| Tag | Content |
|---|
| EKPD ID | EKS-HOS-00181 |
| Classification | | Group/Family | Score | E-Value | Start | End | Domain Length |
|---|
| AGC/PKC | 438.8 | 3.7E-131 | 255 | 509 | 255 |
|
| Status | Reviewed |
| Ensembl Protein | ENSP00000441752 |
| UniProt Accession | Q04759; Q14DH6; Q3MJF1; Q64FY5; Q9H508; Q9H549; |
| Protein Name | Protein kinase C theta type |
| Protein Synonyms/Alias | nPKC-theta; |
| Gene Name | PRKCQ |
| Gene Synonyms/Alias | PRKCQ; PRKCT; |
| Ensembl Information | |
| Organism | Homo sapiens |
| Functional Description | Calcium-independent, phospholipid- and diacylglycerol(DAG)-dependent serine/threonine-protein kinase that mediates non- redundant functions in T-cell receptor (TCR) signaling, including T-cells activation, proliferation, differentiation and survival, by mediating activation of multiple transcription factors such as NF-kappa-B, JUN, NFATC1 and NFATC2. In TCR-CD3/CD28-co-stimulated T-cells, is required for the activation of NF-kappa-B and JUN, which in turn are essential for IL2 production, and participates to the calcium-dependent NFATC1 and NFATC2 transactivation. Mediates the activation of the canonical NF-kappa-B pathway (NFKB1) by direct phosphorylation of CARD11 on several serine residues, inducing CARD11 association with lipid rafts and recruitment of the BCL10-MALT1 complex, which then activates IKK complex, resulting in nuclear translocation and activation of NFKB1. May also play an indirect role in activation of the non- canonical NF-kappa-B (NFKB2) pathway. In the signaling pathway leading to JUN activation, acts by phosphorylating the mediator STK39/SPAK and may not act through MAP kinases signaling. Plays a critical role in TCR/CD28-induced NFATC1 and NFATC2 transactivation by participating in the regulation of reduced inositol 1,4,5-trisphosphate generation and intracellular calcium mobilization. After costimulation of T-cells through CD28 can phosphorylate CBLB and is required for the ubiquitination and subsequent degradation of CBLB, which is a prerequisite for the activation of TCR. During T-cells differentiation, plays an important role in the development of T-helper 2 (Th2) cells following immune and inflammatory responses, and, in the development of inflammatory autoimmune diseases, is necessary for the activation of IL17-producing Th17 cells. May play a minor role in Th1 response. Upon TCR stimulation, mediates T-cell protective survival signal by phosphorylating BAD, thus protecting T-cells from BAD-induced apoptosis, and by up-regulating BCL-X(L)/BCL2L1 levels through NF-kappa-B and JUN pathways. In platelets, regulates signal transduction downstream of the ITGA2B, CD36/GP4, F2R/PAR1 and F2RL3/PAR4 receptors, playing a positive role in 'outside-in' signaling and granule secretion signal transduction. May relay signals from the activated ITGA2B receptor by regulating the uncoupling of WASP and WIPF1, thereby permitting the regulation of actin filament nucleation and branching activity of the Arp2/3 complex. May mediate inhibitory effects of free fatty acids on insulin signaling by phosphorylating IRS1, which in turn blocks IRS1 tyrosine phosphorylation and downstream activation of the PI3K/AKT pathway. Phosphorylates MSN (moesin) in the presence of phosphatidylglycerol or phosphatidylinositol. Phosphorylates PDPK1 at 'Ser-504' and 'Ser-532' and negatively regulates its ability to phosphorylate PKB/AKT1. |
| Protein Length | 581 |
Protein Sequence
(FASTA) | | MDTKDMNEFE TEGFFALHQR RGAIKQAKVH HVKCHEFTAT FFPQPTFCSV CHEFVWGLNK 60 | | QGYQCRQCNA AIHKKCIDKV IAKCTGSAIN SRETMFHKER FKIDMPHRFK VYNYKSPTFC 120 | | EHCGTLLWGL ARQGLKCDAC GMNVHHRCQT KVANLCGINQ KLMAEALAMI ESTQQARCLR 180 | | DTEQIFREGP VEIGLPCSIK NEARPPCLPT PGKREPQGIS WESPLDEVDK MCHLPEPELN 240 | | KERPSLQIKL KIEDFILHKM LGKGSFGKVF LAEFKKTNQF FAIKALKKDV VLMDDDVECT 300 | | MVEKRVLSLA WEHPFLTHMF CTFQTKENLF FVMEYLNGGD LMYHIQSCHK FDLSRATFYA 360 | | AEIILGLQFL HSKGIVYRDL KLDNILLDKD GHIKIADFGM CKENMLGDAK TNTFCGTPDY 420 | | IAPEILLGQK YNHSVDWWSF GVLLYEMLIG QSPFHGQDEE ELFHSIRMDN PFYPRWLEKE 480 | | AKDLLVKLFV REPEKRLGVR GDIRQHPLFR EINWEELERK EIDPPFRPKV KSPFDCSNFD 540 | | KEFLNEKPRL SFADRALINS MDQNMFRNFS FMNPGMERLI S 581 |
|
Nucleotide Sequence
(FASTA) | | ATGGACACAA AGGACATGAA TGAATTTGAG ACGGAAGGCT TCTTTGCTTT GCATCAGCGC 60 | | CGGGGTGCCA TCAAGCAGGC AAAGGTCCAC CACGTCAAGT GCCACGAGTT CACTGCCACC 120 | | TTCTTCCCAC AGCCCACATT TTGCTCTGTC TGCCACGAGT TTGTCTGGGG CCTGAACAAA 180 | | CAGGGCTACC AGTGCCGACA ATGCAATGCA GCAATTCACA AGAAGTGTAT TGATAAAGTT 240 | | ATAGCAAAGT GCACAGGATC AGCTATCAAT AGCCGAGAAA CCATGTTCCA CAAGGAGAGA 300 | | TTCAAAATTG ACATGCCACA CAGATTTAAA GTCTACAATT ACAAGAGCCC GACCTTCTGT 360 | | GAACACTGTG GGACCCTGCT GTGGGGACTG GCACGGCAAG GACTCAAGTG TGATGCATGT 420 | | GGCATGAATG TGCATCATAG ATGCCAGACA AAGGTGGCCA ACCTTTGTGG CATAAACCAG 480 | | AAGCTAATGG CTGAAGCGCT GGCCATGATT GAGAGCACTC AACAGGCTCG CTGCTTAAGA 540 | | GATACTGAAC AGATCTTCAG AGAAGGTCCG GTTGAAATTG GTCTCCCATG CTCCATCAAA 600 | | AATGAAGCAA GGCCGCCATG TTTACCGACA CCGGGAAAAA GAGAGCCTCA GGGCATTTCC 660 | | TGGGAGTCTC CGTTGGATGA GGTGGATAAA ATGTGCCATC TTCCAGAACC TGAACTGAAC 720 | | AAAGAAAGAC CATCTCTGCA GATTAAACTA AAAATTGAGG ATTTTATCTT GCACAAAATG 780 | | TTGGGGAAAG GAAGTTTTGG CAAGGTCTTC CTGGCAGAAT TCAAGAAAAC CAATCAATTT 840 | | TTCGCAATAA AGGCCTTAAA GAAAGATGTG GTCTTGATGG ACGATGATGT TGAGTGCACG 900 | | ATGGTAGAGA AGAGAGTTCT TTCCTTGGCC TGGGAGCATC CGTTTCTGAC GCACATGTTT 960 | | TGTACATTCC AGACCAAGGA AAACCTCTTT TTTGTGATGG AGTACCTCAA CGGAGGGGAC 1020 | | TTAATGTACC ACATCCAAAG CTGCCACAAG TTCGACCTTT CCAGAGCGAC GTTTTATGCT 1080 | | GCTGAAATCA TTCTTGGTCT GCAGTTCCTT CATTCCAAAG GAATAGTCTA CAGGGACCTG 1140 | | AAGCTAGATA ACATCCTGTT AGACAAAGAT GGACATATCA AGATCGCGGA TTTTGGAATG 1200 | | TGCAAGGAGA ACATGTTAGG AGATGCCAAG ACGAATACCT TCTGTGGGAC ACCTGACTAC 1260 | | ATCGCCCCAG AGATCTTGCT GGGTCAGAAA TACAACCACT CTGTGGACTG GTGGTCCTTC 1320 | | GGGGTTCTCC TTTATGAAAT GCTGATTGGT CAGTCGCCTT TCCACGGGCA GGATGAGGAG 1380 | | GAGCTCTTCC ACTCCATCCG CATGGACAAT CCCTTTTACC CACGGTGGCT GGAGAAGGAA 1440 | | GCAAAGGACC TTCTGGTGAA GCTCTTCGTG CGAGAACCTG AGAAGAGGCT GGGCGTGAGG 1500 | | GGAGACATCC GCCAGCACCC TTTGTTTCGG GAGATCAACT GGGAGGAACT TGAACGGAAG 1560 | | GAGATTGACC CACCGTTCCG GCCGAAAGTG AAATCACCAT TTGACTGCAG CAATTTCGAC 1620 | | AAAGAATTCT TAAACGAGAA GCCCCGGCTG TCATTTGCCG ACAGAGCACT GATCAACAGC 1680 | | ATGGACCAGA ATATGTTCAG GAACTTTTCC TTCATGAACC CCGGGATGGA GCGGCTGATA 1740 | | TCCTGA 1746 |
|
| Domain Profile | S: 1 fellkvlGkGsfgkvllaelkktdelyaikvlkkdvvlqdddveltlvekrvlalaskkp 60 | f l+k+lGkGsfgkv+lae kkt++++aik lkkdvvl+dddve+t+vekrvl+la+++p | Q: 255 FILHKMLGKGSFGKVFLAEFKKTNQFFAIKALKKDVVLMDDDVECTMVEKRVLSLAWEHP 314 | 7899******************************************************** |
| S: 61 flvqlfscfqtedrlffvleyvnGGdlmfhiqkarklkeerarfyaaeiilalkflhekg 120 | fl+++f++fqt+++lffv+ey+nGGdlm+hiq+++k++ +ra+fyaaeiil+l+flh+kg | Q: 315 FLTHMFCTFQTKENLFFVMEYLNGGDLMYHIQSCHKFDLSRATFYAAEIILGLQFLHSKG 374 | ************************************************************ |
| S: 121 iiyrdlkldnvlldaeGhikladfGlckeeilegkttstfcGtPdyiaPeilkeeeygks 180 | i+yrdlkldn+lld++Ghik+adfG+cke++l +++t+tfcGtPdyiaPeil +++y++s | Q: 375 IVYRDLKLDNILLDKDGHIKIADFGMCKENMLGDAKTNTFCGTPDYIAPEILLGQKYNHS 434 | ************************************************************ |
| S: 181 vdwwalGvllyemlagqsPfegededelfesilekevlyPkslskeaveilkglltkdpe 240 | vdww++Gvllyeml+gqsPf+g+de+elf+si++++++yP++l+kea+++l +l+ ++pe | Q: 435 VDWWSFGVLLYEMLIGQSPFHGQDEEELFHSIRMDNPFYPRWLEKEAKDLLVKLFVREPE 494 | ************************************************************ |
| S: 241 krlGvkeegeedikehaff 259 | krlGv +g di++h++f | Q: 495 KRLGV--RG--DIRQHPLF 509 | *****..55..89999987 |
|
Domain Sequence
(FASTA) | | FILHKMLGKG SFGKVFLAEF KKTNQFFAIK ALKKDVVLMD DDVECTMVEK RVLSLAWEHP 60 | | FLTHMFCTFQ TKENLFFVME YLNGGDLMYH IQSCHKFDLS RATFYAAEII LGLQFLHSKG 120 | | IVYRDLKLDN ILLDKDGHIK IADFGMCKEN MLGDAKTNTF CGTPDYIAPE ILLGQKYNHS 180 | | VDWWSFGVLL YEMLIGQSPF HGQDEEELFH SIRMDNPFYP RWLEKEAKDL LVKLFVREPE 240 | | KRLGVRGDIR QHPLF 255 |
|
| Keyword | 3D-structure; Acetylation; Alternative splicing; ATP-binding; Cell membrane; Complete proteome; Cytoplasm; Immunity; Inflammatory response; Kinase; Magnesium; Membrane; Metal-binding; Nucleotide-binding; Phosphoprotein; Polymorphism; Reference proteome; Repeat; Serine/threonine-protein kinase; Transferase; Zinc; Zinc-finger. |
| Sequence Source | Ensembl |
| Orthology | |
| Gene Ontology | |
| KEGG | |
| InterPros | |
| Pfam | |
| SMARTs | |
| Prosites | |
| Prints | |
| Created Date | 20-Feb-2013 |