EKS-HOS-00043

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EKS-HOS-00043

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
CK1/VRK	386.4	2.9E-115	116	382	267

Status

Reviewed

Ensembl Protein

ENSP00000366210

UniProt Accession

Q8IV63; A6NEG5; A8KA53; Q502Y2; Q9P2V8;

Protein Name

Inactive serine/threonine-protein kinase VRK3

Protein Synonyms/Alias

Serine/threonine-protein pseudokinase VRK3; Vaccinia-related kinase 3;

Gene Name

VRK3

Gene Synonyms/Alias

VRK3;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
ENSG00000105053	ENSP00000414907	ENST00000443401
ENSG00000105053	ENSP00000469325	ENST00000594090
ENSG00000105053	ENSP00000469092	ENST00000596445
ENSG00000105053	ENSP00000468979	ENST00000600137
ENSG00000105053	ENSP00000469802	ENST00000596121
ENSG00000105053	ENSP00000470156	ENST00000601341
ENSG00000105053	ENSP00000471061	ENST00000601912
ENSG00000105053	ENSP00000473222	ENST00000593912
ENSG00000105053	ENSP00000469710	ENST00000593919
ENSG00000105053	ENSP00000471661	ENST00000599342
ENSG00000105053	ENSP00000469400	ENST00000600259
ENSG00000105053	ENSP00000470940	ENST00000597215
ENSG00000105053	ENSP00000469880	ENST00000599538
ENSG00000105053	ENSP00000472217	ENST00000596814
ENSG00000105053	ENSP00000472541	ENST00000594092
ENSG00000105053	ENSP00000473171	ENST00000594948
ENSG00000105053	ENSP00000324636	ENST00000316763
ENSG00000105053	ENSP00000470359	ENST00000601324
ENSG00000105053	ENSP00000366210	ENST00000377011

Organism

Homo sapiens

Functional Description

Inactive kinase that suppresses ERK activity bypromoting phosphatase activity of DUSP3 which specifically dephosphorylates and inactivates ERK in the nucleus (By similarity).

Protein Length

424

Protein Sequence
(FASTA)

MISFCPDCGK SIQAAFKFCP YCGNSLPVEE HVGSQTFVNP HVSSFQGSGS RPPTPKSSPQ 60

KTRKSPQVTR GSPQKTSCSP QKTRQSPQTL KRSRVTTSLE ALPTGTVLTD KSGRQWKLKS 120

FQTRDNQGIL YEAAPTSTLT CDSGPQKQKF SLKLDAKDGR LFNEQNFFQR AAKPLQVNKW 180

KKLYSTPLLA IPTCMGFGVH QDKYRFLVLP SLGRSLQSAL DVSPKHVLSE RSVLQVACRL 240

LDALEFLHEN EYVHGNVTAE NIFVDPEDQS QVTLAGYGFA FRYCPSGKHV AYVEGSRSPH 300

EGDLEFISMD LHKGCGPSRR SDLQSLGYCM LKWLYGFLPW TNCLPNTEDI MKQKQKFVDK 360

PGPFVGPCGH WIRPSETLQK YLKVVMALTY EEKPPYAMLR NNLEALLQDL RVSPYDPIGL 420

PMVP 424

Nucleotide Sequence
(FASTA)

ATGATCTCCT TCTGTCCAGA CTGTGGCAAA AGTATCCAAG CGGCATTCAA ATTCTGCCCC 60

TACTGTGGAA ATTCTTTGCC TGTAGAGGAG CATGTAGGGT CCCAGACCTT TGTCAATCCA 120

CATGTGTCAT CCTTCCAAGG CTCCGGGAGC AGACCCCCAA CCCCCAAAAG CAGCCCTCAG 180

AAGACCAGGA AGAGCCCTCA GGTGACCAGG GGTAGCCCTC AGAAGACCAG CTGTAGCCCT 240

CAGAAGACCA GGCAGAGCCC TCAGACGCTG AAGCGGAGCC GAGTGACCAC CTCACTTGAA 300

GCTTTGCCCA CAGGGACAGT GCTGACAGAC AAGAGTGGGC GACAGTGGAA GCTGAAGTCC 360

TTCCAGACCA GGGACAACCA GGGCATTCTC TATGAAGCTG CACCCACCTC CACCCTCACC 420

TGTGACTCAG GACCACAGAA GCAAAAGTTC TCACTCAAAC TGGATGCCAA GGATGGGCGC 480

TTGTTCAATG AGCAGAACTT CTTCCAGCGG GCCGCCAAGC CTCTGCAAGT CAACAAGTGG 540

AAGAAGCTGT ACTCGACCCC ACTGCTGGCC ATCCCTACCT GCATGGGTTT CGGTGTTCAC 600

CAGGACAAAT ACAGGTTCTT GGTGTTACCC AGCCTGGGGA GGAGCCTTCA GTCGGCCCTG 660

GATGTCAGCC CAAAGCATGT GCTGTCAGAG AGGTCTGTGC TGCAGGTGGC CTGCCGGCTG 720

CTGGATGCCC TGGAGTTCCT CCATGAGAAT GAGTATGTTC ATGGAAATGT GACAGCTGAA 780

AATATCTTTG TGGATCCAGA GGACCAGAGT CAGGTGACTT TGGCAGGCTA TGGCTTCGCC 840

TTCCGCTATT GCCCAAGTGG CAAACACGTG GCCTACGTGG AAGGCAGCAG GAGCCCTCAC 900

GAGGGGGACC TTGAGTTCAT TAGCATGGAC CTGCACAAGG GATGCGGGCC CTCCCGCCGC 960

AGCGACCTCC AGAGCCTGGG CTACTGCATG CTGAAGTGGC TCTACGGGTT TCTGCCATGG 1020

ACAAATTGCC TTCCCAACAC TGAGGACATC ATGAAGCAAA AACAGAAGTT TGTTGATAAG 1080

CCGGGGCCCT TCGTGGGACC CTGCGGTCAC TGGATCAGGC CCTCAGAGAC CCTGCAGAAG 1140

TACCTGAAGG TGGTGATGGC CCTCACGTAT GAGGAGAAGC CGCCCTACGC CATGCTGAGG 1200

AACAACCTAG AAGCTTTGCT GCAGGATCTG CGTGTGTCTC CATATGACCC CATTGGCCTC 1260

CCGATGGTGC CCTAG 1275

Domain Profile

S: 1     wklgkkigkggfGeiyla.....ltckkeekdaklvvkvepkenGpLfvelkfyqraakk  55

         wkl+++++++++G++y+a     ltc+++++++k+++k+++k+ G+Lf+e++f+qraak+

Q: 116   WKLKSFQTRDNQGILYEAaptstLTCDSGPQKQKFSLKLDAKD-GRLFNEQNFFQRAAKP  174

         9*****************9*9999******************9.****************

S: 56    eqikkwkkkkklkllgiPtliasGlhefegekyRflvlprlgrdLqklleqngkr.lsek  114

          q++kwkk ++++ll+iPt++++G+h+   +kyRflvlp+lgr+Lq++l+ ++k+ lse+

Q: 175   LQVNKWKKLYSTPLLAIPTCMGFGVHQ---DKYRFLVLPSLGRSLQSALDVSPKHvLSER  231

         ***************************...*************************99***

S: 115   tvlqlavrlldvleylHeneYvHgdikAanllleeeeasqvyLvdyGlayrycpegkhke  174

         +vlq+a+rlld+le+lHeneYvHg+++A+n+++++e++sqv+L++yG+a+rycp+gkh++

Q: 232   SVLQVACRLLDALEFLHENEYVHGNVTAENIFVDPEDQSQVTLAGYGFAFRYCPSGKHVA  291

         ************************************************************

S: 175   ykedkrraHdGtleftslDaHkGvapsrRsDleiLgYcllkWltgkLPWeallkdpekvq  234

         y+e++r++H+G+lef+s+D+HkG++psrRsDl++LgYc+lkWl+g+LPW+++l+++e+++

Q: 292   YVEGSRSPHEGDLEFISMDLHKGCGPSRRSDLQSLGYCMLKWLYGFLPWTNCLPNTEDIM  351

         ************************************************************

S: 235   kakekfldelpellkkllkkkssseelakyl  265

         k+k+kf+d+++ ++  + ++ ++se+l+kyl

Q: 352   KQKQKFVDKPGPFVGPCGHWIRPSETLQKYL  382

         ******************************8

Domain Sequence
(FASTA)

WKLKSFQTRD NQGILYEAAP TSTLTCDSGP QKQKFSLKLD AKDGRLFNEQ NFFQRAAKPL 60

QVNKWKKLYS TPLLAIPTCM GFGVHQDKYR FLVLPSLGRS LQSALDVSPK HVLSERSVLQ 120

VACRLLDALE FLHENEYVHG NVTAENIFVD PEDQSQVTLA GYGFAFRYCP SGKHVAYVEG 180

SRSPHEGDLE FISMDLHKGC GPSRRSDLQS LGYCMLKWLY GFLPWTNCLP NTEDIMKQKQ 240

KFVDKPGPFV GPCGHWIRPS ETLQKYL 267

Keyword

3D-structure; Alternative splicing; Complete proteome; Nucleotide-binding; Nucleus; Phosphoprotein; Polymorphism; Reference proteome.

Sequence Source

Ensembl

Orthology

Ortholog group

Ailuropoda melanoleuca"; ?>Anolis carolinensis"; ?>Bos taurus"; ?>Callithrix jacchus"; ?>Canis familiaris"; ?>Cavia porcellus"; ?>Danio rerio"; ?>Equus caballus"; ?>Felis catus"; ?>Gallus gallus"; ?>Gasterosteus aculeatus"; ?>Gorilla gorilla"; ?>Ictidomys tridecemlineatus"; ?>Latimeria chalumnae"; ?>Loxodonta africana"; ?>Macaca mulatta"; ?>Meleagris gallopavo"; ?>Monodelphis domestica"; ?>Mus musculus"; ?>Mustela putorius furo"; ?>Myotis lucifugus"; ?>Nomascus leucogenys"; ?>Oreochromis niloticus"; ?>Oryctolagus cuniculus"; ?>Oryzias latipes"; ?>Otolemur garnettii"; ?>Pan troglodytes"; ?>Pelodiscus sinensis"; ?>Rattus norvegicus"; ?>Sarcophilus harrisii"; ?>Sus scrofa"; ?>Taeniopygia guttata"; ?>Takifugu rubripes"; ?>Tarsius syrichta"; ?>Tetraodon nigroviridis"; ?>Tursiops truncatus"; ?>Xenopus tropicalis"; ?>Xiphophorus maculatus"; ?>

Gene Ontology

GO:0005634	; C:nucleus
GO:0005524	; F:ATP binding
GO:0004672	; F:protein kinase activity
GO:0070373	; P:negative regulation of ERK1 and ERK2 cascade
GO:0032516	; P:positive regulation of phosphoprotein phosphatase activity

KEGG

hsa:51231

;

InterPros

IPR011009	; Kinase-like_dom.
IPR000719	; Prot_kinase_cat_dom.

Pfam

PF00069

; Pkinase; 1.

SMARTs

Prosites

PS00107	; PROTEIN_KINASE_ATP; FALSE_NEG.
PS50011	; PROTEIN_KINASE_DOM; 1.
PS00108	; PROTEIN_KINASE_ST; FALSE_NEG.

Prints

Created Date

20-Feb-2013