EKS-HOS-00449

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EKS-HOS-00449

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
AGC/DMPK	470.4	8.3E-141	76	338	263

Status

Reviewed

Ensembl Protein

ENSP00000382697

UniProt Accession

Q13464; B0YJ91; Q2KHM4; Q59GZ4;

Protein Name

Rho-associated protein kinase 1

Protein Synonyms/Alias

Renal carcinoma antigen NY-REN-35; Rho-associated, coiled-coil-containing protein kinase 1; Rho-associated, coiled-coil-containing protein kinase I; ROCK-I; p160 ROCK-1; p160ROCK;

Gene Name

ROCK1

Gene Synonyms/Alias

ROCK1;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
ENSG00000067900	ENSP00000382697	ENST00000399799
ENSG00000067900	ENSP00000462004	ENST00000578051

Organism

Homo sapiens

Functional Description

Protein kinase which is a key regulator of actincytoskeleton and cell polarity. Involved in regulation of smooth muscle contraction, actin cytoskeleton organization, stress fiber and focal adhesion formation, neurite retraction, cell adhesion and motility via phosphorylation of DAPK3, GFAP, LIMK1, LIMK2, MYL9/MLC2, PFN1 and PPP1R12A. Phosphorylates FHOD1 and acts synergistically with it to promote SRC-dependent non-apoptotic plasma membrane blebbing. Phosphorylates JIP3 and regulates the recruitment of JNK to JIP3 upon UVB-induced stress. Acts as a suppressor of inflammatory cell migration by regulating PTEN phosphorylation and stability. Acts as a negative regulator of VEGF-induced angiogenic endothelial cell activation. Required for centrosome positioning and centrosome-dependent exit from mitosis. Plays a role in terminal erythroid differentiation. May regulate closure of the eyelids and ventral body wall by inducing the assembly of actomyosin bundles. Promotes keratinocyte terminal differentiation.

Protein Length

1354

Protein Sequence
(FASTA)

MSTGDSFETR FEKMDNLLRD PKSEVNSDCL LDGLDALVYD LDFPALRKNK NIDNFLSRYK 60

DTINKIRDLR MKAEDYEVVK VIGRGAFGEV QLVRHKSTRK VYAMKLLSKF EMIKRSDSAF 120

FWEERDIMAF ANSPWVVQLF YAFQDDRYLY MVMEYMPGGD LVNLMSNYDV PEKWARFYTA 180

EVVLALDAIH SMGFIHRDVK PDNMLLDKSG HLKLADFGTC MKMNKEGMVR CDTAVGTPDY 240

ISPEVLKSQG GDGYYGRECD WWSVGVFLYE MLVGDTPFYA DSLVGTYSKI MNHKNSLTFP 300

DDNDISKEAK NLICAFLTDR EVRLGRNGVE EIKRHLFFKN DQWAWETLRD TVAPVVPDLS 360

SDIDTSNFDD LEEDKGEEET FPIPKAFVGN QLPFVGFTYY SNRRYLSSAN PNDNRTSSNA 420

DKSLQESLQK TIYKLEEQLH NEMQLKDEME QKCRTSNIKL DKIMKELDEE GNQRRNLEST 480

VSQIEKEKML LQHRINEYQR KAEQENEKRR NVENEVSTLK DQLEDLKKVS QNSQLANEKL 540

SQLQKQLEEA NDLLRTESDT AVRLRKSHTE MSKSISQLES LNRELQERNR ILENSKSQTD 600

KDYYQLQAIL EAERRDRGHD SEMIGDLQAR ITSLQEEVKH LKHNLEKVEG ERKEAQDMLN 660

HSEKEKNNLE IDLNYKLKSL QQRLEQEVNE HKVTKARLTD KHQSIEEAKS VAMCEMEKKL 720

KEEREAREKA ENRVVQIEKQ CSMLDVDLKQ SQQKLEHLTG NKERMEDEVK NLTLQLEQES 780

NKRLLLQNEL KTQAFEADNL KGLEKQMKQE INTLLEAKRL LEFELAQLTK QYRGNEGQMR 840

ELQDQLEAEQ YFSTLYKTQV KELKEEIEEK NRENLKKIQE LQNEKETLAT QLDLAETKAE 900

SEQLARGLLE EQYFELTQES KKAASRNRQE ITDKDHTVSR LEEANSMLTK DIEILRRENE 960

ELTEKMKKAE EEYKLEKEEE ISNLKAAFEK NINTERTLKT QAVNKLAEIM NRKDFKIDRK 1020

KANTQDLRKK EKENRKLQLE LNQEREKFNQ MVVKHQKELN DMQAQLVEEC AHRNELQMQL 1080

ASKESDIEQL RAKLLDLSDS TSVASFPSAD ETDGNLPESR IEGWLSVPNR GNIKRYGWKK 1140

QYVVVSSKKI LFYNDEQDKE QSNPSMVLDI DKLFHVRPVT QGDVYRAETE EIPKIFQILY 1200

ANEGECRKDV EMEPVQQAEK TNFQNHKGHE FIPTLYHFPA NCDACAKPLW HVFKPPPALE 1260

CRRCHVKCHR DHLDKKEDLI CPCKVSYDVT SARDMLLLAC SQDEQKKWVT HLVKKIPKNP 1320

PSGFVRASPR TLSTRSTANQ SFRKVVKNTS GKTS 1354

Nucleotide Sequence
(FASTA)

ATGTCGACTG GGGACAGTTT TGAGACTCGA TTTGAAAAAA TGGACAACCT GCTGCGGGAT 60

CCCAAATCGG AAGTGAATTC GGATTGTTTG CTGGATGGAT TGGATGCTTT GGTATATGAT 120

TTGGATTTTC CTGCCTTAAG AAAAAACAAA AATATTGACA ACTTTTTAAG CAGATATAAA 180

GACACAATAA ATAAAATCAG AGATTTACGA ATGAAAGCTG AAGATTATGA AGTAGTGAAG 240

GTGATTGGTA GAGGTGCATT TGGAGAAGTT CAATTGGTAA GGCATAAATC CACCAGGAAG 300

GTATATGCTA TGAAGCTTCT CAGCAAATTT GAAATGATAA AGAGATCTGA TTCTGCTTTT 360

TTCTGGGAAG AAAGGGACAT CATGGCTTTT GCCAACAGTC CTTGGGTTGT TCAGCTTTTT 420

TATGCATTCC AAGATGATCG TTATCTCTAC ATGGTGATGG AATACATGCC TGGTGGAGAT 480

CTTGTAAACT TAATGAGCAA CTATGATGTG CCTGAAAAAT GGGCACGATT CTATACTGCA 540

GAAGTAGTTC TTGCATTGGA TGCAATCCAT TCCATGGGTT TTATTCACAG AGATGTGAAG 600

CCTGATAACA TGCTGCTGGA TAAATCTGGA CATTTGAAGT TAGCAGATTT TGGTACTTGT 660

ATGAAGATGA ATAAGGAAGG CATGGTACGA TGTGATACAG CGGTTGGAAC ACCTGATTAT 720

ATTTCCCCTG AAGTATTAAA ATCCCAAGGT GGTGATGGTT ATTATGGAAG AGAATGTGAC 780

TGGTGGTCGG TTGGGGTATT TTTATACGAA ATGCTTGTAG GTGATACACC TTTTTATGCA 840

GATTCTTTGG TTGGAACTTA CAGTAAAATT ATGAACCATA AAAATTCACT TACCTTTCCT 900

GATGATAATG ACATATCAAA AGAAGCAAAA AACCTTATTT GTGCCTTCCT TACTGACAGG 960

GAAGTGAGGT TAGGGCGAAA TGGTGTAGAA GAAATCAAAC GACATCTCTT CTTCAAAAAT 1020

GACCAGTGGG CTTGGGAAAC GCTCCGAGAC ACTGTAGCAC CAGTTGTACC CGATTTAAGT 1080

AGTGACATTG ATACTAGTAA TTTTGATGAC TTGGAAGAAG ATAAAGGAGA GGAAGAAACA 1140

TTCCCTATTC CTAAAGCTTT CGTTGGCAAT CAACTACCTT TTGTAGGATT TACATATTAT 1200

AGCAATCGTA GATACTTATC TTCAGCAAAT CCTAATGATA ACAGAACTAG CTCCAATGCA 1260

GATAAAAGCT TGCAGGAAAG TTTGCAAAAA ACAATCTATA AGCTGGAAGA ACAGCTGCAT 1320

AATGAAATGC AGTTAAAAGA TGAAATGGAG CAGAAGTGCA GAACCTCAAA CATAAAACTA 1380

GACAAGATAA TGAAAGAATT GGATGAAGAG GGAAATCAAA GAAGAAATCT AGAATCTACA 1440

GTGTCTCAGA TTGAGAAGGA GAAAATGTTG CTACAGCATA GAATTAATGA GTACCAAAGA 1500

AAAGCTGAAC AGGAAAATGA GAAGAGAAGA AATGTAGAAA ATGAAGTTTC TACATTAAAG 1560

GATCAGTTGG AAGACTTAAA GAAAGTCAGT CAGAATTCAC AGCTTGCTAA TGAGAAGCTG 1620

TCCCAGTTAC AAAAGCAGCT AGAAGAAGCC AATGACTTAC TTAGGACAGA ATCGGACACA 1680

GCTGTAAGAT TGAGGAAGAG TCACACAGAG ATGAGCAAGT CAATTAGTCA GTTAGAGTCC 1740

CTGAACAGAG AGTTGCAAGA GAGAAATCGA ATTTTAGAGA ATTCTAAGTC ACAAACAGAC 1800

AAAGATTATT ACCAGCTGCA AGCTATATTA GAAGCTGAAC GAAGAGACAG AGGTCATGAT 1860

TCTGAGATGA TTGGAGACCT TCAAGCTCGA ATTACATCTT TACAAGAGGA GGTGAAGCAT 1920

CTCAAACATA ATCTCGAAAA AGTGGAAGGA GAAAGAAAAG AGGCTCAAGA CATGCTTAAT 1980

CACTCAGAAA AGGAAAAGAA TAATTTAGAG ATAGATTTAA ACTACAAACT TAAATCATTA 2040

CAACAACGGT TAGAACAAGA GGTAAATGAA CACAAAGTAA CCAAAGCTCG TTTAACTGAC 2100

AAACATCAAT CTATTGAAGA GGCAAAGTCT GTGGCAATGT GTGAGATGGA AAAAAAGCTG 2160

AAAGAAGAAA GAGAAGCTCG AGAGAAGGCT GAAAATCGGG TTGTTCAGAT TGAGAAACAG 2220

TGTTCCATGC TAGACGTTGA TCTGAAGCAA TCTCAGCAGA AACTAGAACA TTTGACTGGA 2280

AATAAAGAAA GGATGGAGGA TGAAGTTAAG AATCTAACCC TGCAACTGGA GCAGGAATCA 2340

AATAAGCGGC TGTTGTTACA AAATGAATTG AAGACTCAAG CATTTGAGGC AGACAATTTA 2400

AAAGGTTTAG AAAAGCAGAT GAAACAGGAA ATAAATACTT TATTGGAAGC AAAGAGATTA 2460

TTAGAATTTG AGTTAGCTCA GCTTACGAAA CAGTATAGAG GAAATGAAGG ACAGATGCGG 2520

GAGCTACAAG ATCAGCTTGA AGCTGAGCAA TATTTCTCGA CACTTTATAA AACCCAGGTA 2580

AAGGAACTTA AAGAAGAAAT TGAAGAAAAA AACAGAGAAA ATTTAAAGAA AATACAGGAA 2640

CTACAAAATG AAAAAGAAAC TCTTGCTACT CAGTTGGATC TAGCAGAAAC AAAAGCTGAG 2700

TCTGAGCAGT TGGCGCGAGG CCTTCTGGAA GAACAGTATT TTGAATTGAC GCAAGAAAGC 2760

AAGAAAGCTG CTTCAAGAAA TAGACAAGAG ATTACAGATA AAGATCACAC TGTTAGTCGG 2820

CTTGAAGAAG CAAACAGCAT GCTAACCAAA GATATTGAAA TATTAAGAAG AGAGAATGAA 2880

GAGCTAACAG AGAAAATGAA GAAGGCAGAG GAAGAATATA AACTGGAGAA GGAGGAGGAG 2940

ATCAGTAATC TTAAGGCTGC CTTTGAAAAG AATATCAACA CTGAACGAAC CCTTAAAACA 3000

CAGGCTGTTA ACAAATTGGC AGAAATAATG AATCGAAAAG ATTTTAAAAT TGATAGAAAG 3060

AAAGCTAATA CACAAGATTT GAGAAAGAAA GAAAAGGAAA ATCGAAAGCT GCAACTGGAA 3120

CTCAACCAAG AAAGAGAGAA ATTCAACCAG ATGGTAGTGA AACATCAGAA GGAACTGAAT 3180

GACATGCAAG CGCAATTGGT AGAAGAATGT GCACATAGGA ATGAGCTTCA GATGCAGTTG 3240

GCCAGCAAAG AGAGTGATAT TGAGCAATTG CGTGCTAAAC TTTTGGACCT CTCGGATTCT 3300

ACAAGTGTTG CTAGTTTTCC TAGTGCTGAT GAAACTGATG GTAACCTCCC AGAGTCAAGA 3360

ATTGAAGGTT GGCTTTCAGT ACCAAATAGA GGAAATATCA AACGATATGG CTGGAAGAAA 3420

CAGTATGTTG TGGTAAGCAG CAAAAAAATT TTGTTCTATA ATGACGAACA AGATAAGGAG 3480

CAATCCAATC CATCTATGGT ATTGGACATA GATAAACTGT TTCACGTTAG ACCTGTAACC 3540

CAAGGAGATG TGTATAGAGC TGAAACTGAA GAAATTCCTA AAATATTCCA GATACTATAT 3600

GCAAATGAAG GTGAATGTAG AAAAGATGTA GAGATGGAAC CAGTACAACA AGCTGAAAAA 3660

ACTAATTTCC AAAATCACAA AGGCCATGAG TTTATTCCTA CACTCTACCA CTTTCCTGCC 3720

AATTGTGATG CCTGTGCCAA ACCTCTCTGG CATGTTTTTA AGCCACCCCC TGCCCTAGAG 3780

TGTCGAAGAT GCCATGTTAA GTGCCACAGA GATCACTTAG ATAAGAAAGA GGACTTAATT 3840

TGTCCATGTA AAGTAAGTTA TGATGTAACA TCAGCAAGAG ATATGCTGCT GTTAGCATGT 3900

TCTCAGGATG AACAAAAAAA ATGGGTAACT CATTTAGTAA AGAAAATCCC TAAGAATCCA 3960

CCATCTGGTT TTGTTCGTGC TTCCCCTCGA ACGCTTTCTA CAAGATCCAC TGCAAATCAG 4020

TCTTTCCGGA AAGTGGTCAA AAATACATCT GGAAAAACTA GTTAA 4065

Domain Profile

S: 1     feilkviGrGafgevavvrekatekvyamkilkklellkraesaffreerdvlvkadsew  60

         +e++kviGrGafgev++vr+k+t+kvyamk+l+k+e++kr++saff+eerd++++a+s+w

Q: 76    YEVVKVIGRGAFGEVQLVRHKSTRKVYAMKLLSKFEMIKRSDSAFFWEERDIMAFANSPW  135

         89**********************************************************

S: 61    ivqlqyafqdekylylvmeylvGGdlltllskyedklpeelarfylaelvlaldsvhelg  120

         +vql+yafqd++yly+vmey++GGdl++l+s+y+  +pe++arfy+ae+vlald++h++g

Q: 136   VVQLFYAFQDDRYLYMVMEYMPGGDLVNLMSNYD--VPEKWARFYTAEVVLALDAIHSMG  193

         **********************************..9***********************

S: 121   yvhrdikpdnilldksGhikladfGsclklnedgtvkssvavGtpdyispevlkaleegk  180

         ++hrd+kpdn+lldksGh+kladfG+c+k+n++g+v++++avGtpdyispevlk+++ g+

Q: 194   FIHRDVKPDNMLLDKSGHLKLADFGTCMKMNKEGMVRCDTAVGTPDYISPEVLKSQG-GD  252

         ********************************************************9.**

S: 181   geygkecdwwslGvilyellvgetpfyaeslvetyskilnhkeslkfpedekvseeakdl  240

         g+yg+ecdwws+Gv+lye+lvg+tpfya+slv tyski+nhk+sl+fp+d+++s+eak+l

Q: 253   GYYGRECDWWSVGVFLYEMLVGDTPFYADSLVGTYSKIMNHKNSLTFPDDNDISKEAKNL  312

         ************************************************************

S: 241   ikklltsreerlgregiedlkkhpff  266

         i+++lt+re rlgr+g+e++k+h ff

Q: 313   ICAFLTDREVRLGRNGVEEIKRHLFF  338

         *************************9

Domain Sequence
(FASTA)

YEVVKVIGRG AFGEVQLVRH KSTRKVYAMK LLSKFEMIKR SDSAFFWEER DIMAFANSPW 60

VVQLFYAFQD DRYLYMVMEY MPGGDLVNLM SNYDVPEKWA RFYTAEVVLA LDAIHSMGFI 120

HRDVKPDNML LDKSGHLKLA DFGTCMKMNK EGMVRCDTAV GTPDYISPEV LKSQGGDGYY 180

GRECDWWSVG VFLYEMLVGD TPFYADSLVG TYSKIMNHKN SLTFPDDNDI SKEAKNLICA 240

FLTDREVRLG RNGVEEIKRH LFF 263

Keyword

3D-structure; Acetylation; Apoptosis; ATP-binding; Cell projection; Coiled coil; Complete proteome; Cytoplasm; Cytoskeleton; Direct protein sequencing; Golgi apparatus; Kinase; Magnesium; Membrane; Metal-binding; Nucleotide-binding; Phosphoprotein; Polymorphism; Reference proteome; Serine/threonine-protein kinase; Transferase; Zinc; Zinc-finger.

Sequence Source

Ensembl

Orthology

Ortholog group

Ailuropoda melanoleuca"; ?>Anolis carolinensis"; ?>Bos taurus"; ?>Caenorhabditis elegans"; ?>Callithrix jacchus"; ?>Canis familiaris"; ?>Cavia porcellus"; ?>Ciona intestinalis"; ?>Ciona savignyi"; ?>Danio rerio"; ?>Drosophila melanogaster"; ?>Equus caballus"; ?>Felis catus"; ?>Gadus morhua"; ?>Gallus gallus"; ?>Gasterosteus aculeatus"; ?>Gorilla gorilla"; ?>Latimeria chalumnae"; ?>Loxodonta africana"; ?>Macaca mulatta"; ?>Meleagris gallopavo"; ?>Monodelphis domestica"; ?>Mus musculus"; ?>Mustela putorius furo"; ?>Myotis lucifugus"; ?>Nomascus leucogenys"; ?>Oreochromis niloticus"; ?>Ornithorhynchus anatinus"; ?>Oryctolagus cuniculus"; ?>Oryzias latipes"; ?>Otolemur garnettii"; ?>Pan troglodytes"; ?>Pelodiscus sinensis"; ?>Petromyzon marinus"; ?>Pongo abelii"; ?>Rattus norvegicus"; ?>Sarcophilus harrisii"; ?>Sus scrofa"; ?>Taeniopygia guttata"; ?>Takifugu rubripes"; ?>Tetraodon nigroviridis"; ?>Vicugna pacos"; ?>Xenopus tropicalis"; ?>Xiphophorus maculatus"; ?>

Gene Ontology

GO:0032059	; C:bleb
GO:0005814	; C:centriole
GO:0005829	; C:cytosol
GO:0000139	; C:Golgi membrane
GO:0005524	; F:ATP binding
GO:0046872	; F:metal ion binding
GO:0005543	; F:phospholipid binding
GO:0004674	; F:protein serine/threonine kinase activity
GO:0030036	; P:actin cytoskeleton organization
GO:0003383	; P:apical constriction
GO:0007411	; P:axon guidance
GO:0032060	; P:bleb assembly
GO:0006921	; P:cellular component disassembly involved in execution phase of apoptosis
GO:0000910	; P:cytokinesis
GO:0050901	; P:leukocyte tethering or rolling
GO:0022614	; P:membrane to membrane docking
GO:0016525	; P:negative regulation of angiogenesis
GO:0043524	; P:negative regulation of neuron apoptotic process
GO:2000145	; P:regulation of cell motility
GO:2000114	; P:regulation of establishment of cell polarity
GO:0051893	; P:regulation of focal adhesion assembly
GO:0045616	; P:regulation of keratinocyte differentiation
GO:0051492	; P:regulation of stress fiber assembly
GO:0007266	; P:Rho protein signal transduction
GO:0006939	; P:smooth muscle contraction

KEGG

hsa:6093

;

InterPros

IPR000961	; AGC-kinase_C.
IPR011072	; HR1_rho-bd.
IPR011009	; Kinase-like_dom.
IPR011993	; PH_like_dom.
IPR017892	; Pkinase_C.
IPR001849	; Pleckstrin_homology.
IPR002219	; Prot_Kinase_C-like_PE/DAG-bd.
IPR000719	; Prot_kinase_cat_dom.
IPR017441	; Protein_kinase_ATP_BS.
IPR020684	; Rho-assoc_coiled-coil_kin.
IPR015008	; Rho-bd.
IPR002290	; Ser/Thr_dual-sp_kinase_dom.
IPR008271	; Ser/Thr_kinase_AS.

Pfam

PF02185	; HR1; 1.
PF00169	; PH; 1.
PF00069	; Pkinase; 1.
PF00433	; Pkinase_C; 1.
PF08912	; Rho_Binding; 1.

SMARTs

SM00109	; C1; 1.
SM00233	; PH; 1.
SM00133	; S_TK_X; 1.
SM00220	; S_TKc; 1.

Prosites

PS51285	; AGC_KINASE_CTER; 1.
PS50003	; PH_DOMAIN; 1.
PS00107	; PROTEIN_KINASE_ATP; 1.
PS50011	; PROTEIN_KINASE_DOM; 1.
PS00108	; PROTEIN_KINASE_ST; 1.
PS00479	; ZF_DAG_PE_1; FALSE_NEG.
PS50081	; ZF_DAG_PE_2; 1.

Prints

Created Date

20-Feb-2013