.
IPR002909	; IPT_TIG_rcpt.
IPR011009	; Kinase-like_dom.
IPR003659	; Plexin-like.
IPR016201	; Plexin-like_fold.
IPR002165	; Plexin_repeat.
IPR000719	; Prot_kinase_cat_dom.
IPR017441	; Protein_kinase_ATP_BS.
IPR001627	; Semaphorin/CD100_Ag.
IPR001245	; Ser-Thr/Tyr_kinase_cat_dom.
IPR008266	; Tyr_kinase_AS.
IPR020635	; Tyr_kinase_cat_dom.
IPR016244	; Tyr_kinase_HGF/MSP_rcpt.
IPR015943	; WD40/YVTN_repeat-like_dom.

Pfam

PF07714	; Pkinase_Tyr; 1.
PF01437	; PSI; 1.
PF01403	; Sema; 1.
PF01833	; TIG; 3.

SMARTs

SM00429	; IPT; 4.
SM00423	; PSI; 1.
SM00630	; Sema; 1.
SM00219	; TyrKc; 1.

Prosites

PS00107	; PROTEIN_KINASE_ATP; 1.
PS50011	; PROTEIN_KINASE_DOM; 1.
PS00109	; PROTEIN_KINASE_TYR; 1.
PS51004	; SEMA; 1.

Prints

PR00109

; TYRKINASE.

Created Date

20-Feb-2013