EKS-HOS-00400

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EKS-HOS-00400

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
STE/STE11	325.0	2.2E-96	1243	1507	265

Status

Reviewed

Ensembl Protein

ENSP00000382423

UniProt Accession

Q13233;

Protein Name

Mitogen-activated protein kinase kinase kinase 1

Protein Synonyms/Alias

MAPK/ERK kinase kinase 1; MEK kinase 1; MEKK 1;

Gene Name

MAP3K1

Gene Synonyms/Alias

MAP3K1; MAPKKK1, MEKK, MEKK1;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
ENSG00000095015	ENSP00000382423	ENST00000399503

Organism

Homo sapiens

Functional Description

Component of a protein kinase signal transductioncascade. Activates the ERK and JNK kinase pathways by phosphorylation of MAP2K1 and MAP2K4. Activates CHUK and IKBKB, the central protein kinases of the NF-kappa-B pathway.

Protein Length

1512

Protein Sequence
(FASTA)

MAAAAGNRAS SSGFPGARAT SPEAGGGGGA LKASSAPAAA AGLLREAGSG GRERADWRRR 60

QLRKVRSVEL DQLPEQPLFL AASPPASSTS PSPEPADAAG SGTGFQPVAV PPPHGAASRG 120

GAHLTESVAA PDSGASSPAA AEPGEKRAPA AEPSPAAAPA GREMENKETL KGLHKMDDRP 180

EERMIREKLK ATCMPAWKHE WLERRNRRGP VVVKPIPVKG DGSEMNHLAA ESPGEVQASA 240

ASPASKGRRS PSPGNSPSGR TVKSESPGVR RKRVSPVPFQ SGRITPPRRA PSPDGFSPYS 300

PEETNRRVNK VMRARLYLLQ QIGPNSFLIG GDSPDNKYRV FIGPQNCSCA RGTFCIHLLF 360

VMLRVFQLEP SDPMLWRKTL KNFEVESLFQ KYHSRRSSRI KAPSRNTIQK FVSRMSNSHT 420

LSSSSTSTSS SENSIKDEEE QMCPICLLGM LDEESLTVCE DGCRNKLHHH CMSIWAEECR 480

RNREPLICPL CRSKWRSHDF YSHELSSPVD SPSSLRAAQQ QTVQQQPLAG SRRNQESNFN 540

LTHYGTQQIP PAYKDLAEPW IQVFGMELVG CLFSRNWNVR EMALRRLSHD VSGALLLANG 600

ESTGNSGGSS GSSPSGGATS GSSQTSISGD VVEACCSVLS MVCADPVYKV YVAALKTLRA 660

MLVYTPCHSL AERIKLQRLL QPVVDTILVK CADANSRTSQ LSISTLLELC KGQAGELAVG 720

REILKAGSIG IGGVDYVLNC ILGNQTESNN WQELLGRLCL IDRLLLEFPA EFYPHIVSTD 780

VSQAEPVEIR YKKLLSLLTF ALQSIDNSHS MVGKLSRRIY LSSARMVTTV PHVFSKLLEM 840

LSVSSSTHFT RMRRRLMAIA DEVEIAEAIQ LGVEDTLDGQ QDSFLQASVP NNYLETTENS 900

SPECTVHLEK TGKGLCATKL SASSEDISER LASISVGPSS STTTTTTTTE QPKPMVQTKG 960

RPHSQCLNSS PLSHHSQLMF PALSTPSSST PSVPAGTATD VSKHRLQGFI PCRIPSASPQ 1020

TQRKFSLQFH RNCPENKDSD KLSPVFTQSR PLPSSNIHRP KPSRPTPGNT SKQGDPSKNS 1080

MTLDLNSSSK CDDSFGCSSN SSNAVIPSDE TVFTPVEEKC RLDVNTELNS SIEDLLEASM 1140

PSSDTTVTFK SEVAVLSPEK AENDDTYKDD VNHNQKCKEK MEAEEEEALA IAMAMSASQD 1200

ALPIVPQLQV ENGEDIIIIQ QDTPETLPGH TKAKQPYRED TEWLKGQQIG LGAFSSCYQA 1260

QDVGTGTLMA VKQVTYVRNT SSEQEEVVEA LREEIRMMSH LNHPNIIRML GATCEKSNYN 1320

LFIEWMAGGS VAHLLSKYGA FKESVVINYT EQLLRGLSYL HENQIIHRDV KGANLLIDST 1380

GQRLRIADFG AAARLASKGT GAGEFQGQLL GTIAFMAPEV LRGQQYGRSC DVWSVGCAII 1440

EMACAKPPWN AEKHSNHLAL IFKIASATTA PSIPSHLSPG LRDVALRCLE LQPQDRPPSR 1500

ELLKHPVFRT TW 1512

Nucleotide Sequence
(FASTA)

ATGGCGGCGG CGGCGGGGAA TCGCGCCTCG TCGTCGGGAT TCCCGGGCGC CAGGGCTACG 60

AGCCCTGAGG CAGGCGGCGG CGGAGGAGCC CTCAAGGCGA GCAGCGCGCC CGCGGCTGCC 120

GCGGGACTGC TGCGGGAGGC GGGCAGCGGG GGCCGCGAGC GGGCGGACTG GCGGCGGCGG 180

CAGCTGCGCA AAGTGCGGAG TGTGGAGCTG GACCAGCTGC CTGAGCAGCC GCTCTTCCTT 240

GCCGCCTCAC CGCCGGCCTC CTCGACTTCC CCGTCGCCGG AGCCCGCGGA CGCAGCGGGG 300

AGTGGGACCG GCTTCCAGCC TGTGGCGGTG CCGCCGCCCC ACGGAGCCGC GAGCCGCGGC 360

GGCGCCCACC TTACCGAGTC GGTGGCGGCG CCGGACAGCG GCGCCTCGAG TCCCGCAGCG 420

GCCGAGCCCG GGGAGAAGCG GGCGCCCGCC GCCGAGCCGT CTCCTGCAGC GGCCCCCGCC 480

GGTCGTGAGA TGGAGAATAA AGAAACTCTC AAAGGGTTGC ACAAGATGGA TGATCGTCCA 540

GAGGAACGAA TGATCAGGGA GAAACTGAAG GCAACCTGTA TGCCAGCCTG GAAGCACGAA 600

TGGTTGGAAA GGAGAAATAG GCGAGGGCCT GTGGTGGTAA AACCAATCCC AGTTAAAGGA 660

GATGGATCTG AAATGAATCA CTTAGCAGCT GAGTCTCCAG GAGAGGTCCA GGCAAGTGCG 720

GCTTCACCAG CTTCCAAAGG CCGACGCAGT CCTTCTCCTG GCAACTCCCC ATCAGGTCGC 780

ACAGTGAAAT CAGAATCTCC AGGAGTAAGG AGAAAAAGAG TTTCCCCAGT GCCTTTTCAG 840

AGTGGCAGAA TCACACCACC CCGAAGAGCC CCTTCACCAG ATGGCTTCTC ACCATATAGC 900

CCTGAGGAAA CAAACCGCCG TGTTAACAAA GTGATGCGGG CCAGACTGTA CTTACTGCAG 960

CAGATAGGGC CTAACTCTTT CCTGATTGGA GGAGACAGCC CAGACAATAA ATACCGGGTG 1020

TTTATTGGGC CTCAGAACTG CAGCTGTGCA CGTGGAACAT TCTGTATTCA TCTGCTATTT 1080

GTGATGCTCC GGGTGTTTCA ACTAGAACCT TCAGACCCAA TGTTATGGAG AAAAACTTTA 1140

AAGAATTTTG AGGTTGAGAG TTTGTTCCAG AAATATCACA GTAGGCGTAG CTCAAGGATC 1200

AAAGCTCCAT CTCGTAACAC CATCCAGAAG TTTGTTTCAC GCATGTCAAA TTCTCATACA 1260

TTGTCATCAT CTAGTACTTC TACGTCTAGT TCAGAAAACA GCATAAAGGA TGAAGAGGAA 1320

CAGATGTGTC CTATTTGCTT GTTGGGCATG CTTGATGAAG AAAGTCTTAC AGTGTGTGAA 1380

GACGGCTGCA GGAACAAGCT GCACCACCAC TGCATGTCAA TTTGGGCAGA AGAGTGTAGA 1440

AGAAATAGAG AACCTTTAAT ATGTCCCCTT TGTAGATCTA AGTGGAGATC TCATGATTTC 1500

TACAGCCACG AGTTGTCAAG TCCTGTGGAT TCCCCTTCTT CCCTCAGAGC TGCACAGCAG 1560

CAAACCGTAC AGCAGCAGCC TTTGGCTGGA TCACGAAGGA ATCAAGAGAG CAATTTTAAC 1620

CTTACTCATT ATGGAACTCA GCAAATCCCT CCTGCTTACA AAGATTTAGC TGAGCCATGG 1680

ATTCAGGTGT TTGGAATGGA ACTCGTTGGC TGCTTATTTT CTAGAAACTG GAATGTGAGA 1740

GAGATGGCCC TCAGGCGTCT TTCCCATGAT GTCAGTGGGG CCCTGCTGTT GGCAAATGGG 1800

GAGAGCACTG GAAATTCTGG GGGCAGCAGT GGAAGCAGCC CGAGTGGGGG AGCCACCAGT 1860

GGGTCTTCCC AGACCAGTAT CTCAGGAGAT GTGGTGGAGG CATGCTGCAG CGTTCTGTCA 1920

ATGGTCTGTG CTGACCCTGT CTACAAAGTG TACGTTGCTG CTTTAAAAAC ATTGAGAGCC 1980

ATGCTGGTAT ATACTCCTTG CCACAGTTTA GCGGAAAGAA TCAAACTTCA GAGACTTCTC 2040

CAGCCAGTTG TAGACACCAT CCTAGTCAAA TGTGCAGATG CCAATAGCCG CACAAGTCAG 2100

CTGTCCATAT CAACACTGTT GGAACTGTGC AAAGGCCAAG CAGGAGAGTT GGCAGTTGGC 2160

AGAGAAATAC TAAAAGCTGG ATCCATTGGT ATTGGTGGTG TTGATTATGT CTTAAATTGT 2220

ATTCTTGGAA ACCAAACTGA ATCAAACAAT TGGCAAGAAC TTCTTGGCCG CCTTTGTCTT 2280

ATAGATAGAC TGTTGTTGGA ATTTCCTGCT GAATTTTATC CTCATATTGT CAGTACTGAT 2340

GTTTCACAAG CTGAGCCTGT TGAAATCAGG TATAAGAAGC TGCTGTCCCT CTTAACCTTT 2400

GCTTTGCAGT CCATTGATAA TTCCCACTCA ATGGTTGGCA AACTTTCCAG AAGGATCTAC 2460

TTGAGTTCTG CAAGAATGGT TACTACAGTA CCCCATGTGT TTTCAAAACT GTTAGAAATG 2520

CTGAGTGTTT CCAGTTCCAC TCACTTCACC AGGATGCGTC GCCGTTTGAT GGCTATTGCA 2580

GATGAGGTGG AAATTGCCGA AGCCATCCAG TTGGGCGTAG AAGACACTTT GGATGGTCAA 2640

CAGGACAGCT TCTTGCAGGC ATCTGTTCCC AACAACTATC TGGAAACCAC AGAGAACAGT 2700

TCCCCTGAGT GCACAGTCCA TTTAGAGAAA ACTGGAAAAG GATTATGTGC TACAAAATTG 2760

AGTGCCAGTT CAGAGGACAT TTCTGAGAGA CTGGCCAGCA TTTCAGTAGG ACCTTCTAGT 2820

TCAACAACAA CAACAACAAC AACAACAGAG CAACCAAAGC CAATGGTTCA AACAAAAGGC 2880

AGACCCCACA GTCAGTGTTT GAACTCCTCT CCTTTATCTC ATCATTCCCA ATTAATGTTT 2940

CCAGCCTTGT CAACCCCTTC TTCTTCTACC CCATCTGTAC CAGCTGGCAC TGCAACAGAT 3000

GTCTCTAAGC ATAGACTTCA GGGATTCATT CCCTGCAGAA TACCTTCTGC ATCTCCTCAA 3060

ACACAGCGCA AGTTTTCTCT ACAATTCCAC AGAAACTGTC CTGAAAACAA AGACTCAGAT 3120

AAACTTTCCC CAGTCTTTAC TCAGTCAAGA CCCTTGCCCT CCAGTAACAT ACACAGGCCA 3180

AAGCCATCTA GACCTACCCC AGGTAATACA AGTAAACAGG GAGATCCCTC AAAAAATAGC 3240

ATGACACTTG ATCTGAACAG TAGTTCCAAA TGTGATGACA GCTTTGGCTG TAGCAGCAAT 3300

AGTAGTAATG CTGTTATACC CAGTGACGAG ACAGTGTTCA CCCCAGTAGA GGAGAAATGC 3360

AGATTAGATG TCAATACAGA GCTCAACTCC AGTATTGAGG ACCTTCTTGA AGCATCTATG 3420

CCTTCAAGTG ATACAACAGT AACTTTTAAG TCAGAAGTTG CTGTCCTGTC TCCTGAAAAG 3480

GCTGAAAATG ATGATACCTA CAAAGATGAT GTGAATCATA ATCAAAAGTG CAAAGAGAAG 3540

ATGGAAGCTG AAGAAGAAGA AGCTTTAGCA ATTGCCATGG CAATGTCAGC GTCTCAGGAT 3600

GCCCTCCCCA TAGTTCCTCA GCTGCAGGTT GAAAATGGAG AAGATATCAT CATTATTCAA 3660

CAGGATACAC CAGAGACTCT ACCAGGACAT ACCAAAGCAA AACAACCGTA TAGAGAAGAC 3720

ACTGAATGGC TGAAAGGTCA ACAGATAGGC CTTGGAGCAT TTTCTTCTTG TTATCAGGCT 3780

CAAGATGTGG GAACTGGAAC TTTAATGGCT GTTAAACAGG TGACTTATGT CAGAAACACA 3840

TCTTCTGAGC AAGAAGAAGT AGTAGAAGCA CTAAGAGAAG AGATAAGAAT GATGAGCCAT 3900

CTGAATCATC CAAACATCAT TAGGATGTTG GGAGCCACGT GTGAGAAGAG CAATTACAAT 3960

CTCTTCATTG AATGGATGGC AGGGGGATCG GTGGCTCATT TGCTGAGTAA ATATGGAGCC 4020

TTCAAAGAAT CAGTAGTTAT TAACTACACT GAACAGTTAC TCCGTGGCCT TTCGTATCTC 4080

CATGAAAACC AAATCATTCA CAGAGATGTC AAAGGTGCCA ATTTGCTAAT TGACAGCACT 4140

GGTCAGAGAC TAAGAATTGC AGATTTTGGA GCTGCAGCCA GGTTGGCATC AAAAGGAACT 4200

GGTGCAGGAG AGTTTCAGGG ACAATTACTG GGGACAATTG CATTTATGGC ACCTGAGGTA 4260

CTAAGAGGTC AACAGTATGG AAGGAGCTGT GATGTATGGA GTGTTGGCTG TGCTATTATA 4320

GAAATGGCTT GTGCAAAACC ACCATGGAAT GCAGAAAAAC ACTCCAATCA TCTTGCTTTG 4380

ATATTTAAGA TTGCTAGTGC AACTACTGCT CCATCGATCC CTTCACATTT GTCTCCTGGT 4440

TTACGAGATG TGGCTCTTCG TTGTTTAGAA CTTCAACCTC AGGACAGACC TCCATCAAGA 4500

GAGCTACTGA AGCATCCAGT CTTTCGTACT ACATGGTAG 4539

Domain Profile

S: 1     wqkrevigkGsfgsvyaaldvdtgellavKeveikpekksekk.evkalkeEikllkelk  59

         w k+++ig G+f+s+y+a+dv tg+l+avK+v++ ++++se++ +v+al+eEi+++++l+

Q: 1243  WLKGQQIGLGAFSSCYQAQDVGTGTLMAVKQVTYVRNTSSEQEeVVEALREEIRMMSHLN  1302

         899*******************************999998887699**************

S: 60    HenivkyyGveveeeelkiflEyvegGslssllekygkleesvikkytkqileglayLHe  119

         H+ni++++G+++e++++++f+E+++gGs+++ll+kyg+++esv+ +yt+q+l+gl+yLHe

Q: 1303  HPNIIRMLGATCEKSNYNLFIEWMAGGSVAHLLSKYGAFKESVVINYTEQLLRGLSYLHE  1362

         ************************************************************

S: 120   kkivHRDiKgaNillds.sgvvklsDFGaskrlaelntkaae.lesltGtlaymaPEvik  177

         ++i+HRD+KgaN+l+ds  + ++++DFGa++rla++ t a+e + +l Gt+a+maPEv++

Q: 1363  NQIIHRDVKGANLLIDStGQRLRIADFGAAARLASKGTGAGEfQGQLLGTIAFMAPEVLR  1422

         *****************7789******************9996779*************9

S: 178   kkekgygrkaDiWslgcvviemltgkrPfa..eldeelaimfkvgakktkPeipeklsee  235

         ++  +ygr++D+Ws+gc++iem+++k+P++  +++++la++fk+++++t+P+ip++ls+

Q: 1423  GQ--QYGRSCDVWSVGCAIIEMACAKPPWNaeKHSNHLALIFKIASATTAPSIPSHLSPG  1480

         98..8************************87678899***********************

S: 236   gkdflekcleldpkkrpsaeeLLkhef  262

          +d+  +clel+p++rp ++eLLkh++

Q: 1481  LRDVALRCLELQPQDRPPSRELLKHPV  1507

         *************************86

Domain Sequence
(FASTA)

WLKGQQIGLG AFSSCYQAQD VGTGTLMAVK QVTYVRNTSS EQEEVVEALR EEIRMMSHLN 60

HPNIIRMLGA TCEKSNYNLF IEWMAGGSVA HLLSKYGAFK ESVVINYTEQ LLRGLSYLHE 120

NQIIHRDVKG ANLLIDSTGQ RLRIADFGAA ARLASKGTGA GEFQGQLLGT IAFMAPEVLR 180

GQQYGRSCDV WSVGCAIIEM ACAKPPWNAE KHSNHLALIF KIASATTAPS IPSHLSPGLR 240

DVALRCLELQ PQDRPPSREL LKHPV 265

Keyword

Acetylation; ATP-binding; Complete proteome; Kinase; Magnesium; Metal-binding; Nucleotide-binding; Phosphoprotein; Polymorphism; Reference proteome; Serine/threonine-protein kinase; Transferase; Zinc; Zinc-finger.

Sequence Source

Ensembl

Orthology

Ortholog group

Ailuropoda melanoleuca"; ?>Anolis carolinensis"; ?>Bos taurus"; ?>Callithrix jacchus"; ?>Canis familiaris"; ?>Cavia porcellus"; ?>Ciona intestinalis"; ?>Ciona savignyi"; ?>Danio rerio"; ?>Dipodomys ordii"; ?>Equus caballus"; ?>Felis catus"; ?>Gallus gallus"; ?>Gorilla gorilla"; ?>Ictidomys tridecemlineatus"; ?>Latimeria chalumnae"; ?>Loxodonta africana"; ?>Macaca mulatta"; ?>Meleagris gallopavo"; ?>Monodelphis domestica"; ?>Mus musculus"; ?>Mustela putorius furo"; ?>Myotis lucifugus"; ?>Nomascus leucogenys"; ?>Ornithorhynchus anatinus"; ?>Oryctolagus cuniculus"; ?>Otolemur garnettii"; ?>Pan troglodytes"; ?>Pelodiscus sinensis"; ?>Petromyzon marinus"; ?>Pongo abelii"; ?>Rattus norvegicus"; ?>Sarcophilus harrisii"; ?>Sus scrofa"; ?>Taeniopygia guttata"; ?>Vicugna pacos"; ?>

Gene Ontology

GO:0005856	; C:cytoskeleton
GO:0005829	; C:cytosol
GO:0005524	; F:ATP binding
GO:0008545	; F:JUN kinase kinase activity
GO:0004706	; F:JUN kinase kinase kinase activity
GO:0004709	; F:MAP kinase kinase kinase activity
GO:0046625	; F:sphingolipid binding
GO:0004842	; F:ubiquitin-protein ligase activity
GO:0008270	; F:zinc ion binding
GO:0008637	; P:apoptotic mitochondrial changes
GO:0043010	; P:camera-type eye development
GO:0071260	; P:cellular response to mechanical stimulus
GO:0045087	; P:innate immune response
GO:0002755	; P:MyD88-dependent toll-like receptor signaling pathway
GO:0032232	; P:negative regulation of actin filament bundle assembly
GO:0018105	; P:peptidyl-serine phosphorylation
GO:0030838	; P:positive regulation of actin filament polymerization
GO:0043065	; P:positive regulation of apoptotic process
GO:0050434	; P:positive regulation of viral transcription
GO:0046777	; P:protein autophosphorylation
GO:0051259	; P:protein oligomerization
GO:0000209	; P:protein polyubiquitination
GO:0042787	; P:protein ubiquitination involved in ubiquitin-dependent protein catabolic process
GO:0030334	; P:regulation of cell migration
GO:0006970	; P:response to osmotic stress
GO:0008063	; P:Toll signaling pathway
GO:0034130	; P:toll-like receptor 1 signaling pathway
GO:0034134	; P:toll-like receptor 2 signaling pathway
GO:0034142	; P:toll-like receptor 4 signaling pathway
GO:0007179	; P:transforming growth factor beta receptor signaling pathway
GO:0042060	; P:wound healing

KEGG

hsa:4214

;

InterPros

IPR011009	; Kinase-like_dom.
IPR000719	; Prot_kinase_cat_dom.
IPR017441	; Protein_kinase_ATP_BS.
IPR002290	; Ser/Thr_dual-sp_kinase_dom.
IPR008271	; Ser/Thr_kinase_AS.
IPR001841	; Znf_RING.
IPR013083	; Znf_RING/FYVE/PHD.
IPR007527	; Znf_SWIM.

Pfam

PF00069

; Pkinase; 1.

SMARTs

SM00184	; RING; 1.
SM00220	; S_TKc; 1.

Prosites

PS00107	; PROTEIN_KINASE_ATP; 1.
PS50011	; PROTEIN_KINASE_DOM; 1.
PS00108	; PROTEIN_KINASE_ST; 1.
PS00518	; ZF_RING_1; FALSE_NEG.
PS50089	; ZF_RING_2; 1.
PS50966	; ZF_SWIM; 1.

Prints

Created Date

20-Feb-2013