EPS-HOS-00018

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EPS-HOS-00018

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
PPM/PP2C	293.7	7.3E-87	23	289	267

Status

Reviewed

Ensembl Protein

ENSP00000314850

UniProt Accession

P35813; B5BU11; O75551;

Protein Name

Protein phosphatase 1A

Protein Synonyms/Alias

Protein phosphatase 2C isoform alpha; PP2C-alpha; Protein phosphatase IA;

Gene Name

PPM1A

Gene Synonyms/Alias

PPM1A; PPPM1A;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
ENSG00000100614	ENSP00000432966	ENST00000529574
ENSG00000100614	ENSP00000436445	ENST00000532036
ENSG00000100614	ENSP00000431453	ENST00000528241
ENSG00000100614	ENSP00000437200	ENST00000531143
ENSG00000100614	ENSP00000327255	ENST00000325642
ENSG00000100614	ENSP00000435575	ENST00000531937
ENSG00000100614	ENSP00000314850	ENST00000325658
ENSG00000100614	ENSP00000435398	ENST00000525399
ENSG00000100614	ENSP00000378514	ENST00000395076

Organism

Homo sapiens

Functional Description

Enzyme with a broad specificity. Negatively regulatesTGF-beta signaling through dephosphorylating SMAD2 and SMAD3, resulting in their dissociation from SMAD4, nuclear export of the SMADs and termination of the TGF-beta-mediated signaling.

Protein Length

324

Protein Sequence
(FASTA)

MGAFLDKPKM EKHNAQGQGN GLRYGLSSMQ GWRVEMEDAH TAVIGLPSGL ESWSFFAVYD 60

GHAGSQVAKY CCEHLLDHIT NNQDFKGSAG APSVENVKNG IRTGFLEIDE HMRVMSEKKH 120

GADRSGSTAV GVLISPQHTY FINCGDSRGL LCRNRKVHFF TQDHKPSNPL EKERIQNAGG 180

SVMIQRVNGS LAVSRALGDF DYKCVHGKGP TEQLVSPEPE VHDIERSEED DQFIILACDG 240

IWDVMGNEEL CDFVRSRLEV TDDLEKVCNE VVDTCLYKGS RDNMSVILIC FPNAPKVSPE 300

AVKKEAELDK YLECRVEGGS FNKK 324

Nucleotide Sequence
(FASTA)

ATGGGAGCAT TTTTAGACAA GCCAAAGATG GAAAAGCATA ATGCCCAGGG GCAGGGTAAT 60

GGGTTGCGAT ATGGGCTAAG CAGCATGCAA GGCTGGCGTG TTGAAATGGA GGATGCACAT 120

ACGGCTGTGA TCGGTTTGCC AAGTGGACTT GAATCGTGGT CATTCTTTGC TGTGTATGAT 180

GGGCATGCTG GTTCTCAGGT TGCCAAATAC TGCTGTGAGC ATTTGTTAGA TCACATCACC 240

AATAACCAGG ATTTTAAAGG GTCTGCAGGA GCACCTTCTG TGGAAAATGT AAAGAATGGA 300

ATCAGAACAG GTTTTCTGGA GATTGATGAA CACATGAGAG TTATGTCAGA GAAGAAACAT 360

GGTGCAGATA GAAGTGGGTC AACAGCTGTA GGTGTCTTAA TTTCTCCCCA ACATACTTAT 420

TTCATTAACT GTGGAGACTC AAGAGGTTTA CTTTGTAGGA ACAGGAAAGT TCATTTCTTC 480

ACACAAGATC ACAAACCAAG TAATCCGCTG GAGAAAGAAC GAATTCAGAA TGCAGGTGGC 540

TCTGTAATGA TTCAGCGTGT GAATGGCTCT CTGGCTGTAT CGAGGGCCCT TGGGGATTTT 600

GATTACAAAT GTGTCCATGG AAAAGGTCCT ACTGAGCAGC TTGTCTCACC AGAGCCTGAA 660

GTCCATGATA TTGAAAGATC TGAAGAAGAT GATCAGTTCA TTATCCTTGC ATGTGATGGT 720

ATCTGGGATG TTATGGGAAA TGAAGAGCTC TGTGATTTTG TAAGATCCAG ACTTGAAGTC 780

ACTGATGACC TTGAGAAAGT TTGCAATGAA GTAGTCGACA CCTGTTTGTA TAAGGGAAGT 840

CGAGACAACA TGAGTGTGAT TTTGATCTGT TTTCCAAATG CACCCAAAGT ATCGCCAGAA 900

GCAGTGAAGA AGGAGGCAGA GTTGGACAAG TACCTGGAAT GCAGAGTAGA AGGTGGATCA 960

TTTAACAAAA AATAA 975

Domain Profile

S: 2     lvgvvsvngrrnemedavtsspnflkspekkdrlaffgvyDGhggsevakfcseklheil  61

         ++g++s++g+r emeda+t++ ++   p+  + ++ff+vyDGh+gs+vak+c e+l +++

Q: 23    RYGLSSMQGWRVEMEDAHTAVIGL---PSGLESWSFFAVYDGHAGSQVAKYCCEHLLDHI  79

         69****************984444...444445***************************

S: 62    aeele....sseseerkvkkalakaFlstdeelkvleeksldidksGstavvalikdskl  117

         +++ +    +++++ ++vk+ ++++Fl++de+++v++ek++  d+sGstav +li++++

Q: 80    TNNQDfkgsAGAPSVENVKNGIRTGFLEIDEHMRVMSEKKHGADRSGSTAVGVLISPQHT  139

         ***9999999999***********************************************

S: 118   yvanvGdSravLcrngkavelssdHkpsredEleRieeaggsvilkdgaRvlGvLavsRa  177

         y++n+GdSr +Lcrn+k++  ++dHkps++ E+eRi++aggsv+ +   Rv+G+LavsRa

Q: 140   YFINCGDSRGLLCRNRKVHFFTQDHKPSNPLEKERIQNAGGSVMIQ---RVNGSLAVSRA  196

         **********************************************...***********

S: 178   lGdlylk.........pyvtpePevtshrltsede.fliLasDGlwdvvsneeavslvrd  227

         lGd+++k         ++v+pePev+ +++++ed+ f+iLa+DG+wdv+ nee++++vr+

Q: 197   LGDFDYKcvhgkgpteQLVSPEPEVHDIERSEEDDqFIILACDGIWDVMGNEELCDFVRS  256

         ******************************999999************************

S: 228   vlekkeaeqeaakkLvklalargskdnvsaivv  260

         +le +++++++++++v+++l +gs+dn+s+i++

Q: 257   RLEVTDDLEKVCNEVVDTCLYKGSRDNMSVILI  289

         ****************************99976

Domain Sequence
(FASTA)

RYGLSSMQGW RVEMEDAHTA VIGLPSGLES WSFFAVYDGH AGSQVAKYCC EHLLDHITNN 60

QDFKGSAGAP SVENVKNGIR TGFLEIDEHM RVMSEKKHGA DRSGSTAVGV LISPQHTYFI 120

NCGDSRGLLC RNRKVHFFTQ DHKPSNPLEK ERIQNAGGSV MIQRVNGSLA VSRALGDFDY 180

KCVHGKGPTE QLVSPEPEVH DIERSEEDDQ FIILACDGIW DVMGNEELCD FVRSRLEVTD 240

DLEKVCNEVV DTCLYKGSRD NMSVILI 267

Keyword

3D-structure; Alternative splicing; Complete proteome; Hydrolase; Lipoprotein; Magnesium; Manganese; Metal-binding; Myristate; Nucleus; Phosphoprotein; Protein phosphatase; Reference proteome.

Sequence Source

Ensembl

Orthology

Ortholog group

Ailuropoda melanoleuca"; ?>Anolis carolinensis"; ?>Bos taurus"; ?>Callithrix jacchus"; ?>Canis familiaris"; ?>Cavia porcellus"; ?>Ciona intestinalis"; ?>Ciona savignyi"; ?>Danio rerio"; ?>Dipodomys ordii"; ?>Echinops telfairi"; ?>Equus caballus"; ?>Erinaceus europaeus"; ?>Felis catus"; ?>Gadus morhua"; ?>Gallus gallus"; ?>Gasterosteus aculeatus"; ?>Gorilla gorilla"; ?>Ictidomys tridecemlineatus"; ?>Latimeria chalumnae"; ?>Loxodonta africana"; ?>Macaca mulatta"; ?>Meleagris gallopavo"; ?>Monodelphis domestica"; ?>Mus musculus"; ?>Mustela putorius furo"; ?>Myotis lucifugus"; ?>Nomascus leucogenys"; ?>Oreochromis niloticus"; ?>Ornithorhynchus anatinus"; ?>Oryctolagus cuniculus"; ?>Oryzias latipes"; ?>Otolemur garnettii"; ?>Pan troglodytes"; ?>Pelodiscus sinensis"; ?>Pongo abelii"; ?>Pteropus vampyrus"; ?>Rattus norvegicus"; ?>Sarcophilus harrisii"; ?>Sorex araneus"; ?>Sus scrofa"; ?>Taeniopygia guttata"; ?>Takifugu rubripes"; ?>Tarsius syrichta"; ?>Tetraodon nigroviridis"; ?>Tursiops truncatus"; ?>Vicugna pacos"; ?>Xenopus tropicalis"; ?>Xiphophorus maculatus"; ?>Saccharomyces cerevisiae"; ?>Schizosaccharomyces pombe"; ?>

Gene Ontology

GO:0005829	; C:cytosol
GO:0043005	; C:neuron projection
GO:0005654	; C:nucleoplasm
GO:0005891	; C:voltage-gated calcium channel complex
GO:0033192	; F:calmodulin-dependent protein phosphatase activity
GO:0000287	; F:magnesium ion binding
GO:0030145	; F:manganese ion binding
GO:0004871	; F:signal transducer activity
GO:0007050	; P:cell cycle arrest
GO:0008286	; P:insulin receptor signaling pathway
GO:0010991	; P:negative regulation of SMAD protein complex assembly
GO:0000122	; P:negative regulation of transcription from RNA polymerase II promoter
GO:0030512	; P:negative regulation of transforming growth factor beta receptor signaling pathway
GO:0035970	; P:peptidyl-threonine dephosphorylation
GO:0043123	; P:positive regulation of I-kappaB kinase/NF-kappaB cascade
GO:0045893	; P:positive regulation of transcription, DNA-dependent
GO:0030177	; P:positive regulation of Wnt receptor signaling pathway
GO:0006367	; P:transcription initiation from RNA polymerase II promoter
GO:0007179	; P:transforming growth factor beta receptor signaling pathway
GO:0016055	; P:Wnt receptor signaling pathway

KEGG

hsa:5494

;

InterPros

IPR001932	; PP2C-like.
IPR012911	; PP2C_C.
IPR000222	; PP2C_Mn2_Asp60_BS.
IPR015655	; Protein_Pase_2C.

Pfam

PF00481	; PP2C; 1.
PF07830	; PP2C_C; 1.

SMARTs

SM00331	; PP2C_SIG; 1.
SM00332	; PP2Cc; 1.

Prosites

PS01032

; PP2C; 1.

Prints

Created Date

20-Feb-2013