EKS-HOS-00397

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EKS-HOS-00397

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
STE/STE11	348.8	1.2E-103	1343	1601	259

Status

Reviewed

Ensembl Protein

ENSP00000375986

UniProt Accession

Q9Y6R4; A6H8W0; B7ZLD3; B9EG75; Q5VTT8; Q5VTT9; Q92612; Q9H408;

Protein Name

Mitogen-activated protein kinase kinase kinase 4

Protein Synonyms/Alias

MAP three kinase 1; MAPK/ERK kinase kinase 4; MEK kinase 4; MEKK 4;

Gene Name

MAP3K4

Gene Synonyms/Alias

MAP3K4; KIAA0213, MAPKKK4, MEKK4, MTK1;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
ENSG00000085511	ENSP00000355886	ENST00000366919
ENSG00000085511	ENSP00000446303	ENST00000490904
ENSG00000085511	ENSP00000439377	ENST00000542952
ENSG00000085511	ENSP00000355887	ENST00000366920
ENSG00000085511	ENSP00000297332	ENST00000348824
ENSG00000085511	ENSP00000375986	ENST00000392142
ENSG00000085511	ENSP00000445018	ENST00000544041
ENSG00000085511	ENSP00000390508	ENST00000448119

Organism

Homo sapiens

Functional Description

Component of a protein kinase signal transductioncascade. Activates the CSBP2, P38 and JNK MAPK pathways, but not the ERK pathway. Specifically phosphorylates and activates MAP2K4 and MAP2K6.

Protein Length

1608

Protein Sequence
(FASTA)

MREAAAALVP PPAFAVTPAA AMEEPPPPPP PPPPPPEPET ESEPECCLAA RQEGTLGDSA 60

CKSPESDLED FSDETNTENL YGTSPPSTPR QMKRMSTKHQ RNNVGRPASR SNLKEKMNAP 120

NQPPHKDTGK TVENVEEYSY KQEKKIRAAL RTTERDRKKN VQCSFMLDSV GGSLPKKSIP 180

DVDLNKPYLS LGCSNAKLPV SVPMPIARPA RQTSRTDCPA DRLKFFETLR LLLKLTSVSK 240

KKDREQRGQE NTSGFWLNRS NELIWLELQA WHAGRTINDQ DFFLYTARQA IPDIINEILT 300

FKVDYGSFAF VRDRAGFNGT SVEGQCKATP GTKIVGYSTH HEHLQRQRVS FEQVKRIMEL 360

LEYIEALYPS LQALQKDYEK YAAKDFQDRV QALCLWLNIT KDLNQKLRIM GTVLGIKNLS 420

DIGWPVFEIP SPRPSKGNEP EYEGDDTEGE LKELESSTDE SEEEQISDPR VPEIRQPIDN 480

SFDIQSRDCI SKKLERLESE DDSLGWGAPD WSTEAGFSRH CLTSIYRPFV DKALKQMGLR 540

KLILRLHKLM DGSLQRARIA LVKNDRPVEF SEFPDPMWGS DYVQLSRTPP SSEEKCSAVS 600

WEELKAMDLP SFEPAFLVLC RVLLNVIHEC LKLRLEQRPA GEPSLLSIKQ LVRECKEVLK 660

GGLLMKQYYQ FMLQEVLEDL EKPDCNIDAF EEDLHKMLMV YFDYMRSWIQ MLQQLPQASH 720

SLKNLLEEEW NFTKEITHYI RGGEAQAGKL FCDIAGMLLK STGSFLEFGL QESCAEFWTS 780

ADDSSASDEI RRSVIEISRA LKELFHEARE RASKALGFAK MLRKDLEIAA EFRLSAPVRD 840

LLDVLKSKQY VKVQIPGLEN LQMFVPDTLA EEKSIILQLL NAAAGKDCSK DSDDVLIDAY 900

LLLTKHGDRA RDSEDSWGTW EAQPVKVVPQ VETVDTLRSM QVDNLLLVVM QSAHLTIQRK 960

AFQQSIEGLM TLCQEQTSSQ PVIAKALQQL KNDALELCNR ISNAIDRVDH MFTSEFDAEV 1020

DESESVTLQQ YYREAMIQGY NFGFEYHKEV VRLMSGEFRQ KIGDKYISFA RKWMNYVLTK 1080

CESGRGTRPR WATQGFDFLQ AIEPAFISAL PEDDFLSLQA LMNECIGHVI GKPHSPVTGL 1140

YLAIHRNSPR PMKVPRCHSD PPNPHLIIPT PEGFSTRSMP SDARSHGSPA AAAAAAAAAV 1200

AASRPSPSGG DSVLPKSISS AHDTRGSSVP ENDRLASIAA ELQFRSLSRH SSPTEERDEP 1260

AYPRGDSSGS TRRSWELRTL ISQSKDTASK LGPIEAIQKS VRLFEEKRYR EMRRKNIIGQ 1320

VCDTPKSYDN VMHVGLRKVT FKWQRGNKIG EGQYGKVYTC ISVDTGELMA MKEIRFQPND 1380

HKTIKETADE LKIFEGIKHP NLVRYFGVEL HREEMYIFME YCDEGTLEEV SRLGLQEHVI 1440

RLYSKQITIA INVLHEHGIV HRDIKGANIF LTSSGLIKLG DFGCSVKLKN NAQTMPGEVN 1500

STLGTAAYMA PEVITRAKGE GHGRAADIWS LGCVVIEMVT GKRPWHEYEH NFQIMYKVGM 1560

GHKPPIPERL SPEGKDFLSH CLESDPKMRW TASQLLDHSF VKVCTDEE 1608

Nucleotide Sequence
(FASTA)

ATGAGAGAAG CCGCTGCCGC GCTGGTCCCT CCTCCCGCCT TTGCCGTCAC GCCTGCCGCC 60

GCCATGGAGG AGCCGCCGCC ACCGCCGCCG CCGCCACCAC CGCCACCGGA ACCCGAGACC 120

GAGTCAGAAC CCGAGTGCTG CTTGGCGGCG AGGCAAGAGG GCACATTGGG AGATTCAGCT 180

TGCAAGAGTC CTGAATCTGA TCTAGAAGAC TTCTCCGATG AAACAAATAC AGAGAATCTT 240

TATGGTACCT CTCCCCCCAG CACACCTCGA CAGATGAAAC GCATGTCAAC CAAACATCAG 300

AGGAATAATG TGGGGAGGCC AGCCAGTCGG TCTAATTTGA AAGAAAAAAT GAATGCACCA 360

AATCAGCCTC CACATAAAGA CACTGGAAAA ACAGTGGAGA ATGTGGAAGA ATACAGCTAT 420

AAGCAGGAGA AAAAGATCCG AGCAGCTCTT AGAACAACAG AGCGTGATCG TAAAAAAAAT 480

GTACAGTGCT CATTCATGTT AGACTCAGTG GGTGGATCTT TGCCAAAAAA ATCAATTCCA 540

GATGTGGATC TCAATAAGCC TTACCTCAGC CTTGGCTGTA GCAATGCTAA GCTTCCAGTA 600

TCTGTGCCCA TGCCTATAGC CAGACCTGCA CGCCAGACTT CTAGGACTGA CTGTCCAGCA 660

GATCGTTTAA AGTTTTTTGA AACTTTACGA CTTTTGCTAA AGCTTACCTC AGTCTCAAAG 720

AAAAAAGACA GGGAGCAAAG AGGACAAGAA AATACGTCTG GTTTCTGGCT TAACCGATCT 780

AACGAACTGA TCTGGTTAGA GCTACAAGCC TGGCATGCAG GACGGACAAT TAACGACCAG 840

GACTTCTTTT TATATACAGC CCGTCAAGCC ATCCCAGATA TTATTAATGA AATCCTTACT 900

TTCAAAGTCG ACTATGGGAG CTTCGCCTTT GTTAGAGATA GAGCTGGTTT TAATGGTACT 960

TCAGTAGAAG GGCAGTGCAA AGCCACTCCT GGAACAAAGA TTGTAGGTTA CTCAACACAT 1020

CATGAGCATC TCCAACGCCA GAGGGTCTCA TTTGAGCAGG TAAAACGGAT AATGGAGCTG 1080

CTAGAGTACA TAGAAGCACT TTATCCATCA TTGCAGGCTC TTCAGAAGGA CTATGAAAAA 1140

TATGCTGCAA AAGACTTCCA GGACAGGGTG CAGGCACTCT GTTTGTGGTT AAACATCACA 1200

AAAGACTTAA ATCAGAAATT AAGGATTATG GGCACTGTTT TGGGCATCAA GAATTTATCA 1260

GACATTGGCT GGCCAGTGTT TGAAATCCCT TCCCCTCGAC CATCCAAAGG TAATGAGCCG 1320

GAGTATGAGG GTGATGACAC AGAAGGAGAA TTAAAGGAGT TGGAAAGTAG TACGGATGAG 1380

AGTGAAGAAG AACAAATCTC TGATCCTAGG GTACCGGAAA TCAGACAGCC CATAGATAAC 1440

AGCTTCGACA TCCAGTCGCG GGACTGCATA TCCAAGAAGC TTGAGAGGCT CGAATCTGAG 1500

GATGATTCTC TTGGCTGGGG AGCACCAGAC TGGAGCACAG AAGCAGGCTT TAGTAGACAT 1560

TGTCTGACTT CTATTTATAG ACCATTTGTA GACAAAGCAC TGAAGCAGAT GGGGTTAAGA 1620

AAGTTAATTT TAAGACTTCA CAAGCTAATG GATGGTTCCT TGCAAAGGGC ACGTATAGCA 1680

TTGGTAAAGA ACGATCGTCC AGTGGAGTTT TCTGAATTTC CAGATCCCAT GTGGGGTTCA 1740

GATTATGTGC AGTTGTCAAG GACACCACCT TCATCTGAGG AGAAATGCAG TGCTGTGTCG 1800

TGGGAGGAGC TGAAGGCCAT GGATTTACCT TCATTCGAAC CTGCCTTCCT AGTTCTCTGC 1860

CGAGTCCTTC TGAATGTCAT ACATGAGTGT CTGAAGTTAA GATTGGAGCA GAGACCTGCT 1920

GGAGAACCAT CTCTCTTGAG TATTAAGCAG CTGGTGAGAG AGTGTAAGGA GGTCCTGAAG 1980

GGCGGCCTGC TGATGAAGCA GTACTACCAG TTCATGCTGC AGGAGGTTCT GGAGGACTTG 2040

GAGAAGCCCG ACTGCAACAT TGACGCTTTT GAAGAGGATC TACATAAAAT GCTTATGGTG 2100

TATTTTGATT ACATGAGAAG CTGGATCCAA ATGCTACAGC AATTACCTCA AGCATCGCAT 2160

AGTTTAAAAA ATCTGTTAGA AGAAGAATGG AATTTCACCA AAGAAATAAC TCATTACATA 2220

CGGGGAGGAG AAGCACAGGC CGGGAAGCTT TTCTGTGACA TTGCAGGAAT GCTGCTGAAA 2280

TCTACAGGAA GTTTTTTAGA ATTTGGCTTA CAGGAGAGCT GTGCTGAATT TTGGACTAGT 2340

GCGGATGACA GCAGTGCTTC CGACGAAATC AGGAGGTCTG TTATAGAGAT CAGTCGAGCC 2400

CTGAAGGAGC TCTTCCATGA AGCCAGAGAA AGGGCTTCCA AAGCACTTGG ATTTGCTAAA 2460

ATGTTGAGAA AGGACCTGGA AATAGCAGCA GAATTCAGGC TTTCAGCCCC AGTTAGAGAC 2520

CTCCTGGATG TTCTGAAATC AAAACAGTAT GTCAAGGTGC AAATTCCTGG GTTAGAAAAC 2580

TTGCAAATGT TTGTTCCAGA CACTCTTGCT GAGGAGAAGA GTATTATTTT GCAGTTACTC 2640

AATGCAGCTG CAGGAAAGGA CTGTTCAAAA GATTCAGATG ACGTACTCAT CGATGCCTAT 2700

CTGCTTCTGA CCAAGCACGG TGATCGAGCC CGTGATTCAG AGGACAGCTG GGGCACCTGG 2760

GAGGCACAGC CTGTCAAAGT CGTGCCTCAG GTGGAGACTG TTGACACCCT GAGAAGCATG 2820

CAGGTGGATA ATCTTTTACT AGTTGTCATG CAGTCTGCGC ATCTCACAAT TCAGAGAAAA 2880

GCTTTCCAGC AGTCCATTGA GGGACTTATG ACTCTGTGCC AGGAGCAGAC ATCCAGTCAG 2940

CCGGTCATCG CCAAAGCTTT GCAGCAGCTG AAGAATGATG CATTGGAGCT ATGCAACAGG 3000

ATAAGCAATG CCATTGACCG CGTGGACCAC ATGTTCACAT CAGAATTTGA TGCTGAGGTT 3060

GATGAATCTG AATCTGTCAC CTTGCAACAG TACTACCGAG AAGCAATGAT TCAGGGGTAC 3120

AATTTTGGAT TTGAGTATCA TAAAGAAGTT GTTCGTTTGA TGTCTGGGGA GTTTAGACAG 3180

AAGATAGGAG ACAAATATAT AAGCTTTGCC CGGAAGTGGA TGAATTATGT CCTGACTAAA 3240

TGTGAGAGTG GTAGAGGTAC AAGACCCAGG TGGGCGACTC AAGGATTTGA TTTTCTACAA 3300

GCAATTGAAC CTGCCTTTAT TTCAGCTTTA CCAGAAGATG ACTTCTTGAG TTTACAAGCC 3360

TTGATGAATG AATGCATTGG CCATGTCATA GGAAAACCAC ACAGTCCTGT TACAGGTTTG 3420

TACCTTGCCA TTCATCGGAA CAGCCCCCGT CCTATGAAGG TACCTCGATG CCATAGTGAC 3480

CCTCCTAACC CACACCTCAT TATCCCCACT CCAGAGGGAT TCAGCACTCG GAGCATGCCT 3540

TCCGACGCGC GGAGCCATGG CAGCCCTGCT GCTGCTGCTG CTGCTGCTGC TGCTGCTGTT 3600

GCTGCCAGTC GGCCCAGCCC CTCTGGTGGT GACTCTGTGC TGCCCAAATC CATCAGCAGT 3660

GCCCATGATA CCAGGGGTTC CAGCGTTCCT GAAAATGATC GATTGGCTTC CATAGCTGCT 3720

GAATTGCAGT TTAGGTCCCT GAGTCGTCAC TCAAGCCCCA CGGAGGAGCG AGATGAACCA 3780

GCATATCCAA GAGGAGATTC AAGTGGGTCC ACAAGAAGAA GTTGGGAACT TCGGACACTA 3840

ATCAGCCAGA GTAAAGATAC TGCTTCTAAA CTAGGACCCA TAGAAGCTAT CCAGAAGTCA 3900

GTCCGATTGT TTGAAGAAAA GAGGTACCGA GAAATGAGGA GAAAGAATAT CATTGGTCAA 3960

GTTTGTGATA CGCCTAAGTC CTATGATAAT GTTATGCACG TTGGCTTGAG GAAGGTGACC 4020

TTCAAATGGC AAAGAGGAAA CAAAATTGGA GAAGGCCAGT ATGGGAAGGT GTACACCTGC 4080

ATCAGCGTCG ACACCGGGGA GCTGATGGCC ATGAAAGAGA TTCGATTTCA ACCTAATGAC 4140

CATAAGACTA TCAAGGAAAC TGCAGACGAA TTGAAAATAT TCGAAGGCAT CAAACACCCC 4200

AATCTGGTTC GGTATTTTGG TGTGGAGCTC CATAGAGAAG AAATGTACAT CTTCATGGAG 4260

TACTGCGATG AGGGGACTTT AGAAGAGGTG TCAAGGCTGG GACTTCAGGA ACATGTGATT 4320

AGGCTGTATT CAAAGCAGAT CACCATTGCG ATCAACGTCC TCCATGAGCA TGGCATAGTC 4380

CACCGTGACA TTAAAGGTGC CAATATCTTC CTTACCTCAT CTGGATTAAT CAAACTGGGA 4440

GATTTTGGAT GTTCAGTAAA GCTCAAAAAC AATGCCCAGA CCATGCCTGG TGAAGTGAAC 4500

AGCACCCTGG GGACAGCAGC ATACATGGCA CCTGAAGTCA TCACTCGTGC CAAAGGAGAG 4560

GGCCATGGGC GTGCGGCCGA CATCTGGAGT CTGGGGTGTG TTGTCATAGA GATGGTGACT 4620

GGCAAGAGGC CTTGGCATGA GTATGAGCAC AACTTTCAAA TTATGTATAA AGTGGGGATG 4680

GGACATAAGC CACCAATCCC TGAAAGATTA AGCCCTGAAG GAAAGGACTT CCTTTCTCAC 4740

TGCCTTGAGA GTGACCCAAA GATGAGATGG ACCGCCAGCC AGCTCCTCGA CCATTCGTTT 4800

GTCAAGGTTT GCACAGATGA AGAATGA 4827

Domain Profile

S: 1     wqkrevigkGsfgsvyaaldvdtgellavKeveikpekksekkevkalkeEikllkelkH  60

         wq+++ ig+G++g+vy++++vdtgel+a+Ke++++p++++   ++k+ ++E+k+++ +kH

Q: 1343  WQRGNKIGEGQYGKVYTCISVDTGELMAMKEIRFQPNDHK---TIKETADELKIFEGIKH  1399

         99********************************999876...799**************

S: 61    enivkyyGveveeeelkiflEyvegGslssllekygkleesvikkytkqileglayLHek  120

         +n+v+y+Gve ++ee++if+Ey+++G+l+++ +    l+e+vi+ y+kqi  ++++LHe+

Q: 1400  PNLVRYFGVELHREEMYIFMEYCDEGTLEEVSRL--GLQEHVIRLYSKQITIAINVLHEH  1457

         *******************************884..7***********************

S: 121   kivHRDiKgaNilldssgvvklsDFGaskrlaelntka.aelesltGtlaymaPEvikk.  178

         +ivHRDiKgaNi+l+ssg++kl+DFG+s +l+++++++ +e++s+ Gt+aymaPEvi++

Q: 1458  GIVHRDIKGANIFLTSSGLIKLGDFGCSVKLKNNAQTMpGEVNSTLGTAAYMAPEVITRa  1517

         *******************************9988776256*****************98

S: 179   kekgygrkaDiWslgcvviemltgkrPfaeldeelaimfkvgakktkPeipeklseegkd  238

         k +g+gr+aDiWslgcvviem+tgkrP++e++++++im+kvg+ + kP+ipe+ls+egkd

Q: 1518  KGEGHGRAADIWSLGCVVIEMVTGKRPWHEYEHNFQIMYKVGMGH-KPPIPERLSPEGKD  1576

         899******************************************.**************

S: 239   flekcleldpkkrpsaeeLLkhefl  263

         fl++cle+dpk r++a++LL+h+f+

Q: 1577  FLSHCLESDPKMRWTASQLLDHSFV  1601

         ***********************96

Domain Sequence
(FASTA)

WQRGNKIGEG QYGKVYTCIS VDTGELMAMK EIRFQPNDHK TIKETADELK IFEGIKHPNL 60

VRYFGVELHR EEMYIFMEYC DEGTLEEVSR LGLQEHVIRL YSKQITIAIN VLHEHGIVHR 120

DIKGANIFLT SSGLIKLGDF GCSVKLKNNA QTMPGEVNST LGTAAYMAPE VITRAKGEGH 180

GRAADIWSLG CVVIEMVTGK RPWHEYEHNF QIMYKVGMGH KPPIPERLSP EGKDFLSHCL 240

ESDPKMRWTA SQLLDHSFV 259

Keyword

Alternative splicing; ATP-binding; Complete proteome; Cytoplasm; Kinase; Magnesium; Metal-binding; Nucleotide-binding; Phosphoprotein; Polymorphism; Reference proteome; Serine/threonine-protein kinase; Transferase.

Sequence Source

Ensembl

Orthology

Ortholog group

Ailuropoda melanoleuca"; ?>Anolis carolinensis"; ?>Bos taurus"; ?>Caenorhabditis elegans"; ?>Callithrix jacchus"; ?>Canis familiaris"; ?>Cavia porcellus"; ?>Ciona intestinalis"; ?>Ciona savignyi"; ?>Danio rerio"; ?>Drosophila melanogaster"; ?>Equus caballus"; ?>Felis catus"; ?>Gallus gallus"; ?>Gasterosteus aculeatus"; ?>Gorilla gorilla"; ?>Ictidomys tridecemlineatus"; ?>Latimeria chalumnae"; ?>Loxodonta africana"; ?>Macaca mulatta"; ?>Meleagris gallopavo"; ?>Monodelphis domestica"; ?>Mus musculus"; ?>Mustela putorius furo"; ?>Myotis lucifugus"; ?>Oreochromis niloticus"; ?>Ornithorhynchus anatinus"; ?>Oryctolagus cuniculus"; ?>Oryzias latipes"; ?>Otolemur garnettii"; ?>Pelodiscus sinensis"; ?>Petromyzon marinus"; ?>Pongo abelii"; ?>Rattus norvegicus"; ?>Sarcophilus harrisii"; ?>Sus scrofa"; ?>Taeniopygia guttata"; ?>Takifugu rubripes"; ?>Tetraodon nigroviridis"; ?>Xiphophorus maculatus"; ?>Saccharomyces cerevisiae"; ?>Schizosaccharomyces pombe"; ?>

Gene Ontology

GO:0005737	; C:cytoplasm
GO:0048471	; C:perinuclear region of cytoplasm
GO:0005524	; F:ATP binding
GO:0004709	; F:MAP kinase kinase kinase activity
GO:0046872	; F:metal ion binding
GO:0007254	; P:JNK cascade
GO:0019100	; P:male germ-line sex determination
GO:0043507	; P:positive regulation of JUN kinase activity

KEGG

hsa:4216

;

InterPros

IPR011009	; Kinase-like_dom.
IPR000719	; Prot_kinase_cat_dom.
IPR017441	; Protein_kinase_ATP_BS.
IPR002290	; Ser/Thr_dual-sp_kinase_dom.
IPR008271	; Ser/Thr_kinase_AS.

Pfam

PF00069

; Pkinase; 1.

SMARTs

SM00220

; S_TKc; 1.

Prosites

PS00107	; PROTEIN_KINASE_ATP; 1.
PS50011	; PROTEIN_KINASE_DOM; 1.
PS00108	; PROTEIN_KINASE_ST; 1.

Prints

Created Date

20-Feb-2013