EKS-FEC-00386

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EKS-FEC-00386

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
TK/PDGFR	602.3	3.0E-181	590	925	336

Status

Reviewed

Ensembl Protein

ENSFCAP00000002867

UniProt Accession

Q28889;

Protein Name

Mast/stem cell growth factor receptor Kit

Protein Synonyms/Alias

SCFR; Proto-oncogene c-Kit; Tyrosine-protein kinase Kit;

Gene Name

KIT

Gene Synonyms/Alias

KIT;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
ENSFCAG00000003112	ENSFCAP00000002867	ENSFCAT00000003113

Organism

Felis catus

Functional Description

Tyrosine-protein kinase that acts as cell-surfacereceptor for the cytokine KITLG/SCF and plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. In response to KITLG/SCF binding, KIT can activate several signaling pathways. Phosphorylates PIK3R1, PLCG1, SH2B2/APS and CBL. Activates the AKT1 signaling pathway by phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase. Activated KIT also transmits signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. Promotes activation of STAT family members STAT1, STAT3, STAT5A and STAT5B. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. KIT signaling is modulated by protein phosphatases, and by rapid internalization and degradation of the receptor. Activated KIT promotes phosphorylation of the protein phosphatases PTPN6/SHP-1 and PTPRU, and of the transcription factors STAT1, STAT3, STAT5A and STAT5B. Promotes phosphorylation of PIK3R1, CBL, CRK (isoform Crk-II), LYN, MAPK1/ERK2 and/or MAPK3/ERK1, PLCG1, SRC and SHC1 (By similarity).

Protein Length

978

Protein Sequence
(FASTA)

MRGARGAWDF LCVLLLLLRV QTGSSQPSAS PGEWSLPSIH PATSELIVSA GDEIRLLCTD 60

PGFVKWTFET LGQSSEITHN EWITEKAEAT NTGNYTCTNG GGLSSSIYVF VRDPAKLFLV 120

DLPLYGKEDH DTLVRCPLTD PEVTNYSLRG CEGKPLPKDL TFVTDPKAGI TIRNVKREYH 180

RLCLHCSADR KGKSVLSKKF TLKVRAAIRA VPVVSVSKAS HLLREGEEFS VMCLIKDVSS 240

SVDSMWIKEN SPQTNAQPQS NSWHQGDFNF VRQERLTISS ARVNDSGVFM CYANNTFGSA 300

NVTTTLEVVA KGFINIFPMM NTTIFVNDGE NVDLIVEYEA YPKPEHQRWV YMNRTLTDKW 360

EDYPKSDNES NIRYVSELHL TRLKGNEGGT YTFQVSNSDV NSSVTLNVYV NTKPEILTHE 420

SLVSGILQCL VAGFPEPTVD WYFCPGAEQR CPVPVGPLDV QMQNSSVSPS GKLVVQSSID 480

YSAFKHNGTV ECRASNNVGK TSAFFNFAFK GNSKEQMHPH TLFTPLLIGF VIAAGMMCII 540

VMILTYKYLQ KPMYEVQWKV VEEINGNNYV YIDPTQLPYD HKWEFPRNRL SFGKTLGAGA 600

FGKVVEATAY GLIKSDAAMT VAVKMLKPSA HLTEREALMS ELKVLSYLGN HMNIVNLLGA 660

CTVGGPTLVI TEYCCYGDLL NFLRRKRDSF ICSKQEDHAE VALYKNLLQS KESSCNDSTN 720

EYMDMKPGVS YVVPTKADKR RSARIGSYIE RDVTPAIMED DELALDLEDL LSFSYQVAKG 780

MAFLASKNCI HRDLAARNIL LTHGRITKIC DFGLARDIKN DSNYVVKGNA RLPVKWMAPE 840

SIFNCVYTFE SDVWSYGIFL WELFSLGSSP YPGMPVDSKF YKMIKEGFRM LSPEHAPAEM 900

YDIMKTCWDA DPLKRPTFKQ IVQLIEKQIS DSTNHIYSNL ANCSPNRERP TAVDHCVRIN 960

SVGSSTSSTQ PLLVHEDA 978

Nucleotide Sequence
(FASTA)

ATGAGAGGCG CTCGCGGCGC CTGGGATTTT CTCTGCGTCC TGCTCCTCCT GCTCCGCGTC 60

CAGACAGGCT CTTCTCAACC ATCTGCGAGT CCGGGGGAAT GGTCTCTACC GTCCATCCAT 120

CCAGCAACGT CAGAGTTAAT AGTCAGTGCT GGCGACGAGA TTAGGCTGTT GTGCACCGAT 180

CCAGGGTTTG TCAAATGGAC TTTTGAGACC CTGGGCCAGT CGAGTGAGAT CACACACAAT 240

GAATGGATCA CGGAGAAAGC AGAGGCCACC AACACGGGCA ATTACACGTG CACCAACGGA 300

GGCGGCTTAA GCAGTTCCAT TTACGTGTTT GTTAGAGATC CCGCAAAGCT TTTCCTCGTT 360

GACCTTCCCT TGTATGGGAA AGAAGACCAT GATACGCTGG TCCGCTGTCC TCTGACAGAC 420

CCAGAGGTGA CCAATTACTC CCTCCGGGGG TGCGAGGGGA AGCCTCTTCC CAAGGACTTG 480

ACTTTCGTCA CCGATCCCAA GGCTGGCATC ACAATCAGGA ATGTGAAGCG CGAGTACCAT 540

CGGCTGTGCC TGCATTGCTC TGCGGACCGG AAGGGCAAGT CCGTGCTGTC GAAGAAATTC 600

ACCCTGAAAG TGAGGGCAGC CATCAGAGCT GTCCCAGTTG TGTCTGTGTC CAAAGCAAGC 660

CATCTTCTTA GGGAAGGGGA AGAATTCTCC GTGATGTGCT TGATAAAAGA TGTGTCTAGT 720

TCCGTGGACT CGATGTGGAT CAAGGAAAAC AGCCCGCAAA CTAATGCACA GCCACAGAGT 780

AATAGCTGGC ACCAAGGTGA CTTCAACTTT GTACGTCAGG AAAGGTTGAC GATCAGCTCA 840

GCAAGAGTTA ACGATTCTGG AGTGTTCATG TGTTATGCCA ATAATACTTT TGGATCGGCA 900

AATGTCACAA CAACCTTGGA AGTCGTAGCT AAAGGATTCA TTAACATCTT CCCTATGATG 960

AATACCACAA TATTCGTGAA TGATGGCGAG AATGTGGATC TGATTGTCGA ATATGAGGCA 1020

TATCCCAAGC CTGAGCACCA ACGGTGGGTC TATATGAACA GAACCCTCAC TGATAAATGG 1080

GAAGATTATC CCAAGTCTGA CAACGAAAGT AATATCAGAT ATGTGAGTGA ACTTCATCTA 1140

ACCAGATTAA AAGGGAACGA AGGAGGCACT TACACGTTTC AAGTGTCCAA TTCCGATGTC 1200

AATTCTTCCG TGACATTGAA TGTTTACGTG AACACAAAAC CAGAAATCCT GACTCATGAA 1260

AGTCTCGTGA GTGGCATACT ACAGTGTTTG GTAGCCGGAT TCCCGGAGCC CACAGTAGAT 1320

TGGTATTTTT GTCCAGGAGC TGAGCAGAGA TGTCCTGTTC CTGTTGGGCC ACTGGATGTG 1380

CAGATGCAAA ACTCATCTGT ATCGCCGTCT GGAAAACTAG TGGTTCAAAG TTCCATCGAT 1440

TACAGTGCTT TCAAACACAA TGGCACAGTC GAGTGTAGGG CTTCCAACAA TGTGGGCAAG 1500

ACTTCTGCCT TTTTTAACTT TGCATTTAAA GGTAACAGCA AAGAACAAAT GCATCCTCAC 1560

ACCCTGTTCA CACCTTTGCT GATTGGTTTT GTGATCGCAG CTGGTATGAT GTGTATCATC 1620

GTGATGATAC TTACCTACAA ATATTTACAG AAACCCATGT ATGAAGTGCA GTGGAAGGTT 1680

GTCGAGGAGA TAAATGGAAA CAATTATGTT TACATAGACC CAACACAGCT TCCTTACGAT 1740

CACAAATGGG AGTTTCCCAG AAACAGGCTG AGTTTTGGGA AAACCTTGGG TGCCGGTGCC 1800

TTCGGGAAGG TGGTTGAGGC CACTGCATAT GGCCTGATTA AGTCGGATGC AGCCATGACC 1860

GTTGCTGTTA AGATGCTCAA ACCAAGTGCC CATTTAACCG AACGAGAAGC CCTCATGTCC 1920

GAGCTCAAAG TCTTGAGTTA CCTCGGCAAT CACATGAATA TTGTGAATCT TCTCGGAGCA 1980

TGCACCGTTG GAGGGCCCAC CCTGGTCATT ACTGAGTATT GTTGCTATGG TGATCTTTTG 2040

AATTTTTTGA GAAGAAAACG TGATTCATTT ATTTGCTCAA AGCAGGAAGA TCATGCAGAA 2100

GTGGCACTTT ATAAGAACCT TCTGCAGTCA AAGGAGTCTT CCTGCAACGA TAGTACTAAT 2160

GAATACATGG ACATGAAACC CGGAGTTTCT TATGTTGTGC CAACCAAGGC AGACAAAAGG 2220

AGATCTGCCA GGATAGGCTC ATACATAGAA AGAGATGTGA CTCCCGCCAT CATGGAGGAC 2280

GATGAGTTGG CTCTAGACCT GGAGGACTTG CTGAGCTTCT CGTACCAGGT GGCCAAGGGC 2340

ATGGCATTCC TCGCCTCGAA GAATTGTATT CACAGAGACT TGGCTGCTAG AAATATCCTC 2400

CTTACTCATG GTCGAATCAC AAAGATTTGT GATTTTGGTC TAGCCAGAGA CATCAAGAAT 2460

GATTCTAATT ATGTGGTCAA AGGAAACGCT CGGCTACCTG TGAAGTGGAT GGCACCTGAA 2520

AGCATTTTCA ACTGTGTGTA CACATTTGAA AGTGATGTCT GGTCCTATGG GATTTTTCTG 2580

TGGGAGCTGT TCTCTTTAGG AAGCAGCCCC TACCCTGGAA TGCCAGTCGA CTCTAAGTTC 2640

TACAAGATGA TCAAGGAAGG GTTCCGAATG CTCAGCCCTG AGCACGCACC TGCTGAAATG 2700

TATGACATAA TGAAGACTTG CTGGGATGCT GATCCCCTGA AAAGGCCAAC ATTCAAGCAG 2760

ATCGTGCAGC TAATCGAGAA GCAGATTTCA GATAGCACCA ATCATATTTA TTCCAACTTA 2820

GCAAACTGCA GCCCGAACCG GGAGCGCCCC ACAGCAGTGG ACCATTGTGT GCGGATCAAT 2880

TCTGTGGGCA GCAGCACATC TTCTACCCAA CCTCTGCTGG TCCACGAGGA TGCCTGA 2937

Domain Profile

S: 1     lelGkvlGsGafGkvveatayGlsksdavlkvavkmlkstarssekealmselkilshlG  60

         l++Gk+lG+GafGkvveatayGl+ksda+++vavkmlk++a+ +e+ealmselk+ls+lG

Q: 590   LSFGKTLGAGAFGKVVEATAYGLIKSDAAMTVAVKMLKPSAHLTEREALMSELKVLSYLG  649

         79**********************************************************

S: 61    ehenivnllGactkgGpiyviteyccyGdllnylrrkresflkkslekkkekeeeldska  120

         +h+nivnllGact+gGp++viteyccyGdlln+lrrkr+sf+   ++k+++++e + +k+

Q: 650   NHMNIVNLLGACTVGGPTLVITEYCCYGDLLNFLRRKRDSFI---CSKQEDHAEVALYKN  706

         ******************************************...999999*99999999

S: 121   lladkslrsksslssesseeyvdlkkdvsdvssskadklasekkksalerevkaaleeed  180

         l     l+sk+s++ +s++ey+d+k++vs+v ++kadk++s+++ s++er+v++a++e+d

Q: 707   L-----LQSKESSCNDSTNEYMDMKPGVSYVVPTKADKRRSARIGSYIERDVTPAIMEDD  761

         9.....9*****************************************************

S: 181   sevleledllsfsyqvakGmeflaskncvhrdlaarnvllteGkvvkicdfGlardimkd  240

         + +l+ledllsfsyqvakGm+flasknc+hrdlaarn+llt+G+++kicdfGlardi++d

Q: 762   ELALDLEDLLSFSYQVAKGMAFLASKNCIHRDLAARNILLTHGRITKICDFGLARDIKND  821

         ************************************************************

S: 241   snyvvkGnarlpvkwmapesifdslytvksdvwsyGillweifslGstpypgllvdskfy  300

         snyvvkGnarlpvkwmapesif+++yt++sdvwsyGi+lwe+fslGs+pypg++vdskfy

Q: 822   SNYVVKGNARLPVKWMAPESIFNCVYTFESDVWSYGIFLWELFSLGSSPYPGMPVDSKFY  881

         ************************************************************

S: 301   klikeGyrmaqpayateeiyeimqkcweaepekrpsfkqlvell  344

         k+ikeG+rm +p++a++e+y+im++cw+a+p krp+fkq+v+l+

Q: 882   KMIKEGFRMLSPEHAPAEMYDIMKTCWDADPLKRPTFKQIVQLI  925

         *****************************************996

Domain Sequence
(FASTA)

LSFGKTLGAG AFGKVVEATA YGLIKSDAAM TVAVKMLKPS AHLTEREALM SELKVLSYLG 60

NHMNIVNLLG ACTVGGPTLV ITEYCCYGDL LNFLRRKRDS FICSKQEDHA EVALYKNLLQ 120

SKESSCNDST NEYMDMKPGV SYVVPTKADK RRSARIGSYI ERDVTPAIME DDELALDLED 180

LLSFSYQVAK GMAFLASKNC IHRDLAARNI LLTHGRITKI CDFGLARDIK NDSNYVVKGN 240

ARLPVKWMAP ESIFNCVYTF ESDVWSYGIF LWELFSLGSS PYPGMPVDSK FYKMIKEGFR 300

MLSPEHAPAE MYDIMKTCWD ADPLKRPTFK QIVQLI 336

Keyword

ATP-binding; Cell membrane; Disulfide bond; Glycoprotein; Immunoglobulin domain; Kinase; Magnesium; Membrane; Metal-binding; Nucleotide-binding; Phosphoprotein; Proto-oncogene; Receptor; Repeat; Signal; Transferase; Transmembrane; Transmembrane helix; Tyrosine-protein kinase; Ubl conjugation.

Sequence Source

Ensembl

Orthology

Ortholog group

Ailuropoda melanoleuca"; ?>Anolis carolinensis"; ?>Bos taurus"; ?>Callithrix jacchus"; ?>Canis familiaris"; ?>Cavia porcellus"; ?>Danio rerio"; ?>Equus caballus"; ?>Gallus gallus"; ?>Gasterosteus aculeatus"; ?>Gorilla gorilla"; ?>Homo sapiens"; ?>Ictidomys tridecemlineatus"; ?>Latimeria chalumnae"; ?>Loxodonta africana"; ?>Macaca mulatta"; ?>Meleagris gallopavo"; ?>Monodelphis domestica"; ?>Mus musculus"; ?>Mustela putorius furo"; ?>Myotis lucifugus"; ?>Nomascus leucogenys"; ?>Oreochromis niloticus"; ?>Ornithorhynchus anatinus"; ?>Oryctolagus cuniculus"; ?>Oryzias latipes"; ?>Otolemur garnettii"; ?>Pan troglodytes"; ?>Pelodiscus sinensis"; ?>Pongo abelii"; ?>Pteropus vampyrus"; ?>Rattus norvegicus"; ?>Sarcophilus harrisii"; ?>Sus scrofa"; ?>Taeniopygia guttata"; ?>Takifugu rubripes"; ?>Tetraodon nigroviridis"; ?>Xenopus tropicalis"; ?>Xiphophorus maculatus"; ?>

Gene Ontology

GO:0016021	; C:integral to membrane
GO:0005886	; C:plasma membrane
GO:0005524	; F:ATP binding
GO:0019955	; F:cytokine binding
GO:0046872	; F:metal ion binding
GO:0004714	; F:transmembrane receptor protein tyrosine kinase activity
GO:0031532	; P:actin cytoskeleton reorganization
GO:0000187	; P:activation of MAPK activity
GO:0060326	; P:cell chemotaxis
GO:0002371	; P:dendritic cell cytokine production
GO:0050910	; P:detection of mechanical stimulus involved in sensory perception of sound
GO:0048565	; P:digestive tract development
GO:0035162	; P:embryonic hemopoiesis
GO:0030218	; P:erythrocyte differentiation
GO:0038162	; P:erythropoietin-mediated signaling pathway
GO:0038093	; P:Fc receptor signaling pathway
GO:0002327	; P:immature B cell differentiation
GO:0006954	; P:inflammatory response
GO:0038109	; P:Kit signaling pathway
GO:0030032	; P:lamellipodium assembly
GO:0032762	; P:mast cell cytokine production
GO:0043303	; P:mast cell degranulation
GO:0060374	; P:mast cell differentiation
GO:0035855	; P:megakaryocyte development
GO:0097326	; P:melanocyte adhesion
GO:0030318	; P:melanocyte differentiation
GO:0097324	; P:melanocyte migration
GO:0001541	; P:ovarian follicle development
GO:0018108	; P:peptidyl-tyrosine phosphorylation
GO:0008360	; P:regulation of cell shape
GO:0007283	; P:spermatogenesis
GO:0048863	; P:stem cell differentiation
GO:0030217	; P:T cell differentiation

KEGG

InterPros

IPR007110	; Ig-like.
IPR013783	; Ig-like_fold.
IPR013098	; Ig_I-set.
IPR003599	; Ig_sub.
IPR011009	; Kinase-like_dom.
IPR000719	; Prot_kinase_cat_dom.
IPR017441	; Protein_kinase_ATP_BS.
IPR001245	; Ser-Thr/Tyr_kinase_cat_dom.
IPR008266	; Tyr_kinase_AS.
IPR020635	; Tyr_kinase_cat_dom.
IPR016243	; Tyr_kinase_CSF1/PDGF_rcpt.
IPR001824	; Tyr_kinase_rcpt_3_CS.

Pfam

PF07679	; I-set; 1.
PF07714	; Pkinase_Tyr; 1.

SMARTs

SM00409	; IG; 3.
SM00219	; TyrKc; 1.

Prosites

PS50835	; IG_LIKE; 2.
PS00107	; PROTEIN_KINASE_ATP; 1.
PS50011	; PROTEIN_KINASE_DOM; 1.
PS00109	; PROTEIN_KINASE_TYR; 1.
PS00240	; RECEPTOR_TYR_KIN_III; 1.

Prints

Created Date

20-Feb-2013