EKS-HOS-00371

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EKS-HOS-00371

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
TK/Src	451.3	4.4E-135	271	520	250

Status

Reviewed

Ensembl Protein

ENSP00000346671

UniProt Accession

P06241; B5BU57; E1P557; H0UI48; Q16248; Q5R3A6; Q5R3A7; Q8N5D7;

Protein Name

Tyrosine-protein kinase Fyn

Protein Synonyms/Alias

Proto-oncogene Syn; Proto-oncogene c-Fyn; Src-like kinase; SLK; p59-Fyn;

Gene Name

FYN

Gene Synonyms/Alias

FYN;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
ENSG00000010810	ENSP00000348295	ENST00000356013
ENSG00000010810	ENSP00000428493	ENST00000524310
ENSG00000010810	ENSP00000429992	ENST00000523574
ENSG00000010810	ENSP00000428042	ENST00000521062
ENSG00000010810	ENSP00000429813	ENST00000518630
ENSG00000010810	ENSP00000357671	ENST00000368682
ENSG00000010810	ENSP00000440646	ENST00000538466
ENSG00000010810	ENSP00000429590	ENST00000462598
ENSG00000010810	ENSP00000428695	ENST00000518295
ENSG00000010810	ENSP00000445439	ENST00000544792
ENSG00000010810	ENSP00000229470	ENST00000229470
ENSG00000010810	ENSP00000229471	ENST00000229471
ENSG00000010810	ENSP00000428045	ENST00000523570
ENSG00000010810	ENSP00000429294	ENST00000520518
ENSG00000010810	ENSP00000430455	ENST00000487824
ENSG00000010810	ENSP00000346671	ENST00000354650
ENSG00000010810	ENSP00000427993	ENST00000462856
ENSG00000010810	ENSP00000430364	ENST00000523238
ENSG00000010810	ENSP00000429866	ENST00000517419
ENSG00000010810	ENSP00000357656	ENST00000368667
ENSG00000010810	ENSP00000357667	ENST00000368678
ENSG00000010810	ENSP00000428983	ENST00000484067

Organism

Homo sapiens

Functional Description

Non-receptor tyrosine-protein kinase that plays a rolein many biological processes including regulation of cell growth and survival, cell adhesion, integrin-mediated signaling, cytoskeletal remodeling, cell motility, immune response and axon guidance. Inactive FYN is phosphorylated on its C-terminal tail within the catalytic domain. Following activation by PKA, the protein subsequently associates with PTK2/FAK1, allowing PTK2/FAK1 phosphorylation, activation and targeting to focal adhesions. Involved in the regulation of cell adhesion and motility through phosphorylation of CTNNB1 (beta-catenin) and CTNND1 (delta- catenin). Regulates cytoskeletal remodeling by phosphorylating several proteins including the actin regulator WAS and the microtubule-associated proteins MAP2 and MAPT. Promotes cell survival by phosphorylating AGAP2/PIKE-A and preventing its apoptotic cleavage. Participates in signal transduction pathways that regulate the integrity of the glomerular slit diaphragm (an essential part of the glomerular filter of the kidney) by phosphorylating several slit diaphragm components including NPHS1, KIRREL and TRPC6. Plays a role in neural processes by phosphorylating DPYSL2, a multifunctional adapter protein within the central nervous system, ARHGAP32, a regulator for Rho family GTPases implicated in various neural functions, and SNCA, a small pre-synaptic protein. Participates in the downstream signaling pathways that lead to T-cell differentiation and proliferation following T-cell receptor (TCR) stimulation. Also participates in negative feedback regulation of TCR signaling through phosphorylation of PAG1, thereby promoting interaction between PAG1 and CSK and recruitment of CSK to lipid rafts. CSK maintains LCK and FYN in an inactive form. Promotes CD28-induced phosphorylation of VAV1.

Protein Length

537

Protein Sequence
(FASTA)

MGCVQCKDKE ATKLTEERDG SLNQSSGYRY GTDPTPQHYP SFGVTSIPNY NNFHAAGGQG 60

LTVFGGVNSS SHTGTLRTRG GTGVTLFVAL YDYEARTEDD LSFHKGEKFQ ILNSSEGDWW 120

EARSLTTGET GYIPSNYVAP VDSIQAEEWY FGKLGRKDAE RQLLSFGNPR GTFLIRESET 180

TKGAYSLSIR DWDDMKGDHV KHYKIRKLDN GGYYITTRAQ FETLQQLVQH YSERAAGLCC 240

RLVVPCHKGM PRLTDLSVKT KDVWEIPRES LQLIKRLGNG QFGEVWMGTW NGNTKVAIKT 300

LKPGTMSPES FLEEAQIMKK LKHDKLVQLY AVVSEEPIYI VTEYMNKGSL LDFLKDGEGR 360

ALKLPNLVDM AAQVAAGMAY IERMNYIHRD LRSANILVGN GLICKIADFG LARLIEDNEY 420

TARQGAKFPI KWTAPEAALY GRFTIKSDVW SFGILLTELV TKGRVPYPGM NNREVLEQVE 480

RGYRMPCPQD CPISLHELMI HCWKKDPEER PTFEYLQSFL EDYFTATEPQ YQPGENL 537

Nucleotide Sequence
(FASTA)

ATGGGCTGTG TGCAATGTAA GGATAAAGAA GCAACAAAAC TGACGGAGGA GAGGGACGGC 60

AGCCTGAACC AGAGCTCTGG GTACCGCTAT GGCACAGACC CCACCCCTCA GCACTACCCC 120

AGCTTCGGTG TGACCTCCAT CCCCAACTAC AACAACTTCC ACGCAGCCGG GGGCCAAGGA 180

CTCACCGTCT TTGGAGGTGT GAACTCTTCG TCTCATACGG GGACCTTGCG TACGAGAGGA 240

GGAACAGGAG TGACACTCTT TGTGGCCCTT TATGACTATG AAGCACGGAC AGAAGATGAC 300

CTGAGTTTTC ACAAAGGAGA AAAATTTCAA ATATTGAACA GCTCGGAAGG AGATTGGTGG 360

GAAGCCCGCT CCTTGACAAC TGGAGAGACA GGTTACATTC CCAGCAATTA TGTGGCTCCA 420

GTTGACTCTA TCCAGGCAGA AGAGTGGTAC TTTGGAAAAC TTGGCCGAAA AGATGCTGAG 480

CGACAGCTAT TGTCCTTTGG AAACCCAAGA GGTACCTTTC TTATCCGCGA GAGTGAAACC 540

ACCAAAGGTG CCTATTCACT TTCTATCCGT GATTGGGATG ATATGAAAGG AGACCATGTC 600

AAACATTATA AAATTCGCAA ACTTGACAAT GGTGGATACT ACATTACCAC CCGGGCCCAG 660

TTTGAAACAC TTCAGCAGCT TGTACAACAT TACTCAGAGA GAGCTGCAGG TCTCTGCTGC 720

CGCCTAGTAG TTCCCTGTCA CAAAGGGATG CCAAGGCTTA CCGATCTGTC TGTCAAAACC 780

AAAGATGTCT GGGAAATCCC TCGAGAATCC CTGCAGTTGA TCAAGAGACT GGGAAATGGG 840

CAGTTTGGGG AAGTATGGAT GGGTACCTGG AATGGAAACA CAAAAGTAGC CATAAAGACT 900

CTTAAACCAG GCACAATGTC CCCCGAATCA TTCCTTGAGG AAGCGCAGAT CATGAAGAAG 960

CTGAAGCACG ACAAGCTGGT CCAGCTCTAT GCAGTGGTGT CTGAGGAGCC CATCTACATC 1020

GTCACCGAGT ATATGAACAA AGGAAGTTTA CTGGATTTCT TAAAAGATGG AGAAGGAAGA 1080

GCTCTGAAAT TACCAAATCT TGTGGACATG GCAGCACAGG TGGCTGCAGG AATGGCTTAC 1140

ATCGAGCGCA TGAATTATAT CCATAGAGAT CTGCGATCAG CAAACATTCT AGTGGGGAAT 1200

GGACTCATAT GCAAGATTGC TGACTTCGGA TTGGCCCGAT TGATAGAAGA CAATGAGTAC 1260

ACAGCAAGAC AAGGTGCAAA GTTCCCCATC AAGTGGACGG CCCCCGAGGC AGCCCTGTAC 1320

GGGAGGTTCA CAATCAAGTC TGACGTGTGG TCTTTTGGAA TCTTACTCAC AGAGCTGGTC 1380

ACCAAAGGAA GAGTGCCATA CCCAGGCATG AACAACCGGG AGGTGCTGGA GCAGGTGGAG 1440

CGAGGCTACA GGATGCCCTG CCCGCAGGAC TGCCCCATCT CTCTGCATGA GCTCATGATC 1500

CACTGCTGGA AAAAGGACCC TGAAGAACGC CCCACTTTTG AGTACTTGCA GAGCTTCCTG 1560

GAAGACTACT TTACCGCGAC AGAGCCCCAG TACCAACCTG GTGAAAACCT GTAA 1614

Domain Profile

S: 1     lklvkklGeGqfGevwlgkwkgsvkvavktlkegtlspeafleeaqilkklrheklvkly  60

         l+l+k+lG+GqfGevw+g+w+g++kva+ktlk+gt+spe+fleeaqi+kkl+h+klv+ly

Q: 271   LQLIKRLGNGQFGEVWMGTWNGNTKVAIKTLKPGTMSPESFLEEAQIMKKLKHDKLVQLY  330

         689*********************************************************

S: 61    avvseeePiyivtelmakGslldflkeeegkklklpklvdlaaqvaeGmayleeknlihr  120

         avvse ePiyivte+m+kGslldflk+ eg++lklp+lvd+aaqva+Gmay+e++n+ihr

Q: 331   AVVSE-EPIYIVTEYMNKGSLLDFLKDGEGRALKLPNLVDMAAQVAAGMAYIERMNYIHR  389

         ****9.******************************************************

S: 121   dlaarnvlvgeslvvkvadfGlarlieddeytakegaklPikWtaPeaiklgkftiksdv  180

         dl+++n+lvg+ l++k+adfGlarlied+eyta++gak+PikWtaPea+ +g+ftiksdv

Q: 390   DLRSANILVGNGLICKIADFGLARLIEDNEYTARQGAKFPIKWTAPEAALYGRFTIKSDV  449

         ************************************************************

S: 181   WsfGillteivtkGkvPypGmtneevleqvergyrlprpeecpeelyelllecwkkdpee  240

         WsfGillte+vtkG+vPypGm+n+evleqvergyr+p+p++cp +l+el+++cwkkdpee

Q: 450   WSFGILLTELVTKGRVPYPGMNNREVLEQVERGYRMPCPQDCPISLHELMIHCWKKDPEE  509

         ************************************************************

S: 241   rPtfetlqevl  251

         rPtfe+lq++l

Q: 510   RPTFEYLQSFL  520

         ********986

Domain Sequence
(FASTA)

LQLIKRLGNG QFGEVWMGTW NGNTKVAIKT LKPGTMSPES FLEEAQIMKK LKHDKLVQLY 60

AVVSEEPIYI VTEYMNKGSL LDFLKDGEGR ALKLPNLVDM AAQVAAGMAY IERMNYIHRD 120

LRSANILVGN GLICKIADFG LARLIEDNEY TARQGAKFPI KWTAPEAALY GRFTIKSDVW 180

SFGILLTELV TKGRVPYPGM NNREVLEQVE RGYRMPCPQD CPISLHELMI HCWKKDPEER 240

PTFEYLQSFL 250

Keyword

3D-structure; Adaptive immunity; Alternative splicing; ATP-binding; Cell membrane; Complete proteome; Cytoplasm; Developmental protein; Host-virus interaction; Immunity; Kinase; Lipoprotein; Manganese; Membrane; Metal-binding; Myristate; Nucleotide-binding; Nucleus; Palmitate; Phosphoprotein; Polymorphism; Proto-oncogene; Reference proteome; SH2 domain; SH3 domain; Transferase; Tyrosine-protein kinase.

Sequence Source

Ensembl

Orthology

Ortholog group

Ailuropoda melanoleuca"; ?>Anolis carolinensis"; ?>Bos taurus"; ?>Caenorhabditis elegans"; ?>Callithrix jacchus"; ?>Canis familiaris"; ?>Cavia porcellus"; ?>Choloepus hoffmanni"; ?>Danio rerio"; ?>Dasypus novemcinctus"; ?>Drosophila melanogaster"; ?>Equus caballus"; ?>Felis catus"; ?>Gadus morhua"; ?>Gallus gallus"; ?>Gasterosteus aculeatus"; ?>Gorilla gorilla"; ?>Ictidomys tridecemlineatus"; ?>Latimeria chalumnae"; ?>Loxodonta africana"; ?>Macaca mulatta"; ?>Meleagris gallopavo"; ?>Monodelphis domestica"; ?>Mus musculus"; ?>Mustela putorius furo"; ?>Myotis lucifugus"; ?>Nomascus leucogenys"; ?>Oreochromis niloticus"; ?>Ornithorhynchus anatinus"; ?>Oryctolagus cuniculus"; ?>Oryzias latipes"; ?>Otolemur garnettii"; ?>Pan troglodytes"; ?>Pelodiscus sinensis"; ?>Pongo abelii"; ?>Procavia capensis"; ?>Pteropus vampyrus"; ?>Rattus norvegicus"; ?>Sarcophilus harrisii"; ?>Sus scrofa"; ?>Taeniopygia guttata"; ?>Takifugu rubripes"; ?>Tetraodon nigroviridis"; ?>Tupaia belangeri"; ?>Tursiops truncatus"; ?>Vicugna pacos"; ?>Xenopus tropicalis"; ?>Xiphophorus maculatus"; ?>

Gene Ontology

GO:0005829	; C:cytosol
GO:0005768	; C:endosome
GO:0005739	; C:mitochondrion
GO:0005886	; C:plasma membrane
GO:0005524	; F:ATP binding
GO:0046872	; F:metal ion binding
GO:0004715	; F:non-membrane spanning protein tyrosine kinase activity
GO:0050798	; P:activated T cell proliferation
GO:0007411	; P:axon guidance
GO:0006816	; P:calcium ion transport
GO:0071363	; P:cellular response to growth factor stimulus
GO:0071375	; P:cellular response to peptide hormone stimulus
GO:0048813	; P:dendrite morphogenesis
GO:0050966	; P:detection of mechanical stimulus involved in sensory perception of pain
GO:0007631	; P:feeding behavior
GO:0030900	; P:forebrain development
GO:0007243	; P:intracellular protein kinase cascade
GO:0007612	; P:learning
GO:0050900	; P:leukocyte migration
GO:0010629	; P:negative regulation of gene expression
GO:0042177	; P:negative regulation of protein catabolic process
GO:0001764	; P:neuron migration
GO:0018108	; P:peptidyl-tyrosine phosphorylation
GO:0030168	; P:platelet activation
GO:0046777	; P:protein autophosphorylation
GO:0008360	; P:regulation of cell shape
GO:0050690	; P:regulation of defense response to virus by virus
GO:0042493	; P:response to drug
GO:0045471	; P:response to ethanol
GO:0031295	; P:T cell costimulation
GO:0050852	; P:T cell receptor signaling pathway
GO:0016032	; P:viral reproduction
GO:0019048	; P:virus-host interaction

KEGG

hsa:2534

;

InterPros

IPR011009	; Kinase-like_dom.
IPR000719	; Prot_kinase_cat_dom.
IPR017441	; Protein_kinase_ATP_BS.
IPR001245	; Ser-Thr/Tyr_kinase_cat_dom.
IPR000980	; SH2.
IPR001452	; SH3_domain.
IPR008266	; Tyr_kinase_AS.
IPR020635	; Tyr_kinase_cat_dom.

Pfam

PF07714	; Pkinase_Tyr; 1.
PF00017	; SH2; 1.
PF00018	; SH3_1; 1.

SMARTs

SM00252	; SH2; 1.
SM00326	; SH3; 1.
SM00219	; TyrKc; 1.

Prosites

PS00107	; PROTEIN_KINASE_ATP; 1.
PS50011	; PROTEIN_KINASE_DOM; 1.
PS00109	; PROTEIN_KINASE_TYR; 1.
PS50001	; SH2; 1.
PS50002	; SH3; 1.

Prints

PR00401	; SH2DOMAIN.
PR00452	; SH3DOMAIN.
PR00109	; TYRKINASE.

Created Date

20-Feb-2013