| Tag | Content |
|---|
| EKPD ID | EKS-HOS-00472 |
| Classification | | Group/Family | Score | E-Value | Start | End | Domain Length |
|---|
| CMGC/MAPK | 420.4 | 1.6E-125 | 26 | 321 | 296 |
|
| Status | Reviewed |
| Ensembl Protein | ENSP00000345524 |
| UniProt Accession | P45984; A8K0S3; B5BU66; B5M0B4; D3DWQ8; D3DWQ9; Q15708; Q15710; Q15711; Q8N5C5; |
| Protein Name | Mitogen-activated protein kinase 9 |
| Protein Synonyms/Alias | MAP kinase 9; MAPK 9; JNK-55; Stress-activated protein kinase 1a; SAPK1a; Stress-activated protein kinase JNK2; c-Jun N-terminal kinase 2; |
| Gene Name | MAPK9 |
| Gene Synonyms/Alias | MAPK9; JNK2, PRKM9, SAPK1A; |
| Ensembl Information | |
| Organism | Homo sapiens |
| Functional Description | Serine/threonine-protein kinase involved in variousprocesses such as cell proliferation, differentiation, migration, transformation and programmed cell death. Extracellular stimuli such as proinflammatory cytokines or physical stress stimulate the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. In this cascade, two dual specificity kinases MAP2K4/MKK4 and MAP2K7/MKK7 phosphorylate and activate MAPK9/JNK2. In turn, MAPK9/JNK2 phosphorylates a number of transcription factors, primarily components of AP-1 such as JUN and ATF2 and thus regulates AP-1 transcriptional activity. In response to oxidative or ribotoxic stresses, inhibits rRNA synthesis by phosphorylating and inactivating the RNA polymerase 1-specific transcription initiation factor RRN3. Promotes stressed cell apoptosis by phosphorylating key regulatory factors including TP53 and YAP1. In T-cells, MAPK8 and MAPK9 are required for polarized differentiation of T-helper cells into Th1 cells. Upon T-cell receptor (TCR) stimulation, is activated by CARMA1, BCL10, MAP2K7 and MAP3K7/TAK1 to regulate JUN protein levels. Plays an important role in the osmotic stress-induced epithelial tight-junctions disruption. When activated, promotes beta-catenin/CTNNB1 degradation and inhibits the canonical Wnt signaling pathway. Participates also in neurite growth in spiral ganglion neurons. MAPK9 isoforms display different binding patterns:alpha-1 and alpha-2 preferentially bind to JUN, whereas beta-1 and beta-2 bind to ATF2. However, there is no correlation between binding and phosphorylation, which is achieved at about the same efficiency by all isoforms. JUNB is not a substrate for JNK2 alpha-2, and JUND binds only weakly to it. |
| Protein Length | 382 |
Protein Sequence
(FASTA) | | MSDSKCDSQF YSVQVADSTF TVLKRYQQLK PIGSGAQGIV CAAFDTVLGI NVAVKKLSRP 60 | | FQNQTHAKRA YRELVLLKCV NHKNIISLLN VFTPQKTLEE FQDVYLVMEL MDANLCQVIH 120 | | MELDHERMSY LLYQMLCGIK HLHSAGIIHR DLKPSNIVVK SDCTLKILDF GLARTACTNF 180 | | MMTPYVVTRY YRAPEVILGM GYKENVDIWS VGCIMAEMVL HKVLFPGRDY IDQWNKVIEQ 240 | | LGTPSAEFMK KLQPTVRNYV ENRPKYPGIK FEELFPDWIF PSESERDKIK TSQARDLLSK 300 | | MLVIDPDKRI SVDEALRHPY ITVWYDPAEA EAPPPQIYDA QLEEREHAIE EWKELIYKEV 360 | | MDWEERSKNG VVKDQPSAQM QQ 382 |
|
Nucleotide Sequence
(FASTA) | | ATGAGCGACA GTAAATGTGA CAGTCAGTTT TATAGTGTGC AAGTGGCAGA CTCAACCTTC 60 | | ACTGTCCTAA AACGTTACCA GCAGCTGAAA CCAATTGGCT CTGGGGCCCA AGGGATTGTT 120 | | TGTGCTGCAT TTGATACAGT TCTTGGGATA AATGTTGCAG TCAAGAAACT AAGCCGTCCT 180 | | TTTCAGAACC AAACTCATGC AAAGAGAGCT TATCGTGAAC TTGTCCTCTT AAAATGTGTC 240 | | AATCATAAAA ATATAATTAG TTTGTTAAAT GTGTTTACAC CACAAAAAAC TCTAGAAGAA 300 | | TTTCAAGATG TGTATTTGGT TATGGAATTA ATGGATGCTA ACTTATGTCA GGTTATTCAC 360 | | ATGGAGCTGG ATCATGAAAG AATGTCCTAC CTTCTTTACC AGATGCTTTG TGGTATTAAA 420 | | CATCTGCATT CAGCTGGTAT AATTCATAGA GATTTGAAGC CTAGCAACAT TGTTGTGAAA 480 | | TCAGACTGCA CCCTGAAGAT CCTTGACTTT GGCCTGGCCC GGACAGCGTG CACTAACTTC 540 | | ATGATGACCC CTTACGTGGT GACACGGTAC TACCGGGCGC CCGAAGTCAT CCTGGGTATG 600 | | GGCTACAAAG AGAACGTGGA CATCTGGTCT GTCGGGTGCA TCATGGCAGA AATGGTCCTC 660 | | CATAAAGTCC TGTTCCCGGG AAGAGACTAT ATTGATCAGT GGAATAAAGT TATTGAGCAG 720 | | CTGGGAACAC CATCAGCAGA GTTCATGAAG AAACTTCAGC CAACTGTGAG GAATTATGTC 780 | | GAAAACAGAC CAAAGTATCC TGGAATCAAA TTTGAAGAAC TCTTTCCAGA TTGGATATTC 840 | | CCATCAGAAT CTGAGCGAGA CAAAATAAAA ACAAGTCAAG CCAGAGATCT GTTATCAAAA 900 | | ATGTTAGTGA TTGATCCTGA CAAGCGGATC TCTGTAGACG AAGCTCTGCG TCACCCATAC 960 | | ATCACTGTTT GGTATGACCC CGCCGAAGCA GAAGCCCCAC CACCTCAAAT TTATGATGCC 1020 | | CAGTTGGAAG AAAGAGAACA TGCAATTGAA GAATGGAAAG AGCTAATTTA CAAAGAAGTC 1080 | | ATGGATTGGG AAGAAAGAAG CAAGAATGGT GTTGTAAAAG ATCAGCCTTC AGCACAGATG 1140 | | CAGCAGTAA 1149 |
|
| Domain Profile | S: 1 yeslkplgeGaygvvvsavdkrteervaikklsrpfqketsakrtlRElkllkelkheNi 60 | y++lkp+g+Ga+g+v++a+d+ + +va+kklsrpfq++t+akr++REl llk+++h+Ni | Q: 26 YQQLKPIGSGAQGIVCAAFDTVLGINVAVKKLSRPFQNQTHAKRAYRELVLLKCVNHKNI 85 | 899********************************************************* |
| S: 61 iklldvftpeeeleelkdvYlvtelmetdLkkviksqklsdehiklllyqilrglkylHs 120 | i+ll+vftp+++lee++dvYlv+elm+++L++vi+++ l++e++++llyq+l+g+k+lHs | Q: 86 ISLLNVFTPQKTLEEFQDVYLVMELMDANLCQVIHME-LDHERMSYLLYQMLCGIKHLHS 144 | ************************************9.********************** |
| S: 121 anviHrDlKPsNllvnedcelkildFGlarsadkekekklteyvatrwYraPeillslke 180 | a++iHrDlKPsN++v++dc+lkildFGlar+a ++ + +t+yv+tr+YraPe++l + | Q: 145 AGIIHRDLKPSNIVVKSDCTLKILDFGLARTACTNFM--MTPYVVTRYYRAPEVILG-MG 201 | ********************************99976..*****************9.99 |
| S: 181 ytkavDiWsvGCIlaElltgkplfpgkdeidqlekilevlgtpseeflkkieseearnyi 240 | y+++vDiWsvGCI+aE++ +k+lfpg+d+idq++k++e+lgtps+ef+kk++ ++rny+ | Q: 202 YKENVDIWSVGCIMAEMVLHKVLFPGRDYIDQWNKVIEQLGTPSAEFMKKLQP-TVRNYV 260 | *****************************************************.5***** |
| S: 241 kslpkkkkkdfeelfpk............aseealdLleklLvldpdkRisveeaLehpY 288 | +++pk++ +feelfp+ ++++a+dLl+k+Lv+dpdkRisv+eaL+hpY | Q: 261 ENRPKYPGIKFEELFPDwifpseserdkiKTSQARDLLSKMLVIDPDKRISVDEALRHPY 320 | *****************************9****************************** |
| S: 289 l 289 | + | Q: 321 I 321 | 7 |
|
Domain Sequence
(FASTA) | | YQQLKPIGSG AQGIVCAAFD TVLGINVAVK KLSRPFQNQT HAKRAYRELV LLKCVNHKNI 60 | | ISLLNVFTPQ KTLEEFQDVY LVMELMDANL CQVIHMELDH ERMSYLLYQM LCGIKHLHSA 120 | | GIIHRDLKPS NIVVKSDCTL KILDFGLART ACTNFMMTPY VVTRYYRAPE VILGMGYKEN 180 | | VDIWSVGCIM AEMVLHKVLF PGRDYIDQWN KVIEQLGTPS AEFMKKLQPT VRNYVENRPK 240 | | YPGIKFEELF PDWIFPSESE RDKIKTSQAR DLLSKMLVID PDKRISVDEA LRHPYI 296 |
|
| Keyword | 3D-structure; Alternative splicing; ATP-binding; Complete proteome; Cytoplasm; Kinase; Nucleotide-binding; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Serine/threonine-protein kinase; Transferase. |
| Sequence Source | Ensembl |
| Orthology | |
| Gene Ontology | |
| KEGG | |
| InterPros | |
| Pfam | |
| SMARTs | |
| Prosites | |
| Prints | |
| Created Date | 20-Feb-2013 |