EPS-HOS-00024

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EPS-HOS-00024

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
PPM/PP2C	210.9	1.4E-61	663	908	246

Status

Reviewed

Ensembl Protein

ENSP00000383170

UniProt Accession

O60346; A1A4F5; Q641Q7; Q6P4C4; Q6PJI6; Q86TN6; Q96FK2; Q9NUY1;

Protein Name

PH domain leucine-rich repeat-containing protein phosphatase 1

Protein Synonyms/Alias

Pleckstrin homology domain-containing family E member 1; PH domain-containing family E member 1; Suprachiasmatic nucleus circadian oscillatory protein; hSCOP;

Gene Name

PHLPP1

Gene Synonyms/Alias

PHLPP1; KIAA0606, PHLPP, PLEKHE1, SCOP;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
ENSG00000081913	ENSP00000262719	ENST00000262719
ENSG00000081913	ENSP00000383170	ENST00000400316
ENSG00000081913	ENSP00000467279	ENST00000591386

Organism

Homo sapiens

Functional Description

Protein phosphatase that mediates dephosphorylation of'Ser-473' of AKT1, 'Ser-660' of PRKCB isoform beta-II and 'Ser- 657' of PRKCA. AKT1 regulates the balance between cell survival and apoptosis through a cascade that primarily alters the function of transcription factors that regulate pro- and antiapoptotic genes. Dephosphorylation of 'Ser-473' of AKT1 triggers apoptosis and suppression of tumor growth. Controls the phosphorylation of AKT2 and AKT3 more efficiently than that of AKT1. Dephosphorylation of PRKCA and PRKCB leads to their destabilization and degradation. Inhibits cancer cell proliferation and may act as a tumor suppressor. May act as a negative regulator of K-Ras signaling in membrane rafts.

Protein Length

1205

Protein Sequence
(FASTA)

MDSEIGCLIR FYAGKPHSTG SSERIQLSGM YNVRKGKMQL PVNRWTRRQV ILCGTCLIVS 60

SVKDSLTGKM HVLPLIGGKV EEVKKHQHCL AFSSSGPQSQ TYYICFDTFT EYLRWLRQVS 120

KVASQRISSV DLSCCSLEHL PANLFYSQDL THLNLKQNFL RQNPSLPAAR GLNELQRFTK 180

LKSLNLSNNH LGDFPLAVCS IPTLAELNVS CNALRSVPAA VGVMHNLQTF LLDGNFLQSL 240

PAELENMKQL SYLGLSFNEF TDIPEVLEKL TAVDKLCMSG NCVETLRLQA LRKMPHIKHV 300

DLRLNVIRKL IADEVDFLQH VTQLDLRDNK LGDLDAMIFN NIEVLHCERN QLVTLDICGY 360

FLKALYASSN ELVQLDVYPV PNYLSYMDVS RNRLENVPEW VCESRKLEVL DIGHNQICEL 420

PARLFCNSSL RKLLAGHNQL ARLPERLERT SVEVLDVQHN QLLELPPNLL MKADSLRFLN 480

ASANKLESLP PATLSEETNS ILQELYLTNN SLTDKCVPLL TGHPHLKILH MAYNRLQSFP 540

ASKMAKLEEL EEIDLSGNKL KAIPTTIMNC RRMHTVIAHS NCIEVFPEVM QLPEIKCVDL 600

SCNELSEVTL PENLPPKLQE LDLTGNPRLV LDHKTLELLN NIRCFKIDQP STGDASGAPA 660

VWSHGYTEAS GVKNKLCVAA LSVNNFCDNR EALYGVFDGD RNVEVPYLLQ CTMSDILAEE 720

LQKTKNEEEY MVNTFIVMQR KLGTAGQKLG GAAVLCHIKH DPVDPGGSFT LTSANVGKCQ 780

TVLCRNGKPL PLSRSYIMSC EEELKRIKQH KAIITEDGKV NGVTESTRIL GYTFLHPSVV 840

PRPHVQSVLL TPQDEFFILG SKGLWDSLSV EEAVEAVRNV PDALAAAKKL CTLAQSYGCH 900

DSISAVVVQL SVTEDSFCCC ELSAGGAVPP PSPGIFPPSV NMVIKDRPSD GLGVPSSSSG 960

MASEISSELS TSEMSSEVGS TASDEPPPGA LSENSPAYPS EQRCMLHPIC LSNSFQRQLS 1020

SATFSSAFSD NGLDSDDEEP IEGVFTNGSR VEVEVDIHCS RAKEKEKQQH LLQVPAEASD 1080

EGIVISANED EPGLPRKADF SAVGTIGRRR ANGSVAPQER SHNVIEVATD APLRKPGGYF 1140

AAPAQPDPDD QFIIPPELEE EVKEIMKHHQ EQQQQQQPPP PPQLQPQLPR HYQLDQLPDY 1200

YDTPL 1205

Nucleotide Sequence
(FASTA)

ATGGACTCGG AGATTGGCTG CCTCATCCGC TTCTATGCAG GAAAACCTCA CAGCACGGGT 60

AGCTCTGAAC GGATTCAGCT CTCAGGAATG TATAATGTCC GTAAAGGCAA GATGCAGTTG 120

CCAGTGAACC GATGGACAAG ACGCCAAGTC ATCCTATGTG GGACCTGCCT GATAGTATCA 180

TCTGTGAAAG ACAGCTTGAC CGGAAAGATG CATGTTCTGC CACTAATTGG TGGAAAAGTA 240

GAAGAAGTGA AAAAGCACCA ACACTGTTTA GCATTTAGCT CCTCTGGACC CCAAAGCCAG 300

ACTTACTACA TTTGCTTTGA TACTTTCACA GAATACTTAA GGTGGCTGCG ACAAGTCTCC 360

AAGGTTGCAT CCCAGCGCAT TAGCTCAGTG GACCTCTCGT GTTGTAGCCT GGAACATCTG 420

CCTGCCAACC TCTTCTACAG CCAAGACCTC ACTCATCTCA ATTTAAAACA AAACTTCCTA 480

AGGCAGAACC CTAGCCTTCC AGCTGCCAGG GGGCTTAATG AACTGCAAAG GTTCACCAAG 540

TTGAAGAGTC TTAACCTTTC CAATAATCAT TTAGGGGACT TCCCACTGGC AGTCTGCAGT 600

ATTCCAACCC TGGCAGAGCT GAACGTGTCC TGCAATGCCC TGCGATCAGT CCCGGCAGCC 660

GTTGGAGTGA TGCACAACTT ACAGACATTT TTGTTGGATG GAAACTTTCT CCAATCCCTT 720

CCTGCTGAGT TGGAGAACAT GAAGCAGCTT AGTTATCTGG GTCTTTCTTT CAATGAATTT 780

ACTGACATTC CCGAAGTATT GGAGAAATTG ACTGCTGTGG ATAAACTTTG TATGTCTGGA 840

AACTGTGTGG AGACCCTTAG GCTACAGGCT TTAAGAAAAA TGCCTCACAT TAAACATGTG 900

GATCTAAGGT TGAACGTAAT TAGGAAGCTG ATAGCAGATG AAGTGGACTT TCTACAGCAT 960

GTTACTCAGC TTGACCTACG AGACAATAAG CTTGGTGATC TAGATGCTAT GATTTTCAAC 1020

AACATTGAAG TTTTACACTG TGAAAGGAAT CAACTGGTCA CATTAGACAT CTGTGGCTAT 1080

TTCCTAAAAG CGCTCTATGC CTCTTCTAAT GAACTTGTTC AACTTGATGT TTACCCAGTT 1140

CCAAATTATC TGTCCTACAT GGATGTTTCA AGGAACCGCT TAGAAAATGT GCCTGAGTGG 1200

GTATGTGAAA GCCGAAAGCT AGAAGTTTTG GATATTGGCC ATAATCAAAT ATGTGAACTT 1260

CCTGCCCGCT TATTTTGTAA TAGCAGTCTC CGGAAACTAC TGGCAGGACA CAACCAGTTG 1320

GCAAGGCTGC CTGAAAGGCT AGAAAGAACC TCGGTGGAGG TCTTGGATGT GCAACACAAC 1380

CAGCTCCTTG AGCTCCCACC TAACCTTCTG ATGAAGGCTG ACAGCCTGAG ATTCCTGAAC 1440

GCCTCTGCGA ACAAACTGGA AAGCCTTCCT CCAGCCACGC TTTCCGAAGA GACAAACAGT 1500

ATCTTACAAG AGTTGTATTT GACAAATAAC AGCCTCACAG ACAAATGTGT GCCCTTGTTA 1560

ACGGGACACC CCCATTTGAA GATCCTTCAC ATGGCCTATA ACCGACTTCA GAGTTTTCCA 1620

GCAAGTAAAA TGGCGAAACT GGAGGAACTT GAAGAAATTG ATCTCAGTGG GAATAAGCTG 1680

AAAGCCATCC CAACAACGAT CATGAATTGC AGGCGCATGC ACACCGTGAT TGCTCACTCC 1740

AACTGCATCG AGGTCTTTCC CGAAGTTATG CAGCTCCCAG AGATCAAGTG TGTGGACCTG 1800

AGCTGTAATG AGCTAAGTGA AGTCACATTA CCAGAAAACC TGCCTCCCAA ACTGCAGGAG 1860

CTAGACCTGA CTGGAAACCC GCGCCTTGTC CTTGATCACA AAACCCTGGA ACTACTGAAT 1920

AATATCCGCT GTTTCAAGAT TGATCAGCCT TCTACAGGAG ACGCTTCCGG AGCCCCAGCT 1980

GTATGGAGTC ATGGTTACAC TGAAGCTTCG GGGGTAAAAA ACAAGTTGTG TGTCGCAGCC 2040

CTGTCGGTGA ATAACTTCTG TGACAACCGC GAAGCCCTGT ATGGTGTGTT TGACGGAGAC 2100

CGGAATGTGG AGGTGCCCTA CCTTCTCCAG TGCACTATGA GTGACATTTT GGCTGAAGAG 2160

CTGCAAAAAA CAAAAAACGA AGAAGAATAC ATGGTCAATA CATTCATTGT CATGCAAAGG 2220

AAACTTGGAA CTGCTGGGCA GAAGCTTGGT GGTGCCGCTG TCCTTTGTCA TATCAAGCAT 2280

GACCCTGTGG ATCCAGGAGG ATCCTTCACC TTGACCTCTG CTAATGTGGG CAAGTGCCAA 2340

ACAGTTCTCT GTCGAAATGG AAAGCCGCTG CCTCTGTCCA GATCTTACAT CATGAGCTGT 2400

GAAGAAGAGC TGAAGAGGAT TAAACAGCAC AAGGCCATTA TCACTGAGGA TGGCAAGGTG 2460

AACGGAGTGA CTGAGTCCAC GCGCATCCTG GGCTACACCT TCCTCCATCC CAGTGTGGTG 2520

CCTCGCCCCC ACGTGCAGTC CGTGCTCCTG ACTCCCCAGG ATGAGTTCTT CATCCTAGGC 2580

AGTAAGGGGT TGTGGGACAG CCTGTCCGTC GAGGAGGCCG TGGAAGCCGT GCGCAACGTG 2640

CCCGATGCCC TGGCTGCTGC CAAGAAGCTG TGTACCCTGG CCCAGAGCTA CGGCTGCCAC 2700

GACAGCATCA GCGCTGTGGT GGTGCAGCTC AGTGTCACTG AGGACAGCTT CTGCTGCTGC 2760

GAGCTCAGCG CCGGTGGGGC TGTGCCACCA CCCAGTCCTG GCATCTTTCC TCCCTCAGTG 2820

AACATGGTGA TCAAGGATCG GCCCTCAGAT GGGCTGGGCG TGCCGTCCTC CAGCAGCGGC 2880

ATGGCTTCCG AGATTAGCAG TGAGCTCTCC ACTTCTGAGA TGAGCAGCGA GGTGGGGTCA 2940

ACAGCCTCCG ATGAGCCCCC GCCCGGAGCC CTAAGCGAGA ACAGCCCTGC CTACCCCAGT 3000

GAGCAGCGCT GCATGCTCCA CCCCATCTGT CTGTCCAACT CCTTCCAGCG CCAGCTATCC 3060

AGCGCCACGT TCTCTAGCGC CTTCTCCGAC AACGGCCTTG ACAGTGACGA TGAGGAGCCC 3120

ATCGAGGGCG TCTTCACCAA CGGCAGCCGG GTGGAGGTGG AGGTGGACAT CCACTGCAGC 3180

CGGGCCAAGG AGAAGGAGAA ACAGCAGCAC CTGCTTCAGG TGCCAGCAGA GGCCAGTGAT 3240

GAGGGCATTG TCATCAGCGC CAACGAGGAT GAGCCAGGTC TGCCCAGGAA GGCAGACTTC 3300

TCTGCCGTTG GGACCATTGG GCGCCGGAGG GCCAATGGCT CTGTTGCGCC CCAGGAAAGG 3360

AGCCACAATG TGATAGAGGT GGCTACAGAC GCACCTCTTC GAAAGCCTGG AGGCTATTTT 3420

GCTGCCCCGG CTCAGCCGGA TCCTGATGAT CAGTTTATCA TACCCCCGGA GCTGGAAGAG 3480

GAGGTCAAAG AAATCATGAA GCATCACCAG GAGCAACAGC AGCAGCAGCA GCCGCCACCA 3540

CCCCCTCAGC TCCAGCCGCA GCTGCCGCGG CACTACCAGC TGGACCAGCT GCCAGATTAT 3600

TACGACACGC CACTATGA 3618

Domain Profile

S: 2     lvgvvsvngrrnemedavtsspnflkspekkdrlaffgvyDGhggsevakfcseklheil  61

         ++g+++ +g +n+++ a+ s++nf++++e+     ++gv+DG+++ ev + +++++++il

Q: 663   SHGYTEASGVKNKLCVAALSVNNFCDNREA-----LYGVFDGDRNVEVPYLLQCTMSDIL  717

         689***********************9885.....9************************

S: 62    aeelesseseerkvkkalakaFlstdeelkvleeksldidksGstavvalikds......  115

         aeel+    ++++ ++++ ++F+ ++++l       ++ +k G +av+++ik++

Q: 718   AEELQ----KTKNEEEYMVNTFIVMQRKL------GTAGQKLGGAAVLCHIKHDpvdpgg  767

         ***99....999*****************......99***********************

S: 116   ..klyvanvGdSravLcrngkavelssdHkpsredEleRieeaggsvilkdgaRvlGvLa  173

           +l+ anvG++++vLcrngk+++ls+ + +s+e+El+Ri+++++ ++++   +v+Gv++

Q: 768   sfTLTSANVGKCQTVLCRNGKPLPLSRSYIMSCEEELKRIKQHKAIITEDG--KVNGVTE  825

         *99*****************************************9999998..*******

S: 174   vsRalGdlylkpyvtpePevtshrltsedefliLasDGlwdvvsneeavslvrdvlekke  233

          +R lG ++l+p v+p+P+v+s+ lt++def+iL+s+Glwd +s eeav++vr+v +

Q: 826   STRILGYTFLHPSVVPRPHVQSVLLTPQDEFFILGSKGLWDSLSVEEAVEAVRNVPD---  882

         *********************************************************...

S: 234   aeqeaakkLvklalargskdnvsaivv  260

          + +aakkL++la+++g++d++sa+vv

Q: 883   -ALAAAKKLCTLAQSYGCHDSISAVVV  908

         .************************97

Domain Sequence
(FASTA)

SHGYTEASGV KNKLCVAALS VNNFCDNREA LYGVFDGDRN VEVPYLLQCT MSDILAEELQ 60

KTKNEEEYMV NTFIVMQRKL GTAGQKLGGA AVLCHIKHDP VDPGGSFTLT SANVGKCQTV 120

LCRNGKPLPL SRSYIMSCEE ELKRIKQHKA IITEDGKVNG VTESTRILGY TFLHPSVVPR 180

PHVQSVLLTP QDEFFILGSK GLWDSLSVEE AVEAVRNVPD ALAAAKKLCT LAQSYGCHDS 240

ISAVVV 246

Keyword

Apoptosis; Complete proteome; Cytoplasm; Hydrolase; Leucine-rich repeat; Manganese; Membrane; Metal-binding; Nucleus; Phosphoprotein; Polymorphism; Protein phosphatase; Reference proteome; Repeat; Tumor suppressor.

Sequence Source

Ensembl

Orthology

Ortholog group

Ailuropoda melanoleuca"; ?>Anolis carolinensis"; ?>Bos taurus"; ?>Caenorhabditis elegans"; ?>Callithrix jacchus"; ?>Canis familiaris"; ?>Cavia porcellus"; ?>Danio rerio"; ?>Equus caballus"; ?>Felis catus"; ?>Gadus morhua"; ?>Gallus gallus"; ?>Gasterosteus aculeatus"; ?>Gorilla gorilla"; ?>Loxodonta africana"; ?>Meleagris gallopavo"; ?>Monodelphis domestica"; ?>Mus musculus"; ?>Myotis lucifugus"; ?>Nomascus leucogenys"; ?>Ochotona princeps"; ?>Oreochromis niloticus"; ?>Oryctolagus cuniculus"; ?>Oryzias latipes"; ?>Otolemur garnettii"; ?>Pan troglodytes"; ?>Pelodiscus sinensis"; ?>Petromyzon marinus"; ?>Pongo abelii"; ?>Rattus norvegicus"; ?>Sarcophilus harrisii"; ?>Sus scrofa"; ?>Taeniopygia guttata"; ?>Takifugu rubripes"; ?>Tetraodon nigroviridis"; ?>Tursiops truncatus"; ?>Xenopus tropicalis"; ?>Xiphophorus maculatus"; ?>Saccharomyces cerevisiae"; ?>Schizosaccharomyces pombe"; ?>

Gene Ontology

GO:0005829	; C:cytosol
GO:0016020	; C:membrane
GO:0005634	; C:nucleus
GO:0046872	; F:metal ion binding
GO:0005543	; F:phospholipid binding
GO:0004722	; F:protein serine/threonine phosphatase activity
GO:0006915	; P:apoptotic process
GO:0007623	; P:circadian rhythm
GO:0009649	; P:entrainment of circadian clock
GO:0007173	; P:epidermal growth factor receptor signaling pathway
GO:0008543	; P:fibroblast growth factor receptor signaling pathway
GO:0048011	; P:nerve growth factor receptor signaling pathway
GO:0048015	; P:phosphatidylinositol-mediated signaling

KEGG

hsa:23239

;

InterPros

IPR001611	; Leu-rich_rpt.
IPR011993	; PH_like_dom.
IPR001849	; Pleckstrin_homology.
IPR001932	; PP2C-like.

Pfam

PF00560	; LRR_1; 2.
PF00481	; PP2C; 1.

SMARTs

SM00233	; PH; 1.
SM00332	; PP2Cc; 1.

Prosites

PS51450	; LRR; 17.
PS50003	; PH_DOMAIN; 1.

Prints

Created Date

20-Feb-2013