EKS-HOS-00337

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EKS-HOS-00337

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
STE/STE20	322.2	1.2E-95	36	294	259

Status

Reviewed

Ensembl Protein

ENSP00000176763

UniProt Accession

O94804; A6ND35; B2R8F5; B3KMY1; Q6NSK0; Q9UIW4;

Protein Name

Serine/threonine-protein kinase 10

Protein Synonyms/Alias

Lymphocyte-oriented kinase;

Gene Name

STK10

Gene Synonyms/Alias

STK10; LOK;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
ENSG00000072786	ENSP00000428806	ENST00000520476
ENSG00000072786	ENSP00000176763	ENST00000176763
ENSG00000072786	ENSP00000442209	ENST00000545839

Organism

Homo sapiens

Functional Description

Serine/threonine-protein kinase involved in regulationof lymphocyte migration. Phosphorylates MSN, and possibly PLK1. Involved in regulation of lymphocyte migration by mediating phosphorylation of ERM proteins such as MSN. Acts as a negative regulator of MAP3K1/MEKK1. May also act as a cell cycle regulator by acting as a polo kinase kinase: mediates phosphorylation of PLK1 in vitro; however such data require additional evidences in vivo.

Protein Length

968

Protein Sequence
(FASTA)

MAFANFRRIL RLSTFEKRKS REYEHVRRDL DPNEVWEIVG ELGDGAFGKV YKAKNKETGA 60

LAAAKVIETK SEEELEDYIV EIEILATCDH PYIVKLLGAY YHDGKLWIMI EFCPGGAVDA 120

IMLELDRGLT EPQIQVVCRQ MLEALNFLHS KRIIHRDLKA GNVLMTLEGD IRLADFGVSA 180

KNLKTLQKRD SFIGTPYWMA PEVVMCETMK DTPYDYKADI WSLGITLIEM AQIEPPHHEL 240

NPMRVLLKIA KSDPPTLLTP SKWSVEFRDF LKIALDKNPE TRPSAAQLLE HPFVSSITSN 300

KALRELVAEA KAEVMEEIED GRDEGEEEDA VDAASTLENH TQNSSEVSPP SLNADKPLEE 360

SPSTPLAPSQ SQDSVNEPCS QPSGDRSLQT TSPPVVAPGN ENGLAVPVPL RKSRPVSMDA 420

RIQVAQEKQV AEQGGDLSPA ANRSQKASQS RPNSSALETL GGEKLANGSL EPPAQAAPGP 480

SKRDSDCSSL CTSESMDYGT NLSTDLSLNK EMGSLSIKDP KLYKKTLKRT RKFVVDGVEV 540

SITTSKIISE DEKKDEEMRF LRRQELRELR LLQKEEHRNQ TQLSNKHELQ LEQMHKRFEQ 600

EINAKKKFFD TELENLERQQ KQQVEKMEQD HAVRRREEAR RIRLEQDRDY TRFQEQLKLM 660

KKEVKNEVEK LPRQQRKESM KQKMEEHTQK KQLLDRDFVA KQKEDLELAM KRLTTDNRRE 720

ICDKERECLM KKQELLRDRE AALWEMEEHQ LQERHQLVKQ QLKDQYFLQR HELLRKHEKE 780

REQMQRYNQR MIEQLKVRQQ QEKARLPKIQ RSEGKTRMAM YKKSLHINGG GSAAEQREKI 840

KQFSQQEEKR QKSERLQQQQ KHENQMRDML AQCESNMSEL QQLQNEKCHL LVEHETQKLK 900

ALDESHNQNL KEWRDKLRPR KKALEEDLNQ KKREQEMFFK LSEEAECPNP STPSKAAKFF 960

PYSSADAS 968

Nucleotide Sequence
(FASTA)

ATGGCTTTTG CCAATTTCCG CCGCATCCTG CGCCTGTCTA CCTTCGAGAA GAGAAAGTCC 60

CGCGAATATG AGCACGTCCG CCGCGACCTG GACCCCAACG AGGTGTGGGA GATCGTGGGC 120

GAGCTGGGCG ACGGCGCCTT CGGCAAGGTT TACAAGGCCA AGAATAAGGA GACGGGTGCT 180

TTGGCTGCGG CCAAAGTCAT TGAAACCAAG AGTGAGGAGG AGCTGGAGGA CTACATCGTG 240

GAGATTGAGA TCCTGGCCAC CTGCGACCAC CCCTACATTG TGAAGCTCCT GGGAGCCTAC 300

TATCACGACG GGAAGCTGTG GATCATGATT GAGTTCTGTC CAGGGGGAGC CGTGGACGCC 360

ATCATGCTGG AGCTGGACAG AGGCCTCACG GAGCCCCAGA TACAGGTGGT TTGCCGCCAG 420

ATGCTAGAAG CCCTCAACTT CCTGCACAGC AAGAGGATCA TCCACCGAGA TCTGAAAGCT 480

GGCAACGTGC TGATGACCCT CGAGGGAGAC ATCAGGCTGG CTGACTTTGG TGTGTCTGCC 540

AAGAATCTGA AGACTCTACA GAAACGAGAT TCCTTCATCG GCACGCCTTA CTGGATGGCC 600

CCCGAGGTGG TCATGTGTGA GACCATGAAA GACACGCCCT ACGACTACAA AGCCGACATC 660

TGGTCCCTGG GCATCACGCT GATTGAGATG GCCCAGATCG AGCCGCCACA CCACGAGCTC 720

AACCCCATGC GGGTCCTGCT AAAGATCGCC AAGTCGGACC CTCCCACGCT GCTCACGCCC 780

TCTAAGTGGT CTGTAGAGTT CCGTGACTTC CTGAAGATAG CCCTGGATAA GAACCCAGAA 840

ACCCGACCCA GTGCCGCGCA GCTGCTGGAG CATCCCTTCG TCAGCAGCAT CACCAGTAAC 900

AAGGCTCTGC GGGAGCTGGT GGCTGAGGCC AAGGCCGAGG TGATGGAAGA GATCGAAGAC 960

GGCCGGGATG AGGGGGAAGA GGAGGACGCC GTGGATGCCG CCTCCACCCT GGAGAACCAT 1020

ACTCAGAACT CCTCTGAGGT GAGTCCGCCA AGCCTCAATG CTGACAAGCC TCTCGAGGAG 1080

TCACCTTCCA CCCCGCTGGC ACCCAGCCAG TCTCAGGACA GTGTGAATGA GCCCTGCAGC 1140

CAGCCCTCTG GGGACAGATC CCTCCAAACC ACCAGTCCCC CAGTCGTGGC CCCTGGAAAT 1200

GAGAACGGCC TGGCAGTGCC TGTGCCCCTG CGGAAGTCCC GACCCGTGTC AATGGATGCC 1260

AGAATTCAGG TAGCCCAGGA GAAGCAAGTT GCTGAGCAGG GTGGGGACCT CAGCCCAGCA 1320

GCCAACAGAT CTCAAAAGGC CAGCCAGAGC CGGCCCAACA GCAGCGCCCT GGAGACCTTG 1380

GGTGGGGAGA AGCTGGCCAA TGGCAGCCTG GAGCCACCTG CCCAGGCAGC TCCAGGGCCT 1440

TCCAAGAGGG ACTCGGACTG CAGCAGCCTC TGCACCTCTG AGAGCATGGA CTATGGTACC 1500

AATCTCTCCA CTGACCTGTC GCTGAACAAA GAGATGGGCT CTCTGTCCAT CAAGGACCCG 1560

AAACTGTACA AAAAAACCCT CAAGCGGACA CGCAAATTTG TGGTGGATGG TGTGGAGGTG 1620

AGCATCACCA CCTCCAAGAT CATCAGCGAA GATGAGAAGA AGGATGAGGA GATGAGATTT 1680

CTCAGGCGCC AGGAACTCCG AGAGCTTCGG CTGCTCCAGA AAGAAGAGCA TCGGAACCAG 1740

ACCCAGCTGA GTAACAAGCA TGAGCTGCAG CTGGAGCAAA TGCATAAACG TTTTGAACAG 1800

GAAATCAACG CCAAGAAGAA GTTCTTTGAC ACGGAATTAG AGAACCTGGA GCGTCAGCAA 1860

AAGCAGCAAG TGGAGAAGAT GGAGCAAGAC CATGCCGTGC GCCGCCGGGA GGAGGCCAGG 1920

CGGATCCGCC TGGAGCAGGA TCGGGACTAC ACCAGGTTCC AAGAGCAGCT CAAACTGATG 1980

AAGAAAGAGG TGAAGAACGA GGTGGAGAAG CTCCCCCGAC AGCAGCGGAA GGAAAGCATG 2040

AAGCAGAAGA TGGAGGAGCA CACGCAGAAA AAGCAGCTTC TTGACCGGGA CTTTGTAGCC 2100

AAGCAGAAGG AGGACCTGGA GCTGGCCATG AAGAGGCTCA CCACCGACAA CAGGCGGGAG 2160

ATCTGTGACA AGGAGCGCGA GTGCCTCATG AAGAAGCAGG AGCTCCTTCG AGACCGGGAA 2220

GCAGCCCTGT GGGAGATGGA AGAGCACCAG CTGCAGGAGA GGCACCAGCT GGTGAAGCAG 2280

CAGCTCAAAG ACCAGTACTT CCTCCAGCGG CACGAGCTGC TGCGCAAGCA TGAGAAGGAG 2340

CGGGAGCAGA TGCAGCGCTA CAACCAGCGC ATGATAGAGC AGCTGAAGGT GCGGCAGCAA 2400

CAGGAAAAGG CGCGGCTGCC CAAGATCCAG AGGAGTGAGG GCAAGACGCG CATGGCCATG 2460

TACAAGAAGA GCCTCCACAT CAACGGCGGG GGCAGCGCAG CTGAGCAGCG TGAGAAGATC 2520

AAGCAGTTCT CCCAGCAGGA GGAGAAGAGG CAGAAGTCGG AGCGGCTGCA GCAACAGCAG 2580

AAACACGAGA ACCAGATGCG GGACATGCTG GCGCAGTGTG AGAGCAACAT GAGCGAGCTG 2640

CAGCAGCTGC AGAATGAAAA GTGCCACCTC CTGGTAGAGC ACGAAACCCA GAAACTGAAG 2700

GCCCTGGATG AGAGCCATAA CCAGAACCTG AAGGAATGGC GGGACAAGCT TCGGCCGCGC 2760

AAGAAGGCTC TGGAAGAGGA TCTGAACCAG AAGAAGCGGG AGCAGGAGAT GTTCTTCAAG 2820

CTGAGCGAGG AGGCGGAGTG CCCAAACCCC TCCACCCCAA GCAAGGCCGC CAAGTTCTTC 2880

CCCTACAGTT CTGCGGATGC TTCTTAA 2907

Domain Profile

S: 1     yelleeigkGalgkvylarekktgelvaikkinlekqekleelqkeisvlkklkhpnive  60

         +e++ e+g+Ga+gkvy+a++k+tg+l+a k+i+++++e+le+++ ei++l +++hp+iv+

Q: 36    WEIVGELGDGAFGKVYKAKNKETGALAAAKVIETKSEEELEDYIVEIEILATCDHPYIVK  95

         78999*******************************************************

S: 61    ylesflkeeelwlvmeylagGslsdlvkeelkselkEeqiayilrevlkaLeylHskkvi  120

         +l++++ + +lw+++e++ gG+++ ++  el+++l+E qi++++r++l+aL++lHsk++i

Q: 96    LLGAYYHDGKLWIMIEFCPGGAVDAIML-ELDRGLTEPQIQVVCRQMLEALNFLHSKRII  154

         ***************************9.9******************************

S: 121   HrdikakniLlteegeVkLsdfgvaaqlektrkkrktvvgtpywmAPEviaqe...ekgY  177

         Hrd+ka+n+L+t eg+++L+dfgv+a+  kt +kr++++gtpywmAPEv+ +e   ++ Y

Q: 155   HRDLKAGNVLMTLEGDIRLADFGVSAKNLKTLQKRDSFIGTPYWMAPEVVMCEtmkDTPY  214

         ****************************************************9998999*

S: 178   dekaDiwslGItaielaegepPfkdleplkallkilkneppklkskkkfskefkefvekc  237

         d+kaDiwslGIt+ie+a+ epP+++l+p+++llki+k++pp+l +++k+s ef++f++ +

Q: 215   DYKADIWSLGITLIEMAQIEPPHHELNPMRVLLKIAKSDPPTLLTPSKWSVEFRDFLKIA  274

         ************************************************************

S: 238   lqknpekRpsasellkhkFl  257

         l+knpe+Rpsa++ll+h+F+

Q: 275   LDKNPETRPSAAQLLEHPFV  294

         *******************7

Domain Sequence
(FASTA)

WEIVGELGDG AFGKVYKAKN KETGALAAAK VIETKSEEEL EDYIVEIEIL ATCDHPYIVK 60

LLGAYYHDGK LWIMIEFCPG GAVDAIMLEL DRGLTEPQIQ VVCRQMLEAL NFLHSKRIIH 120

RDLKAGNVLM TLEGDIRLAD FGVSAKNLKT LQKRDSFIGT PYWMAPEVVM CETMKDTPYD 180

YKADIWSLGI TLIEMAQIEP PHHELNPMRV LLKIAKSDPP TLLTPSKWSV EFRDFLKIAL 240

DKNPETRPSA AQLLEHPFV 259

Keyword

3D-structure; ATP-binding; Cell cycle; Cell membrane; Coiled coil; Complete proteome; Kinase; Membrane; Nucleotide-binding; Phosphoprotein; Polymorphism; Reference proteome; Serine/threonine-protein kinase; Transferase.

Sequence Source

Ensembl

Orthology

Ortholog group

Ailuropoda melanoleuca"; ?>Anolis carolinensis"; ?>Bos taurus"; ?>Callithrix jacchus"; ?>Canis familiaris"; ?>Cavia porcellus"; ?>Ciona savignyi"; ?>Equus caballus"; ?>Felis catus"; ?>Gallus gallus"; ?>Gasterosteus aculeatus"; ?>Gorilla gorilla"; ?>Ictidomys tridecemlineatus"; ?>Macaca mulatta"; ?>Meleagris gallopavo"; ?>Monodelphis domestica"; ?>Mus musculus"; ?>Mustela putorius furo"; ?>Myotis lucifugus"; ?>Nomascus leucogenys"; ?>Oreochromis niloticus"; ?>Oryctolagus cuniculus"; ?>Oryzias latipes"; ?>Otolemur garnettii"; ?>Pan troglodytes"; ?>Pongo abelii"; ?>Rattus norvegicus"; ?>Sarcophilus harrisii"; ?>Sus scrofa"; ?>Taeniopygia guttata"; ?>Takifugu rubripes"; ?>Tetraodon nigroviridis"; ?>Xenopus tropicalis"; ?>Xiphophorus maculatus"; ?>

Gene Ontology

GO:0005886	; C:plasma membrane
GO:0005524	; F:ATP binding
GO:0042801	; F:polo kinase kinase activity
GO:0042803	; F:protein homodimerization activity
GO:0007049	; P:cell cycle
GO:0071593	; P:lymphocyte aggregation
GO:0046777	; P:protein autophosphorylation
GO:2000401	; P:regulation of lymphocyte migration

KEGG

hsa:6793

;

InterPros

IPR011009	; Kinase-like_dom.
IPR022165	; PKK.
IPR000719	; Prot_kinase_cat_dom.
IPR017441	; Protein_kinase_ATP_BS.
IPR002290	; Ser/Thr_dual-sp_kinase_dom.
IPR008271	; Ser/Thr_kinase_AS.

Pfam

PF00069	; Pkinase; 1.
PF12474	; PKK; 2.

SMARTs

SM00220

; S_TKc; 1.

Prosites

PS00107	; PROTEIN_KINASE_ATP; 1.
PS50011	; PROTEIN_KINASE_DOM; 1.
PS00108	; PROTEIN_KINASE_ST; 1.

Prints

Created Date

20-Feb-2013