EKS-HOS-00112

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EKS-HOS-00112

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
TK/Tec	481.8	2.0E-144	417	667	251

Status

Reviewed

Ensembl Protein

ENSP00000308774

UniProt Accession

P51813; A6NIH9; O60564; Q12871;

Protein Name

Cytoplasmic tyrosine-protein kinase BMX

Protein Synonyms/Alias

Bone marrow tyrosine kinase gene in chromosome X protein; Epithelial and endothelial tyrosine kinase; ETK; NTK38;

Gene Name

BMX

Gene Synonyms/Alias

BMX;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
ENSG00000102010	ENSP00000350224	ENST00000357607
ENSG00000102010	ENSP00000308774	ENST00000348343
ENSG00000102010	ENSP00000340082	ENST00000342014

Organism

Homo sapiens

Functional Description

Non-receptor tyrosine kinase that plays central butdiverse modulatory roles in various signaling processes involved in the regulation of actin reorganization, cell migration, cell proliferation and survival, cell adhesion, and apoptosis. Participates in signal transduction stimulated by growth factor receptors, cytokine receptors, G-protein coupled receptors, antigen receptors and integrins. Induces tyrosine phosphorylation of BCAR1 in response to integrin regulation. Activation of BMX by integrins is mediated by PTK2/FAK1, a key mediator of integrin signaling events leading to the regulation of actin cytoskeleton and cell motility. Plays a critical role in TNF-induced angiogenesis, and implicated in the signaling of TEK and FLT1 receptors, 2 important receptor families essential for angiogenesis. Required for the phosphorylation and activation of STAT3, a transcription factor involved in cell differentiation. Also involved in interleukin-6 (IL6) induced differentiation. Plays also a role in programming adaptive cytoprotection against extracellular stress in different cell systems, salivary epithelial cells, brain endothelial cells, and dermal fibroblasts. May be involved in regulation of endocytosis through its interaction with an endosomal protein RUFY1. May also play a role in the growth and differentiation of hematopoietic cells; as well as in signal transduction in endocardial and arterial endothelial cells.

Protein Length

675

Protein Sequence
(FASTA)

MDTKSILEEL LLKRSQQKKK MSPNNYKERL FVLTKTNLSY YEYDKMKRGS RKGSIEIKKI 60

RCVEKVNLEE QTPVERQYPF QIVYKDGLLY VYASNEESRS QWLKALQKEI RGNPHLLVKY 120

HSGFFVDGKF LCCQQSCKAA PGCTLWEAYA NLHTAVNEEK HRVPTFPDRV LKIPRAVPVL 180

KMDAPSSSTT LAQYDNESKK NYGSQPPSSS TSLAQYDSNS KKIYGSQPNF NMQYIPREDF 240

PDWWQVRKLK SSSSSEDVAS SNQKERNVNH TTSKISWEFP ESSSSEEEEN LDDYDWFAGN 300

ISRSQSEQLL RQKGKEGAFM VRNSSQVGMY TVSLFSKAVN DKKGTVKHYH VHTNAENKLY 360

LAENYCFDSI PKLIHYHQHN SAGMITRLRH PVSTKANKVP DSVSLGNGIW ELKREEITLL 420

KELGSGQFGV VQLGKWKGQY DVAVKMIKEG SMSEDEFFQE AQTMMKLSHP KLVKFYGVCS 480

KEYPIYIVTE YISNGCLLNY LRSHGKGLEP SQLLEMCYDV CEGMAFLESH QFIHRDLAAR 540

NCLVDRDLCV KVSDFGMTRY VLDDQYVSSV GTKFPVKWSA PEVFHYFKYS SKSDVWAFGI 600

LMWEVFSLGK QPYDLYDNSQ VVLKVSQGHR LYRPHLASDT IYQIMYSCWH ELPEKRPTFQ 660

QLLSSIEPLR EKDKH 675

Nucleotide Sequence
(FASTA)

ATGGATACAA AATCTATTCT AGAAGAACTT CTTCTCAAAA GATCACAGCA AAAGAAGAAA 60

ATGTCACCAA ATAATTACAA AGAACGGCTT TTTGTTTTGA CCAAAACAAA CCTTTCCTAC 120

TATGAATATG ACAAAATGAA AAGGGGCAGC AGAAAAGGAT CCATTGAAAT TAAGAAAATC 180

AGATGTGTGG AGAAAGTAAA TCTCGAGGAG CAGACGCCTG TAGAGAGACA GTACCCATTT 240

CAGATTGTCT ATAAAGATGG GCTTCTCTAT GTCTATGCAT CAAATGAAGA GAGCCGAAGT 300

CAGTGGTTGA AAGCATTACA AAAAGAGATA AGGGGTAACC CCCACCTGCT GGTCAAGTAC 360

CATAGTGGGT TCTTCGTGGA CGGGAAGTTC CTGTGTTGCC AGCAGAGCTG TAAAGCAGCC 420

CCAGGATGTA CCCTCTGGGA AGCATATGCT AATCTGCATA CTGCAGTCAA TGAAGAGAAA 480

CACAGAGTTC CCACCTTCCC AGACAGAGTG CTGAAGATAC CTCGGGCAGT TCCTGTTCTC 540

AAAATGGATG CACCATCTTC AAGTACCACT CTAGCCCAAT ATGACAACGA ATCAAAGAAA 600

AACTATGGCT CCCAGCCACC ATCTTCAAGT ACCAGTCTAG CGCAATATGA CAGCAACTCA 660

AAGAAAATCT ATGGCTCCCA GCCAAACTTC AACATGCAGT ATATTCCAAG GGAAGACTTC 720

CCTGACTGGT GGCAAGTAAG AAAACTGAAA AGTAGCAGCA GCAGTGAAGA TGTTGCAAGC 780

AGTAACCAAA AAGAAAGAAA TGTGAATCAC ACCACCTCAA AGATTTCATG GGAATTCCCT 840

GAGTCAAGTT CATCTGAAGA AGAGGAAAAC CTGGATGATT ATGACTGGTT TGCTGGTAAC 900

ATCTCCAGAT CACAATCTGA ACAGTTACTC AGACAAAAGG GAAAAGAAGG AGCATTTATG 960

GTTAGAAATT CGAGCCAAGT GGGAATGTAC ACAGTGTCCT TATTTAGTAA GGCTGTGAAT 1020

GATAAAAAAG GAACTGTCAA ACATTACCAC GTGCATACAA ATGCTGAGAA CAAATTATAC 1080

CTGGCAGAAA ACTACTGTTT TGATTCCATT CCAAAGCTTA TTCATTATCA TCAACACAAT 1140

TCAGCAGGCA TGATCACACG GCTCCGCCAC CCTGTGTCAA CAAAGGCCAA CAAGGTCCCC 1200

GACTCTGTGT CCCTGGGAAA TGGAATCTGG GAACTGAAAA GAGAAGAGAT TACCTTGTTG 1260

AAGGAGCTGG GAAGTGGCCA GTTTGGAGTG GTCCAGCTGG GCAAGTGGAA GGGGCAGTAT 1320

GATGTTGCTG TTAAGATGAT CAAGGAGGGC TCCATGTCAG AAGATGAATT CTTTCAGGAG 1380

GCCCAGACTA TGATGAAACT CAGCCATCCC AAGCTGGTTA AATTCTATGG AGTGTGTTCA 1440

AAGGAATACC CCATATACAT AGTGACTGAA TATATAAGCA ATGGCTGCTT GCTGAATTAC 1500

CTGAGGAGTC ACGGAAAAGG ACTTGAACCT TCCCAGCTCT TAGAAATGTG CTACGATGTC 1560

TGTGAAGGCA TGGCCTTCTT GGAGAGTCAC CAATTCATAC ACCGGGACTT GGCTGCTCGT 1620

AACTGCTTGG TGGACAGAGA TCTCTGTGTG AAAGTATCTG ACTTTGGAAT GACAAGGTAT 1680

GTTCTTGATG ACCAGTATGT CAGTTCAGTC GGAACAAAGT TTCCAGTCAA GTGGTCAGCT 1740

CCAGAGGTGT TTCATTACTT CAAATACAGC AGCAAGTCAG ACGTATGGGC ATTTGGGATC 1800

CTGATGTGGG AGGTGTTCAG CCTGGGGAAG CAGCCCTATG ACTTGTATGA CAACTCCCAG 1860

GTGGTTCTGA AGGTCTCCCA GGGCCACAGG CTTTACCGGC CCCACCTGGC ATCGGACACC 1920

ATCTACCAGA TCATGTACAG CTGCTGGCAC GAGCTTCCAG AAAAGCGTCC CACATTTCAG 1980

CAACTCCTGT CTTCCATTGA ACCACTTCGG GAAAAAGACA AGCATTGA 2028

Domain Profile

S: 1     ltllkelgsgqfgvvklgkwrakidvaikaikegamseedfieeakvmmklshpklvqly  60

         +tllkelgsgqfgvv+lgkw++++dva+k+ikeg+mse++f++ea++mmklshpklv++y

Q: 417   ITLLKELGSGQFGVVQLGKWKGQYDVAVKMIKEGSMSEDEFFQEAQTMMKLSHPKLVKFY  476

         699*********************************************************

S: 61    gvclkqkpiyivteylengcllnylrekkkklekelllemckdvcegmeylerksfihrd  120

         gvc+k++piyivtey++ngcllnylr++ k le ++llemc+dvcegm++le+++fihrd

Q: 477   GVCSKEYPIYIVTEYISNGCLLNYLRSHGKGLEPSQLLEMCYDVCEGMAFLESHQFIHRD  536

         ************************************************************

S: 121   laarnclvdeelvvkvsdfglaryvlddqyvssvgakfpvkwsppevlsyskyssksdvw  180

         laarnclvd++l+vkvsdfg++ryvlddqyvssvg+kfpvkws+pev++y+kyssksdvw

Q: 537   LAARNCLVDRDLCVKVSDFGMTRYVLDDQYVSSVGTKFPVKWSAPEVFHYFKYSSKSDVW  596

         ************************************************************

S: 181   afgvlmwevfsegklpyekksnsevvekvsrglrlyrpklaskkvyevmkscwkekpeer  240

         afg+lmwevfs+gk+py+ ++ns+vv kvs+g+rlyrp+las+++y++m+scw+e pe+r

Q: 597   AFGILMWEVFSLGKQPYDLYDNSQVVLKVSQGHRLYRPHLASDTIYQIMYSCWHELPEKR  656

         ************************************************************

S: 241   ptfkellsale  251

         ptf++lls++e

Q: 657   PTFQQLLSSIE  667

         ********986

Domain Sequence
(FASTA)

ITLLKELGSG QFGVVQLGKW KGQYDVAVKM IKEGSMSEDE FFQEAQTMMK LSHPKLVKFY 60

GVCSKEYPIY IVTEYISNGC LLNYLRSHGK GLEPSQLLEM CYDVCEGMAF LESHQFIHRD 120

LAARNCLVDR DLCVKVSDFG MTRYVLDDQY VSSVGTKFPV KWSAPEVFHY FKYSSKSDVW 180

AFGILMWEVF SLGKQPYDLY DNSQVVLKVS QGHRLYRPHL ASDTIYQIMY SCWHELPEKR 240

PTFQQLLSSI E 251

Keyword

3D-structure; Apoptosis; ATP-binding; Cell adhesion; Complete proteome; Cytoplasm; Direct protein sequencing; Kinase; Metal-binding; Nucleotide-binding; Phosphoprotein; Polymorphism; Reference proteome; SH2 domain; Stress response; Transferase; Tyrosine-protein kinase; Zinc; Zinc-finger.

Sequence Source

Ensembl

Orthology

Ortholog group

Ailuropoda melanoleuca"; ?>Anolis carolinensis"; ?>Bos taurus"; ?>Callithrix jacchus"; ?>Canis familiaris"; ?>Cavia porcellus"; ?>Equus caballus"; ?>Felis catus"; ?>Gallus gallus"; ?>Gorilla gorilla"; ?>Ictidomys tridecemlineatus"; ?>Latimeria chalumnae"; ?>Loxodonta africana"; ?>Macaca mulatta"; ?>Meleagris gallopavo"; ?>Monodelphis domestica"; ?>Mus musculus"; ?>Mustela putorius furo"; ?>Myotis lucifugus"; ?>Nomascus leucogenys"; ?>Ornithorhynchus anatinus"; ?>Oryctolagus cuniculus"; ?>Otolemur garnettii"; ?>Pelodiscus sinensis"; ?>Pongo abelii"; ?>Rattus norvegicus"; ?>Sarcophilus harrisii"; ?>Sus scrofa"; ?>

Gene Ontology

GO:0005829	; C:cytosol
GO:0032587	; C:ruffle membrane
GO:0005524	; F:ATP binding
GO:0046872	; F:metal ion binding
GO:0004715	; F:non-membrane spanning protein tyrosine kinase activity
GO:0005543	; F:phospholipid binding
GO:0004871	; F:signal transducer activity
GO:0007155	; P:cell adhesion
GO:0006921	; P:cellular component disassembly involved in execution phase of apoptosis
GO:0035556	; P:intracellular signal transduction
GO:0007498	; P:mesoderm development
GO:0046777	; P:protein autophosphorylation
GO:0006950	; P:response to stress

KEGG

hsa:660

;

InterPros

IPR011009	; Kinase-like_dom.
IPR011993	; PH_like_dom.
IPR001849	; Pleckstrin_homology.
IPR000719	; Prot_kinase_cat_dom.
IPR017441	; Protein_kinase_ATP_BS.
IPR001245	; Ser-Thr/Tyr_kinase_cat_dom.
IPR000980	; SH2.
IPR008266	; Tyr_kinase_AS.
IPR020635	; Tyr_kinase_cat_dom.
IPR001562	; Znf_Btk_motif.

Pfam

PF00779	; BTK; 1.
PF00169	; PH; 1.
PF07714	; Pkinase_Tyr; 1.
PF00017	; SH2; 1.

SMARTs

SM00107	; BTK; 1.
SM00233	; PH; 1.
SM00252	; SH2; 1.
SM00219	; TyrKc; 1.

Prosites

PS50003	; PH_DOMAIN; 1.
PS00107	; PROTEIN_KINASE_ATP; 1.
PS50011	; PROTEIN_KINASE_DOM; 1.
PS00109	; PROTEIN_KINASE_TYR; 1.
PS50001	; SH2; 1.
PS51113	; ZF_BTK; 1.

Prints

PR00401	; SH2DOMAIN.
PR00402	; TECBTKDOMAIN.
PR00109	; TYRKINASE.

Created Date

20-Feb-2013