EKS-HOS-00439

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EKS-HOS-00439

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
Other/ULK	394.7	1.1E-117	9	271	263

Status

Reviewed

Ensembl Protein

ENSP00000354877

UniProt Accession

Q8IYT8; A8MY69; O75119;

Protein Name

Serine/threonine-protein kinase ULK2

Protein Synonyms/Alias

Unc-51-like kinase 2;

Gene Name

ULK2

Gene Synonyms/Alias

ULK2; KIAA0623;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
ENSG00000083290	ENSP00000378914	ENST00000395544
ENSG00000083290	ENSP00000467277	ENST00000580118
ENSG00000083290	ENSP00000465666	ENST00000574732
ENSG00000083290	ENSP00000354877	ENST00000361658
ENSG00000083290	ENSP00000464892	ENST00000575432

Organism

Homo sapiens

Functional Description

Serine/threonine-protein kinase involved in autophagy inresponse to starvation. Acts upstream of phosphatidylinositol 3- kinase PIK3C3 to regulate the formation of autophagophores, the precursors of autophagosomes. Part of regulatory feedback loops in autophagy: acts both as a downstream effector and a negative regulator of mammalian target of rapamycin complex 1 (mTORC1) via interaction with RPTOR. Activated via phosphorylation by AMPK, also acts as a negative regulator of AMPK through phosphorylation of the AMPK subunits PRKAA1, PRKAB2 and PRKAG1. May phosphorylate ATG13/KIAA0652, FRS2, FRS3 and RPTOR; however such data need additional evidences. Not involved in ammonia-induced autophagy or in autophagic response of cerebellar granule neurons (CGN) to low potassium concentration. Plays a role early in neuronal differentiation and is required for granule cell axon formation: may govern axon formation via Ras-like GTPase signaling and through regulation of the Rab5-mediated endocytic pathways within developing axons.

Protein Length

1036

Protein Sequence
(FASTA)

MEVVGDFEYS KRDLVGHGAF AVVFRGRHRQ KTDWEVAIKS INKKNLSKSQ ILLGKEIKIL 60

KELQHENIVA LYDVQELPNS VFLVMEYCNG GDLADYLQAK GTLSEDTIRV FLHQIAAAMR 120

ILHSKGIIHR DLKPQNILLS YANRRKSSVS GIRIKIADFG FARYLHSNMM AATLCGSPMY 180

MAPEVIMSQH YDAKADLWSI GTVIYQCLVG KPPFQANSPQ DLRMFYEKNR SLMPSIPRET 240

SPYLANLLLG LLQRNQKDRM DFEAFFSHPF LEQGPVKKSC PVPVPMYSGS VSGSSCGSSP 300

SCRFASPPSL PDMQHIQEEN LSSPPLGPPN YLQVSKDSAS TSSKNSSCDT DDFVLVPHNI 360

SSDHSCDMPV GTAGRRASNE FLVCGGQCQP TVSPHSETAP IPVPTQIRNY QRIEQNLTST 420

ASSGTNVHGS PRSAVVRRSN TSPMGFLRPG SCSPVPADTA QTVGRRLSTG SSRPYSPSPL 480

VGTIPEQFSQ CCCGHPQGHD SRSRNSSGSP VPQAQSPQSL LSGARLQSAP TLTDIYQNKQ 540

KLRKQHSDPV CPSHTGAGYS YSPQPSRPGS LGTSPTKHLG SSPRSSDWFF KTPLPTIIGS 600

PTKTTAPFKI PKTQASSNLL ALVTRHGPAE EQSKDGNEPR ECAHCLLVQG SERQRAEQQS 660

KAVFGRSVST GKLSDQQGKT PICRHQGSTD SLNTERPMDI APAGACGGVL APPAGTAASS 720

KAVLFTVGSP PHSAAAPTCT HMFLRTRTTS VGPSNSGGSL CAMSGRVCVG SPPGPGFGSS 780

PPGAEAAPSL RYVPYGASPP SLEGLITFEA PELPEETLME REHTDTLRHL NVMLMFTECV 840

LDLTAMRGGN PELCTSAVSL YQIQESVVVD QISQLSKDWG RVEQLVLYMK AAQLLAASLH 900

LAKAQIKSGK LSPSTAVKQV VKNLNERYKF CITMCKKLTE KLNRFFSDKQ RFIDEINSVT 960

AEKLIYNCAV EMVQSAALDE MFQQTEDIVY RYHKAALLLE GLSRILQDPA DIENVHKYKC 1020

SIERRLSALC HSTATV 1036

Nucleotide Sequence
(FASTA)

ATGGAGGTGG TGGGTGACTT CGAGTACAGC AAGAGGGATC TCGTGGGACA CGGGGCCTTC 60

GCCGTGGTCT TCCGGGGGCG GCACCGCCAG AAAACTGATT GGGAGGTAGC TATTAAAAGT 120

ATTAATAAAA AGAACTTGTC AAAATCACAA ATACTGCTTG GAAAGGAAAT TAAAATCTTA 180

AAGGAACTTC AGCATGAAAA TATTGTAGCA CTCTATGATG TTCAGGAATT ACCCAACTCT 240

GTCTTTTTGG TGATGGAGTA TTGCAATGGT GGAGACCTCG CAGATTATTT GCAAGCGAAA 300

GGGACTCTCA GTGAAGACAC GATCAGAGTG TTTCTGCATC AGATTGCTGC TGCCATGCGA 360

ATCCTGCACA GCAAAGGAAT CATCCACAGA GATCTCAAAC CACAGAACAT CTTGCTGTCC 420

TATGCCAATC GCAGAAAATC AAGTGTCAGT GGTATTCGCA TCAAAATAGC GGATTTTGGT 480

TTTGCTCGTT ACCTACATAG TAACATGATG GCTGCAACAC TGTGTGGATC CCCGATGTAC 540

ATGGCTCCTG AGGTTATTAT GTCTCAACAT TATGATGCTA AGGCTGACTT GTGGAGCATA 600

GGAACAGTGA TATACCAATG CCTAGTTGGA AAACCACCTT TTCAGGCCAA TAGTCCTCAA 660

GACTTAAGGA TGTTTTATGA AAAAAACAGG AGCTTAATGC CTAGTATTCC CAGAGAAACA 720

TCACCTTATT TGGCTAATCT CCTTTTGGGT TTGCTTCAGA GAAACCAAAA AGATAGAATG 780

GACTTTGAAG CATTTTTTAG CCATCCTTTT CTTGAGCAAG GTCCAGTAAA AAAATCTTGC 840

CCAGTTCCAG TGCCCATGTA TTCTGGTTCT GTCTCTGGAA GCTCCTGTGG CAGCTCTCCA 900

TCTTGTCGTT TTGCTTCTCC ACCATCCCTT CCAGATATGC AGCATATTCA GGAAGAAAAC 960

TTATCTTCCC CACCATTGGG TCCTCCCAAC TATCTACAAG TTTCCAAAGA TTCTGCCAGT 1020

ACTAGTAGCA AGAACTCTTC TTGTGACACG GATGACTTTG TTTTGGTGCC ACACAACATC 1080

TCGTCAGACC ACTCATGTGA TATGCCAGTG GGGACTGCTG GCAGACGTGC TTCAAATGAA 1140

TTCTTGGTGT GTGGAGGGCA GTGTCAGCCT ACTGTGTCAC CTCACAGCGA AACAGCACCA 1200

ATTCCAGTTC CTACTCAAAT AAGGAATTAT CAGCGCATAG AGCAGAATCT TACATCTACT 1260

GCCAGCTCAG GCACAAATGT ACATGGTTCT CCAAGATCTG CAGTGGTACG AAGGTCCAAC 1320

ACCAGCCCCA TGGGCTTCCT CCGGCCGGGA TCATGCTCCC CAGTACCAGC AGACACAGCA 1380

CAGACAGTTG GACGAAGGCT CTCCACTGGG TCTTCTAGGC CTTACTCACC TTCCCCTTTG 1440

GTTGGTACCA TTCCTGAGCA ATTCAGTCAG TGCTGCTGTG GGCATCCTCA GGGCCATGAC 1500

TCCAGGAGTA GAAACTCCTC AGGTTCTCCA GTGCCACAAG CTCAGTCCCC ACAGTCTCTC 1560

TTATCGGGTG CTAGACTGCA GAGCGCCCCC ACCCTCACTG ACATCTATCA GAACAAGCAG 1620

AAGCTCAGAA AACAGCACTC TGACCCCGTG TGCCCATCCC ATACTGGGGC TGGGTACAGC 1680

TACTCGCCTC AGCCCAGTCG GCCTGGCAGC CTTGGAACTT CTCCCACCAA GCACTTGGGG 1740

TCCTCTCCAC GGAGTTCTGA CTGGTTCTTT AAAACTCCTT TGCCAACAAT CATTGGCTCT 1800

CCTACTAAGA CCACAGCTCC TTTCAAAATC CCTAAAACTC AAGCATCTTC CAACCTGTTA 1860

GCCTTGGTTA CTCGTCATGG GCCTGCTGAA GAACAGTCGA AAGATGGGAA TGAGCCACGG 1920

GAATGTGCCC ATTGCCTCTT AGTGCAAGGA AGTGAGAGGC AGCGGGCCGA GCAGCAGAGC 1980

AAGGCAGTGT TTGGCAGATC TGTCAGTACC GGGAAGTTAT CAGATCAACA AGGAAAGACT 2040

CCTATATGTC GACATCAGGG CAGCACAGAC AGTTTAAATA CAGAACGACC AATGGATATA 2100

GCTCCGGCAG GAGCCTGTGG TGGTGTTCTG GCACCTCCTG CAGGTACAGC AGCAAGTTCC 2160

AAGGCTGTCC TCTTCACTGT AGGGTCTCCT CCACACAGTG CGGCAGCCCC CACTTGTACC 2220

CACATGTTCC TTCGAACAAG AACAACCTCA GTGGGGCCCA GCAACTCCGG GGGCTCTCTT 2280

TGTGCCATGA GTGGCCGCGT GTGCGTGGGG TCCCCGCCTG GCCCAGGCTT CGGCTCTTCC 2340

CCTCCAGGAG CAGAGGCAGC TCCCAGCCTG AGATACGTGC CTTACGGTGC TTCACCCCCC 2400

AGCCTAGAGG GGCTCATCAC CTTTGAAGCC CCTGAACTGC CGGAGGAGAC GCTGATGGAG 2460

CGGGAACACA CAGACACCTT ACGCCATCTG AATGTGATGC TGATGTTCAC TGAGTGTGTG 2520

CTGGACCTGA CAGCCATGAG GGGAGGAAAC CCTGAGCTGT GCACATCTGC TGTGTCCTTG 2580

TACCAGATCC AGGAGAGTGT GGTGGTGGAC CAGATCAGTC AGCTGAGCAA AGACTGGGGG 2640

CGGGTGGAGC AGCTGGTGTT GTACATGAAA GCAGCACAGC TGCTTGCGGC TTCTCTGCAT 2700

CTTGCCAAAG CCCAGATCAA GTCCGGGAAA CTGAGCCCAT CCACAGCTGT GAAACAAGTT 2760

GTCAAGAATC TGAACGAACG ATATAAATTC TGCATCACCA TGTGCAAGAA ACTTACAGAA 2820

AAGCTGAATC GATTCTTCTC TGACAAACAG AGGTTTATTG ATGAAATCAA CAGTGTGACT 2880

GCAGAGAAAC TCATCTATAA TTGTGCTGTA GAAATGGTTC AGTCTGCAGC CCTGGATGAG 2940

ATGTTTCAGC AGACCGAAGA TATTGTTTAT CGCTATCATA AGGCAGCCCT TCTTTTGGAA 3000

GGCCTAAGTA GGATTCTACA GGACCCTGCA GATATTGAAA ATGTGCATAA ATATAAATGT 3060

AGTATTGAGA GAAGACTGTC GGCGCTCTGC CATAGCACCG CAACCGTGTG A 3111

Domain Profile

S: 1     ysleeeiGkGsfavvykgrlrkktsqvvAikvvekkklkkslenlekeikilkelkheni  60

         ys+++ +G+G+favv++gr+r+kt+++vAik+++kk+l+ks+  l keikilkel+heni

Q: 9     YSKRDLVGHGAFAVVFRGRHRQKTDWEVAIKSINKKNLSKSQILLGKEIKILKELQHENI  68

         8899********************************************************

S: 61    valldveetskevylvmeycaggdLakllkqkkeLsedtvreflqqlasalkaLhskkiv  120

         val+dv+e  ++v+lvmeyc+ggdLa++l++k++Lsedt+r+fl+q+a+a+++Lhsk+i+

Q: 69    VALYDVQELPNSVFLVMEYCNGGDLADYLQAKGTLSEDTIRVFLHQIAAAMRILHSKGII  128

         ************************************************************

S: 121   hrDlkPqniLlsyadakk.elkkvtlkladFglarvlkeesmaatlkGsplYmaPEvvke  179

         hrDlkPqniLlsya+++k +++ +++k+adFg+ar+l++++maatl+Gsp+YmaPEv+++

Q: 129   HRDLKPQNILLSYANRRKsSVSGIRIKIADFGFARYLHSNMMAATLCGSPMYMAPEVIMS  188

         ***************99989****************************************

S: 180   qqydakaDlWSiGtilyellvGkpPfaaesiseLvekiekndvleppipkeisselkdll  239

         q+ydakaDlWSiGt++y++lvGkpPf+a+s+++L++++ekn++l+p+ip+e+s+ l++ll

Q: 189   QHYDAKADLWSIGTVIYQCLVGKPPFQANSPQDLRMFYEKNRSLMPSIPRETSPYLANLL  248

         ************************************************************

S: 240   qsLLqrdpkkRisfeelfahpfl  262

          +LLqr++k+R++fe +f+hpfl

Q: 249   LGLLQRNQKDRMDFEAFFSHPFL  271

         *********************96

Domain Sequence
(FASTA)

YSKRDLVGHG AFAVVFRGRH RQKTDWEVAI KSINKKNLSK SQILLGKEIK ILKELQHENI 60

VALYDVQELP NSVFLVMEYC NGGDLADYLQ AKGTLSEDTI RVFLHQIAAA MRILHSKGII 120

HRDLKPQNIL LSYANRRKSS VSGIRIKIAD FGFARYLHSN MMAATLCGSP MYMAPEVIMS 180

QHYDAKADLW SIGTVIYQCL VGKPPFQANS PQDLRMFYEK NRSLMPSIPR ETSPYLANLL 240

LGLLQRNQKD RMDFEAFFSH PFL 263

Keyword

ATP-binding; Autophagy; Complete proteome; Cytoplasmic vesicle; Kinase; Membrane; Neurogenesis; Nucleotide-binding; Phosphoprotein; Polymorphism; Reference proteome; Serine/threonine-protein kinase; Transferase.

Sequence Source

Ensembl

Orthology

Ortholog group

Ailuropoda melanoleuca"; ?>Bos taurus"; ?>Caenorhabditis elegans"; ?>Callithrix jacchus"; ?>Canis familiaris"; ?>Cavia porcellus"; ?>Equus caballus"; ?>Felis catus"; ?>Gadus morhua"; ?>Gallus gallus"; ?>Gasterosteus aculeatus"; ?>Gorilla gorilla"; ?>Ictidomys tridecemlineatus"; ?>Latimeria chalumnae"; ?>Loxodonta africana"; ?>Macaca mulatta"; ?>Monodelphis domestica"; ?>Mus musculus"; ?>Mustela putorius furo"; ?>Myotis lucifugus"; ?>Nomascus leucogenys"; ?>Oreochromis niloticus"; ?>Ornithorhynchus anatinus"; ?>Oryctolagus cuniculus"; ?>Oryzias latipes"; ?>Otolemur garnettii"; ?>Pan troglodytes"; ?>Pelodiscus sinensis"; ?>Pongo abelii"; ?>Rattus norvegicus"; ?>Sarcophilus harrisii"; ?>Taeniopygia guttata"; ?>Takifugu rubripes"; ?>Tetraodon nigroviridis"; ?>Xenopus tropicalis"; ?>Schizosaccharomyces pombe"; ?>

Gene Ontology

GO:0030659	; C:cytoplasmic vesicle membrane
GO:0034045	; C:pre-autophagosomal structure membrane
GO:0005524	; F:ATP binding
GO:0004674	; F:protein serine/threonine kinase activity
GO:0006914	; P:autophagy
GO:0048675	; P:axon extension
GO:0048671	; P:negative regulation of collateral sprouting
GO:0046777	; P:protein autophosphorylation
GO:0010506	; P:regulation of autophagy
GO:0042594	; P:response to starvation
GO:0007165	; P:signal transduction

KEGG

hsa:9706

;

InterPros

IPR011009	; Kinase-like_dom.
IPR000719	; Prot_kinase_cat_dom.
IPR017441	; Protein_kinase_ATP_BS.
IPR002290	; Ser/Thr_dual-sp_kinase_dom.
IPR016237	; Ser/Thr_kin_STPK_Ulk-1/2.
IPR008271	; Ser/Thr_kinase_AS.
IPR022708	; Ser/Thr_kinase_C.

Pfam

PF12063	; DUF3543; 1.
PF00069	; Pkinase; 1.

SMARTs

SM00220

; S_TKc; 1.

Prosites

PS00107	; PROTEIN_KINASE_ATP; 1.
PS50011	; PROTEIN_KINASE_DOM; 1.
PS00108	; PROTEIN_KINASE_ST; 1.

Prints

Created Date

20-Feb-2013